Proteins

ABSTRACT

Novel protein antigens from Group B Streptococcus are described, together with the nucleic acid sequences encoding them. The use of vaccines and screening methods is also described.

[0001] The present invention relates to proteins derived fromStreptococcus agalactiae, nucleic acid molecules encoding such proteins,and the use of the proteins as antigens and/or immunogens and indetection/diagnosis. It also relates to a method for the rapid screeningof bacterial genomes to isolate and characterise bacterial cell envelopeassociated or secreted proteins.

[0002] The Group B Streptococcus (GBS) (Streptococcus agalactiae) is anencapsulated bacterium which emerged in the 1970s as a major pathogen ofhumans causing sepsis and meningitis in neonates as well as adults. Theincidence of early onset neonatal infection during the first 5 days oflife varies from 0.7 to 3.7 per 1000 live births and causes mortality inabout 20% of cases. Between 25-50% of neonates surviving early onsetinfections frequently suffer neurological sequalae. Late onset neonatalinfections occur from 6 days to three months of age at a rate of about0.5 - 1.0 per 1000 live births.

[0003] There is an established association between the colonisation ofthe maternal genital tract by GBS at the time of birth and the risk ofneonatal sepsis. In humans it has been established that the rectum mayact as a reservoir for GBS. Susceptibility in the neonate is correlatedwith the a low concentration or absence of IgG antibodies to thecapsular polysaccharides found on GBS causing human disease. In the USAstrains isolated from clinical cases usually belong to capsularserotypes Ia, Ib, II, III although serotype V may be of increasingsignificance. Type VIII GBS is the major cause of neonatal sepsis inJapan.

[0004] A possible means of prevention involves intra or postpartumadministration of antibiotics to the mother but there are concerns thatthis might lead to the emergence of resistant organisms and in somecases allergic reactions. Vaccination of the adolescent females toinduce long lasting maternally derived immunity is one of the mostpromising approaches to prevent GBS infections in neonates. The capsularpolysaccharide antigens of these organisms have attracted most attentionas with regard to vaccine development. Studies in healthy adultvolunteers have shown that serotype Ia, II and III polysaccharides arenon-toxic and immunogenic in approximately 65%, 95% and 70% ofnon-immune adults respectively. One of the problems with using capsuleantigens as vaccines is that the response rates vary according topre-immunisation status and the polysaccharide antigen and not allvaccinees produce adequate levels of IgG antibody as indicated invaccination studies with GBS polysaccharides in human volunteers.

[0005] Some people do not respond despite repeated stimuli. Theseproperties are due to the T-independent nature of polysaccharideantigens. One strategy to enhance the immunogenicity of these vaccinesis to enhance the T cell dependent properties of polysaccharides byconjugating them to a protein. The use of polysaccharide conjugateslooks promising but there are still unresolved questions concerning thenature of the carrier protein. A conjugate vaccine against GBS wouldrequire at least 4 different conjugates to be prepared adding to thecost of a vaccine.

[0006] Approaches to vaccination against GBS infections which rely onthe use of capsular polysaccharides have the disadvantage that responserates are likely to vary considerably according to pre-immunisationstatus and the particular type of polysaccharide antigen used. Resultsof trials with conjugate vaccines in human volunteers have indicatedthat response rates may only be around 65% for some of the key capsuleantigens (Larsson et al., Infection and Immunity 64:3518-3523 (1996)).It is also not clear whether all individuals responding to the vaccinewould have adequate levels of polysaccharide specific IgG which cancross the placenta and afford immunity to neonates. By conjugating aprotein carrier to the polysaccharide antigen it may be possible toconvert them to T-cell dependent antigens and enhance theirimmunogenicity.

[0007] Preliminary studies with GBS type III polysaccharide-tetanustoxoid conjugate have been encouraging (Baker et al., Reviews ofInfectious Diseases 7:458-467 (1985), Baker et al., The New EnglandJournal of Medicine 319:1180-1185 (1988), Paoletti et al., Infection andImmunity 64:677-679 (1996), Paoletti et al., Infection and Immunity62:3236-3243 (1994)) but in developed countries the use of tetanus maybe disadvantageous since most adults will have been immunised againsttetanus within the past five years. Additional boosters with tetanustoxoid may cause adverse reactions (Boyer., Current Opinions inPediatrics 7:13-18 (1995)). The polysaccharide conjugate vaccines havethe disadvantage of being costly to produce and manufacture incomparison with many other kinds of vaccines. There is also the possiblerisk of problems caused by the cross reactivity between GBSpolysaccharides and sialic acid-containing human glycoproteins.

[0008] Recent evidence suggests that bacterial surface proteins also maybe useful to confer immunity. A protein called Rib which is found onmost serotype III strains but rarely on serotypes Ia, Ib or II confersimmunity to challenge with Rib expressing GBS in animal models(Stalhammar-Carlemalm et al., Journal of Experimental Medicine177:1593-1603 (1993)). Another surface protein of interest as acomponent of a vaccine is the alpha antigen of the C proteins whichprotected vaccinated mice against lethal infection with strainsexpressing alpha protein. The amount of this antigen expressed by GBSstrains varies markedly, however an alternative to polysaccharides asantigens is the use of protein antigens derived from GBS. Recentevidence suggest that the GBS surface associated proteins Rib and alphaC protein may be used to confer immunity to GBS infections inexperimental model systems (Stalhammar-Carlemalm et al., (1993) [supra],Larsson et al., (1996) [supra]). However these two proteins are notconserved in all serotypes of GBS which cause disease in humans.Assuming that these antigens would be immunogenic and elicit protectivelevel responses in humans they would not confer protection against allinfections caused by GBS as 10% of infectious Group B streptococci donot express Rib or C protein alpha.

[0009] This invention seeks to overcome the problem of vaccinationagainst GBS by using a novel screening method specifically designed toidentify those Group B Streptococcus genes encoding bacterial cellsurface associated or secreted proteins. The proteins expressed by thesegenes may be immunogenic, and therefore may be useful in the preventionand treatment of Group B Streptococcus infection. For the purposes ofthis application, the term immunogenic means that these proteins willelicit a protective immune response within a subject. Using this novelscreening method a number of genes encoding novel Group B Streptococcusproteins have been identified.

[0010] Thus in a first aspect, the present invention provides a Group BStreptococcus protein, polypeptide or peptide having a sequence selectedfrom those shown in FIG. 1, or fragments or derivatives thereof.

[0011] It will be apparent to the skilled person that proteins andpolypeptides included within this group may be cell surface receptors,adhesion molecules, transport proteins, membrane structural proteins,and/or signalling molecules.

[0012] Alterations in the amino acid sequence of a protein can occurwhich do not affect the function of a protein. These include amino aciddeletions, insertions and substitutions and can result from alternativesplicing and/or the presence of multiple translation start sites andstop sites. Polymorphisms may arise as a result of the infidelity of thetranslation process. Thus changes in amino acid sequence may betolerated which do not affect the protein's function.

[0013] Thus, the present invention includes derivatives or variants ofthe proteins, polypeptides, and peptides of the present invention whichshow at least 50% identity to the proteins, polypeptides and peptidesdescribed herein. Preferably the degree of sequence identity is at least60% and preferably it is above 75%. More preferably still it is above80%, 90% or even 95%.

[0014] The term identity can be used to describe the similarity betweentwo polypeptide sequences. A software package well known in the art forcarrying out this procedure is the CLUSTAL program. It compares theamino acid sequences of two polypeptides and finds the optimal alignmentby inserting spaces in either sequence as appropriate. The amino acididentity or similarity (identity plus conservation of amino acid type)for an optimal alignment can also be calculated using a software packagesuch as BLASTx. This program aligns the largest stretch of similarsequence and assigns a value to the fit. For any one pattern comparisonseveral Legions of similarity may be found, each having a differentscore. One skilled in the art will appreciate that two polypeptides ofdifferent lengths may be compared over the entire length of the longerfragment. Alternatively small regions may be compared. Normallysequences of the same length are compared for a useful comparison to bemade.

[0015] Manipulation of the DNA encoding the protein is a particularlypowerful technique for both modifying proteins and for generating largequantities of protein for purification purposes. This may involve theuse of PCR techniques to amplify a desired nucleic acid sequence. Thusthe sequence data provided herein can be used to design primers for usein PCR so that a desired sequence can be targeted and then amplified toa high degree.

[0016] Typically primers will be at least five nucleotides long and willgenerally be at least ten nucleotides long (e.g. fifteen to twenty-fivenucleotides long). In some cases primers of at least thirty or at leastthirty-five nucleotides in length may be used.

[0017] As a further alternative chemical synthesis may be used. This maybe automated. Relatively short sequences may be chemically synthesisedand ligated together to provide a longer sequence.

[0018] Thus in a further aspect, the present invention provides, anucleic acid molecule comprising or consisting of a sequence which is:

[0019] (i) any of the DNA sequences set out in FIG. 1 herein or theirRNA equivalents;

[0020] (ii) a sequence which is complementary to any of the sequences of(i);

[0021] (iii) a sequence which codes for the same protein or polypeptide,as those sequences of (i) or (ii);

[0022] (iv) a sequence which is shows substantial identity with any ofthose of (i), (ii) and (iii); or

[0023] (v) a sequence which codes for a derivative or fragment of anucleic acid molecule shown in FIG. 1.

[0024] The term identity can also be used to describe the similaritybetween two individual DNA sequences. The ‘bestfit’ program (Smith andWaterman, Advances in applied Mathematics, 482-489 (1981)) is oneexample of a type of computer software used to find the best segment ofsimilarity between two nucleic acid sequences, whilst the GAP programenables sequences to be aligned along their whole length and finds theoptimal alignment by inserting spaces in either sequence as appropriate.

[0025] The present invention includes nucleic acid sequences which showat least 50% identity to the nucleic acid sequences described herein.Preferably the degree of sequence identity is at least 60% andpreferably it is above 75%. More preferably still it is above 80%, 90%or even 95%.

[0026] The term ‘RNA equivalent’ when used above indicates that a givenRNA molecule has a sequence which is complementary to that of a givenDNA molecule, allowing for the fact that in RNA ‘U’ replaces ‘T’ in thegenetic code. The nucleic acid molecule may be in isolated, recombinantor chemically synthetic form.

[0027] DNA constructs can readily be generated using methods well knownin the art. These techniques are disclosed, for example in J. Sambrooket al, Molecular Cloning 2^(nd) Edition, Cold Spring Harbour LaboratoryPress (1989). Modifications of DNA constructs and the proteins expressedsuch as the addition of promoters, enhancers, signal sequences, leadersequences, translation start and stop signals and DNA stabilitycontrolling regions, or the addition of fusion partners may then befacilitated.

[0028] Normally the DNA construct will be inserted into a vector whichmay be any suitable vector, including plasmid, virus, bacteriophage,transposon, minichromosome, liposome or mechanical carrier. Theexpression vectors of the invention are DNA constructs suitable forexpressing DNA which encodes the desired protein product which mayinclude: (a) a regulatory element (e.g. a promoter, operator, activator,repressor and/or enhancer), (b) a structural or coding sequence which istranscribed into mRNA and (c) appropriate transcription, translation,initiation and termination sequences. The vector may further comprise aselectable marker, for example antibiotic resistance, which facilitatesthe selection and/or identification of cells containing the vector.

[0029] Expression of the protein is achieved by the transformation ortransfection of the vector into a host cell which may be of eukaryoticor prokaryotic origin. For the production of recombinant protein,expression may be inducible expression or expression only in certaintypes of cells or both inducible and cell-specific. Particularlypreferred among inducible vectors are vectors that can be induced forexpression by environmental factors that are easy to manipulate, such astemperature and nutrient additives. A variety of suitable vectors,including constitutive and inducible expression vectors for use inprokaryotic and eukaryotic hosts, are well known and employed routinelyby those skilled in the art.

[0030] A great variety of expression vectors can be used to express theGroup B Streptococcus protein(s) of the invention. Such vectors include,among others, chromosomal, episomal and virus-derived vectors, forexample, vectors derived from bacterial plasmids, from bacteriophage,from transposons, from yeast elements, from viruses such asbaculoviruses, papova viruses, such as SV40, vaccinia viruses,adenoviruses and retroviruses, and vectors derived from combinationsthereof, such as those derived from plasmid and bacteriophage geneticelements, such as cosmids and phagemids, all may be used in accordancewith the invention. Generally, any vector suitable to maintain,propagate or express. nucleic acid to express a polypeptide in a hostmay be used for expression in this regard. Such vectors thus form yet afurther aspect of the invention.

[0031] The appropriate DNA sequence may be inserted into the vector byany of a variety of well-known and routine techniques.

[0032] The nucleic acid sequence in the expression vector is operativelylinked to appropriate expression control sequence(s) including, forinstance, a promoter to direct mRNA transcription. Representatives ofsuch promoters include, but are not limited to, the phage lambda PLpromoter, the T3 and T7 promoters, the E. coli lac, trp, tac, and λP_(L)promoters, the microbial eukaryote GAL, glucoamylase andcellobiohydrolase promoters and the mammalian metallothionein (mouse)and heat-shock (human) promoters.

[0033] In general, expression vectors will contain sites fortranscription initiation and termination, and, in the transcribedregion, a ribosome binding site for translation. The coding portion ofmature transcripts expressed by the constructs will generally include atranslation initiating AUG at the beginning and a termination codonappropriately positioned at the end of the polypeptide to be translated.

[0034] Representative examples of appropriate hosts for recombinantexpression of the Group B Streptococcus protein(s) of the inventioninclude bacterial cells, such as streptococci, staphylococci, E. coli,streptomyces and Bacillus subtilis cells; fungal cells, such as yeastcells and Aspergillus cells; insect cells such as Drosophila S2 andSpodoptera Sf9 cells; animal cells such as CHO, COS, HeLa and Bowesmelanoma cells; and plant cells. Such host cells form yet a furtheraspect of the present invention.

[0035] Microbial cells employed in the expression of proteins can bedisrupted by any convenient method, including freeze-thaw cycling,sonication, mechanical disruption, or use of cell lysing agent, suchmethods which are known to those skilled in the art.

[0036] The polypeptide can be recovered and purified from recombinantcell cultures by well-known methods including ammonium sulphate orethanol precipitation, acid extraction, anion or cation exchangechromatography, phosphocellulose, chromatography, hydrophobicinteraction chromatography, affinity chromatography, hydroxylapatitechromatography and lectin chromatography. Well known techniques forrefolding protein may be employed to regenerate active conformation whenthe polypeptide is denatured during isolation and or purification.

[0037] The Group B Streptococcus proteins described herein canadditionally be used as target antigens to raise antibodies, or togenerate affibodies. These can be used to detect Group B Streptococcus.

[0038] Thus in a further aspect the present invention provides, anantibody, affibody, or a derivative thereof which binds to any one ormore of the proteins, polypeptides, peptides, fragments or derivativesthereof, as described herein.

[0039] Antibodies within the scope of the present invention may bemonoclonal or polyclonal. Polyclonal antibodies can be raised bystimulating their production in a suitable animal host (e.g. a mouse,rat, guinea pig, rabbit, sheep, goat or monkey) when a protein asdescribed herein, or a homologue, derivative or fragment thereof, isinjected into the animal. If desired, an adjuvant may be administeredtogether with the protein. Well-known adjuvants include Freund'sadjuvant (complete and incomplete) and aluminium hydroxide. Theantibodies can then be purified by virtue of their binding to a proteinas described herein and by many other means well-known to those skilledin the art.

[0040] Monoclonal antibodies can be produced from hybridomas. These canbe formed by fusing myeloma cells and spleen cells which produce thedesired antibody in order to form an immortal cell line. Thus thewell-known Kohler & Milstein technique (Nature 256 (1975)) or subsequentvariations upon this technique can be used.

[0041] Techniques for producing monoclonal and polyclonal antibodiesthat bind to a particular polypeptide/protein are now well developed inthe art. They are discussed in standard immunology textbooks, forexample in Roitt et al, Immunology second edition (1989), ChurchillLivingstone, London.

[0042] In addition to whole antibodies, the present invention includesderivatives thereof which are capable of binding to proteins etc asdescribed herein. Thus the present invention includes antibody fragmentsand synthetic constructs. Examples of antibody fragments and syntheticconstructs are given by Dougall et al., Tibtech 12 372-379 (September1994).

[0043] Antibody fragments include, for example, Fab, F(ab′)₂ and Fvfragments. Fv fragments can be modified to produce a synthetic constructknown as a single chain Fv (scFv) molecule. This includes a peptidelinker covalently joining V_(h) and V_(l) regions, which contributes tothe stability of the molecule. Other synthetic constructs that can beused include CDR peptides. These are synthetic peptides comprisingantigen-binding determinants. Peptide mimetics may also be used. Thesemolecules are usually conformationally restricted organic rings thatmimic the structure of a CDR loop and that include antigen-interactiveside chains.

[0044] Synthetic constructs include chimaeric molecules. Thus, forexample, humanised (or primatised) antibodies or derivatives thereof arewithin the scope of the present invention. An example of a humanisedantibody is an antibody having human framework regions, but rodenthypervariable regions. Ways of producing chimaeric antibodies arediscussed for example by Morrison et al in PNAS, 81, 6851-6855 (1984)and by Takeda et al in Nature. 314, 452-454 (1985).

[0045] Synthetic constructs also include molecules comprising anadditional moiety that provides the molecule with some desirableproperty in addition to antigen binding. For example the moiety may be alabel (e.g. a fluorescent or radioactive label). Alternatively, it maybe a pharmaceutically active agent.

[0046] Affibodies are proteins which are found to bind to targetproteins with a low dissociation constant. They are selected from phagedisplay libraries expressing a segment of the target protein of interest(Nord K, Gunneriusson E, Ringdahl J, Stahl S, Uhlen M, Nygren Pa.,Department of Biochemistry and Biotechology, Royal Institute ofTechnology (KTH), Stockholm, Sweden).

[0047] In a further aspect the invention provides an immunogeniccomposition comprising one or more proteins, polypeptides, peptides,fragments or derivatives thereof, or nucleotide sequences describedherein. The immunogenic composition may include nucleic acid sequencesID-65 and/or ID-66 as described herein. Alternatively, the immunogeniccomposition may comprise proteins/polypeptides including ID-65, ID-83,ID-89, ID-93 and/or ID-96 as described herein, or fragments orderivatives thereof. A composition of this sort may be useful in thetreatment or prevention of Group B Streptococcus infection in subject.In a preferred aspect of the invention the immunogenic composition is avaccine.

[0048] In other aspects the invention provides:

[0049] i) Use of an immunogenic composition as described herein in thepreparation of a medicament for the treatment or prophylaxis of Group BStreptococcus infection. Preferably the medicament is a vaccine.

[0050] ii) A method of detection of Group B Streptococcus whichcomprises the step of bringing into contact a sample to be tested withat least one antibody, affibody, or a derivative thereof, as describedherein.

[0051] iii) A method of detection of Group B Streptococcus whichcomprises the step of bringing into contact a sample to be tested withat least one protein, polypeptide, peptide, fragments or derivatives asdescribed herein.

[0052] iv) A method of detection of Group B Streptococcus whichcomprises the step of bringing into contact a sample to be tested withat least one nucleic acid molecule as described herein.

[0053] v) A kit for the detection of Group B Streptococcus comprising atleast one antibody, affibody, or derivatives thereof, described herein.

[0054] vi) A kit for the detection of Group B Streptococcus comprisingat least one Group B Streptococcus protein, polypeptide, peptide,fragment or derivative thereof, as described herein.

[0055] vii) A kit for the detection of Group B Streptococcus comprisingat least one nucleic acid of the invention.

[0056] As described previously, the novel proteins described herein areidentified and isolated using a screening method which specificallyidentifies those Group B Streptococcus genes encoding bacterial cellenvelope associated or secreted proteins.

[0057] Given that the inventors have identified a group of importantproteins, such proteins are potential targets for anti-microbialtherapy. It is necessary, however, to determine whether each individualprotein is essential for the organism's viability. Thus, the presentinvention also provides a method of determining whether a protein orpolypeptide as described herein represents a potential anti-microbialtarget which comprises inactivating said protein and determining whetherGroup B Streptococcus is still viable.

[0058] A suitable method for inactivating the protein is to effectselected gene knockouts, ie prevent expression of the protein anddetermine whether this results in a lethal change. Suitable methods forcarrying out such gene knockouts are described in Li et al, P.N.A.S.,94:13251-13256 (1997) and Kolkman et al., Journal of BiologicalChemistry 272: 19502-19508 (1997); Kolkman et al., Journal ofBacteriology 178: 3736-3741 (1996).

[0059] In a final aspect the present invention provides the use of anagent capable of antagonising, inhibiting or otherwise interfering withthe function or expression of a protein or polypeptide of the inventionin the manufacture of a medicament for use in the treatment orprophylaxis of Group B Streptococcus infection.

[0060] The invention will now be described by means of the followingexamples which should not in any way be construed as limiting. Theexamples refer to the figures in which:

[0061]FIG. 1: (A) Shows a number of full length nucleotide sequencesencoding antigenic Group B Streptococcus proteins and the correspondingamino acid sequences.

[0062]FIG. 2: Shows the results of vaccine trials using the proteinsID-65 and ID-66;

[0063]FIG. 3: Shows a number of oligonucleotide primers used in thescreening process

[0064] nucS1 primer designed to amplify a mature form of the nuc A gene

[0065] nucS2—primer designed to amplify a mature form of the nuc A gene.

[0066] nucS3 primer designed to amplify a mature form of the nuc A gene

[0067] nucR primer designed to amplify a mature form of the nuc A gene

[0068] nucseq primer designed to sequence DNA cloned into the pTREP-Nucvector

[0069] pTREPF nucleic acid sequence containing recognition site forECORV. Used for cloning fragments into pTREX7.

[0070] pTREPR nucleic acid sequence containing recognition site forBAMH 1. Used for cloning fragments into pTREX7.

[0071] PUCF forward sequencing primer, enables direct sequencing ofcloned DNA fragments.

[0072] VR example of gene specific primer used to obtain further antigenDNA sequence by the method of DNA walking.

[0073] V1 example of gene specific primer used to obtain further antigenDNA sequence by the method of DNA walking.

[0074] V2 example of gene specific primer used to obtain further antigenDNA sequence by the method of DNA walking.

[0075]FIG. 4: (i) Schematic presentation of the nucleotide sequence ofthe unique gene cloning site immediately upstream of the mature nuc genein pTREP1-nuc1, pTREP1-nuc2 and pTREP1-nuc3. Each of the pTREP-nucvectors contain an EcoRV (a Smal site in pTREP1-nuc2) cleavage sitewhich allows cloning of genomic DNA fragments in 3 different frames withrespect to the mature nuc gene.

[0076] (ii) A physical and genetic summary map of the pTREP1-nucvectors. The expression cassette incorporating nuc, the macrolides,lincosamides and streptogramin B (MLS) resistance determinant, and thereplicon (rep) Ori-pAMβ1 are depicted (not drawn to scale).

[0077] (iii) Schematic presentation of the expression cassette showingthe various sequence elements involved in gene expression and locationof unique restriction endonuclease sites (not drawn to scale).

[0078]FIG. 5: SDS-PAGE analysis of a purified preparation of theHis-tagged ID-65 and ID-83 protein antigens (predicted molecular weightsof 57,144 and 25,000 daltons respectively) on a 12% polyacrylamide gel.Lanes: MW, molecular weight standards; 1, His-tagged ID-65 protein; 2,His-tagged ID-83 protein

[0079]FIG. 6: SDS PAGE analysis of a purified preparation of theHis-tagged ID-93 protein antigen (predicted molecular weight=28,000daltons) on a 12% polyacrylamide gel. Lanes: MW, molecular weightstandards; 1, His-tagged ID-93 protein.

[0080]FIG. 7: SDS PAGE analysis of a purified preparation of theHis-tagged ID-89 and ID-96 protein antigens (predicted molecular weightsof 35,000 and 31,000 daltons respectively) on a 12% polyacrylamide gel.Lanes: MW, molecular weight standards; 1, His-tagged ID-89 protein; 2,His-tagged ID-96 protein.

[0081]FIG. 8: IgG Titres against the ID-65 and ID-83 proteins

[0082] 1=ID-65+Alum Group−Bleed at 5 weeks

[0083] 2=PBS+Alum Control Group−Bleed at 5 weeks

[0084] (For groups 1 and 2, ELISAs were performed on purified ID-65protein)

[0085] 3=ID-83+Alum Group−Bleed at 5 weeks

[0086] 4=PBS+Alum Control Group−Bleed at 5 weeks

[0087] (For groups 3 and 4, ELISAs were performed on purified ID-83protein)

[0088]FIG. 9: Shows the results of vaccine trials using the proteinID-93.

[0089]FIG. 10: IgG titres against the ID-93 protein.

[0090] 1=ID-93+Alum Group−Bleed at 3 weeks

[0091] 2=ID-93+Alum Group−Bleed at 6 weeks

[0092] 3=PBS+Alum Control Group−Bleed at 3 weeks

[0093] 4=PBS+Alum Control Group−Bleed at 6 weeks

[0094]FIG. 11: IgG titres against the ID-89 and ID-96 proteins

[0095] 1=ID-89+TitreMax Gold Group−Bleed at 3 weeks

[0096] 2=ID-89+TitreMax Gold−Bleed at 6 weeks

[0097] 3=PBS+TitreMax Gold Control Group−Bleed at 3 weeks

[0098] 4=PBS+TitreMax Gold Control Group−Bleed at 6 weeks

[0099] 5=ID-96+TitreMax Gold Group−Bleed at 3 weeks

[0100] 6=ID-96+TitreMax Gold Group−Bleed at 6 weeks

[0101] 7=PBS+TitreMax Gold Control Group−Bleed at 3 weeks

[0102] 8=PBS+TitreMax Gold Control Group−Bleed at 6 weeks

[0103] For Groups 1-4, ELISAs were performed on purified ID-89 protein.

[0104] For Groups 5-6, ELISAs were performed on purified ID-96 protein.

[0105]FIG. 12: Southern blot analysis of genomic DNA. Genomic DNA fromeach of the strains listed in Table 7 was digested completely with HinDIII (NEB) and electrophoresed at 40 Volts for 6 hours in 0.8% agarose,transferred onto Hybond N⁺ (Amersham) membrane by Southern blot andhybridised with the digoxigenin-labelled rib gene probe. Specificallybound DNA probe was identified using the DIG Nucleic Acid Detection Kit(Boehringer Mannheim).

[0106]FIG. 13: Southern blot analysis of genomic DNA. Genomic DNA fromeach of the strains listed in Table 6 was digested completely with HinDIII (NEB) and electrophoresed at 40 Volts for 6 hours in 0.8% agarose,transferred onto Hybond N⁺ (Amersham) membrane by Southern blot andhybridised with the digoxigenin-labelled ID-65 gene probe. Specificallybound DNA probe was identified using the DIG Nucleic Acid Detection Kit(Boehringer Mannheim).

[0107]FIG. 14: Southern blot analysis of genomic DNA. Genomic DNA fromeach of the strains listed in Table 6 was digested completely with HinDIII (NEB) and electrophoresed at 40 Volts for 6 hours in 0.8% agarose,transferred onto Hybond N⁺ (Amersham) membrane by Southern blot andhybridised with the digoxigenin-labelled ID-89 gene probe. Specificallybound DNA probe was identified using the DIG Nucleic Acid Detection Kit(Boehringer Mannheim).

[0108]FIG. 15: Southern blot analysis of genomic DNA. Genomic DNA fromeach of the strains listed in Table 6 was digested completely with HinDIII (NEB) and electrophoresed at 40 Volts for 6 hours in 0.8% agarose,transferred onto Hybond N⁺ (Amersham) membrane by Southern blot andhybridised with the digoxigenin-labelled ID-93 gene probe. Specificallybound DNA probe was identified using the DIG Nucleic Acid Detection Kit(Boehringer Mannheim).

[0109]FIG. 16: Southern blot analysis of genomic DNA. Genomic DNA fromeach of the strains listed in Table 6 was digested completely with EcoRI (NEB) and electrophoresed at 40 Volts for 6 hours in 0.8% agarose,transferred onto Hybond N⁺ (Amersham) membrane by Southern blot andhybridised with the digoxigenin-labelled ID-96 gene probe. Specificallybound DNA probe was identified using the DIG Nucleic Acid Detection Kit(Boehringer Mannheim).

EXAMPLE 1

[0110] Gene/partial gene sequences putatively encoding exported proteinsin S. agalactiae have been identified, unless stated otherwise, usingthe nuclease screening system described herein vis, the LEEP(Lactococcus Expression of Exported Proteins) system. These have beenfurther analysed to remove artefacts. The nucleotide sequences of genesidentified using the screening system have been characterised using anumber of parameters described below.

[0111] 1. All putative surface proteins are analysed for leader/signalpeptide sequences. Bacterial signal peptide sequences share a commondesign. They are characterised by a short positively charged N-terminus(N region) immediately preceding a stretch of hydrophobic residues(central portion-h region) followed by a more polar C-terminal portionwhich contains the cleavage site (c-region). Computer software is usedto perform hydropathy profiling of putative proteins (Marcks, Nuc. Acid.Res., 16:1829-1836 (1988)) which is used to identify the distinctivehydrophobic portion (h-region) typical of leader peptide sequences. Inaddition, the presence/absence of a potential ribosomal binding site(Shine-Dalgarno sequence required for translation) is also noted.

[0112] 2. All putative surface protein sequences are used to search theOWL sequence database which includes a translation of the GENBANK andSWISSPROT database.. This allows identification of similar sequenceswhich may have been previously characterised not only at the sequencelevel but at a functional level. It may also provide informationindicating that these proteins are indeed surface related and notartefacts.

[0113] 3. Putative S. agalactiae surface proteins are also assessed fortheir novelty. Some of the identified proteins may or may not possess atypical leader peptide sequence and may not show homology with anyDNA/protein sequences in the database. Indeed these proteins mayindicate the primary advantage of our screening method, i.e. isolatingatypical surface-related proteins, which would have been missed in allpreviously described screening protocols.

[0114] The construction of three reporter vectors and their use in L.lactis to identify and isolate genomic DNA fragments from pathogenicbacteria encoding secreted or surface associated proteins is nowdescribed.

[0115] Construction of the pTREP1-nuc series of reporter vectors

[0116] (a) Construction of expression plasmid PTREP1

[0117] The pTREP1 plasmid is a high-copy number (40-80 per cell)theta-replicating gram positive plasmid, which is a derivative of thepTREX plasmid which is itself a derivative of the previously publishedpIL253 plasmid. pIL253 incorporates the broad Gram-positive host rangereplicon of pAMβ1 (Simon and Chopin, Biochemie 70: 559-566 (1988))Llactis sex-factor. pIL253 also lacks the tra function which is necessaryfor transfer or efficient mobilisation by conjugative parent plasmidsexemplified by pIL501. The Enterococcal pAMβ1 replicon has previouslybeen transferred to various species including Streptococcus,Lactobacillus and Bacillus species as well as Clostridiumacetobutylicum, (LeBlanc et al., Proceedings of the National Academy ofScience USA 75:3484-3487 (1978)) indicating the potential broad hostrange utility. The pTREP1 plasmid represents a constitutivetranscription vector.

[0118] The pTREX vector was constructed as follows. An artificial DNAfragment containing a putative RNA stabilising sequence, a translationinitiation region (TIR), a multiple cloning site for insertion of thetarget genes and a transcription terminator was created by annealing 2complementary oligonucleotides and extending with Tfl DNA polymerase.The sense and anti-sense oligonucleotides contained the recognitionsites for NheI and BamHI at their 5′ ends respectively to facilitatecloning. This fragment was cloned between the XbaI and BamHI sites inpUC19NT7, a derivative of pUC19 which contains the T7 expressioncassette from pLET1 (Wells et al., J. Appl. Bacteriol. 74:629-636(1993)) cloned between the EcoRI and HindIII sites. The resultingconstruct was designated pUCLEX. The complete expression cassette ofpUCLEX was then removed by cutting with HindIII and blunting followed bycutting with EcoRI before cloning into EcoRI and SacI (blunted) sites ofpIL253 to generate the vector pTREX (Wells and Schofield, In Currentadvances in metabolism, genetics and applications-NATO ASI Series. H98:37-62. (1996)). The putative RNA stabilising sequence and TIR arederived from the Escherichia coli T7 bacteriophage sequence and modifiedat one nucleotide position to enhance the complementarity of the ShineDalgarno (SD) motif to the ribosomal 16s RNA of Lactococcus lactis(Schofield et al. pers. coms. University of Cambridge Dept. Pathology.).

[0119] A Lactococcus lactis MG1363 chromosomal DNA fragment exhibitingpromoter activity which was subsequently designated P7 was clonedbetween the EcoRI and BglII sites present in the expression cassette,creating pTREX7. This active promoter region had been previouslyisolated using the promoter probe vector pSB292 (Waterfield et al., Gene165:9-15 (1995)). The promoter fragment was amplified by PCR using theVent DNA polymerase according to the manufacturer.

[0120] The pTREP1 vector was then constructed as follows. An artificialDNA fragment which included a transcription terminator, the forward pUCsequencing primer, a promoter multiple cloning site region and auniversal translation stop sequence was created by annealing twooverlapping partially complementary synthetic oligonucleotides togetherand extending with sequenase according to manufacturers instructions.The sense and anti-sense (pTREP_(F) and pTREP_(R)) oligonucleotidescontained the recognition sites for EcoRV and BamHI at their 5′ endsrespectively to facilitate cloning into pTREX7. The transcriptionterminator was that of the Bacillus penicillinase gene, which has beenshown to be effective in Lactococcus (Jos et al., Applied andEnvironmental Microbiology 50:540-542 (1985)). This was considerednecessary as expression of target genes in the pTREX vectors wasobserved to be leaky and is thought to be the result of cryptic promoteractivity in the origin region (Schofield et al. pers. coms. Universityof Cambridge Dept. Pathology.). The forward pUC primer sequencing wasincluded to enable direct sequencing of cloned DNA fragments. Thetranslation stop sequence which encodes a stop codon in 3 differentframes was included to prevent translational fusions between vectorgenes and cloned DNA fragments. The pTREX7 vector was first digestedwith EcoRI and blunted using the 5′-3′ polymerase activity of T4 DNApolymerase (NEB) according to manufacturer's instructions. The EcoRIdigested and blunt ended pTREX7 vector was then digested with Bgl IIthus removing the P7 promoter. The artificial DNA fragment derived fromthe annealed synthetic oligonucleotides was then digested with EcoRV andBam HI and cloned into the EcoRI(blunted)-Bgl II digested pTREX7 vectorto generate pTREP. A Lactococcus lactis MG1363 chromosomal promoterdesignated P1 was then cloned between the EcoRI and BglII sites presentin the pTREP expression cassette forming pTREP1. This promoter was alsoisolated using the promoter probe vector pSB292 and characterised byWaterfield et al., (1995) [supra]. The P1 promoter fragment wasoriginally amplified by PCR using vent DNA polymerase according tomanufacturers instructions and cloned into the pTREX as an EcoRI-BglIIDNA fragment. The EcoRI-BglII P1 promoter containing fragment wasremoved from pTREX1 by restriction enzyme digestion and used for cloninginto pTREP (Schofield et al. pers. coms. University of Cambridge, Dept.Pathology.).

[0121] (b) PCR amplification of the S. aureus nuc gene.

[0122] The nucleotide sequence of the S. aureus nuc gene (EMBL databaseaccession number V01281) was used to design synthetic oligonucleotideprimers for PCR amplification. The primers were designed to amplify themature form of the nuc gene designated nucA which is generated byproteolytic cleavage of the N-terminal 19 to 21 amino acids of thesecreted propeptide designated Snase B (Shortle, 1983 [supra]). Threesense primers (nucS1, nucS2 and nucS3, shown in FIG. 3) were designed,each one having a blunt-ended restriction endonuclease cleavage site forEcoRV or SmaI in a different reading frame with respect to the nuc.gene. Additionally BglII and BamHI were incorporated at the 5′ ends ofthe sense and anti-sense primers respectively to facilitate cloning intoBamHI and BglII cut pTREP1. The sequences of all the primers are givenin FIG. 3. Three nuc gene DNA fragments encoding the mature form of thenuclease gene (NucA) were amplified by PCR using each of the senseprimers combined with the anti-sense primer. The nuc gene fragments wereamplified by PCR using S. aureus genomic DNA template, Vent DNAPolymerase (NEB) and the conditions recommended by the manufacturer. Aninitial denaturation step at 93° C. for 2 min was followed by 30 cyclesof denaturation at 93° C. for 45 sec, annealing at 50° C. for 45seconds, and extension at 73° C. for 1 minute and then a final 5 minextension step at 73° C. The PCR amplified products were purified usinga Wizard clean up column (Promega) to remove unincorporated nucleotidesand primers.

[0123] (c) Construction of the pTREP1-nuc vectors

[0124] The purified nuc gene fragments described in section b weredigested with Bgl II and BamHI using standard conditions and ligated toBamHI and BglII cut and dephosphorylated pTREP1 to generate thepTREP1-nuc1, pTREP1-nuc2 and pTREP1-nuc3 series of reporter vectors.These vectors are described in FIG. 4. General molecular biologytechniques were carried out using the reagents and buffers supplied bythe manufacturer or using standard techniques (Sambrook and Maniatis,Molecular cloning: A laboratory manual. Cold Spring Harbor LaboratoryPress: Cold Spring Harbour (1989)). In each of the pTREP1-nuc vectorsthe expression cassette comprises a transcription terminator,lactococcal promoter P1, unique cloning sites (Bgl II, EcoRV or SmaI)followed by the mature form of the nuc gene and a second transcriptionterminator. Note that the sequences required for translation andsecretion of the nuc gene were deliberately excluded in thisconstruction. Such elements can only be provided by appropriatelydigested foreign DNA fragments (representing the target bacterium) whichcan be cloned into the unique restriction sites present immediatelyupstream of the nuc gene.

[0125] (d) Screening for secreted proteins in Group B Streptococcus.

[0126] Genomic DNA isolated from Group B Streptococcus (S. agalactiae)was digested with the restriction enzyme Tru9I. This enzyme whichrecognises the sequence 5′-TTAA -3′ was used because it cuts A/T richgenomes efficiently and can generate random genomic DNA fragments withinthe preferred size range (usually averaging 0.5-1.0 kb). This size rangewas preferred because there is an increased probability that the P1promoter can be utilised to transcribe a novel gene sequence. However,the P1 promoter may not be necessary in all cases as it is possible thatmany Streptococcal promoters are recognised in L. lactis. DNA fragmentsof different size ranges were purified from partial Tru9I digests of S.agalactiae genomic DNA. As the Tru 9I restriction enzyme generatesstaggered ends the DNA fragments had to be made blunt ended beforeligation to the EcoRV or SmaI cut pTREP1-nuc vectors. This was achievedby the partial fill-in enzyme reaction using the 5′-3′ polymeraseactivity of Klenow enzyme. Briefly Tru9I digested DNA was dissolved in asolution (usually between 10-20 μl in total) supplemented with T4 DNAligase buffer (New England Biolabs; NEB) (1×) and 33 μM of each of therequired dNTPs, in this case dATP and dTTP. Klenow enzyme was added (1unit Klenow enzyme (NEB) per μg of DNA) and the reaction incubated at25° C. for 15 minutes. The reaction was stopped by incubating the mix at75° C. for 20 minutes. EcoRV or SmaI digested pTREP-nuc plasmid DNA wasthen added (usually between 200-400 ng). The mix was then supplementedwith 400 units of T4 DNA ligase (NEB) and T4 DNA ligase buffer (1×) andincubated overnight at 16° C. The ligation mix was precipitated directlyin 100% Ethanol and {fraction (1/10)} volume of 3M sodium acetate (pH5.2) and used to transform L. lactis MG1363 (Gasson, J. Bacteriol.154:1-9 (1983)). Alternatively, the gene cloning site of the pTREP-nucvectors also contains a BglII site which can be used to clone forexample Sau3AI digested genomic DNA fragments.

[0127]L. lactis transformant colonies were grown on brain heart infusionagar and nuclease secreting (Nuc⁺) clones were detected by a toluidineblue-DNA-agar overlay (0.05 M Tris pH 9.0, 10 g of agar per litre, 10 gof NaCl per liter, 0.1 mM CaCl2, 0.03% wt/vol. salmon sperm DNA and 90mg of Toluidine blue O dye) essentially as described by Shortle, 1983[supra], and Le Loir et al., 1994 [supra]). The plates were thenincubated at 37° C. for up to 2 hours. Nuclease secreting clones developan easily identifiable pink halo. Plasmid DNA was isolated from Nuc⁺recombinant L. lactis clones and DNA inserts were sequenced on onestrand using the NucSeq sequencing primer described in FIG. 3, whichsequences directly through the DNA insert.

EXAMPLE 2

[0128] Preparation of a S. agalactiae Standard Inoculum

[0129] Strain validation

[0130]S. agalactiae serotype III (strain 97/0099) is a recent clinicalisolate derived from the cerebral spinal fluid of a new born babysuffering from meningitis. This haemolytic strain of Group BStreptococcus was epidemiologically tested and validated at theRespiratory and Systemic Infection Laboratory, PHLS Central PublicHealth Laboratory, 61 Colindale Avenue, London NW9 5HT. The strain wassubcultured only twice prior to its arrival in the laboratory. Upon itsarrival on an agar slope, a sweep of 4-5 colonies was immediately usedto inoculate a Todd Hewitt/5% horse blood broth which was incubatedovernight statically at 37° C. 0.5 ml aliquots of this overnight culturewere then used to make 20% glycerol stocks of the bacterium forlong-term storage at −70° C. Glycerol stocks were streaked on ToddHewitt/5% horse blood agar plates to confirm viability.

[0131] In Vivo passaging of Group B Streptoccocus

[0132] A frozen culture (described under strain validation) of S.agalactiae serotype III (strain 97/0099) was streaked to single colonieson Todd-Hewitt/5% blood agar plates, which were incubated overnight at37° C. A sweep of 4-5 colonies was used to inoculate a Todd Hewitt/5%horse blood broth, which was again incubated overnight. A 0.5 ml aliquotfrom this overnight culture was used to inoculate a 50 ml Todd Hewittbroth (1:100 dilution) which was incubated at 37° C. 10-fold serialdilutions of the overnight culture were made (since virulence of thisstrain was unknown) and each was passaged intra-peritoneally (IP) inCBA/ca mice in duplicate. Viable counts were performed on the variousinocula used in the passage. Groups of mice were challenged with variousconcentrations of the pathogen ranging from 10⁸ to 10⁴ colony formingunits (cfu). Mice that developed symptoms were terminally anaesthetizedand cardiac punctures were performed (Only mice that had been challengedwith the highest doses, i.e. 1×10⁸ cfu, developed symptoms). Theretrieved unclotted blood was used to inoculate directly a 50 ml serumbroth (Todd Hewitt/20% inactivated foetal calf serum). The culture wasconstantly monitored and allowed to grow to late logarithmic phase. Thepresence of blood in the medium interfered with OD_(600 nm) readings asit was being increasingly lysed with increasing growth of the bacterium,hence the requirement to constantly monitor the culture. Upon reachinglate logarithmic phase/early stationary phase, the culture wastransferred to a fresh 50 ml tube in order to exclude dead bacterialcells and remaining blood cells which would have sedimented at thebottom of the tube. 0.5 ml aliquots were then transferred to sterilecryovials, frozen in liquid nitrogen and stored at −70° C. A viablecount was carried out on a single standard inoculum aliquot in order todetermine bacterial numbers. This was determined to be approximately5×10⁸ cfu per ml.

[0133] Intra-peritoneal challenge and virulence testing of Group BStreptococcus standard inoculum

[0134] To determine if the standard inoculum was suitably virulent foruse in a vaccine trial, challenges were carried out using a dose range.Frozen standard inoculum strain aliquots were allowed to thaw at roomtemperature. From viable count data the number of cfu per ml was alreadyknown for the standard inoculum. Initially, serial dilutions of thestandard inoculum were made in Todd Hewitt broth and mice werechallenged intra-peritoneally with doses ranging from 1×10⁸ to 1×10⁴ cfuin a 500 μl volume of Todd Hewitt broth. The survival times of mousegroups injected with different doses of the bacterium were compared. Thestandard inoculum was determined to be suitably virulent and a dose of1×10⁶ cfu was considered close to optimal for further use in vaccinetrials. Further optimisation was carried out by comparing micechallenged with doses ranging between 5×10⁵ and 5×10⁶ cfu. The optimaldose was estimated to be approximately 2.5×10⁶ cfu. This represented a100% lethal dose and was repeatedly consistent with end-points asdetermined by survival times being clustered within a narrow time-range.Throughout all these experiments, challenged mice were constantlymonitored to clarify symptoms, stages of symptom development as well ascalculating survival times.

[0135] Screening Group B Streptococcal LEEP Derived Genes in DNAVaccination Experiments.

[0136] pcDNA3.1+ as a DNA vaccine vector

[0137] The commercially available pcDNA3.1+plasmid (Invitrogen),referred to as

[0138] pcDNA3.1 henceforth, was used as a vector in all DNA immunisationexperiments involving gene targets derived using the LEEP system unlessstated otherwise.

[0139] pcDNA 3.1 is designed for high-level stable and transientexpression in mammalian cells and has been used widely and successfullyas a host vector to test candidate genes from a variety of pathogens inDNA vaccination experiments (Zhang et al., Infection and Immunity 176:1035-40 (1997); Kurar and Splitter, Vaccine 15: 1851-57 (1997); Andersonet al., Infection and Immunity 64: 3168-3173 (1996)).

[0140] The vector possesses a multiple cloning site which facilitatesthe cloning of multiple gene targets downstream of the humancytomegalovirus (CMV) immediate-early promoter/enhancer which permitsefficient, high-level expression of the target gene in a wide variety ofmammalian cells and cell types including both muscle and immune cells.This is important for optimal immune response as it remains unknown asto which cells types are most important in generating a protectiveresponse in vivo. The plasmid also contains the ColE1 origin ofreplication which allows convenient high-copy number replication andgrowth in E. coli and the ampicillin resistance gene (B-lactamase) forselection in E. coli. In addition pcDNA 3.1 possesses a T7promoter/priming site upstream of the MCS which allows for in vitrotranscription of a cloned gene in the sense orientation.

[0141] Preparation of DNA vaccines

[0142] Oligonucleotide primers were designed for each individual gene ofinterest derived using the LEEP system unless stated otherwise. Eachgene was examined thoroughly, and where possible, primers were designedsuch that they targeted that portion of the gene believed to encode onlythe mature portion of the protein (APPENDIX I); the intention being toexpress those sequences that encode only the mature portion of a targetgene protein to would facilitate its correct folding when expressed inmammalian cells. For example, in the majority of cases primers weredesigned such that putative N-terminal signal peptide sequences wouldnot be included in the final amplification product to be cloned into thepcDNA3. 1 expression vector. The signal peptide directs the polypeptideprecursor to the cell membrane via the protein export pathway where itis normally cleaved off by signal peptidase I (or signal peptidase II ifa lipoprotein). Hence the signal peptide does not make up any part ofthe mature protein whether it be displayed on the bacterium's surface orsecreted. Where an N-terminal leader peptide sequence was notimmediately obvious, primers were designed to target the whole of thegene sequence for cloning and ultimately, expression in pcDNA3.1.

[0143] All forward and reverse oligonucleotide primers incorporatedappropriate restriction enzyme sites to facilitate cloning into thepcDNA3.1 MCS region. All forward primers were also designed to includethe conserved Kozak nucleotide sequence 5′-gccacc-3′ immediatelyupstream of an ‘atg’ translation initiation codon in frame with thetarget gene insert. The Kozak sequence facilitates the recognition ofinitiator sequences by eukaryotic ribosomes. Typically, a forward primerincorporating a BamH1 restriction enzyme site the primer would beginwith the sequence 5′-cggatccgccaccatg-3′, followed by a sequencehomologous to the 5′ end of that part of a gene being amplified. Allreverse primers incorporated a Not I restriction enzyme site sequence5′-ttgcggccgc-3′. All gene-specific forward and reverse primers weredesigned with compatible melting temperatures to facilitate theiramplification.

[0144] All gene targets were amplified by PCR from S. agalactiae genomicDNA template using Vent DNA polymerase (NEB) or rTth DNA polymerase (PEApplied Biosystems) using conditions recommended by the manufacturer. Atypical amplification reaction involved an initial denaturation step at95° C. for 2 minutes followed by 35 cycles of denaturation at 95° C. for30 seconds, annealing at the appropriate melting temperature for 30seconds, and extension at 72° C. for 1 minute (1 minute per kilobase ofDNA being amplified). This was followed by a final extension period at72° C. for 10 minutes. All PCR amplified products were extracted oncewith phenol chloroform (2:1:1) and once with chloroform (1:1) andethanol precipitated. Specific DNA fragments were isolated from agarosegels using the QIAquick Gel Extraction Kit (Qiagen). The purifiedamplification gene DNA fragments were digested with the appropriaterestriction enzymes and cloned into the pcDNA3.1 plasmid vector using E.coli as a host. Successful cloning and maintenance of genes wasconfirmed by restriction mapping and by DNA sequencing. Recombinantplasmid DNA was isolated on a large scale (>1.5 mg) using Plasmid MegaKits (Qiagen).

[0145] DNA vaccination trials

[0146] DNA vaccine trials in mice were accomplished by theadministration of DNA to 6 week old CBA/ca mice (Harlan, UK). Mice to bevaccinated were divided into groups of six and each group was immunisedwith recombinant pcDNA3.1 plasmid DNA containing a specific target-genesequence derived using the LEEP system unless stated otherwise. A totalof 100 μg of DNA in Dulbecco's PBS (Sigma) was injected intramuscularlyinto the tibialis anterior muscle of both hind legs. Four weeks laterthis procedure was repeated using the same amount of DNA. Forcomparison, control mice groups were included in all vaccine trials.These control groups were either not DNA-vaccinated or were immunisedwith non-recombinant pcDNA3.1 plasmid DNA only, using the same timecourse described above. Four weeks after the second immunisation, allmice groups were challenged intra-peritoneally with a lethal dose of S.agalactiae serotype III (strain 97/0099). The actual number of bacteriaadministered was determined by plating serial dilutions of the inoculumon Todd-Hewitt/5% blood agar plates. All mice were killed 3 or 4 daysafter infection. During the infection process, challenged mice weremonitored for the development of symptoms associated with the onset ofS. agalactiae induced-disease. Typical symptoms in an appropriate orderincluded piloerection, an increasingly hunched posture, discharge fromeyes, increased lethargy and reluctance to move which was often theresult of apparent paralysis in the lower body/hind leg region. Thelatter symptoms usually coincided with the development of a moribundstate at which stage the mice were culled to prevent further suffering.These mice were deemed to be very close to death, and the time ofculling was used to determine a survival time for statistical analysis.Where mice were found dead, a survival time was calculated by averagingthe time when a particular mouse was last observed alive and the timewhen found dead, in order to determine a more accurate time of death.The results of this trial are shown in Table land presented graphicallyin FIG. 2.

[0147] Interpretation of Results

[0148] A positive result was taken as any DNA sequence that was clonedand used in challenge experiments as described above and gave protectionagainst that challenge. DNA sequences were determined to be protective;

[0149] if that DNA sequence gave statistically significant protection tomice as compared to control mice (to a 95% confidence level (p>0.05) asdetermined using the Mann-Whitney U test.

[0150] if that DNA sequence was marginal or non-signficant usingMann-Whitney but showed some protective features. For example, one ormore outlying mice may survive for significantly longer time periodswhen compared with control mice. Alternatively, the time to first deathmay also be prolonged when compared to counterpart mice in controlgroups. It is acceptable to allow marginal or non-significant results tobe considered as potential positives when it is possible that theclarity of some results may be affected by problems associated with theadministration of the DNA vaccine. Indeed, much varied survival timesmay reflect different levels of immune response between differentmembers of a given group. TABLE 1 LEEP DNA immunisation and GBSchallenge Experiment Statistical analysis of survival times MeanSurvival Times (hours) UnVacc 3-60(ID-65) 3-5(ID-66) 1 27.583 54.41642.916 2 27.583 31.000 42.916 3 24.583 43.000 32.874 4 22.250 34.91642.916 5 35.916 38.958 27.333 6 22.250 34.916 30.916 Mean 27.583 40.45837.791 sd 5.1691 8.9959 7.2860 p value 0.0098 0.0215

[0151] Comment

[0152] ID-65 (3-60)

[0153] Mice immunised with the ‘3-60 (ID-65)’ DNA vaccine exhibitedsignificantly longer survival times when compared with the unvaccinatedcontrol group.

[0154] ID-66 (3-5)

[0155] Mice immunised with the ‘3-5 (ID-66)’ DNA vaccine exhibitedsignificantly longer survival times when compared with the unvaccinatedcontrol group.

EXAMPLE 3

[0156] Expression and Screening Group B Streptococcal LEEP DerivedProteins in Protein Vaccination Experiments.

[0157] Expression of proteins

[0158] Prioritised genes ie, those selected on the basis of predictedexpression features as deduced from sequence characteristics (asdescribed in FIG. 1), were cloned and expressed as recombinant proteinsusing the pET system (Novagen, Inc., Madison, Wis.) utilisingEscherichia coli as a host. Target genes were cloned into the pET28b(+)plasmid expression vector. The pET28b(+) vector is designed for highlevel expression and purification of target proteins. This vectorcarries a T7 promoter for transcription of a target gene, followed by anN-terminal HisTag®/thrombin/T7Tag® configuration, a multi-cloning sitecontaining unique restriction enzyme sites for cloning purposes, and anoptional C-terminal HisTag sequence. The vector also carries akanamycin resistance gene for selection purposes and for maintainingtarget gene expression (pET System Manual, 8^(th) edition, Novagen).

[0159] Preparation of protein vaccines

[0160] Oligonucleotide primers were designed for each individual targetgene derived using the LEEP system unless stated otherwise. Each genewas examined thoroughly. Where possible primers were designed so thatthey would target that part of the gene predicted to encode only themature portion of the protein (APPENDIX II). It is hoped that expressingthose corresponding to the predicted mature protein only, mightfacilitate its correct folding when finally expressed in vitro.Oligonucleotide primers were designed so that sequences, encoding theputative N-terminal signal peptide of the target protein, would not beincluded in the final amplification product to be cloned pET28b(+). Thesignal peptide directs the polypeptide precursor to the cell membranevia the protein export pathway where it is normally cleaved off bysignal peptidase I (or signal peptidase II if a lipoprotein). Hence thesignal peptide would not be expected to form any part of the maturetarget protein, whether it be displayed on the bacterium's surface orsecreted. For this purpose, classical signal peptides and their cleavagesites were predicted using the DNA Strider™ Program (CEA, France) andthe SignalP V1.1 program, which predicts the presence and location ofsignal peptide cleavage sites in amino acid sequences from differentorganisms (Nielsen et al., Protein Engineering 10: 1-6 (1997)). Where aN-terminal leader peptide sequence was not obvious, primers weredesigned to include the whole of the gene sequence for cloning andexpression.

[0161] All oligonucleotide primers were designed to incorporateappropriate restriction enzyme sites to facilitate cloning into thepcDNA3.1 MCS region (APPENDIX II). Forward primers included an Nco I(5′-ccatgg-3′) or Nhe I (5′-gctagc-3′) restriction enzyme site and an‘ATG’ start codon in-frame with the target gene open reading frame(orf). All reverse primers included a Not I restriction enzyme site5′-gcggccgc-3′ and were designed so that the target gene could beexpressed in frame with the C-terminal HisTag (i.e. the stop codon ofthe target gene was not included). Using the Nco I and Not I, allowedthe removal of the N-terminal HisTag®, thrombin and T7Tag® DNAsequences. At the same time target genes were cloned immediatelydownstream of a highly efficient ribosome binding site (from the phageT7 major capsid protein), to facilitate high levelexpression/translation of the target gene by T7 RNA polymerase, andsubsequent purification by means of the C-terminal HisTag. All targetgene-specific forward and reverse primers were designed with compatiblemelting temperatures to facilitate their amplification.

[0162] All gene targets were amplified by PCR from S. agalactiae genomicDNA template using Vent DNA polymerase (NEB) using conditionsrecommended by the manufacturer. A typical amplification reactioninvolved an initial denaturation step at 95° C. for 2 minutes followedby 35 cycles of denaturation at 95° C. for 30 seconds, annealing at theappropriate melting temperature for 30 seconds, and extension at 72° C.for 1 minute (1 minute per kilobase of DNA being amplified). This wasfollowed by a final extension period at 72° C. for 10 minutes. All PCRamplified products were extracted .once with phenol:chloroform (2:1:1)and once with chloroform (1:1) and ethanol precipitated. Specific DNAfragments were isolated from agarose gels using the QIAquick GelExtraction Kit (Qiagen). Purified target gene DNA amplicons were thendigested Nco I (or Nhe I) and Not I restriction enzymes, and cloned intoNco I and Not I digested pET28b(+) plasmid vector using E. coli DH5α orE. coli BL21 (DE3) as a host. Successful cloning and maintenance ofgenes was confirmed by restriction mapping.

[0163] Deternination of target protein expression and solubility

[0164] Glycerol stocks of E. coli BL21 DE3 pET28b(+) strains expressingrecombinant proteins were used to inoculate 10 ml Luria broth containingKanamycin (30 μg/ml) which were grown overnight at 37° C. with vigorousshaking (300 rpm).

[0165] A 20-40 ml Luria broth containing Kanarnycin (30 μg/ml) wasinoculated with 1:100 dilution of the overnight culture from step 1 andgrown at 37° C. with vigorous shaking (300 rpm). When the culturereached an OD₆₀₀ of between 0.6 and 1.0, IPTG was added to a finalconcentration of 1 mM. Typically cultures were induced for 3 hours.Cells were then harvested by centrifugation at 7000 g for 10 min. Thecell pellet was then resuspended in {fraction (1/10)} volume of lysisbuffer (50 mM NaH₂PO₄, pH.8.0; 300 mM NaCl;10 mM imidazole; 10%glycerol). Lysozyme was then added to a final concentration of 1 mg/ml,and the suspension was incubated on ice for 30 min. The suspension wasthen sonicated on ice (six 10-sec bursts at 200-300 W with a 10-seccooling period. The lysate was then centrifuged at 10,000g for 20 min.The supernatant (containing soluble protein) was transferred to asterile 2 ml eppendorf. The pellet was resuspended in 2 ml ofsolubilisation buffer (8 M Urea; 50 mM NaH₂PO₄, pH.8.0; 300 mM NaCl; 10%glycerol). This suspension contained the insoluble protein fraction.Aliquots from both the soluble and insoluble fractions were transferredto new eppendorfs. The protein samples were denatured by adding an equalvolume of 2×SDS-PAGE buffer and heating at 95° C. for 5 min. Denaturedextract samples were then analysed by SDS-PAGE to determine target geneexpression and solubility.

[0166] Large scale expression of recombinant target proteins

[0167] Glycerol stocks of E. coli BL21 DE3 pet28b(+) strains expressingrecombinant proteins were used to inoculate 10 ml Luria broth containingKanamycin ( 30 μg/ml) which were grown overnight at 37° C. with vigorousshaking (300 rpm). 5 ml of an overnight culture of a recombinant strainwas used to inoculate a 250 ml Luria broth containing kanamycin (30μg/ml) which was grown at 37° C. with vigorous shaking (300 rpm). Whenthe culture reached an OD₆₀₀ of between 0.6 and 1.0, IPTG was added to afinal concentration of 1 mM. Typically, cultures were induced for 3hours. Cultures were then centrifuged to a pellet and stored frozen at−20° C.

[0168] Purification of target antigens.

[0169] Ni-NTA agarose (Qiagen LTD, West Sussex, UK; Cat. No. 30210) wasused to purify the His-Tagged recombinant proteins. The 6×His affinitytag which was expressed in frame with the target proteins in pET28b(+),facilitates binding to Ni-NTA. Ni-NTA offers high binding capacity (withminimal non-specific binding) and can bind 5-10 mg of 6×His-taggedprotein per ml of resin. The 6×His-tag is poorly immunogenic, and at pH8.0, the tag is small, uncharged and therefore does not generallyinterfere with the structure and function of the protein (TheQIAexpressionist, Qiagen Handbook, March 1999).

[0170] NOTE: All the proteins (LEEP-derived, unless stated otherwise)described here were purified under denaturing conditions except ID-65.ID-65 was prepared and purified under native conditions.

[0171] Purification under native conditions

[0172] The frozen pellet was allowed to thaw on ice for 15 minutes andthen resuspended in 10 ml of lysis buffer (50 mM NaH₂PO₄, pH.8.0; 300 mMNaCl;10 mM imidazole; 10% glycerol). Lysozyme was then added to a finalconcentration of 1 mg/ml, and the suspension was incubated on ice for 30min. The suspension was then sonicated on ice (six 10-sec bursts at200-300 W with a 10-sec cooling period0. Dnase I (5 μg/ml) was thenadded to the lysate, which was then incubated on ice for 10-15 min. Thelysate was then centrifuged at 10,000 rpm for 20 min at 4° C. to pelletcell debris. The clear lysate supernatant was then loaded into apolypropylene column (Qiagen; Cat. No. 34964), bottom cap attached. 1.5ml of 50% Ni-NTA was then added, the column sealed and the suspensionwas allowed to mix gently using a rotating wheel for 1-2 hours at 4° C.The column containing the lysate/Ni-NTA mix was then placed uprightusing a retort stand, and the Ni-NTA was allowed to settle. The bottomcap was removed and the lysate was allowed to flow through. The columnwas then washed with three to six 4 ml volumes of wash buffer (50 mMNaH₂PO₄, pH.8.0; 300 mM NaCl;20 mM imidazole; 10% glycerol). The proteinwas then eluted in 0.5 ml aliquots of elution buffer (500 mM NaH₂PO₄,pH.8.0; 300 mM NaCl;500 mM imidazole; 10% glycerol). Eluate fractionswere then analysed by SDS-PAGE and those containing the protein werepooled and dialysed against a PBS (pH 7.0)-glycerol (10%) solution.

[0173] Purification and refolding under denaturing conditions

[0174] The frozen pellet was allowed to thaw on ice for 15 minutes andthen resuspended in 10 ml of buffer containing 8 M Urea, 300 mM NaCl,10% glycerol, 0.1 M NaH₂PO₄, pH.8.0, and 10 mM imidazole. The cells werethen lysed by gentle vortexing for 1 hour at room temperature. Thelysate was then centrifuged at 10,000 g for 20 minutes to pelletcellular debris. The clear lysate supernatant was then loaded into apolypropylene column (Qiagen; Cat. No. 34964), bottom cap attached. 1.5ml of 50% Ni-NTA slurry was then added, the column sealed and thesuspension was allowed to mix gently using a rotating wheel for 1-2hours at room temperature. The column containing the lysate/Ni-NTA mixwas then placed upright using a retort stand, and the Ni-NTA was allowedto settle. The bottom cap was removed and the lysate was allowed to flowthrough. The column was then washed with 4-8 ml of buffer containing 8 MUrea, 300 mM NaCl, 10% glycerol, 0.1 M NaH₂PO₄, pH 8.0, and 10 mMimidazole. The resin was then washed with a gradient of 6 to 0 M in abuffer containing 0.1 M NaH₂PO₄, pH.8.0, 300 mM NaCl and 10% glycerol tofacilitate the slow removal of urea and gradual refolding of targetprotein. The resin was then washed with a buffer containing 0.1 MNaH₂PO₄, pH 7.0, 500 mM NaCl and 10% glycerol. The recombinant proteinwas then eluted in 0.5 ml aliquots with 500 mM Imidazole in 0.1 mMNaH₂PO₄, pH 7.0, 500 mM NaCl and 10% glycerol. The fractions wereanalysed on SDS-PAGE and those containing the protein were pooled anddialysed against a PBS (pH 7.0)-glycerol (10%) solution.

[0175] All purified proteins were analysed by SDS-PAGE, as shown inFIGS. 5, 6 and 7, prior to their use as antigens in immunisation andvaccination experiments.

[0176] Protein Vaccinations

[0177] Vaccines were composed of the target protein in phosphatebuffered saline/10% glycerol and mixed with aluminium hydroxide (alum)(Imject® Alum, Pierce, Rockford, Ill.). Each dose (unless otherwisestated) of vaccine contained 25 μg of purified protein in 50 μl ofPBS/10% glycerol, mixed with 50 μl of alum. Groups of 6-8 CBA/ca mice(Harlan, UK) were immunised subcutaneously with the vaccines and again 4weeks later. A control group received 100 μl dose of PBS/10% glycerolwith alum. All vaccinated groups consisted of 6 mice. Mice werechallenged at 7 weeks (unless otherwise stated). Mice were injectedintraperitoneally (i.p.) with between 2.5-5×10⁶ bacteria diluted in 0.5ml Todd-Hewitt broth. Deaths were recorded daily for 7 days. Thechallenged mice were observed daily for signs of illness. Typicalsymptoms in an appropriate order included piloerection, an increasinglyhunched posture, discharge from eyes, increased lethargy and reluctanceto move which was often the result of apparent paralysis in the lowerbody/hind leg region. The latter symptoms usually coincided with thedevelopment of a moribund state at which stage the mice were culled toprevent further suffering. These mice were deemed to be very close todeath, and the time of culling was used to determine a survival time forstatistical analysis. Where mice were found dead, a survival time wascalculated by averaging the time when a particular mouse was lastobserved alive and the time when found dead, in order to determine amore accurate time of death.

[0178] Analysis of antibody responses

[0179] Mice (6 per group) were immunised with two doses of vaccine witha four week interval. Mice were tail bled at 3 weeks and 6 weeks postprimary vaccination to obtain sera. Total Immunoglobulin G (IgG) titresto the vaccine protein component in sera were determined byenzyme-linked immunosorbent assay (ELISA), using the original purifiedprotein as the coating antigen.

[0180] Standard ELISA protocol

[0181] Solutions

[0182] Carbonate/bicarbonate buffer, pH 9.8

[0183] 0.80 g Na₂CO₃

[0184] 1.46 g NaHCO₃

[0185] pH to 9.6 using HCl

[0186] Add distilled water (dH₂O) to a final volume of 500 ml.

[0187] n-NITROPHENYL PHOSPHATE SUBSTRATE

[0188] Diethanolamine Buffer, pH 9.8

[0189] 48.5 ml diethanolamine

[0190] pH to 9.8 using 1M HCl

[0191] Add dH₂O to a final volume of 500 ml

[0192] NOTE: ELISAs were optimised for each protein submitted forimmunisation.

[0193] Protocol

[0194] 1. ELISA plates (Greiner labortechnik 96 well plates: Cat. No.655061) with an appropriate concentration of recombinant protein dilutedin carbonate/bicarbonate buffer (50 μl/well). Cover plates with plasticor foil and leave overnight at 4° C.

[0195] 2. Quickly wash plates twice in a tub/container containingPBS/0.05%Tween-20 and then pat dry.

[0196] 3. Block plates with 3% BSA in PBS/Tween (100 μl/well) for 1 hourat room temperature.

[0197] 4. Wash the plates 3 times PBS/Tween as before and pat dry asbefore.

[0198] 5. Apply (primary antibody) protein-specific antiserum(50μl/well) diluted from {fraction (1/50)} in a doubling dilution seriesin PBS/Tween and incubate at room temperature for 90 minutes.

[0199] 6. Wash plates as before (3 times quickly), followed up by 2×3minute soaks (in PBS/Tween)

[0200] 7. Apply diluted secondary antibody alkaline phosphataseconjugate. For anti-mouse Total IgG alkaline phospatase conjugate (GoatAnti-Mouse IgG-AP, Southern Biotechnology Associates, Birmingham, Ala.Cat. No. 1030-04) dilute {fraction (1/3000)} in PBS/Tween and apply 50μl per well and incubate at room temperature for 90 minutes.

[0201] 8. Wash plates as in step 6.

[0202] 9. Apply substrate. Dissolve one 5 mg tablet of nitrophenylphosphate (Sigma: kept in freezer) in 5 ml of diethanolamine buffer.Apply 100 μl per well. Cover with foil (a light-sensitive reaction) andleave at room temperature for 30 minutes. Read Optical densities (OD) ata wavelength of 405 nm.

[0203] 10. Plot curves of OD Vs dilution (log scale). Calculateend-point titres as the dilution giving the same OD as the mean of theOD obtained from the wells containing the {fraction (1/50)} dilution ofpre-immune serum.

[0204] ELISA Plate format 2° 1/50 1/100 1/200 1/400 1/800 1/1600 1/32001/6400 1/12800 1/25600 1/51200 1° Duplicate Pre Pre Pre

[0205] The dilution series used is indicated (see first row of table).Beginning with a {fraction (1/50)} dilution, sera are diluted two-foldin PBS/Tween in doubling dilution series as indicated.

[0206] Protein Immunisation data

[0207] ID-65 and ID-83

[0208] The ID-65 and ID-83 vaccines were composed of the target proteinsin phosphate buffered saline/10% glycerol mixed with aluminium hydroxide(alum) (Imject®Alum, Pierce, Rockford, Ill.). Each dose of vaccinecontained 20 μg of purified protein in 100 μl of PBS/10% glycerol, mixedwith 50 μl of alum. A group of 6-8 week old CBA/ca mice (Harlan, UK)were immunised subcutaneously with the ID65 and ID-83 vaccine and again4 weeks later. A control group received a 150 μl dose of PBS/10%glycerol (2:1) with alum. All groups consisted of 6 mice. Mice were tailbled at 5 weeks post primary vaccination to obtain sera. The presence oftotal Immunoglobulin G (IgG) antibodies to the ID-65 and ID-83 proteinin sera was determined by enzyme-linked immunosorbent assay (ELISA),using the purified protein as the coating antigen. ELISA was alsoperformed using sera obtained at 6 weeks post-primary vaccination fromthe PBS/10% glycerol immunised control group.

[0209] NOTE: ELISA plates were coated with the ID-65 or ID-83 proteinsat a concentration of 1 μg/ml.

[0210] Protein Vaccination—ELISA Results for ID-65 and ID-83

[0211] Mice (6 per group) were immunised with two doses of the ID-65 andID-83 vaccines with a four week interval. Mice were tail bled at 5 weekspost primary vaccination to obtain sera. The Immunoglobulin G (IgG)titres to the vaccine protein component in sera were determined byenzyme-linked immunosorbent assay (ELISA), using the purified ID-65 andID-83 proteins as the coating antigen. Subsequent to optimisation, ELISAplates were coated at a concentration 1 ug/ml for both the purified ID65and ID-93 proteins. Total IgG titres were measured against pre-immuneserum ({fraction (1/50)} dilution). The results are shown in Table 2 andgraphically in FIG. 8. TABLE 2 Serum (Group) ID-65 + Alum PBS + AlumID-83 + Alum PBS + Alum (n = 6) (n = 6) (n = 6) (n = 6) Coating antigenID-65 ID-83 Bleed 5 weeks 5 weeks 5 weeks 5 weeks Total IgG 7535763 96582081 61 Titres 1557649 90 50027 50 (mouse 3319737 108 154670 80 1-6)1832259 176 57901 96 8794360 371 66497 125 1445728 0 49928 0 Average4080916 285 76851 69 Standard 3258818 355 39985 43 Deviation

[0212] Protein Immunisation and Challenge data (ID-93)

[0213] ID-93

[0214] The ID-93 vaccine was composed of the target ID-93 protein inphosphate buffered saline/10% glycerol mixed with aluminium hydroxide(alum) (Imject®Alum, Pierce, Rockford, Ill.). Each dose of vaccinecontained 25 μg of purified protein in 100 μl of PBS/10% glycerol, mixedwith 100 μl of alum. A group of 6-8 week old CBA/ca mice (Harlan, UK)were immunised subcutaneously with the ID-93 vaccine and again 4 weekslater. A control group received PBS/10% glycerol with alum. Both groupsconsisted of 6 mice. Mice were challenged at 7 weeks (unless otherwisestated). Mice were injected intraperitoneally (i.p.) with 5×10⁶ bacteriadiluted in 0.5 ml Todd-Hewitt broth. The challenged mice were observeddaily for signs of illness. Deaths were recorded daily for 7 days.Survival data are shown in Table 3 and graphically in FIG. 9.

[0215] Mice were tail bled at 3 weeks and 6 weeks post primaryvaccination to obtain sera. The presence of total Immunoglobulin G (IgG)antibodies to the ID-93 protein in sera was determined by enzyme-linkedimmunosorbent assay (ELISA), using the pure ID-93 protein as the coatingantigen. ELISA was also performed using sera obtained at 6 weekspost-primary vaccination from the PBS/10% glycerol immunised controlgroup.

[0216] Note: ELISA plates were coated with the ID-93 protein at aconcentration of 1 μg/ml. TABLE 3 ID-93 protein immunisation and GBSchallenge experiment Statistical analysis of Survival Times Group PBS +Alum ID-93 + Alum Survival 22.37 29.37 Times 22.37 35.12 (hours) 15.3732.62 28.03 32.62 29.53 37.12 26.53 27.87 Mean 24.03 32.45 sd 5.16 3.45p value 0.01

[0217] Comment

[0218] ID-93 (RS-70)

[0219] Mice immunised with the ID-93-Alum vaccine exhibitedsignificantly longer survival times when compared with the PBS-Alumcontrol group.

[0220] (Statistical Significance was determined by the Mann-Whitney Utest using a 95% confidence level (p>0.05).

[0221] Protein Vaccination—ELISA results for ID-93

[0222] Mice (6 per group) were immunised with two doses of the ID-93vaccine with a four week interval. Mice were tail bled at 3 weeks and 6weeks post primary vaccination to obtain sera. The Immunoglobulin G(IgG) titres to the vaccine protein component in sera were determined byenzyme-linked immunosorbent assay (ELISA), using the purified ID-93protein as the coating antigen. Subsequent to optimisation, ELISA plateswere coated with the purified ID-93 protein at a concentration of 1μg/ml. Total IgG titres were measured against pre-immune serum({fraction (1/50)} dilution). The results are shown in Table 4 andgraphically in FIG. 10. TABLE 4 Serum Group PBS/10% glycerol ID-93 +Alum(n = 6) (n = 6) (control) Coating antigen ID-93 ID-93 ID-93 ID-93Bleed 3 weeks 6 weeks 3 weeks 6 weeks Total IgG 87196 3000000 39 100Titres 99544 8000000 31 16 (mouse 1-6) 19620 2000000 31 79 3472410000000 59 48 59990 10000000 24 328 30041 4000000 13 40 Average 551866166667 33 102 Standard 32654 3600926 15 115 error

[0223] Protein Immunisation Data

[0224] ID-89 and ID-96

[0225] The ID-89 and ID-96 vaccines were composed of the target proteinsin phosphate buffered saline/10% glycerol mixed with TitreMax Goldadjuvant (Sigma, Mo., USA) according to the manufacturers instructions.The ID-89 vaccine contained 25 μg of purified protein 50 μl of PBS/10%glycerol, mixed with 50 μl of TitreMax Gold. The ID-96 vaccine contained12.5 μg of purified protein 50 μl of PBS/10% glycerol, mixed with 50 μlof TitreMax Gold. Groups of 6-8 week old CBA/ca mice (Harlan, UK) wereimmunised subcutaneously with the ID-89 and ID-96 vaccines and again 4weeks later. A control group received a 100 μl dose PBS/10% glycerolwith TitreMax Gold (1:1). Both groups consisted of 6 mice. Mice weretail bled at 3 weeks and 6 weeks post primary vaccination to obtainsera. The presence of total Immunoglobulin G (IgG) antibodies to theID-65 and ID-83 protein in sera was determined by enzyme-linkedimmunosorbent assay (ELISA), using the purified protein as the coatingantigen. ELISA was also performed using sera obtained at 3 weeks and 6weeks post-primary vaccination from the PBS/10% glycerol immunisedcontrol group.

[0226] Note: ELISA plates were coated with the ID-89 or ID-96 proteinsat a concentration of 1 μg/ml and 3 μg/ml respectively.

[0227] Protein Vaccination—ELISA results for ID-89 and ID-96

[0228] Mice (6 per group) were immunised with two doses of the ID-89 andID-96 vaccines with a four week interval. Mice were tail bled at 3 weeksand 6 weeks post primary vaccination to obtain sera. The ImmunoglobulinG (IgG) titres to the vaccine protein component in sera were determinedby enzyme-linked immunosorbent assay (ELISA), using the purified ID-65and ID-83 proteins as the coating antigen. Subsequent to optimisation,ELISA plates were coated with purified ID-89 and ID-96 protein at aconcentration 1 ug/ml and 3 μg/ml respectively. Total IgG titres weremeasured against pre-immune serum ({fraction (1/50)} dilution). ELISAwas also performed on both proteins using sera obtained at 3 weeks and 6weeks post-primary vaccination from the PBS/10% glycerol immunisedcontrol group. Results are shown in tables 5 a and 5 b and graphicallyin FIG. 11. TABLE 5a Serum ID-89 + TitreMax Gold ID-96 + TitreMax Gold(n = 6) (n = 6) Coating ID-89 ID-96 antigen Bleed 3 weeks 6 weeks 3weeks 6 weeks Total IgG 146940 1000000 190371 10000000 Titres 896721000000 212505 10000000 (mouse 1-6) 173532 2000000 167613 5000000 85161751210 110378 5000000 8895 551281 142614 1000000 27880 2000000 1910851000000 Average 10202 1217082 169094 5333333 Standard 51451 629364 373414033196 Deviation

[0229] TABLE 5B Serum PBS/10% glycerol PBS/10% glycerol (n = 6) (n = 6)Coating ID-89 ID-96 protein Bleed 3 weeks 6 weeks 3 weeks 6 weeks Total3 7 33 31 IgG 8 18 77 62 Titres 29 31 77 1 (mouse 1-6) 34 4 52 29 0 2125 31 5 1 113 0 Average 13 11 80 26 Standard 15 12 35 23 deviation

EXAMPLE 4

[0230] Conservation and variability of candidate vaccine antigen genesamong different isolates of Group B Streptococci

[0231] An initial Southern blot analysis was carried out to determinecross-serotype conservation of novel Group B Streptococcal genesisolated using the LEEP system unless stated otherwise. Analysing theserotype distribution of a target gene will also determine theirpotential use as antigen components in a GBS vaccine. The Group BStreptococcal strains whose DNA was analysed as part of this study arelisted in APPENDIX III

[0232] Amplification and labelling of specific target genes as DNAprobes for southern blot analysis.

[0233] Oligonucleotide primers were designed for each individual gene ofinterest derived using the LEEP system unless stated otherwise. The sameprimers already described in APPENDIX II were used to amplifycorresponding gene-specific DNA probes. Specific gene targets wereamplified by PCR using Vent DNA polymerase (NEB) according to themanufacturers instructions. Typical reactions were carried out in a 100μl volume containing 50 ng of GBS template DNA, a one tenth volume ofenzyme reaction buffer, 1 μM of each primer, 250 μM of each dNTP and 2units of Vent DNA polymerase. A typical reaction contained an initial 2minute denaturation at 95° C., followed by 35 cycles of denaturation at95° C. for 30 seconds, annealing at the appropriate melting temperaturefor 30 seconds, and extension at 72° C. for 1 minute (1 minute perkilobase of DNA being amplified). The annealing temperature wasdetermined by the lower melting temperature of the two oligonucleotideprimers. The reaction was concluded with a final extension period of 10minutes at 72° C.

[0234] All PCR amplified products were extracted once with phenolchloroform (2:1:1) and once with chloroform (1:1) and ethanolprecipitated. Specific DNA fragments were isolated from agarose gelsusing the QIAquick Gel Extraction Kit (Qiagen). For use as DNA probes,purified amplified gene DNA fragments were labelled with digoxygeninusing the DIG Nucleic Acid Labelling Kit (Boehringer Mannheim) accordingto the manufacturer's instructions.

[0235] Southern blot Hybridisation analysis of Group B Streptococcalgenomic DNA

[0236] Genomic DNA had previously been isolated from all strains ofGroup B Streptococci which were investigated for conservation ofLEEP-derived (unless stated otherwise) gene targets. Appropriate DNAconcentrations were digested using either Hin DIII or Eco RI restrictionenzymes (NEB) according to manufacturer instructions and analysed byagarose gel electrophoresis. Following agarose gel electrophoresis ofDNA samples, the gel was denatured in 0.25M HCl for 20 minutes and DNAwas transferred onto Hybond™ N⁺ membrane (Amersham) by overnightcapillary blotting. The method is essentially as described in Sambrooket al. (1989) using Whatman 3MM wicks on a platform over a reservoir of0.4M NaOH. After transfer, the filter was washed briefly in 2×SSC andstored at 4° C. in Saran wrap (Dow chemical company).

[0237] Filters were prehybridised, hybridised with the digoxygeninlabelled DNA probes and washed using conditions recommended byBoehringer Mannheim when using their DIG Nucleic Acid Detection Kit.Filters were prehybridised at 68° C. for one hour in hybridisationbuffer (1% w/v supplied blocking reagent, 5×SSC, 0.1% v/v N-laurylsarcosine, 0.02% v/v sodium dodecyl sulphate[SDS]). The digoxygeninlabelled DNA probe was denatured at 99.9° C. for 10 minutes before beingadded to the hybridisation buffer. Hybridisation was allowed to proceedovernight in a rotating Hybaid tube in a Hybaid Mini-hybridisation oven.Unbound probe was removed by washing the filter twice with 2×SSC-0.1%SDS for 5 minutes at room temperature. For increased stringency filterswere then washed twice with 0.1×SSC-0.1% SDS for 15 minutes at 68° C.The DIG Nucleic Acid Detection Kit (Boehringer Mannheim) was used toimmunologically detect specifically bound digoxygenin labelled DNAprobes.

[0238] Results of Southern blot analysis

[0239] Unless otherwise stated, all genomic digests and theircorresponding Southern blots followed an identical lane order asdescribed in Table 6 below. TABLE 6 Lane 1 2 3 4 5 6 7 Strain 1 kb 515A909 SB35 H36B 18RS21 1954/92 molecular Serotype Weight Ia Ia Ib Ib IIII Marker Lane 8 9 10 11 12 13 14 Strain 118/158 97/0057 BS30 M78197/0099 3139 1169-NT Serotype II II III III III IV V Lane 15 16 17 18 1920 Strain GBS 6 7271 JM9 Group Streptococcus 1 kb A pneumoniae molecularStrepo- coccus Serotype VI VII VIII — 14 Weight Marker

[0240] For comparative purposes, it was decided to analyse the serotypedistribution of the GBS rib gene, which encodes the known protectiveimmunogen Rib. Rib has previously been shown to be present in serotypeIII and some strains of serotype II but not in serotypes Ia or Ib(Stalhammar-Carlemalm et al., J. Exp. Med. 177: 1593-1603 (1993)).

[0241] Confirmation of this pattern would not only give increasedconfidence in interpreting subsequent results, it would also determineif a rib gene homologue was present in the remaining GBS serotypes beinginvestigated here. Primers designed for the amplification of rib for useas a gene probe in Southern blot analysis are described in APPENDIX II.TABLE 7 Lane order for FIG. 12 (rib gene Southern blot analysis) Lane 12 3 4 5 6 7 strain 1 kb 515 A909 SB35 H36B 18RS21 1954/92 molecularserotype Weight Ia Ia Ib Ib II II Marker Lane 8 9 10 11 12 13 14 strain118/158 97/0057 BM110 BS30 M781 97/0099 3139 serotype II II III III IIIIII IV Lane 15 16 17 18 19 20 strain 1169-NT GBS 6 7271 JM9 GroupStreptococcus A pneumoniae Strepo- coccus serotype V VI VII VIII — 14

[0242] Rib (FIG. 12) Comment

[0243] The Southern blot analysis shown in FIG. 12 indicates that therib gene is not conserved across all GBS serotypes. rib appears to beabsent from all serotype Ia and Ib strains (lanes 2 to 5) and fromstrains 118/158 and 97/0057 of serotype II (lanes 8 and 9). However, ribwould appear to present in strains 18RS21 and 1954/92 of serotype II(lanes 6 and 7) and in all strains of serotype III (lanes 10 to 13).This is in agreement with previously published data(Stalhammar-Carlemalm et al., 1993 [supra]). rib would also appear to bepresent in strains representing serotypes VII and VII (lanes 17 and 18)but was absent from strains representing serotypes IV, V and V (lanes 14to 16) as well as the control strains (lanes 19 and 20). The rib geneprobe did hybridise with lower intensity to genomic DNA fragments fromstrains representing serotypes Ia, Ib, IV, VI, VII and serotype IIstrains 118/158 and 97/0057. This may indicate the presence of a gene inthese strains with a lower level of homology to rib. These hybridisingDNA fragments may contain a homologue of the GBS bca gene encoding theCa protein antigen which has been shown to be closely homologous to theRib protein (Wastfelt et al., J. Biol. Chem. 271:18892-18897 (1996)). Ifthis is the case, it would be in agreement with previous work whichshowed all strains of serotypes Ia, Ib, II and III to be positive forone the two proteins (Stalhammar-Carlemalm et al., 1993 [supra]).However, the apparent variable distribution of the rib gene amongstdifferent GBS serotypes, makes it a less than ideal candidate for use ina GBS vaccine that is cross-protective against all serotypes.

[0244] ID-65 (FIG. 13) Comment

[0245] The Southern blot analysis described in FIG. 13 indicates thatgene ID-65 is conserved across all GBS serotypes. The gene probehybridised specifically to a Hin DIII-digested genomic DNA fragment ofapproximately 3.0 kb in DNA digests from all GBS representatives, andwas absent from both the control strains (lanes 18 and 19). This wouldsuggest that the ID-65 gene is conserved across all GBS serotypes (andstrains) at both the gene and locus level. The ID-65 DNA probe alsohybridised weakly to the 1.636 bp molecular weight marker (the 1 kb DNAladder from NEB was used to estimate DNA fragment sizes in all Southernblot analyses).

[0246] ID-89 (FIG. 14) Comment

[0247] The Southern blot analysis described in FIG. 14 indicates thatgene ID-89 may not be conserved across all GBS serotypes. A 4.0 kbHinDIII-digested genomic DNA fragment from 12 out of 16 GBS strainshybridised specifically to the ID-89 gene probe. In addition, a 3.25 kbHinDIII-digested genomic DNA fragment from the GBS strain Ib (SB35)[lane 4) also hybridised specifically with the ID-89 gene probe.However, the ID-89 gene probe did not hybridise to digested genomic DNAfragments from strains Ia (515) [lane 2], IV (3139) [lane 13] and V(1169-NT) [lane 14], suggesting that these strains do not possess aID-89 gene homologue.

[0248] ID-93 (FIG. 15) Comment

[0249] The Southern blot analysis described in FIG. 15 indicates thatgene ID-93 is conserved across all GBS serotypes. The gene probehybridised specifically to a Hin DIII-digested genomic DNA fragment ofapproximately 3.25 kb in DNA digests from all GBS representatives, andwas absent from both the control strains (lanes 18 and 19). This wouldsuggest that the ID-93 gene is conserved across all GBS serotypes (andstrains) at both the gene and locus level.

[0250] ID-96 (FIG. 16) Comment

[0251] The Southern blot analysis described in FIG. 16 indicates thatgene ID-96 is conserved across all GBS serotypes. The gene probehybridised specifically to a Eco RI-digested genomic DNA fragment ofapproximately 12.0 kb in DNA digests from all GBS representatives, andwas absent from both the control strains (lanes 18 and 19). This wouldsuggest that the ID-96 gene is conserved across all GBS serotypes (andstrains) at both the gene and locus level.

1 276 1 1641 DNA Streptococcus agalactiae 1 gtgtttatga tgaaaaaaggacaagtaaat gatactaagc aatcttactc tctacgtaaa 60 tataaatttg gtttagcatcagtaatttta gggtcattca taatggtcac aagtcctgtt 120 tttgcggatc aaactacatcggttcaagtt aataatcaga caggcactag tgtggatgct 180 aataattctt ccaatgagacaagtgcgtca agtgtgatta cttccaataa tgatagtgtt 240 caagcgtctg ataaagttgtaaatagtcaa aatacggcaa caaaggacat tactactcct 300 ttagtagaga caaagccaatggtggaaaaa acattacctg aacaagggaa ttatgtttat 360 agcaaagaaa ccgaggtgaaaaatacacct tcaaaatcag ccccagtagc tttctatgca 420 aagaaaggtg ataaagttttctatgaccaa gtatttaata aagataatgt gaaatggatt 480 tcatataagt cttttggtggcgtacgtcga tacgcagcta ttgagtcact agatccatca 540 ggaggttcag agactaaagcacctactcct gtaacaaatt caggaagcaa taatcaagag 600 aaaatagcaa cgcaaggaaattatacattt tcacataaag tagaagtaaa aaatgaagct 660 aaggtagcga gtccaactcaatttacattg gacaaaggag acagaatttt ttacgaccaa 720 atactaacta ttgaaggaaatcagtggtta tcttataaat cattcaatgg tgttcgtcgt 780 tttgttttgc taggtaaagcatcttcagta gaaaaaactg aagataaaga aaaagtgtct 840 cctcaaccac aagcccgtattactaaaact ggtagactga ctatttctaa cgaaacaact 900 acaggttttg atattttaattacgaatatt aaagatgata acggtatcgc tgctgttaag 960 gtaccggttt ggactgaacaaggagggcaa gatgatatta aatggtatac agctgtaact 1020 actggggatg gcaactacaaagtagctgta tcatttgctg accataagaa tgagaagggt 1080 ctttataata ttcatttatactaccaagaa gctagtggga cacttgtagg tgtaacagga 1140 actaaagtga cagtagctggaactaattct tctcaagaac ctattgaaaa tggtttacca 1200 aagactggtg tttataatattatcggaagt actgaagtaa aaaatgaagc taaaatatca 1260 agtcagaccc aatttactttagaaaaaggt gacaaaataa attatgatca agtattgaca 1320 gcagatggtt accagtggatttcttacaaa tcttatagtg gtgttcgtcg ctatattcct 1380 gtgaaaaagc taactacaagtagtgaaaaa gcgaaagatg aggcgactaa accgactagt 1440 tatcccaact tacctaaaacaggtacctat acatttacta aaactgtaga tgtgaaaagt 1500 caacctaaag tatcaagtccagtggaattt aattttcaaa agggtgaaaa aatacattat 1560 gatcaagtgt tagtagtagatggtcatcag tggatttcat acaagagtta ttccggtatt 1620 cgtcgctata ttgaaattta a1641 2 546 PRT Streptococcus agalactiae 2 Met Phe Met Met Lys Lys GlyGln Val Asn Asp Thr Lys Gln Ser Tyr 1 5 10 15 Ser Leu Arg Lys Tyr LysPhe Gly Leu Ala Ser Val Ile Leu Gly Ser 20 25 30 Phe Ile Met Val Thr SerPro Val Phe Ala Asp Gln Thr Thr Ser Val 35 40 45 Gln Val Asn Asn Gln ThrGly Thr Ser Val Asp Ala Asn Asn Ser Ser 50 55 60 Asn Glu Thr Ser Ala SerSer Val Ile Thr Ser Asn Asn Asp Ser Val 65 70 75 80 Gln Ala Ser Asp LysVal Val Asn Ser Gln Asn Thr Ala Thr Lys Asp 85 90 95 Ile Thr Thr Pro LeuVal Glu Thr Lys Pro Met Val Glu Lys Thr Leu 100 105 110 Pro Glu Gln GlyAsn Tyr Val Tyr Ser Lys Glu Thr Glu Val Lys Asn 115 120 125 Thr Pro SerLys Ser Ala Pro Val Ala Phe Tyr Ala Lys Lys Gly Asp 130 135 140 Lys ValPhe Tyr Asp Gln Val Phe Asn Lys Asp Asn Val Lys Trp Ile 145 150 155 160Ser Tyr Lys Ser Phe Gly Gly Val Arg Arg Tyr Ala Ala Ile Glu Ser 165 170175 Leu Asp Pro Ser Gly Gly Ser Glu Thr Lys Ala Pro Thr Pro Val Thr 180185 190 Asn Ser Gly Ser Asn Asn Gln Glu Lys Ile Ala Thr Gln Gly Asn Tyr195 200 205 Thr Phe Ser His Lys Val Glu Val Lys Asn Glu Ala Lys Val AlaSer 210 215 220 Pro Thr Gln Phe Thr Leu Asp Lys Gly Asp Arg Ile Phe TyrAsp Gln 225 230 235 240 Ile Leu Thr Ile Glu Gly Asn Gln Trp Leu Ser TyrLys Ser Phe Asn 245 250 255 Gly Val Arg Arg Phe Val Leu Leu Gly Lys AlaSer Ser Val Glu Lys 260 265 270 Thr Glu Asp Lys Glu Lys Val Ser Pro GlnPro Gln Ala Arg Ile Thr 275 280 285 Lys Thr Gly Arg Leu Thr Ile Ser AsnGlu Thr Thr Thr Gly Phe Asp 290 295 300 Ile Leu Ile Thr Asn Ile Lys AspAsp Asn Gly Ile Ala Ala Val Lys 305 310 315 320 Val Pro Val Trp Thr GluGln Gly Gly Gln Asp Asp Ile Lys Trp Tyr 325 330 335 Thr Ala Val Thr ThrGly Asp Gly Asn Tyr Lys Val Ala Val Ser Phe 340 345 350 Ala Asp His LysAsn Glu Lys Gly Leu Tyr Asn Ile His Leu Tyr Tyr 355 360 365 Gln Glu AlaSer Gly Thr Leu Val Gly Val Thr Gly Thr Lys Val Thr 370 375 380 Val AlaGly Thr Asn Ser Ser Gln Glu Pro Ile Glu Asn Gly Leu Pro 385 390 395 400Lys Thr Gly Val Tyr Asn Ile Ile Gly Ser Thr Glu Val Lys Asn Glu 405 410415 Ala Lys Ile Ser Ser Gln Thr Gln Phe Thr Leu Glu Lys Gly Asp Lys 420425 430 Ile Asn Tyr Asp Gln Val Leu Thr Ala Asp Gly Tyr Gln Trp Ile Ser435 440 445 Tyr Lys Ser Tyr Ser Gly Val Arg Arg Tyr Ile Pro Val Lys LysLeu 450 455 460 Thr Thr Ser Ser Glu Lys Ala Lys Asp Glu Ala Thr Lys ProThr Ser 465 470 475 480 Tyr Pro Asn Leu Pro Lys Thr Gly Thr Tyr Thr PheThr Lys Thr Val 485 490 495 Asp Val Lys Ser Gln Pro Lys Val Ser Ser ProVal Glu Phe Asn Phe 500 505 510 Gln Lys Gly Glu Lys Ile His Tyr Asp GlnVal Leu Val Val Asp Gly 515 520 525 His Gln Trp Ile Ser Tyr Lys Ser TyrSer Gly Ile Arg Arg Tyr Ile 530 535 540 Glu Ile 545 3 822 DNAStreptococcus agalactiae 3 atgatattga gacgtcgaac tattgtttta tggcaactgggtatcgccat ttctctcatt 60 cttagtattc tagccttaaa tctttatttc catagtactcccttgcaaac caatgcagct 120 ttacggaacc ttgctccttc attaaaccat ctttttgggacagatggttt aggtagggat 180 atgtttgtca gaacgattaa aggactttat ttctctctacaagtcggctt attaggtgcc 240 cttatggggg tcattctggc gacagttttt ggagtgcttgcaggtttagg aaatagcatt 300 attgataaaa taatagcatg gttagttgat ttgtttattggtatgcctca tttgattttt 360 atgattctca tttcttttgt tgttgggaaa ggtgctcaaggggtcatcat tgcaacggct 420 gttacacatt ggccttcttt agcaaggctt atccgcaatgaagtctatca tctaaagaat 480 aaagaatttg tccaactttc taaaagtatg ggaaaaacgccttattatat tgtgaggcat 540 catatcctgc ctttgattgc ttctcaaatt ttcattggttttatcctctt atttccacat 600 gtcatcctac atgaagcatc aatgactttc ttaggatttgggctctctgc cgaacaacct 660 tcggttggta tcattctgtc agaggcagct aagcatatctctcttggaaa ttggtggttg 720 gttatctttc caggacttta tcttattttg gttgtcaatgcatttgatac tatcggagaa 780 tctttaaaga aactctttta ccctcaaact gatcattttt ag822 4 273 PRT Streptococcus agalactiae 4 Met Ile Leu Arg Arg Arg Thr IleVal Leu Trp Gln Leu Gly Ile Ala 1 5 10 15 Ile Ser Leu Ile Leu Ser IleLeu Ala Leu Asn Leu Tyr Phe His Ser 20 25 30 Thr Pro Leu Gln Thr Asn AlaAla Leu Arg Asn Leu Ala Pro Ser Leu 35 40 45 Asn His Leu Phe Gly Thr AspGly Leu Gly Arg Asp Met Phe Val Arg 50 55 60 Thr Ile Lys Gly Leu Tyr PheSer Leu Gln Val Gly Leu Leu Gly Ala 65 70 75 80 Leu Met Gly Val Ile LeuAla Thr Val Phe Gly Val Leu Ala Gly Leu 85 90 95 Gly Asn Ser Ile Ile AspLys Ile Ile Ala Trp Leu Val Asp Leu Phe 100 105 110 Ile Gly Met Pro HisLeu Ile Phe Met Ile Leu Ile Ser Phe Val Val 115 120 125 Gly Lys Gly AlaGln Gly Val Ile Ile Ala Thr Ala Val Thr His Trp 130 135 140 Pro Ser LeuAla Arg Leu Ile Arg Asn Glu Val Tyr His Leu Lys Asn 145 150 155 160 LysGlu Phe Val Gln Leu Ser Lys Ser Met Gly Lys Thr Pro Tyr Tyr 165 170 175Ile Val Arg His His Ile Leu Pro Leu Ile Ala Ser Gln Ile Phe Ile 180 185190 Gly Phe Ile Leu Leu Phe Pro His Val Ile Leu His Glu Ala Ser Met 195200 205 Thr Phe Leu Gly Phe Gly Leu Ser Ala Glu Gln Pro Ser Val Gly Ile210 215 220 Ile Leu Ser Glu Ala Ala Lys His Ile Ser Leu Gly Asn Trp TrpLeu 225 230 235 240 Val Ile Phe Pro Gly Leu Tyr Leu Ile Leu Val Val AsnAla Phe Asp 245 250 255 Thr Ile Gly Glu Ser Leu Lys Lys Leu Phe Tyr ProGln Thr Asp His 260 265 270 Phe 5 804 DNA Streptococcus agalactiae 5atgacagaaa cattattaag cattaaagac ctctccatca ccttcactca atacggaaga 60tttttaaaac catttcaatc aacaccgata caagcgctga atttagaaat taaaaaaggt 120gagttattag ctattatagg tgctagtggt tcggggaaga gtttattagc acatgctatt 180atggatattc ttcctaaaaa tgcatctgta acaggagata tgatttatcg tggtcaatca 240ctaaattcta aacgcattaa acagttgcga ggaaaagata ttacgttgat tccacaatca 300gttaattatt tagatccatc tatgaaagtc aaacatcagg tgcgcttagg tatctcagaa 360aattcaaagg ctactcaaga aggattgttt caacagtttg gtttaaaaga aagtgatggt 420gacttggatc ctttccaact ttctggcgga atgctccgac gtgttttgtt tacaacgtgt 480attagtgata aggtttcttt gattattgcg gatgagccca cccctggatt acatccagat 540gctctgcaaa tggttttaga ccaactacgc tcctttgcag ataaaggaat aagcgttata 600tttatcactc atgatattgt agcagctagt caaattgctg atcgtattac tatttttaaa 660gagggaaaag ctattgaaac agctccagct agtttcttta gcggaaatgg agagcagtta 720caaacagaat ttgctagaag tttatggcgc tctctcccac agcaagaatt tttgaaagga 780gttactcatg accttagagg ctaa 804 6 267 PRT Streptococcus agalactiae 6 MetThr Glu Thr Leu Leu Ser Ile Lys Asp Leu Ser Ile Thr Phe Thr 1 5 10 15Gln Tyr Gly Arg Phe Leu Lys Pro Phe Gln Ser Thr Pro Ile Gln Ala 20 25 30Leu Asn Leu Glu Ile Lys Lys Gly Glu Leu Leu Ala Ile Ile Gly Ala 35 40 45Ser Gly Ser Gly Lys Ser Leu Leu Ala His Ala Ile Met Asp Ile Leu 50 55 60Pro Lys Asn Ala Ser Val Thr Gly Asp Met Ile Tyr Arg Gly Gln Ser 65 70 7580 Leu Asn Ser Lys Arg Ile Lys Gln Leu Arg Gly Lys Asp Ile Thr Leu 85 9095 Ile Pro Gln Ser Val Asn Tyr Leu Asp Pro Ser Met Lys Val Lys His 100105 110 Gln Val Arg Leu Gly Ile Ser Glu Asn Ser Lys Ala Thr Gln Glu Gly115 120 125 Leu Phe Gln Gln Phe Gly Leu Lys Glu Ser Asp Gly Asp Leu AspPro 130 135 140 Phe Gln Leu Ser Gly Gly Met Leu Arg Arg Val Leu Phe ThrThr Cys 145 150 155 160 Ile Ser Asp Lys Val Ser Leu Ile Ile Ala Asp GluPro Thr Pro Gly 165 170 175 Leu His Pro Asp Ala Leu Gln Met Val Leu AspGln Leu Arg Ser Phe 180 185 190 Ala Asp Lys Gly Ile Ser Val Ile Phe IleThr His Asp Ile Val Ala 195 200 205 Ala Ser Gln Ile Ala Asp Arg Ile ThrIle Phe Lys Glu Gly Lys Ala 210 215 220 Ile Glu Thr Ala Pro Ala Ser PhePhe Ser Gly Asn Gly Glu Gln Leu 225 230 235 240 Gln Thr Glu Phe Ala ArgSer Leu Trp Arg Ser Leu Pro Gln Gln Glu 245 250 255 Phe Leu Lys Gly ValThr His Asp Leu Arg Gly 260 265 7 495 DNA Streptococcus agalactiae 7gtccatctgg ggtggttccc gattggtatt tcttctccga taggtacttt gagtcaagat 60attacgttag ctgatcgtat taagcacctt attttacctg ttttcacggt aagtattcta 120ggcattgcca atgtaactct tcatactaga actaaaatga tgtcggtact ttctagtgaa 180tatgtcttat ttgccagagc gcgtggggaa acggaatggc aaatttttaa aaatcattgt 240cttagaaatg ctatcgtacc agctattaca ctgcattttt cctattttgg agaattgttt 300ggaggatccg ttcttgctga gcaagttttc tcatatccag gactagggtc taccctaact 360gaagcaggac ttaaaagtga tacaccgcta cttctagcta ttgtgatgat agggacatta 420tttgtttttg cgggcaatct tattgcggat attttaaata gcataatcaa tccacagtta 480aggagaaaag tatga 495 8 164 PRT Streptococcus agalactiae 8 Val His LeuGly Trp Phe Pro Ile Gly Ile Ser Ser Pro Ile Gly Thr 1 5 10 15 Leu SerGln Asp Ile Thr Leu Ala Asp Arg Ile Lys His Leu Ile Leu 20 25 30 Pro ValPhe Thr Val Ser Ile Leu Gly Ile Ala Asn Val Thr Leu His 35 40 45 Thr ArgThr Lys Met Met Ser Val Leu Ser Ser Glu Tyr Val Leu Phe 50 55 60 Ala ArgAla Arg Gly Glu Thr Glu Trp Gln Ile Phe Lys Asn His Cys 65 70 75 80 LeuArg Asn Ala Ile Val Pro Ala Ile Thr Leu His Phe Ser Tyr Phe 85 90 95 GlyGlu Leu Phe Gly Gly Ser Val Leu Ala Glu Gln Val Phe Ser Tyr 100 105 110Pro Gly Leu Gly Ser Thr Leu Thr Glu Ala Gly Leu Lys Ser Asp Thr 115 120125 Pro Leu Leu Leu Ala Ile Val Met Ile Gly Thr Leu Phe Val Phe Ala 130135 140 Gly Asn Leu Ile Ala Asp Ile Leu Asn Ser Ile Ile Asn Pro Gln Leu145 150 155 160 Arg Arg Lys Val 9 579 DNA Streptococcus agalactiae 9ttgcggacaa ttacgttcaa acacaatgaa acgcgatcgt caaaaagcga aggtagggcg 60gtaatgctta aaagattatt tactgaagat ggggaattga caaagattag tcgtcgtttc 120gtttggatgt tagtggttat ctattgtctt attattgtca ggatgtgttt tgggcctcaa 180attatgattg agggggtatc aactccgaat gttcagcgct tcggaagaat tgtagctctt 240ttagtaccat ttaattcttt tcgtagttta gatcagctaa ctagctttaa agagattttt 300tgggttattg gtcaaaatgt agtgaatatt ttactgctgt ttcctctcat tatagggtta 360ctatccctaa agccaagttt acggaaatat aaaagcgtta tattacttgc tttcttgatg 420tctcttttca tagagtgtac tcaagttgtt ttagatattt taatagatgc taatcgggtt 480tttgaaatcg acgatctatg gacaaatacc ttaggcggtc ctttcgccct atggagttat 540cgaaacataa aaggttggct tctaactatt agaaaatga 579 10 192 PRT Streptococcusagalactiae 10 Met Arg Thr Ile Thr Phe Lys His Asn Glu Thr Arg Ser SerLys Ser 1 5 10 15 Glu Gly Arg Ala Val Met Leu Lys Arg Leu Phe Thr GluAsp Gly Glu 20 25 30 Leu Thr Lys Ile Ser Arg Arg Phe Val Trp Met Leu ValVal Ile Tyr 35 40 45 Cys Leu Ile Ile Val Arg Met Cys Phe Gly Pro Gln IleMet Ile Glu 50 55 60 Gly Val Ser Thr Pro Asn Val Gln Arg Phe Gly Arg IleVal Ala Leu 65 70 75 80 Leu Val Pro Phe Asn Ser Phe Arg Ser Leu Asp GlnLeu Thr Ser Phe 85 90 95 Lys Glu Ile Phe Trp Val Ile Gly Gln Asn Val ValAsn Ile Leu Leu 100 105 110 Leu Phe Pro Leu Ile Ile Gly Leu Leu Ser LeuLys Pro Ser Leu Arg 115 120 125 Lys Tyr Lys Ser Val Ile Leu Leu Ala PheLeu Met Ser Leu Phe Ile 130 135 140 Glu Cys Thr Gln Val Val Leu Asp IleLeu Ile Asp Ala Asn Arg Val 145 150 155 160 Phe Glu Ile Asp Asp Leu TrpThr Asn Thr Leu Gly Gly Pro Phe Ala 165 170 175 Leu Trp Ser Tyr Arg AsnIle Lys Gly Trp Leu Leu Thr Ile Arg Lys 180 185 190 11 1221 DNAStreptococcus agalactiae 11 ttgaaaaatt taaatcgtta tgtagttgcg gtttctggagtcgttttaca tttaatgcta 60 ggatcaactt atgcttggag tgtgtttcgt aacccaattatctcagagac tggttgggat 120 atttcatcag tttcattcgc ttttagtttg gctattttttgtctaggaat gtctgcagct 180 tttatgggac acttagtaga gcgttttggt cctaggataatgggaatgat ttctgctatt 240 ttatatggag cagggaatgt gttaacaggc ttagccattgaaactcagca gttatggtta 300 ctgtatgttg catacggtat tttaggagga atcggacttggttcaggtta tattactcca 360 gtatcgacta ttattaaatg gtttcctgat aggaggggactagcaacagg attcgctatt 420 atgggatttg gctttgcttc tttagtaaca agtccgcttgcacaatcctt actgattagg 480 attggtgtgg gtaaaacgtt ttatattttg ggattagtatatttttttgt catgatgatt 540 gcctcacaat ttattaaaca accacctcag gaaaaaataactattttgac tcacgatggt 600 aaaaagaatg ctatgaattc acaaattatc actggattaaaagcaaacgt cgctataaaa 660 tcaaaaacct tttacatcat ttggttgacc ttgtttattaatatttcgtg tggcttaggt 720 ttaatatcag cagcttcacc aatggcacaa gatttagcaggctattccgc agaatctgca 780 gccttattag taggggtact agggatattt aacggttttggacgtctgtt atgggcaagt 840 ctctctgact acattggacg cccgttgacc tttataatattatttattgt gaactttatt 900 atgacttcta gtttattttt gtcattcaat gctattgtatttgcaatagc gatgtctatt 960 ttaatgactt gttatggtgc aggtttttcc ttattacctgcttatctaag tgatattttt 1020 ggaacaaagg aattagctac tttacatggt tatagtttaacagcatgggc aatagcaggt 1080 ctgtttgggc ccctattgtt atcaaagaca tattcatggggaaattccta tcaattgaca 1140 ttaatggttt ttggtttttt attcttattc ggattattgttatctctata tttaagaaaa 1200 ttaacaacta aagttgtgta g 1221 12 406 PRTStreptococcus agalactiae 12 Leu Lys Asn Leu Asn Arg Tyr Val Val Ala ValSer Gly Val Val Leu 1 5 10 15 His Leu Met Leu Gly Ser Thr Tyr Ala TrpSer Val Phe Arg Asn Pro 20 25 30 Ile Ile Ser Glu Thr Gly Trp Asp Ile SerSer Val Ser Phe Ala Phe 35 40 45 Ser Leu Ala Ile Phe Cys Leu Gly Met SerAla Ala Phe Met Gly His 50 55 60 Leu Val Glu Arg Phe Gly Pro Arg Ile MetGly Met Ile Ser Ala Ile 65 70 75 80 Leu Tyr Gly Ala Gly Asn Val Leu ThrGly Leu Ala Ile Glu Thr Gln 85 90 95 Gln Leu Trp Leu Leu Tyr Val Ala TyrGly Ile Leu Gly Gly Ile Gly 100 105 110 Leu Gly Ser Gly Tyr Ile Thr ProVal Ser Thr Ile Ile Lys Trp Phe 115 120 125 Pro Asp Arg Arg Gly Leu AlaThr Gly Phe Ala Ile Met Gly Phe Gly 130 135 140 Phe Ala Ser Leu Val ThrSer Pro Leu Ala Gln Ser Leu Leu Ile Arg 145 150 155 160 Ile Gly Val GlyLys Thr Phe Tyr Ile Leu Gly Leu Val Tyr Phe Phe 165 170 175 Val Met MetIle Ala Ser Gln Phe Ile Lys Gln Pro Pro Gln Glu Lys 180 185 190 Ile ThrIle Leu Thr His Asp Gly Lys Lys Asn Ala Met Asn Ser Gln 195 200 205 IleIle Thr Gly Leu Lys Ala Asn Val Ala Ile Lys Ser Lys Thr Phe 210 215 220Tyr Ile Ile Trp Leu Thr Leu Phe Ile Asn Ile Ser Cys Gly Leu Gly 225 230235 240 Leu Ile Ser Ala Ala Ser Pro Met Ala Gln Asp Leu Ala Gly Tyr Ser245 250 255 Ala Glu Ser Ala Ala Leu Leu Val Gly Val Leu Gly Ile Phe AsnGly 260 265 270 Phe Gly Arg Leu Leu Trp Ala Ser Leu Ser Asp Tyr Ile GlyArg Pro 275 280 285 Leu Thr Phe Ile Ile Leu Phe Ile Val Asn Phe Ile MetThr Ser Ser 290 295 300 Leu Phe Leu Ser Phe Asn Ala Ile Val Phe Ala IleAla Met Ser Ile 305 310 315 320 Leu Met Thr Cys Tyr Gly Ala Gly Phe SerLeu Leu Pro Ala Tyr Leu 325 330 335 Ser Asp Ile Phe Gly Thr Lys Glu LeuAla Thr Leu His Gly Tyr Ser 340 345 350 Leu Thr Ala Trp Ala Ile Ala GlyLeu Phe Gly Pro Leu Leu Leu Ser 355 360 365 Lys Thr Tyr Ser Trp Gly AsnSer Tyr Gln Leu Thr Leu Met Val Phe 370 375 380 Gly Phe Leu Phe Leu PheGly Leu Leu Leu Ser Leu Tyr Leu Arg Lys 385 390 395 400 Leu Thr Thr LysVal Val 405 13 303 DNA Streptococcus agalactiae 13 atggcagata aaaacagaacatttaaactt gtaggtgcag gatcttctag cacacaagaa 60 aaaattgaaa agcctgctctttcgtttatg caagatgcgt ggcgtcgctt gaaaaaaaac 120 aaattagcag tagtttcactctatttatta gctcttttac ttactttttc gttagcctca 180 aatttatttg taactcagaaggatgctaat gggtttgatt cgaaaaaagt aacgacatat 240 cgcaacttac cacctaaattgagttcaaac cttccttttt ggaatggtag cattaatcca 300 tca 303 14 101 PRTStreptococcus agalactiae 14 Met Ala Asp Lys Asn Arg Thr Phe Lys Leu ValGly Ala Gly Ser Ser 1 5 10 15 Ser Thr Gln Glu Lys Ile Glu Lys Pro AlaLeu Ser Phe Met Gln Asp 20 25 30 Ala Trp Arg Arg Leu Lys Lys Asn Lys LeuAla Val Val Ser Leu Tyr 35 40 45 Leu Leu Ala Leu Leu Leu Thr Phe Ser LeuAla Ser Asn Leu Phe Val 50 55 60 Thr Gln Lys Asp Ala Asn Gly Phe Asp SerLys Lys Val Thr Thr Tyr 65 70 75 80 Arg Asn Leu Pro Pro Lys Leu Ser SerAsn Leu Pro Phe Trp Asn Gly 85 90 95 Ser Ile Asn Pro Ser 100 15 678 DNAStreptococcus agalactiae 15 atgaaaatag tagtaccagt aatgcctcgc agtcttgaagaggctcaaga aatagattta 60 tcaaaatttg atagtgttga tattattgaa tggcgagctgatgccttacc aaaggatgac 120 attattaatg tagctccagc tatttttgag aaattcgcaggtcatgaaat tatttttact 180 tttcgtacaa cgcgtgaagg tggtaatatt gtcttatctgatgctgagta tgttgagtta 240 atccagaaaa ttaattctat ctacaatcca gattatattgattttgagta tttttcacat 300 aaagaagttt ttcaagaaat gctagaattt ccaaatttagtcctgtctta tcacaatttt 360 caagagacac cggagaatat tatggagata ttttcagaattaacagccct agcaccacga 420 gttgtgaaaa tcgcagtaat gccaaagaat gaacaagatgtcttagacgt tatgaattac 480 actcgcggtt tcaagactat taatcctgat caagtttatgcgacggtatc tatgagtaaa 540 attggacgta tttctcgttt tgctggtgat gtaactggatctagttggac atttgcatat 600 ttagattcat ctatcgcacc cggacaaatt actatttcagagatgaagcg tgtcaaagca 660 ttgcttgacg ctgactga 678 16 225 PRTStreptococcus agalactiae 16 Met Lys Ile Val Val Pro Val Met Pro Arg SerLeu Glu Glu Ala Gln 1 5 10 15 Glu Ile Asp Leu Ser Lys Phe Asp Ser ValAsp Ile Ile Glu Trp Arg 20 25 30 Ala Asp Ala Leu Pro Lys Asp Asp Ile IleAsn Val Ala Pro Ala Ile 35 40 45 Phe Glu Lys Phe Ala Gly His Glu Ile IlePhe Thr Phe Arg Thr Thr 50 55 60 Arg Glu Gly Gly Asn Ile Val Leu Ser AspAla Glu Tyr Val Glu Leu 65 70 75 80 Ile Gln Lys Ile Asn Ser Ile Tyr AsnPro Asp Tyr Ile Asp Phe Glu 85 90 95 Tyr Phe Ser His Lys Glu Val Phe GlnGlu Met Leu Glu Phe Pro Asn 100 105 110 Leu Val Leu Ser Tyr His Asn PheGln Glu Thr Pro Glu Asn Ile Met 115 120 125 Glu Ile Phe Ser Glu Leu ThrAla Leu Ala Pro Arg Val Val Lys Ile 130 135 140 Ala Val Met Pro Lys AsnGlu Gln Asp Val Leu Asp Val Met Asn Tyr 145 150 155 160 Thr Arg Gly PheLys Thr Ile Asn Pro Asp Gln Val Tyr Ala Thr Val 165 170 175 Ser Met SerLys Ile Gly Arg Ile Ser Arg Phe Ala Gly Asp Val Thr 180 185 190 Gly SerSer Trp Thr Phe Ala Tyr Leu Asp Ser Ser Ile Ala Pro Gly 195 200 205 GlnIle Thr Ile Ser Glu Met Lys Arg Val Lys Ala Leu Leu Asp Ala 210 215 220Asp 225 17 333 DNA Streptococcus agalactiae 17 atgaaagact tatttgcaacaacagaagca tcatcaagga aacaggaaca agatagaatt 60 gtcaattaca taaaacaacatgttgagtta acaaatggta atcaaataaa aaaaattgag 120 tttatcgact ttcaaaaaaatgagatgaca ggtacatggg gaatttctac taaaattaat 180 gaacaatttt cgattagtttttctgaagat agaattggtg gtaaacttag agcattagga 240 tatcaaccga atgaaataggtttttcaaag gacatcaata gtaataatca aaatgttaat 300 gatattgaag tgatttatatgaagaaagaa tag 333 18 110 PRT Streptococcus agalactiae 18 Met Lys AspLeu Phe Ala Thr Thr Glu Ala Ser Ser Arg Lys Gln Glu 1 5 10 15 Gln AspArg Ile Val Asn Tyr Ile Lys Gln His Val Glu Leu Thr Asn 20 25 30 Gly AsnGln Ile Lys Lys Ile Glu Phe Ile Asp Phe Gln Lys Asn Glu 35 40 45 Met ThrGly Thr Trp Gly Ile Ser Thr Lys Ile Asn Glu Gln Phe Ser 50 55 60 Ile SerPhe Ser Glu Asp Arg Ile Gly Gly Lys Leu Arg Ala Leu Gly 65 70 75 80 TyrGln Pro Asn Glu Ile Gly Phe Ser Lys Asp Ile Asn Ser Asn Asn 85 90 95 GlnAsn Val Asn Asp Ile Glu Val Ile Tyr Met Lys Lys Glu 100 105 110 19 350DNA Streptococcus agalactiae 19 atgaaaaaac gtatatggta tttgataataataatcacag taattttagg aggactagcc 60 atgaaaaact tatttgcaac aacagaagcatcatcaagga aacaggaaca agatagaatt 120 gtcaattaca taaaacaaca tgttgagttaacaaatggta atcaaataaa aaaaattgag 180 tttatcgact ttcaaaaaaa tgagatgacaggtacatggg gaatttctac taaaattaat 240 gaacaatttt cgattagttt ttctgaagatagaattggtg gtaaacttag agcattagga 300 tatcaaccga atgaaatagg tttttcaaaggacatcaata gtaataatca 350 20 117 PRT Streptococcus agalactiae 20 Met LysLys Arg Ile Trp Tyr Leu Ile Ile Ile Ile Thr Val Ile Leu 1 5 10 15 GlyGly Leu Ala Met Lys Asn Leu Phe Ala Thr Thr Glu Ala Ser Ser 20 25 30 ArgLys Gln Glu Gln Asp Arg Ile Val Asn Tyr Ile Lys Gln His Val 35 40 45 GluLeu Thr Asn Gly Asn Gln Ile Lys Lys Ile Glu Phe Ile Asp Phe 50 55 60 GlnLys Asn Glu Met Thr Gly Thr Trp Gly Ile Ser Thr Lys Ile Asn 65 70 75 80Glu Gln Phe Ser Ile Ser Phe Ser Glu Asp Arg Ile Gly Gly Lys Leu 85 90 95Arg Ala Leu Gly Tyr Gln Pro Asn Glu Ile Gly Phe Ser Lys Asp Ile 100 105110 Asn Ser Asn Asn Gln 115 21 1350 DNA Streptococcus agalactiae 21atgtcaaatc aatatgatta tatcgttatt ggtggaggta gtgcaggcag tggtaccgct 60aatagggcag ccatgtatgg agcaaaagtc ctgttaattg aaggtggaca agtaggtgga 120acttgtgtta acttaggttg tgtacctaag aaaatcatgt ggtatggtgc acaagtttct 180gagacactcc ataagtatag ttcaggttat ggttttgaag ccaataatct tagttttgat 240tttactactc taaaagctaa tcgcgatgct tacgtgcagc ggtctagaca gtcgtatgcc 300gctaattttg agcgtaatgg ggtcgaaaag attgatggat ttgctcgttt tattgataac 360catactattg aagtgaatgg tcagcaatat aaagctcctc acattactat tgcaacaggt 420ggacaccctc tttaccctga tattattgga agtgaacttg gtgagacttc tgatgatttt 480tttggatggg agaccttacc aaattctata ttgattgttg gggcgggcta tatcgcggca 540gaacttgctg gagtggttaa tgaattaggc gttgaaaccc atcttgcatt tagaaaagac 600catattctac gcggatttga tgacatggta acaagtgagg ttatggctga aatggagaaa 660tcaggtatct ctttacatgc taaccatgta cctaaatctc ttaaacgcga tgaaggtggc 720aagttgattt ttgaagctga aaatgggaaa acgcttgtcg ttgatcgtgt aatatgggct 780atcggccgtg gaccaaatgt agacatggga cttgaaaata ccgatattgt tttaaatgat 840aaagattata tcaaaacaga tgaatttgag aatacttctg tagatggcgt gtatgctatt 900ggagatgtta atgggaaaat tgccttgaca ccggtagcaa ttgcagcagg tcgtcgctta 960tcagaaagac tttttaatca taaagataac gaaaaattag attaccataa tgtaccttca 1020gttattttta ctcaccctgt aattgggacg gtaggacttt cagaagcagc agctatcgag 1080caatttggaa aagataatat caaagtctat acatcaactt ttacctctat gtatacggct 1140gttaccagta atcgccaagc agttaagatg aagctcataa ccctaggaaa agaggaaaaa 1200gttattgggc ttcatggtgt tggttatggt attgatgaaa tgattcaagg tttttcagtt 1260gctatcaaaa tgggggctac taaagcagac tttgatgata ctgttgctat tcacccaact 1320ggatctgagg aatttgttac aatgcgctaa 1350 22 449 PRT Streptococcusagalactiae 22 Met Ser Asn Gln Tyr Asp Tyr Ile Val Ile Gly Gly Gly SerAla Gly 1 5 10 15 Ser Gly Thr Ala Asn Arg Ala Ala Met Tyr Gly Ala LysVal Leu Leu 20 25 30 Ile Glu Gly Gly Gln Val Gly Gly Thr Cys Val Asn LeuGly Cys Val 35 40 45 Pro Lys Lys Ile Met Trp Tyr Gly Ala Gln Val Ser GluThr Leu His 50 55 60 Lys Tyr Ser Ser Gly Tyr Gly Phe Glu Ala Asn Asn LeuSer Phe Asp 65 70 75 80 Phe Thr Thr Leu Lys Ala Asn Arg Asp Ala Tyr ValGln Arg Ser Arg 85 90 95 Gln Ser Tyr Ala Ala Asn Phe Glu Arg Asn Gly ValGlu Lys Ile Asp 100 105 110 Gly Phe Ala Arg Phe Ile Asp Asn His Thr IleGlu Val Asn Gly Gln 115 120 125 Gln Tyr Lys Ala Pro His Ile Thr Ile AlaThr Gly Gly His Pro Leu 130 135 140 Tyr Pro Asp Ile Ile Gly Ser Glu LeuGly Glu Thr Ser Asp Asp Phe 145 150 155 160 Phe Gly Trp Glu Thr Leu ProAsn Ser Ile Leu Ile Val Gly Ala Gly 165 170 175 Tyr Ile Ala Ala Glu LeuAla Gly Val Val Asn Glu Leu Gly Val Glu 180 185 190 Thr His Leu Ala PheArg Lys Asp His Ile Leu Arg Gly Phe Asp Asp 195 200 205 Met Val Thr SerGlu Val Met Ala Glu Met Glu Lys Ser Gly Ile Ser 210 215 220 Leu His AlaAsn His Val Pro Lys Ser Leu Lys Arg Asp Glu Gly Gly 225 230 235 240 LysLeu Ile Phe Glu Ala Glu Asn Gly Lys Thr Leu Val Val Asp Arg 245 250 255Val Ile Trp Ala Ile Gly Arg Gly Pro Asn Val Asp Met Gly Leu Glu 260 265270 Asn Thr Asp Ile Val Leu Asn Asp Lys Asp Tyr Ile Lys Thr Asp Glu 275280 285 Phe Glu Asn Thr Ser Val Asp Gly Val Tyr Ala Ile Gly Asp Val Asn290 295 300 Gly Lys Ile Ala Leu Thr Pro Val Ala Ile Ala Ala Gly Arg ArgLeu 305 310 315 320 Ser Glu Arg Leu Phe Asn His Lys Asp Asn Glu Lys LeuAsp Tyr His 325 330 335 Asn Val Pro Ser Val Ile Phe Thr His Pro Val IleGly Thr Val Gly 340 345 350 Leu Ser Glu Ala Ala Ala Ile Glu Gln Phe GlyLys Asp Asn Ile Lys 355 360 365 Val Tyr Thr Ser Thr Phe Thr Ser Met TyrThr Ala Val Thr Ser Asn 370 375 380 Arg Gln Ala Val Lys Met Lys Leu IleThr Leu Gly Lys Glu Glu Lys 385 390 395 400 Val Ile Gly Leu His Gly ValGly Tyr Gly Ile Asp Glu Met Ile Gln 405 410 415 Gly Phe Ser Val Ala IleLys Met Gly Ala Thr Lys Ala Asp Phe Asp 420 425 430 Asp Thr Val Ala IleHis Pro Thr Gly Ser Glu Glu Phe Val Thr Met 435 440 445 Arg 23 3168 DNAStreptococcus agalactiae 23 atgacaaaaa aacatcttaa aacgcttgcc ttggcacttactacagtatc agtagtgaca 60 tacagccagg aggtatatgg attagaaaga gaggaatcggtcaaacaaga acaaacccag 120 tcagcttcag aagatgattg gttcgaagaa gataatgagaggaaaacaaa tgtttctaaa 180 gagaattcta ctgttgatga aacagttagt gatttattttctgatggaaa tagtaataac 240 tctagttcta aaaccgagtc agtggtaagt gaccctaaacaagtccccaa agcaaaacca 300 gaggttacac aagaagcaag caattctagt aatgatgctagcaaagtaga agtaccaaaa 360 caggatacag cttcaaaaaa ggaaactcta gaaacatcaacttgggaggc aaaagatttc 420 gtaactagag gggatacttt agtaggtttt tcaaaatctggaattaataa gttatctcaa 480 acatcacact tggttttacc aagtcatgca gcagatggaactcaattgac acaagtagct 540 agctttgctt ttactccaga taaaaagacg gccattgcagaatatacaag taggctagga 600 gaaaatggga aaccgagtcg tttagatatt gatcagaaggaaattattga tgagggagaa 660 atatttaatg cttaccagtt gactaagctt actattccaaatggttataa gtctattggt 720 caagatgctt ttgtggacaa taagaatatt gctgaggttaaccttcctga gagtctcgag 780 actatttcag actatgcttt tgctcacatg tctttaaaacaagtaaagtt accagataac 840 ctaaaggtca ttggagaatt agcttttttt gataatcagattggtggtaa gctttacttg 900 ccacgtcact tgataaaatt agcagaacgc gctttcaaatctaatcgtat tcaaacagtt 960 gaatttttgg gaagtaagct taaggttata ggagaagcaagttttcaaga taataatctg 1020 aggaatgtta tgcttccgga tggacttgaa aaaatagaatcagaagcttt tacaggaaat 1080 ccaggagatg aacattacaa caatcaggtt gtattgcgcacaaggacagg ccaaaatcca 1140 catcaacttg cgactgagaa tacttacgtc aatccggacaaatcattgtg gcgtgcaaca 1200 cctgatatgg attataccaa atggttagag gaagattttacctatcaaaa aaatagtgtt 1260 acaggttttt caaataaagg cttacaaaag gtaagacgtaataaaaactt agaaattcca 1320 aaacaacaca atggtattac tattactgaa attggtgataacgcttttcg caatgttgat 1380 tttcaaagta aaactttacg taaatatgat ttggaagaaataaagctccc ctcaactatt 1440 cggaaaatag gtgcttttgc ttttcaatct aataacttgaaatcctttga agcaagtgaa 1500 gatttagaag agattaaaga gggagccttt atgaataatcgtattggaac tctagacttg 1560 aaagacaaac ttatcaaaat aggtgatgct gctttccatattaatcatat ttatgccatt 1620 gttcttccag aatctgtaca agaaatagga cgttcagcttttcgacaaaa tggtgcgctt 1680 caccttatgt ttatcggaaa taaggttaaa acaattggtgaaatggcttt tttatccaat 1740 aaactggaaa gtgtaaatct ctctgagcaa aaacaattaaagacaattga ggtccaagct 1800 ttttcggata atgcccttag tgaagtagtc ttaccgccaaatttacagac tattcgtgaa 1860 gaggctttca aaaggaatca tttgaaagaa gtgaagggttcatctacatt atctcagatt 1920 acttttaatg cttttgatca aaatgatggg gacaaacgctttggtaagaa agtggttgtt 1980 aggacacata ataattctca tatgttagca gatggtgagcgttttatcat tgatccagat 2040 aagctatctt ctacaatggt agaccttgaa aaggttttaaaaataatcga aggtttagat 2100 tactctacat tacgtcagac tactcaaact cagtttagagaaatgactac tgcaggtaaa 2160 gcgttgttat caaaatctaa cctccgacaa ggagaaaaacaaaaattcct tcaagaagca 2220 caatttttcc ttggtcgcgt tgatttggat aaagccatagctaaagctga gaaggcttta 2280 gtgaccaaga aggcaacaaa gaatggtcat ttgcttgagaggagtattaa caaagcggta 2340 ttagcttata ataatagtgc tattaaaaaa gctaatgttaagcgcttgga aaaagagtta 2400 gacttgctga cagatttagt cgagggaaaa ggaccattagcgcaagctac aatggtacaa 2460 ggagtttatt tattaaagac gcctttacca ttgccagaatattatatcgg attgaacgtt 2520 tattttgaca agtctggaaa attgatttat gcacttgatatgagtgatac tattggcgag 2580 ggacaaaaag atgcatatgg taatcctata ttaaatgttgacgaggataa tgaaggttat 2640 cataccttgg cagttgccac tttagctgat tatgaaggtctttatattaa agatatttta 2700 aatagttccc ttgataagat taaagcaata cgccagattcctttggcaaa atatcataga 2760 ttaggaattt tccaagctat ccgaaatgca gcggcagaagcagaccgatt gcttcctaag 2820 acacctaagg ggtacctaaa tgaagtccca aattatcgtaaaaaacaaat ggagaaaaat 2880 ttaaaaccag ttgattataa aacgccgatt tttaataaggctttacctaa tgaaaaggta 2940 gacggtgata gagcggctaa aggtcataat ataaatgcggagactaataa ttctgtagct 3000 gtaacaccaa taaggtccga gcagcaatta cataagtcacagtctgatgt aaatttacct 3060 caaacaagtt ctaaaaataa ttttatatac gagattctaggatacgttag tttatgtttg 3120 cttttcctag taactgctgg gaaaaaagga aaacgagcaagaaaataa 3168 24 1055 PRT Streptococcus agalactiae 24 Met Thr Lys LysHis Leu Lys Thr Leu Ala Leu Ala Leu Thr Thr Val 1 5 10 15 Ser Val ValThr Tyr Ser Gln Glu Val Tyr Gly Leu Glu Arg Glu Glu 20 25 30 Ser Val LysGln Glu Gln Thr Gln Ser Ala Ser Glu Asp Asp Trp Phe 35 40 45 Glu Glu AspAsn Glu Arg Lys Thr Asn Val Ser Lys Glu Asn Ser Thr 50 55 60 Val Asp GluThr Val Ser Asp Leu Phe Ser Asp Gly Asn Ser Asn Asn 65 70 75 80 Ser SerSer Lys Thr Glu Ser Val Val Ser Asp Pro Lys Gln Val Pro 85 90 95 Lys AlaLys Pro Glu Val Thr Gln Glu Ala Ser Asn Ser Ser Asn Asp 100 105 110 AlaSer Lys Val Glu Val Pro Lys Gln Asp Thr Ala Ser Lys Lys Glu 115 120 125Thr Leu Glu Thr Ser Thr Trp Glu Ala Lys Asp Phe Val Thr Arg Gly 130 135140 Asp Thr Leu Val Gly Phe Ser Lys Ser Gly Ile Asn Lys Leu Ser Gln 145150 155 160 Thr Ser His Leu Val Leu Pro Ser His Ala Ala Asp Gly Thr GlnLeu 165 170 175 Thr Gln Val Ala Ser Phe Ala Phe Thr Pro Asp Lys Lys ThrAla Ile 180 185 190 Ala Glu Tyr Thr Ser Arg Leu Gly Glu Asn Gly Lys ProSer Arg Leu 195 200 205 Asp Ile Asp Gln Lys Glu Ile Ile Asp Glu Gly GluIle Phe Asn Ala 210 215 220 Tyr Gln Leu Thr Lys Leu Thr Ile Pro Asn GlyTyr Lys Ser Ile Gly 225 230 235 240 Gln Asp Ala Phe Val Asp Asn Lys AsnIle Ala Glu Val Asn Leu Pro 245 250 255 Glu Ser Leu Glu Thr Ile Ser AspTyr Ala Phe Ala His Met Ser Leu 260 265 270 Lys Gln Val Lys Leu Pro AspAsn Leu Lys Val Ile Gly Glu Leu Ala 275 280 285 Phe Phe Asp Asn Gln IleGly Gly Lys Leu Tyr Leu Pro Arg His Leu 290 295 300 Ile Lys Leu Ala GluArg Ala Phe Lys Ser Asn Arg Ile Gln Thr Val 305 310 315 320 Glu Phe LeuGly Ser Lys Leu Lys Val Ile Gly Glu Ala Ser Phe Gln 325 330 335 Asp AsnAsn Leu Arg Asn Val Met Leu Pro Asp Gly Leu Glu Lys Ile 340 345 350 GluSer Glu Ala Phe Thr Gly Asn Pro Gly Asp Glu His Tyr Asn Asn 355 360 365Gln Val Val Leu Arg Thr Arg Thr Gly Gln Asn Pro His Gln Leu Ala 370 375380 Thr Glu Asn Thr Tyr Val Asn Pro Asp Lys Ser Leu Trp Arg Ala Thr 385390 395 400 Pro Asp Met Asp Tyr Thr Lys Trp Leu Glu Glu Asp Phe Thr TyrGln 405 410 415 Lys Asn Ser Val Thr Gly Phe Ser Asn Lys Gly Leu Gln LysVal Arg 420 425 430 Arg Asn Lys Asn Leu Glu Ile Pro Lys Gln His Asn GlyIle Thr Ile 435 440 445 Thr Glu Ile Gly Asp Asn Ala Phe Arg Asn Val AspPhe Gln Ser Lys 450 455 460 Thr Leu Arg Lys Tyr Asp Leu Glu Glu Ile LysLeu Pro Ser Thr Ile 465 470 475 480 Arg Lys Ile Gly Ala Phe Ala Phe GlnSer Asn Asn Leu Lys Ser Phe 485 490 495 Glu Ala Ser Glu Asp Leu Glu GluIle Lys Glu Gly Ala Phe Met Asn 500 505 510 Asn Arg Ile Gly Thr Leu AspLeu Lys Asp Lys Leu Ile Lys Ile Gly 515 520 525 Asp Ala Ala Phe His IleAsn His Ile Tyr Ala Ile Val Leu Pro Glu 530 535 540 Ser Val Gln Glu IleGly Arg Ser Ala Phe Arg Gln Asn Gly Ala Leu 545 550 555 560 His Leu MetPhe Ile Gly Asn Lys Val Lys Thr Ile Gly Glu Met Ala 565 570 575 Phe LeuSer Asn Lys Leu Glu Ser Val Asn Leu Ser Glu Gln Lys Gln 580 585 590 LeuLys Thr Ile Glu Val Gln Ala Phe Ser Asp Asn Ala Leu Ser Glu 595 600 605Val Val Leu Pro Pro Asn Leu Gln Thr Ile Arg Glu Glu Ala Phe Lys 610 615620 Arg Asn His Leu Lys Glu Val Lys Gly Ser Ser Thr Leu Ser Gln Ile 625630 635 640 Thr Phe Asn Ala Phe Asp Gln Asn Asp Gly Asp Lys Arg Phe GlyLys 645 650 655 Lys Val Val Val Arg Thr His Asn Asn Ser His Met Leu AlaAsp Gly 660 665 670 Glu Arg Phe Ile Ile Asp Pro Asp Lys Leu Ser Ser ThrMet Val Asp 675 680 685 Leu Glu Lys Val Leu Lys Ile Ile Glu Gly Leu AspTyr Ser Thr Leu 690 695 700 Arg Gln Thr Thr Gln Thr Gln Phe Arg Glu MetThr Thr Ala Gly Lys 705 710 715 720 Ala Leu Leu Ser Lys Ser Asn Leu ArgGln Gly Glu Lys Gln Lys Phe 725 730 735 Leu Gln Glu Ala Gln Phe Phe LeuGly Arg Val Asp Leu Asp Lys Ala 740 745 750 Ile Ala Lys Ala Glu Lys AlaLeu Val Thr Lys Lys Ala Thr Lys Asn 755 760 765 Gly His Leu Leu Glu ArgSer Ile Asn Lys Ala Val Leu Ala Tyr Asn 770 775 780 Asn Ser Ala Ile LysLys Ala Asn Val Lys Arg Leu Glu Lys Glu Leu 785 790 795 800 Asp Leu LeuThr Asp Leu Val Glu Gly Lys Gly Pro Leu Ala Gln Ala 805 810 815 Thr MetVal Gln Gly Val Tyr Leu Leu Lys Thr Pro Leu Pro Leu Pro 820 825 830 GluTyr Tyr Ile Gly Leu Asn Val Tyr Phe Asp Lys Ser Gly Lys Leu 835 840 845Ile Tyr Ala Leu Asp Met Ser Asp Thr Ile Gly Glu Gly Gln Lys Asp 850 855860 Ala Tyr Gly Asn Pro Ile Leu Asn Val Asp Glu Asp Asn Glu Gly Tyr 865870 875 880 His Thr Leu Ala Val Ala Thr Leu Ala Asp Tyr Glu Gly Leu TyrIle 885 890 895 Lys Asp Ile Leu Asn Ser Ser Leu Asp Lys Ile Lys Ala IleArg Gln 900 905 910 Ile Pro Leu Ala Lys Tyr His Arg Leu Gly Ile Phe GlnAla Ile Arg 915 920 925 Asn Ala Ala Ala Glu Ala Asp Arg Leu Leu Pro LysThr Pro Lys Gly 930 935 940 Tyr Leu Asn Glu Val Pro Asn Tyr Arg Lys LysGln Met Glu Lys Asn 945 950 955 960 Leu Lys Pro Val Asp Tyr Lys Thr ProIle Phe Asn Lys Ala Leu Pro 965 970 975 Asn Glu Lys Val Asp Gly Asp ArgAla Ala Lys Gly His Asn Ile Asn 980 985 990 Ala Glu Thr Asn Asn Ser ValAla Val Thr Pro Ile Arg Ser Glu Gln 995 1000 1005 Gln Leu His Lys SerGln Ser Asp Val Asn Leu Pro Gln Thr Ser 1010 1015 1020 Ser Lys Asn AsnPhe Ile Tyr Glu Ile Leu Gly Tyr Val Ser Leu 1025 1030 1035 Cys Leu LeuPhe Leu Val Thr Ala Gly Lys Lys Gly Lys Arg Ala 1040 1045 1050 Arg Lys1055 25 153 DNA Streptococcus agalactiae 25 gcaggataca tcatgcacaagcacgaggct atcgtgtcat gctggggtca acccaggaag 60 acatgtcggc acaagctgaagatttcttta cagtctgtac acaataaaga gacgggtaag 120 agcgctttta atgacaaagaacgactagca att 153 26 51 PRT Streptococcus agalactiae 26 Ala Gly Tyr IleMet His Lys His Glu Ala Ile Val Ser Cys Trp Gly 1 5 10 15 Gln Pro ArgLys Thr Cys Arg His Lys Leu Lys Ile Ser Leu Gln Ser 20 25 30 Val His AsnLys Glu Thr Gly Lys Ser Ala Phe Asn Asp Lys Glu Arg 35 40 45 Leu Ala Ile50 27 1095 DNA Streptococcus agalactiae 27 gtgtcattta tgcaaagaaaatcctattta aaatccatga gtgttcttac tttaacagct 60 tgtcttatat caggatatgtggttaaagat attgctatgt tacatgcagt atctgccagt 120 gagaagaaag caaataatgtcagtccgaga gaaaatctct acagggctgt caatgataat 180 tggctagcca atacaaaactcaaacaaggg cagactagtg ttaatagttt ttcagaaatt 240 gaggataaat taaagcaactgttagtgtct gatatggcta aaatggcctc aggaaagatt 300 gaaacaacca atgatgaacagaaaaaaatg gttgcatact ataaacaagg tatggacttt 360 aaaacaagag ataaaaatggtctcaaacct ctaaaaccag ttttacaaaa acttgaagca 420 gtctcttcaa tgaaagactttcaaagtttg gcccatgatt ttgtgatgag tggttttgtt 480 ttaccatttg gtttgactgtggaaaccaat gctcgagata atagccaaaa gcaattggtg 540 cttcgtcaag cacccgcattacttgaatca cctgaccaat ataagaaggg caataaagaa 600 ggtgaggcta aattatcagcttaccgtact tcagcaatgg ctttgcttaa acaagctgga 660 aaaagtaaca ttgaagatagaaaactagtt aaacaagcta tagcatttga tagactctta 720 tcagaaaaaa cgcaagttgatcaaagtaaa atcacagctg aaagtgagac agctgcgggg 780 cgatataacc ctgaaagtatggaaacggtt cacaattacg ccaaggaatt tgactttaaa 840 gaattgattg aaaaactagttgggccaacg aataaggcag tcaatgtaga agataaaact 900 tattttaaac aggttaatgatgttataaat agtaaacaat tagccaatat gaaagcatgg 960 atgatgattt ctatgctagttgatcaatca gattttctag gagaacaaaa tcgtcaagca 1020 gcgagtgctt ttaagaatgttgcgtctggt ttgactcaga ttgaatcgaa agaaaaaatg 1080 cttacaccca attag 109528 364 PRT Streptococcus agalactiae 28 Met Ser Phe Met Gln Arg Lys SerTyr Leu Lys Ser Met Ser Val Leu 1 5 10 15 Thr Leu Thr Ala Cys Leu IleSer Gly Tyr Val Val Lys Asp Ile Ala 20 25 30 Met Leu His Ala Val Ser AlaSer Glu Lys Lys Ala Asn Asn Val Ser 35 40 45 Pro Arg Glu Asn Leu Tyr ArgAla Val Asn Asp Asn Trp Leu Ala Asn 50 55 60 Thr Lys Leu Lys Gln Gly GlnThr Ser Val Asn Ser Phe Ser Glu Ile 65 70 75 80 Glu Asp Lys Leu Lys GlnLeu Leu Val Ser Asp Met Ala Lys Met Ala 85 90 95 Ser Gly Lys Ile Glu ThrThr Asn Asp Glu Gln Lys Lys Met Val Ala 100 105 110 Tyr Tyr Lys Gln GlyMet Asp Phe Lys Thr Arg Asp Lys Asn Gly Leu 115 120 125 Lys Pro Leu LysPro Val Leu Gln Lys Leu Glu Ala Val Ser Ser Met 130 135 140 Lys Asp PheGln Ser Leu Ala His Asp Phe Val Met Ser Gly Phe Val 145 150 155 160 LeuPro Phe Gly Leu Thr Val Glu Thr Asn Ala Arg Asp Asn Ser Gln 165 170 175Lys Gln Leu Val Leu Arg Gln Ala Pro Ala Leu Leu Glu Ser Pro Asp 180 185190 Gln Tyr Lys Lys Gly Asn Lys Glu Gly Glu Ala Lys Leu Ser Ala Tyr 195200 205 Arg Thr Ser Ala Met Ala Leu Leu Lys Gln Ala Gly Lys Ser Asn Ile210 215 220 Glu Asp Arg Lys Leu Val Lys Gln Ala Ile Ala Phe Asp Arg LeuLeu 225 230 235 240 Ser Glu Lys Thr Gln Val Asp Gln Ser Lys Ile Thr AlaGlu Ser Glu 245 250 255 Thr Ala Ala Gly Arg Tyr Asn Pro Glu Ser Met GluThr Val His Asn 260 265 270 Tyr Ala Lys Glu Phe Asp Phe Lys Glu Leu IleGlu Lys Leu Val Gly 275 280 285 Pro Thr Asn Lys Ala Val Asn Val Glu AspLys Thr Tyr Phe Lys Gln 290 295 300 Val Asn Asp Val Ile Asn Ser Lys GlnLeu Ala Asn Met Lys Ala Trp 305 310 315 320 Met Met Ile Ser Met Leu ValAsp Gln Ser Asp Phe Leu Gly Glu Gln 325 330 335 Asn Arg Gln Ala Ala SerAla Phe Lys Asn Val Ala Ser Gly Leu Thr 340 345 350 Gln Ile Glu Ser LysGlu Lys Met Leu Thr Pro Asn 355 360 29 174 DNA Streptococcus agalactiae29 atggaaatgc ctaaaagaaa tgaattactc aataaagaaa ttaaaatgag tattgataaa 60cttagatata aagaaccaga gagtgaacat gacaagcgac ctacttttta tttggtagta 120cttatacttg ttactgtagc agttatattg tcgttattta aatatttttt atag 174 30 57PRT Streptococcus agalactiae 30 Met Glu Met Pro Lys Arg Asn Glu Leu LeuAsn Lys Glu Ile Lys Met 1 5 10 15 Ser Ile Asp Lys Leu Arg Tyr Lys GluPro Glu Ser Glu His Asp Lys 20 25 30 Arg Pro Thr Phe Tyr Leu Val Val LeuIle Leu Val Thr Val Ala Val 35 40 45 Ile Leu Ser Leu Phe Lys Tyr Phe Leu50 55 31 140 DNA Streptococcus agalactiae 31 atgcaggtat ttttaaatattgtcaataaa ttctttgatc cagttattca tatgggttcg 60 ggagttgtga tgctaattgtcatgacaggt ttagccatga tatttggagt gaagttttct 120 aaagcacttg aaggtggtat140 32 46 PRT Streptococcus agalactiae 32 Met Gln Val Phe Leu Asn IleVal Asn Lys Phe Phe Asp Pro Val Ile 1 5 10 15 His Met Gly Ser Gly ValVal Met Leu Ile Val Met Thr Gly Leu Ala 20 25 30 Met Ile Phe Gly Val LysPhe Ser Lys Ala Leu Glu Gly Gly 35 40 45 33 110 DNA Streptococcusagalactiae 33 atgaaaaaga aaacattcag tgcttataac tttttaacgg ctcttatcctttgtcttttg 60 acagtgcttt ttatctttcc attttattgg attatgacag gagcttttaa 11034 36 PRT Streptococcus agalactiae 34 Met Lys Lys Lys Thr Phe Ser AlaTyr Asn Phe Leu Thr Ala Leu Ile 1 5 10 15 Leu Cys Leu Leu Thr Val LeuPhe Ile Phe Pro Phe Tyr Trp Ile Met 20 25 30 Thr Gly Ala Phe 35 35 744DNA Streptococcus agalactiae 35 atgactgaga actggttaca tactaaagatggttcagata tttattatcg tgtcgttggt 60 caaggtcaac cgattgtttt tttacatggcaatagcttaa gtagtcgcta ttttgataag 120 caaatagcat atttttctaa gtattaccaagttattgtta tggatagtag agggcatggc 180 aaaagtcatg caaagctaaa taccattagtttcaggcaaa tagcagttga cttaaaggat 240 atcttagttc atttagagat tgataaagttatattggtag gccatagcga tggtgctaat 300 ttagctttag tttttcaaac gatgtttccagatatggtta gagggctttt gcttaattca 360 gggaacctga ctattcatgg tcagcgatggtgggatattc ttttagtaag gattgcctat 420 aaattccttc actatttagg gaaactctttccgtatatga ggcaaaaagc tcaagttatt 480 tcgcttatgt tggaggattt gaagattagtccagctgatt tacagcatgt gtcaactcct 540 gtaatggttt tggttggaaa taaggacataattaagttaa atcattctaa gaaacttgct 600 tcttattttc caagggggga gttttattctttagttggct ttgggcatca cattattaag 660 caagattccc atgtttttaa tattattgcaaaaaagttta tcaacgatac gttgaaagga 720 gaaattgttg aaaaagctaa ttga 744 36247 PRT Streptococcus agalactiae 36 Met Thr Glu Asn Trp Leu His Thr LysAsp Gly Ser Asp Ile Tyr Tyr 1 5 10 15 Arg Val Val Gly Gln Gly Gln ProIle Val Phe Leu His Gly Asn Ser 20 25 30 Leu Ser Ser Arg Tyr Phe Asp LysGln Ile Ala Tyr Phe Ser Lys Tyr 35 40 45 Tyr Gln Val Ile Val Met Asp SerArg Gly His Gly Lys Ser His Ala 50 55 60 Lys Leu Asn Thr Ile Ser Phe ArgGln Ile Ala Val Asp Leu Lys Asp 65 70 75 80 Ile Leu Val His Leu Glu IleAsp Lys Val Ile Leu Val Gly His Ser 85 90 95 Asp Gly Ala Asn Leu Ala LeuVal Phe Gln Thr Met Phe Pro Asp Met 100 105 110 Val Arg Gly Leu Leu LeuAsn Ser Gly Asn Leu Thr Ile His Gly Gln 115 120 125 Arg Trp Trp Asp IleLeu Leu Val Arg Ile Ala Tyr Lys Phe Leu His 130 135 140 Tyr Leu Gly LysLeu Phe Pro Tyr Met Arg Gln Lys Ala Gln Val Ile 145 150 155 160 Ser LeuMet Leu Glu Asp Leu Lys Ile Ser Pro Ala Asp Leu Gln His 165 170 175 ValSer Thr Pro Val Met Val Leu Val Gly Asn Lys Asp Ile Ile Lys 180 185 190Leu Asn His Ser Lys Lys Leu Ala Ser Tyr Phe Pro Arg Gly Glu Phe 195 200205 Tyr Ser Leu Val Gly Phe Gly His His Ile Ile Lys Gln Asp Ser His 210215 220 Val Phe Asn Ile Ile Ala Lys Lys Phe Ile Asn Asp Thr Leu Lys Gly225 230 235 240 Glu Ile Val Glu Lys Ala Asn 245 37 405 DNA Streptococcusagalactiae 37 atggtagcaa aagagttagg taaaaatagc tttactatcc caactatttgttctaattgc 60 tccgcaggta ctgccattgc agttgtatat aatgatgacc attctttcttaagatacggc 120 tatcccgagt ctccacttca tatttttatc aatacacgga tcattgcacaggcaccaagc 180 aaatattttt gggctggtat tggggacggt atttcaaaag cccctgaagtagaacgtgct 240 accttagagg ctaagaccaa taaactacca catactgcag tgttaggacaagcagtcgct 300 ctgtcttcaa aggaagcttt ttatcaattt ggtgaacaag gtctaaaagacgttgaagct 360 aatttagctt cgcgtgcagt tgaagaaatt gcgcttgata tctta 405 38135 PRT Streptococcus agalactiae 38 Met Val Ala Lys Glu Leu Gly Lys AsnSer Phe Thr Ile Pro Thr Ile 1 5 10 15 Cys Ser Asn Cys Ser Ala Gly ThrAla Ile Ala Val Val Tyr Asn Asp 20 25 30 Asp His Ser Phe Leu Arg Tyr GlyTyr Pro Glu Ser Pro Leu His Ile 35 40 45 Phe Ile Asn Thr Arg Ile Ile AlaGln Ala Pro Ser Lys Tyr Phe Trp 50 55 60 Ala Gly Ile Gly Asp Gly Ile SerLys Ala Pro Glu Val Glu Arg Ala 65 70 75 80 Thr Leu Glu Ala Lys Thr AsnLys Leu Pro His Thr Ala Val Leu Gly 85 90 95 Gln Ala Val Ala Leu Ser SerLys Glu Ala Phe Tyr Gln Phe Gly Glu 100 105 110 Gln Gly Leu Lys Asp ValGlu Ala Asn Leu Ala Ser Arg Ala Val Glu 115 120 125 Glu Ile Ala Leu AspIle Leu 130 135 39 921 DNA Streptococcus agalactiae 39 ttgagggaaacttactggaa aatttcaagc gattgcgata aaataaatct tgcagagttt 60 tctagagaaaggaggtcaga tttattggag tggcaagatc tagcgcagtt acctgtatct 120 atttttaaagactatgttac agatgctcaa gacgcggaaa aaccttttat atggacagaa 180 gtatttttaagggagattaa tcgctcaaat caagaaatta ttttgcatat ttggccgatg 240 actaagacagtcattctggg gatgttagat cgagaattac cacatttaga attagctaaa 300 aaagaaatcatcagtcgtgg ttatgaacca gttgttcgga attttggagg tctcgcagtt 360 gtagctgatgaaggaatttt aaatttttca ttggttattc cagatgtttt tgagagaaaa 420 ttgtctatctcagatgggta tcttataatg gtcgatttta ttagaagtat attttcggat 480 ttttatcaacctattgagca ctttgaagta gagacctcct attgtcctgg taagtttgat 540 cttagtataaatggcaaaaa atttgctggc ttggctcagc gccgtataaa gaatggtatt 600 gcggtatcaatttaccttag cgtttgtggc gatcaaaaag ggcggagtca aatgatttca 660 gatttttataagattggtct aggtgatacg ggtagtccaa ttgcttatcc aaatgtagat 720 cctgaaattatggctaatct atctgatcta ttagattgtc ctatgacagt agaagatgtt 780 attgatcgtatgttgattag ccttaaacaa gtaggtttta atgatcgttt actgatgatt 840 agacccgatttagttgcaga gtttgataga tttcaggcta agtctatggc taataagggg 900 atggtgagcagagatgaata a 921 40 306 PRT Streptococcus agalactiae 40 Met Arg Glu ThrTyr Trp Lys Ile Ser Ser Asp Cys Asp Lys Ile Asn 1 5 10 15 Leu Ala GluPhe Ser Arg Glu Arg Arg Ser Asp Leu Leu Glu Trp Gln 20 25 30 Asp Leu AlaGln Leu Pro Val Ser Ile Phe Lys Asp Tyr Val Thr Asp 35 40 45 Ala Gln AspAla Glu Lys Pro Phe Ile Trp Thr Glu Val Phe Leu Arg 50 55 60 Glu Ile AsnArg Ser Asn Gln Glu Ile Ile Leu His Ile Trp Pro Met 65 70 75 80 Thr LysThr Val Ile Leu Gly Met Leu Asp Arg Glu Leu Pro His Leu 85 90 95 Glu LeuAla Lys Lys Glu Ile Ile Ser Arg Gly Tyr Glu Pro Val Val 100 105 110 ArgAsn Phe Gly Gly Leu Ala Val Val Ala Asp Glu Gly Ile Leu Asn 115 120 125Phe Ser Leu Val Ile Pro Asp Val Phe Glu Arg Lys Leu Ser Ile Ser 130 135140 Asp Gly Tyr Leu Ile Met Val Asp Phe Ile Arg Ser Ile Phe Ser Asp 145150 155 160 Phe Tyr Gln Pro Ile Glu His Phe Glu Val Glu Thr Ser Tyr CysPro 165 170 175 Gly Lys Phe Asp Leu Ser Ile Asn Gly Lys Lys Phe Ala GlyLeu Ala 180 185 190 Gln Arg Arg Ile Lys Asn Gly Ile Ala Val Ser Ile TyrLeu Ser Val 195 200 205 Cys Gly Asp Gln Lys Gly Arg Ser Gln Met Ile SerAsp Phe Tyr Lys 210 215 220 Ile Gly Leu Gly Asp Thr Gly Ser Pro Ile AlaTyr Pro Asn Val Asp 225 230 235 240 Pro Glu Ile Met Ala Asn Leu Ser AspLeu Leu Asp Cys Pro Met Thr 245 250 255 Val Glu Asp Val Ile Asp Arg MetLeu Ile Ser Leu Lys Gln Val Gly 260 265 270 Phe Asn Asp Arg Leu Leu MetIle Arg Pro Asp Leu Val Ala Glu Phe 275 280 285 Asp Arg Phe Gln Ala LysSer Met Ala Asn Lys Gly Met Val Ser Arg 290 295 300 Asp Glu 305 41 867DNA Streptococcus agalactiae 41 ttggaaggtt tacttattgc attgattcccatgtttgcgt gggaaagtat tggatttgtt 60 agtaataaaa ttggagggcg tccaaatcaacaaacatttg gaatgacttt aggagcattg 120 ctatttgcga ttatcgtatg gttatttaaacagccagaga tgactgcctc attgtggatt 180 tttggtatct taggtggtat cctatggtcagtcggccaaa atggtcaatt tcaagcaatg 240 aaatatatgg gagtctctgt tgctaatccactgtcaagtg gtgcacaatt agtaggtgga 300 agcctagttg gtgctttagt ctttcatgaatggactaagc caatccaatt tattttagga 360 ttgacagcgt tgacattatt agttatcggcttctatttct caagtaaacg tgatgtttca 420 gaacaagctt tggcaacaca tcaagagttttcaaaaggat ttgctacaat tgcttattca 480 actgtaggtt acatctcgta cgcagttttatttaacaaca ttatgaagtt cgacgctatg 540 gccgtcattt tacccatggc tgttggaatgtgtctaggtg caatttgttt catgaagttt 600 cgtgttaact ttgaggctgt tgttgttaaaaatatgatta caggtctcat gtggggcgtt 660 ggtaatgtct tcatgttatt ggcagcagctaaagcagggc tagcaattgc ttttagtttt 720 tctcaacttg gagtaattat ctctattattggtggtattt tatttttagg tgagacaaaa 780 acgaagaaag agcagaaatg ggttgtcatgggtatccttt gttttgttat gggtgctata 840 ttacttggta ttgttaaatc ttattaa 86742 288 PRT Streptococcus agalactiae 42 Met Glu Gly Leu Leu Ile Ala LeuIle Pro Met Phe Ala Trp Glu Ser 1 5 10 15 Ile Gly Phe Val Ser Asn LysIle Gly Gly Arg Pro Asn Gln Gln Thr 20 25 30 Phe Gly Met Thr Leu Gly AlaLeu Leu Phe Ala Ile Ile Val Trp Leu 35 40 45 Phe Lys Gln Pro Glu Met ThrAla Ser Leu Trp Ile Phe Gly Ile Leu 50 55 60 Gly Gly Ile Leu Trp Ser ValGly Gln Asn Gly Gln Phe Gln Ala Met 65 70 75 80 Lys Tyr Met Gly Val SerVal Ala Asn Pro Leu Ser Ser Gly Ala Gln 85 90 95 Leu Val Gly Gly Ser LeuVal Gly Ala Leu Val Phe His Glu Trp Thr 100 105 110 Lys Pro Ile Gln PheIle Leu Gly Leu Thr Ala Leu Thr Leu Leu Val 115 120 125 Ile Gly Phe TyrPhe Ser Ser Lys Arg Asp Val Ser Glu Gln Ala Leu 130 135 140 Ala Thr HisGln Glu Phe Ser Lys Gly Phe Ala Thr Ile Ala Tyr Ser 145 150 155 160 ThrVal Gly Tyr Ile Ser Tyr Ala Val Leu Phe Asn Asn Ile Met Lys 165 170 175Phe Asp Ala Met Ala Val Ile Leu Pro Met Ala Val Gly Met Cys Leu 180 185190 Gly Ala Ile Cys Phe Met Lys Phe Arg Val Asn Phe Glu Ala Val Val 195200 205 Val Lys Asn Met Ile Thr Gly Leu Met Trp Gly Val Gly Asn Val Phe210 215 220 Met Leu Leu Ala Ala Ala Lys Ala Gly Leu Ala Ile Ala Phe SerPhe 225 230 235 240 Ser Gln Leu Gly Val Ile Ile Ser Ile Ile Gly Gly IleLeu Phe Leu 245 250 255 Gly Glu Thr Lys Thr Lys Lys Glu Gln Lys Trp ValVal Met Gly Ile 260 265 270 Leu Cys Phe Val Met Gly Ala Ile Leu Leu GlyIle Val Lys Ser Tyr 275 280 285 43 960 DNA Streptococcus agalactiae 43atgacaactt actacgaagc tataaactgg aacgaaattg aagatgttat tgataaatca 60acttgggaaa aactaaccga acaattttgg ctcgatacac gtatcccttt atcaaatgac 120ttagacgatt ggcgcaaact ttccgctcaa gaaaaagatc ttgttggcaa ggtttttgga 180ggcttaaccc tacttgatac catgcaatca gaaactggtg ttgaagctat tcgtgccgat 240gttcgcacgc ctcacgaaga agctgtctta aacaatattc aattcatgga atctgttcac 300gctaaatctt attcttcaat tttctcaact ttaaatacta aatcagaaat tgaagaaatt 360ttcgagtgga ctaataataa tgagttcctt caagaaaaag cacgtattat caatgacatt 420tatgctaatg gaaatgccct tcaaaaaaag gtggcttcca cctacctcga aactttcctt 480ttttattctg gctttttcac acctctttac tatttgggaa ataataagtt agcaaatgtt 540gctgaaatca ttaaattaat tattcgtgat gaatctgtac atggtactta tatcggttac 600aaattccagc ttggttttaa cgaattacca gaagatgagc aagagaattt tcgtgattgg 660atgtatgacc tcctttatca gctgtatgaa aacgaagaaa aatacaccaa gacactttat 720gatggcgtag gatggactga agaagttatg acctttttac gctacaatgc taataaagct 780cttatgaatt taggacaaga tcctttattc ccagatacag caaatgatgt caacccaatt 840gttatgaatg gtatttcaac aggaacatca aaccatgact tcttctctca agtaggtaat 900ggttacctac ttggtagcgt tgaagctatg catgatgatg actataacta tggattataa 960 44319 PRT Streptococcus agalactiae 44 Met Thr Thr Tyr Tyr Glu Ala Ile AsnTrp Asn Glu Ile Glu Asp Val 1 5 10 15 Ile Asp Lys Ser Thr Trp Glu LysLeu Thr Glu Gln Phe Trp Leu Asp 20 25 30 Thr Arg Ile Pro Leu Ser Asn AspLeu Asp Asp Trp Arg Lys Leu Ser 35 40 45 Ala Gln Glu Lys Asp Leu Val GlyLys Val Phe Gly Gly Leu Thr Leu 50 55 60 Leu Asp Thr Met Gln Ser Glu ThrGly Val Glu Ala Ile Arg Ala Asp 65 70 75 80 Val Arg Thr Pro His Glu GluAla Val Leu Asn Asn Ile Gln Phe Met 85 90 95 Glu Ser Val His Ala Lys SerTyr Ser Ser Ile Phe Ser Thr Leu Asn 100 105 110 Thr Lys Ser Glu Ile GluGlu Ile Phe Glu Trp Thr Asn Asn Asn Glu 115 120 125 Phe Leu Gln Glu LysAla Arg Ile Ile Asn Asp Ile Tyr Ala Asn Gly 130 135 140 Asn Ala Leu GlnLys Lys Val Ala Ser Thr Tyr Leu Glu Thr Phe Leu 145 150 155 160 Phe TyrSer Gly Phe Phe Thr Pro Leu Tyr Tyr Leu Gly Asn Asn Lys 165 170 175 LeuAla Asn Val Ala Glu Ile Ile Lys Leu Ile Ile Arg Asp Glu Ser 180 185 190Val His Gly Thr Tyr Ile Gly Tyr Lys Phe Gln Leu Gly Phe Asn Glu 195 200205 Leu Pro Glu Asp Glu Gln Glu Asn Phe Arg Asp Trp Met Tyr Asp Leu 210215 220 Leu Tyr Gln Leu Tyr Glu Asn Glu Glu Lys Tyr Thr Lys Thr Leu Tyr225 230 235 240 Asp Gly Val Gly Trp Thr Glu Glu Val Met Thr Phe Leu ArgTyr Asn 245 250 255 Ala Asn Lys Ala Leu Met Asn Leu Gly Gln Asp Pro LeuPhe Pro Asp 260 265 270 Thr Ala Asn Asp Val Asn Pro Ile Val Met Asn GlyIle Ser Thr Gly 275 280 285 Thr Ser Asn His Asp Phe Phe Ser Gln Val GlyAsn Gly Tyr Leu Leu 290 295 300 Gly Ser Val Glu Ala Met His Asp Asp AspTyr Asn Tyr Gly Leu 305 310 315 45 311 DNA Streptococcus agalactiae 45atgaattggt cacgtatctg ggaactcgta aaaattaata tcctttattc aaaccctcag 60actctatcgg cactaagaaa aaagcaagaa aagcatccta aaaaagaatt ttcagcttat 120aaatccatgt ttagaaatca gttatttcag attttgctct tttcaataat ttatgtattt 180ctctttgtat cacttgattt taaagaatat ccgggctatt tcacgttcta cattggtatc 240tttacactag tatccattat ctactctttt attgcgatgt acagtgtttt ctatgagagt 300gacgatgtta a 311 46 103 PRT Streptococcus agalactiae 46 Met Asn Trp SerArg Ile Trp Glu Leu Val Lys Ile Asn Ile Leu Tyr 1 5 10 15 Ser Asn ProGln Thr Leu Ser Ala Leu Arg Lys Lys Gln Glu Lys His 20 25 30 Pro Lys LysGlu Phe Ser Ala Tyr Lys Ser Met Phe Arg Asn Gln Leu 35 40 45 Phe Gln IleLeu Leu Phe Ser Ile Ile Tyr Val Phe Leu Phe Val Ser 50 55 60 Leu Asp PheLys Glu Tyr Pro Gly Tyr Phe Thr Phe Tyr Ile Gly Ile 65 70 75 80 Phe ThrLeu Val Ser Ile Ile Tyr Ser Phe Ile Ala Met Tyr Ser Val 85 90 95 Phe TyrGlu Ser Asp Asp Val 100 47 312 DNA Streptococcus agalactiae 47taatctttta gtcaacggag caacaggaaa attgcaggct atgcgacaga tattccacca 60cataatttag cagaagtcat tgatgctgtc gtgtacatga ttgatcaccc taaagctaaa 120ttagataaat taatggaatt tctacctggt ccagattttc caactggcgc tatcattcaa 180ggaaaagatg aaattcgtaa ggcatatgag actggtaagg ggagagtagc ggttcgctcg 240cgaactgcta ttgaaacctt aaaaggtggt aagaaacaaa ttattgttac tgaaattcct 300tatgaagtta at 312 48 103 PRT Streptococcus agalactiae 48 Ser Phe Ser GlnArg Ser Asn Arg Lys Ile Ala Gly Tyr Ala Thr Asp 1 5 10 15 Ile Pro ProHis Asn Leu Ala Glu Val Ile Asp Ala Val Val Tyr Met 20 25 30 Ile Asp HisPro Lys Ala Lys Leu Asp Lys Leu Met Glu Phe Leu Pro 35 40 45 Gly Pro AspPhe Pro Thr Gly Ala Ile Ile Gln Gly Lys Asp Glu Ile 50 55 60 Arg Lys AlaTyr Glu Thr Gly Lys Gly Arg Val Ala Val Arg Ser Arg 65 70 75 80 Thr AlaIle Glu Thr Leu Lys Gly Gly Lys Lys Gln Ile Ile Val Thr 85 90 95 Glu IlePro Tyr Glu Val Asn 100 49 654 DNA Streptococcus agalactiae 49atgggacgta agtgggccaa tattgttgcc aaaaagactg ctaaagatgg tgctaactca 60aaagtatacg ctaaattcgg tgttgaaata tatgttgctg caaagcaagg tgaaccagac 120cccgagtcaa actcagctct aaaattcgtt ttggaccgtg ctaagcaagc acaagttcca 180aagcatgtta ttgataaagc gattgataaa gccaaaggaa acacagatga aactttcgta 240gagggacgct atgaaggttt tggtccaaat ggttcaatga ttattgtgga tactttgaca 300tcaaatgtta accgtacggc agcaaatgta cgtactgctt acggtaagaa cggtggcaat 360atgggagctt caggatcggt atcctactta tttgataaaa aaggtgtcat cgtttttgct 420ggtgatgatg ctgacactgt cttcgaacaa ttacttgaag cggatgtaga cgtagatgat 480gttgaagcag aagagggaac aataacagtt tataccgccc caacagatct tcataaaggt 540atccaagcac ttcgcgataa tggtgtagaa gaattccaag ttactgaact tgaaatgatt 600cctcaatcag aagtagtatt ggaaggtgat gaccttgaaa cttttgaaaa gctt 654 50 218PRT Streptococcus agalactiae 50 Met Gly Arg Lys Trp Ala Asn Ile Val AlaLys Lys Thr Ala Lys Asp 1 5 10 15 Gly Ala Asn Ser Lys Val Tyr Ala LysPhe Gly Val Glu Ile Tyr Val 20 25 30 Ala Ala Lys Gln Gly Glu Pro Asp ProGlu Ser Asn Ser Ala Leu Lys 35 40 45 Phe Val Leu Asp Arg Ala Lys Gln AlaGln Val Pro Lys His Val Ile 50 55 60 Asp Lys Ala Ile Asp Lys Ala Lys GlyAsn Thr Asp Glu Thr Phe Val 65 70 75 80 Glu Gly Arg Tyr Glu Gly Phe GlyPro Asn Gly Ser Met Ile Ile Val 85 90 95 Asp Thr Leu Thr Ser Asn Val AsnArg Thr Ala Ala Asn Val Arg Thr 100 105 110 Ala Tyr Gly Lys Asn Gly GlyAsn Met Gly Ala Ser Gly Ser Val Ser 115 120 125 Tyr Leu Phe Asp Lys LysGly Val Ile Val Phe Ala Gly Asp Asp Ala 130 135 140 Asp Thr Val Phe GluGln Leu Leu Glu Ala Asp Val Asp Val Asp Asp 145 150 155 160 Val Glu AlaGlu Glu Gly Thr Ile Thr Val Tyr Thr Ala Pro Thr Asp 165 170 175 Leu HisLys Gly Ile Gln Ala Leu Arg Asp Asn Gly Val Glu Glu Phe 180 185 190 GlnVal Thr Glu Leu Glu Met Ile Pro Gln Ser Glu Val Val Leu Glu 195 200 205Gly Asp Asp Leu Glu Thr Phe Glu Lys Leu 210 215 51 135 DNA Streptococcusagalactiae 51 ttggagaaat atttgaagaa cccgattaca tggattggat tagttcttgtggttacgtgg 60 tttttaacta aaagtagtga atttttgatt tttggtgtgt gtgtcttgttgttagtattt 120 gctagtcaaa gtgat 135 52 45 PRT Streptococcus agalactiae52 Met Glu Lys Tyr Leu Lys Asn Pro Ile Thr Trp Ile Gly Leu Val Leu 1 510 15 Val Val Thr Trp Phe Leu Thr Lys Ser Ser Glu Phe Leu Ile Phe Gly 2025 30 Val Cys Val Leu Leu Leu Val Phe Ala Ser Gln Ser Asp 35 40 45 53318 DNA Streptococcus agalactiae 53 atgacacaat cagatgcata tctctcgttgaacgcgaaga cacgctttag agatcgcaca 60 ggtaattatc attttacttc ggataaagaggctgttgaac aatatatgat agaacatgtt 120 gaacctaata cgatggtgtt cacatcactaattgaaaagc tagattattt ggtttctaat 180 aactactatg aatcggacct tctaaaacaatataaccttg agtttatttg ccaaattttt 240 gagcatgcat acgctaagaa atttgcttttctaaatttta tgggggcttt aaaattttat 300 aatgcttatg ctcttaat 318 54 106 PRTStreptococcus agalactiae 54 Met Thr Gln Ser Asp Ala Tyr Leu Ser Leu AsnAla Lys Thr Arg Phe 1 5 10 15 Arg Asp Arg Thr Gly Asn Tyr His Phe ThrSer Asp Lys Glu Ala Val 20 25 30 Glu Gln Tyr Met Ile Glu His Val Glu ProAsn Thr Met Val Phe Thr 35 40 45 Ser Leu Ile Glu Lys Leu Asp Tyr Leu ValSer Asn Asn Tyr Tyr Glu 50 55 60 Ser Asp Leu Leu Lys Gln Tyr Asn Leu GluPhe Ile Cys Gln Ile Phe 65 70 75 80 Glu His Ala Tyr Ala Lys Lys Phe AlaPhe Leu Asn Phe Met Gly Ala 85 90 95 Leu Lys Phe Tyr Asn Ala Tyr Ala LeuAsn 100 105 55 2451 DNA Streptococcus agalactiae 55 atggtatttatggcaaataa gaaaaaaaca aaaggaaaga aaaccagaag acctactaag 60 gcagaaatagagcgtcaaag agctattcaa aggatgatta ctgctcttgt tttaacaatt 120 attctcttctttggtattat cagattaggt atttttggta ttacagtcta taacgtcatc 180 cgttttatggtaggtagctt ggcttactta tttattgcgg caactttaat ctacctttat 240 ttctttaaatggttgcgaaa gaaagatagc ttagtagcag gttttttgat agcttcttta 300 ggattattgattgagtggca tgcttacctt ttctcaatgc ctattttgaa agataaagaa 360 attttgcgttcaactgctcg attaattgtg tctgatttaa tgcaatttaa aatcactgtt 420 tttgccggtggaggtatgtt gggtgctttg atttacaagc caattgcttt tctcttttct 480 aatattggtgcctatatgat tggtgttctc ttcatcattt tgggtctctt tttaatgagt 540 tctctggaagtttatgacat cgtcgaattt attagagctt ttaaaaataa agtggcagag 600 aagcacgagcaaaataaaaa ggagcgtttt gctaagcgag agatgaaaaa agcaatcgct 660 gaacaagagcgcatagagcg tcaaaaagct gaagaagaag cttatttagc ttcggttaat 720 gtagaccctgaaacgggtga gattctagag gatcaagctg aggacaattt ggatgatgcg 780 ctaccacctgaggtaagtga aacatcaact ccggtatttg agccagagat ccttgcttat 840 gagacatcgcctcaaaatga tcctttacca gtagagccga caatttattt agaagactat 900 gattcgccgattcctaatat gagagaaaat gatgaggaaa tggtttatga tttagatgat 960 gatgtagatgatagtgatat agaaaatgtc gactttacac ctaaaacgac actggtttat 1020 aaattaccaacgatagattt atttgcacca gataagccta aaaatcaatc caaagaaaag 1080 gatttagtccgaaagaatat cagagtttta gaagaaacat ttagaagttt tggtatcgat 1140 gtaaaagtagaacgtgctga aattggacca tcagttacta aatatgaaat taaaccagca 1200 gttggagttcgtgtgaatcg tatttcaaat ctatctgacg acctagctct tgctcttgca 1260 gcaaaagatgtgcgtataga agcaccaatt cctggaaaat cattaatagg tattgaagtt 1320 cctaactcagaaattgcaac ggtttctttc cgcgaacttt gggaacaatc tgatgccaat 1380 cctgaaaaccttttagaagt accactagga aaagctgtta acggcaatgc tcgcagtttt 1440 aacttagctagaatgccgca tcttttggta gctggttcaa ctggttcagg taaatctgtg 1500 gcagttaatggaattatttc aagtattttg atgaaggcac gtccagatca agttaagttt 1560 atgatgattgatcccaaaat ggttgaatta tctgtttata atgatattcc acatttatta 1620 atccctgttgtaaccaatcc gcgtaaagca agtaaggcac tccaaaaagt tgttgatgaa 1680 atggaaaatcgatacgagtt atttagcaaa attggtgtgc gtaatatagc aggttataat 1740 acaaaggttgaagagtttaa tgcttcctct gagcaaaaac aaatgccttt gcctttaatc 1800 gttgtcattgtagatgaatt ggctgacttg atgatggttg ctagtaaaga agttgaagat 1860 gctattattcgtttggggca aaaagcacgt gctgcaggta tccatatgat tcttgcaact 1920 caacgtccatccgtagatgt tatttctggt ttgattaaag caaatgttcc gtcgcgtatt 1980 gcatttgctgtttcaagtgg tactgatagc cgtacgatcc ttgatgaaaa tggtgctgaa 2040 aagctcttgggacggggtga catgctcttt aagcctattg atgagaatca tccagtacga 2100 ctacaaggttcctttatttc agatgatgat gttgaaagga tcgttggttt tatcaaagac 2160 caagccgaggctgactatga tgatgccttt gatcctggag aagtatctga aacagataac 2220 ggctctggtggtggcggcgg agtacctgaa agtgatcctc tttttgaaga agccaaggga 2280 ctcgttttagagacgcaaaa agcaagtgcc tcaatgattc aacgccgatt gtctgttggt 2340 ttcaatagagcaacaagact aatggaagaa ttagaagcag cgggggttat tggtccagca 2400 gaaggaaccaagccacgaaa agttttaatg actccaactc cgagtgaata a 2451 56 816 PRTStreptococcus agalactiae 56 Met Val Phe Met Ala Asn Lys Lys Lys Thr LysGly Lys Lys Thr Arg 1 5 10 15 Arg Pro Thr Lys Ala Glu Ile Glu Arg GlnArg Ala Ile Gln Arg Met 20 25 30 Ile Thr Ala Leu Val Leu Thr Ile Ile LeuPhe Phe Gly Ile Ile Arg 35 40 45 Leu Gly Ile Phe Gly Ile Thr Val Tyr AsnVal Ile Arg Phe Met Val 50 55 60 Gly Ser Leu Ala Tyr Leu Phe Ile Ala AlaThr Leu Ile Tyr Leu Tyr 65 70 75 80 Phe Phe Lys Trp Leu Arg Lys Lys AspSer Leu Val Ala Gly Phe Leu 85 90 95 Ile Ala Ser Leu Gly Leu Leu Ile GluTrp His Ala Tyr Leu Phe Ser 100 105 110 Met Pro Ile Leu Lys Asp Lys GluIle Leu Arg Ser Thr Ala Arg Leu 115 120 125 Ile Val Ser Asp Leu Met GlnPhe Lys Ile Thr Val Phe Ala Gly Gly 130 135 140 Gly Met Leu Gly Ala LeuIle Tyr Lys Pro Ile Ala Phe Leu Phe Ser 145 150 155 160 Asn Ile Gly AlaTyr Met Ile Gly Val Leu Phe Ile Ile Leu Gly Leu 165 170 175 Phe Leu MetSer Ser Leu Glu Val Tyr Asp Ile Val Glu Phe Ile Arg 180 185 190 Ala PheLys Asn Lys Val Ala Glu Lys His Glu Gln Asn Lys Lys Glu 195 200 205 ArgPhe Ala Lys Arg Glu Met Lys Lys Ala Ile Ala Glu Gln Glu Arg 210 215 220Ile Glu Arg Gln Lys Ala Glu Glu Glu Ala Tyr Leu Ala Ser Val Asn 225 230235 240 Val Asp Pro Glu Thr Gly Glu Ile Leu Glu Asp Gln Ala Glu Asp Asn245 250 255 Leu Asp Asp Ala Leu Pro Pro Glu Val Ser Glu Thr Ser Thr ProVal 260 265 270 Phe Glu Pro Glu Ile Leu Ala Tyr Glu Thr Ser Pro Gln AsnAsp Pro 275 280 285 Leu Pro Val Glu Pro Thr Ile Tyr Leu Glu Asp Tyr AspSer Pro Ile 290 295 300 Pro Asn Met Arg Glu Asn Asp Glu Glu Met Val TyrAsp Leu Asp Asp 305 310 315 320 Asp Val Asp Asp Ser Asp Ile Glu Asn ValAsp Phe Thr Pro Lys Thr 325 330 335 Thr Leu Val Tyr Lys Leu Pro Thr IleAsp Leu Phe Ala Pro Asp Lys 340 345 350 Pro Lys Asn Gln Ser Lys Glu LysAsp Leu Val Arg Lys Asn Ile Arg 355 360 365 Val Leu Glu Glu Thr Phe ArgSer Phe Gly Ile Asp Val Lys Val Glu 370 375 380 Arg Ala Glu Ile Gly ProSer Val Thr Lys Tyr Glu Ile Lys Pro Ala 385 390 395 400 Val Gly Val ArgVal Asn Arg Ile Ser Asn Leu Ser Asp Asp Leu Ala 405 410 415 Leu Ala LeuAla Ala Lys Asp Val Arg Ile Glu Ala Pro Ile Pro Gly 420 425 430 Lys SerLeu Ile Gly Ile Glu Val Pro Asn Ser Glu Ile Ala Thr Val 435 440 445 SerPhe Arg Glu Leu Trp Glu Gln Ser Asp Ala Asn Pro Glu Asn Leu 450 455 460Leu Glu Val Pro Leu Gly Lys Ala Val Asn Gly Asn Ala Arg Ser Phe 465 470475 480 Asn Leu Ala Arg Met Pro His Leu Leu Val Ala Gly Ser Thr Gly Ser485 490 495 Gly Lys Ser Val Ala Val Asn Gly Ile Ile Ser Ser Ile Leu MetLys 500 505 510 Ala Arg Pro Asp Gln Val Lys Phe Met Met Ile Asp Pro LysMet Val 515 520 525 Glu Leu Ser Val Tyr Asn Asp Ile Pro His Leu Leu IlePro Val Val 530 535 540 Thr Asn Pro Arg Lys Ala Ser Lys Ala Leu Gln LysVal Val Asp Glu 545 550 555 560 Met Glu Asn Arg Tyr Glu Leu Phe Ser LysIle Gly Val Arg Asn Ile 565 570 575 Ala Gly Tyr Asn Thr Lys Val Glu GluPhe Asn Ala Ser Ser Glu Gln 580 585 590 Lys Gln Met Pro Leu Pro Leu IleVal Val Ile Val Asp Glu Leu Ala 595 600 605 Asp Leu Met Met Val Ala SerLys Glu Val Glu Asp Ala Ile Ile Arg 610 615 620 Leu Gly Gln Lys Ala ArgAla Ala Gly Ile His Met Ile Leu Ala Thr 625 630 635 640 Gln Arg Pro SerVal Asp Val Ile Ser Gly Leu Ile Lys Ala Asn Val 645 650 655 Pro Ser ArgIle Ala Phe Ala Val Ser Ser Gly Thr Asp Ser Arg Thr 660 665 670 Ile LeuAsp Glu Asn Gly Ala Glu Lys Leu Leu Gly Arg Gly Asp Met 675 680 685 LeuPhe Lys Pro Ile Asp Glu Asn His Pro Val Arg Leu Gln Gly Ser 690 695 700Phe Ile Ser Asp Asp Asp Val Glu Arg Ile Val Gly Phe Ile Lys Asp 705 710715 720 Gln Ala Glu Ala Asp Tyr Asp Asp Ala Phe Asp Pro Gly Glu Val Ser725 730 735 Glu Thr Asp Asn Gly Ser Gly Gly Gly Gly Gly Val Pro Glu SerAsp 740 745 750 Pro Leu Phe Glu Glu Ala Lys Gly Leu Val Leu Glu Thr GlnLys Ala 755 760 765 Ser Ala Ser Met Ile Gln Arg Arg Leu Ser Val Gly PheAsn Arg Ala 770 775 780 Thr Arg Leu Met Glu Glu Leu Glu Ala Ala Gly ValIle Gly Pro Ala 785 790 795 800 Glu Gly Thr Lys Pro Arg Lys Val Leu MetThr Pro Thr Pro Ser Glu 805 810 815 57 669 DNA Streptococcus agalactiae57 atgtcacaag agcaaggaaa aatttatatt gtagaagatg atatgacgat tgtgtcactt 60ttaaaagatc atttatcagc tagctatcat gtctctagtg tcagcaattt tcgtgatgtg 120aaacaagaaa ttatcgcatt tcaacccgat ttgatactaa tggatattac gttaccctat 180tttaatggtt tttactggac tgcagaattg cgtaagtttt taacaattcc tattattttc 240atttcatcta gtaatgatga aatggatatg gttatggcat taaatatggg gggtgatgac 300tttatttcaa aaccattctc tctagctgta ttagatgcta agctaactgc tattttaagg 360agaagtcaac aatttatcca acaggaatta acttttgggg gatttacgtt gacaagagaa 420gggttattgt ctagccaaga taaagaggtt attttatcgc caacagaaaa taaaatccta 480tctatcttgc tcatgcatcc taaacaagta gtctcaaaag agtctctatt agagaaactt 540tgggaaaatg atagttttat tgatcaaaat acacttaatg ttaatatgac acgcttacgt 600aaaaaaattg tcccaatagg ttttgattac attcatacag tgagaggagt tgggtattta 660ctacaatga 669 58 222 PRT Streptococcus agalactiae 58 Met Ser Gln Glu GlnGly Lys Ile Tyr Ile Val Glu Asp Asp Met Thr 1 5 10 15 Ile Val Ser LeuLeu Lys Asp His Leu Ser Ala Ser Tyr His Val Ser 20 25 30 Ser Val Ser AsnPhe Arg Asp Val Lys Gln Glu Ile Ile Ala Phe Gln 35 40 45 Pro Asp Leu IleLeu Met Asp Ile Thr Leu Pro Tyr Phe Asn Gly Phe 50 55 60 Tyr Trp Thr AlaGlu Leu Arg Lys Phe Leu Thr Ile Pro Ile Ile Phe 65 70 75 80 Ile Ser SerSer Asn Asp Glu Met Asp Met Val Met Ala Leu Asn Met 85 90 95 Gly Gly AspAsp Phe Ile Ser Lys Pro Phe Ser Leu Ala Val Leu Asp 100 105 110 Ala LysLeu Thr Ala Ile Leu Arg Arg Ser Gln Gln Phe Ile Gln Gln 115 120 125 GluLeu Thr Phe Gly Gly Phe Thr Leu Thr Arg Glu Gly Leu Leu Ser 130 135 140Ser Gln Asp Lys Glu Val Ile Leu Ser Pro Thr Glu Asn Lys Ile Leu 145 150155 160 Ser Ile Leu Leu Met His Pro Lys Gln Val Val Ser Lys Glu Ser Leu165 170 175 Leu Glu Lys Leu Trp Glu Asn Asp Ser Phe Ile Asp Gln Asn ThrLeu 180 185 190 Asn Val Asn Met Thr Arg Leu Arg Lys Lys Ile Val Pro IleGly Phe 195 200 205 Asp Tyr Ile His Thr Val Arg Gly Val Gly Tyr Leu LeuGln 210 215 220 59 1341 DNA Streptococcus agalactiae 59 atgtatcaaactcagacaaa taaggaaaaa tttgttttat ttttgaaatt atttatccca 60 gtattgatttatcaatttgc taatttttca gctactttta ttgattcggt tatgactgga 120 cagtatagtcagctacattt ggcaggtgtg tcaactgcta gtaatttatg gactccgttt 180 ttcgctttattagtaggtat gatttcagca ttagtaccag tagttggtca acatttgggt 240 agaggaaataaagaacaaat tcgcacagaa tttcatcaat ttctatattt aggtttgata 300 ctgtccttaatattattttt aatcatgcaa tttattgctc aacctgtctt ggggagtttg 360 ggtttagaagatgaagttct agcagttggt cgtggttatt taaattatat gttgattgga 420 atcatgccgctggtgttgtt tagcatttgc cgttcattct ttgatgcatt ggggttaaca 480 aggttatctatgtatctgat gcttttaatt ctacccttta attcattttt taattatatg 540 cttatctacggtaaatttgg tatgcctaga ctaggaggtg cgggggcagg tcttggaact 600 tctttaacttattgggctat ttttattggt attattattg tgatgtcact tcatcctcaa 660 attaaaacatatcatatatg gactctggaa agaataaaag ctcctttgat tattgaagat 720 attcgattgggattaccgat tggtttacaa atttttgcag aagttgcaat ttttgcagta 780 gtaggcttattcatggcaaa attttcttca atcattattg cagcacatca ggctgctatg 840 aatttttcatcattaatgta tgcatttcct ttaagtattt ccactgctct agctattaca 900 atatcgtttgaagtaggggc agagcgcttt caggacgcaa ccacttatag taggatagga 960 cgcttaacagcggtagggat tacatcagga accttactat ttttatttct atttcgtgag 1020 aatgtagcagcaatgtataa tagtgcccct cactttgtcg ctattacagc tcaattccta 1080 acttatagtctctttttcca gtttgcagat gcttatgcag ctcctgtaca ggggatttta 1140 cgaggctataaggatacaac aaaaccattt atgatcggtg cgggctctta ttggttatgt 1200 gctttgccattagcggttat cttagaaaaa aatagccagt taggtccgtt tgcctattgg 1260 attggtttaatcacaggtat ttttgtttgt ggtctatttc taaaccaacg tctgcaaaag 1320 attaagaagttgtattatta a 1341 60 446 PRT Streptococcus agalactiae 60 Met Tyr Gln ThrGln Thr Asn Lys Glu Lys Phe Val Leu Phe Leu Lys 1 5 10 15 Leu Phe IlePro Val Leu Ile Tyr Gln Phe Ala Asn Phe Ser Ala Thr 20 25 30 Phe Ile AspSer Val Met Thr Gly Gln Tyr Ser Gln Leu His Leu Ala 35 40 45 Gly Val SerThr Ala Ser Asn Leu Trp Thr Pro Phe Phe Ala Leu Leu 50 55 60 Val Gly MetIle Ser Ala Leu Val Pro Val Val Gly Gln His Leu Gly 65 70 75 80 Arg GlyAsn Lys Glu Gln Ile Arg Thr Glu Phe His Gln Phe Leu Tyr 85 90 95 Leu GlyLeu Ile Leu Ser Leu Ile Leu Phe Leu Ile Met Gln Phe Ile 100 105 110 AlaGln Pro Val Leu Gly Ser Leu Gly Leu Glu Asp Glu Val Leu Ala 115 120 125Val Gly Arg Gly Tyr Leu Asn Tyr Met Leu Ile Gly Ile Met Pro Leu 130 135140 Val Leu Phe Ser Ile Cys Arg Ser Phe Phe Asp Ala Leu Gly Leu Thr 145150 155 160 Arg Leu Ser Met Tyr Leu Met Leu Leu Ile Leu Pro Phe Asn SerPhe 165 170 175 Phe Asn Tyr Met Leu Ile Tyr Gly Lys Phe Gly Met Pro ArgLeu Gly 180 185 190 Gly Ala Gly Ala Gly Leu Gly Thr Ser Leu Thr Tyr TrpAla Ile Phe 195 200 205 Ile Gly Ile Ile Ile Val Met Ser Leu His Pro GlnIle Lys Thr Tyr 210 215 220 His Ile Trp Thr Leu Glu Arg Ile Lys Ala ProLeu Ile Ile Glu Asp 225 230 235 240 Ile Arg Leu Gly Leu Pro Ile Gly LeuGln Ile Phe Ala Glu Val Ala 245 250 255 Ile Phe Ala Val Val Gly Leu PheMet Ala Lys Phe Ser Ser Ile Ile 260 265 270 Ile Ala Ala His Gln Ala AlaMet Asn Phe Ser Ser Leu Met Tyr Ala 275 280 285 Phe Pro Leu Ser Ile SerThr Ala Leu Ala Ile Thr Ile Ser Phe Glu 290 295 300 Val Gly Ala Glu ArgPhe Gln Asp Ala Thr Thr Tyr Ser Arg Ile Gly 305 310 315 320 Arg Leu ThrAla Val Gly Ile Thr Ser Gly Thr Leu Leu Phe Leu Phe 325 330 335 Leu PheArg Glu Asn Val Ala Ala Met Tyr Asn Ser Ala Pro His Phe 340 345 350 ValAla Ile Thr Ala Gln Phe Leu Thr Tyr Ser Leu Phe Phe Gln Phe 355 360 365Ala Asp Ala Tyr Ala Ala Pro Val Gln Gly Ile Leu Arg Gly Tyr Lys 370 375380 Asp Thr Thr Lys Pro Phe Met Ile Gly Ala Gly Ser Tyr Trp Leu Cys 385390 395 400 Ala Leu Pro Leu Ala Val Ile Leu Glu Lys Asn Ser Gln Leu GlyPro 405 410 415 Phe Ala Tyr Trp Ile Gly Leu Ile Thr Gly Ile Phe Val CysGly Leu 420 425 430 Phe Leu Asn Gln Arg Leu Gln Lys Ile Lys Lys Leu TyrTyr 435 440 445 61 1029 DNA Streptococcus agalactiae 61 ttgctagtttcttctctagt ttcttgttca ttttttcttg tcatttcgtc gttgtcttca 60 tcaacacgaaataagtctat aaacttatca aataatttca tagacttatt atatcaattt 120 tcaataaaatgctataataa aaccatgtca ttttcattaa aaattagaaa tccatacggt 180 gaacataccgttaaagaact ccttgaagat tattttttga ttccacgtaa gattagacat 240 tttttgcgtgttaaaaaaca tgtacttata aacaatgaat tcattaattg gcaaactgtc 300 gtccaagaaaacgatactat taccttaatc tttgatgatg aggattaccc tactaaaaaa 360 attcctctgggcagagcaga gcttattgat tgtctttatg aggatgaaca tcttattatc 420 gttaataaacctgaaggtat gaaaactcac ggtaaccaac caaatgaaat agcactgtta 480 aatcatgtatctgcctattc tggacaaaca tgctatgttg ttcatcgcct agatatggag 540 accagtggagctgttttatt tgctaaaaat ccatttatac ttccccttat caatcaacgc 600 ttagaacgaaaagaaatttg gcgtgaatat tgggctttag ttgaaggaaa attttcacct 660 aagcatcaagttttgagaga caaaattgga cggaaccgtc atgacagacg taaacgaatc 720 attgattctaaaaacggtca acatgctatg acaatcattg acgttttgaa gtatatccaa 780 aatagtagtctcataaaatg ccgactggaa accggaagaa cccatcaaat tcgcattcac 840 ttatctcatcacggacatcc tttaatagga gatcccctct acaacccttc ttctaataat 900 gaaaggttaatgctacacgc tcaccgattg actctatccc atccattaac ttgcgaaact 960 attagcgtagaggccccttc atctactttc gagaaggttt taaacaatta taaaaaagga 1020 gttggataa1029 62 342 PRT Streptococcus agalactiae 62 Met Leu Val Ser Ser Leu ValSer Cys Ser Phe Phe Leu Val Ile Ser 1 5 10 15 Ser Leu Ser Ser Ser ThrArg Asn Lys Ser Ile Asn Leu Ser Asn Asn 20 25 30 Phe Ile Asp Leu Leu TyrGln Phe Ser Ile Lys Cys Tyr Asn Lys Thr 35 40 45 Met Ser Phe Ser Leu LysIle Arg Asn Pro Tyr Gly Glu His Thr Val 50 55 60 Lys Glu Leu Leu Glu AspTyr Phe Leu Ile Pro Arg Lys Ile Arg His 65 70 75 80 Phe Leu Arg Val LysLys His Val Leu Ile Asn Asn Glu Phe Ile Asn 85 90 95 Trp Gln Thr Val ValGln Glu Asn Asp Thr Ile Thr Leu Ile Phe Asp 100 105 110 Asp Glu Asp TyrPro Thr Lys Lys Ile Pro Leu Gly Arg Ala Glu Leu 115 120 125 Ile Asp CysLeu Tyr Glu Asp Glu His Leu Ile Ile Val Asn Lys Pro 130 135 140 Glu GlyMet Lys Thr His Gly Asn Gln Pro Asn Glu Ile Ala Leu Leu 145 150 155 160Asn His Val Ser Ala Tyr Ser Gly Gln Thr Cys Tyr Val Val His Arg 165 170175 Leu Asp Met Glu Thr Ser Gly Ala Val Leu Phe Ala Lys Asn Pro Phe 180185 190 Ile Leu Pro Leu Ile Asn Gln Arg Leu Glu Arg Lys Glu Ile Trp Arg195 200 205 Glu Tyr Trp Ala Leu Val Glu Gly Lys Phe Ser Pro Lys His GlnVal 210 215 220 Leu Arg Asp Lys Ile Gly Arg Asn Arg His Asp Arg Arg LysArg Ile 225 230 235 240 Ile Asp Ser Lys Asn Gly Gln His Ala Met Thr IleIle Asp Val Leu 245 250 255 Lys Tyr Ile Gln Asn Ser Ser Leu Ile Lys CysArg Leu Glu Thr Gly 260 265 270 Arg Thr His Gln Ile Arg Ile His Leu SerHis His Gly His Pro Leu 275 280 285 Ile Gly Asp Pro Leu Tyr Asn Pro SerSer Asn Asn Glu Arg Leu Met 290 295 300 Leu His Ala His Arg Leu Thr LeuSer His Pro Leu Thr Cys Glu Thr 305 310 315 320 Ile Ser Val Glu Ala ProSer Ser Thr Phe Glu Lys Val Leu Asn Asn 325 330 335 Tyr Lys Lys Gly ValGly 340 63 2052 DNA Streptococcus agalactiae 63 gaactaaatg caactcaacctaataataga actacctata ttatacccga aagcagtcat 60 tccattgcag aacaacagagattcctgata gaatcaaagg gttcttcggt tgcattactt 120 aatagcgatg aatttagaaagacagcggga gaggatagag gttttgaaag ggataagttg 180 aggtctttgg atatcattcctaagggagat ttatcgacaa gtaatgtcat aggtaatacg 240 gacattgcta gtcagatatcgttgggcttt aaaaagaatg cgatgcagga acaccatctt 300 actaaaacat tctctcaaaaggatggaaag ttatcgtctg ttatagaggg gatgcttgct 360 attggcaaag agaaagtagagaaagaaata aaatatagtg gtaatttatg gcaaaaatta 420 aaagctaagg cacactgccttgtttgctgt gttgataatt tgaattttga agatataaaa 480 tcttattttc aatattattgtcatctaaac catcagctca aattacctaa aggtgctata 540 ctttctgcta aaacagaagtatatagggga ggagattttg ggagaaaaaa taaagataat 600 gtgtttggtt accgtatcccctcattattg aaaacccaaa aaggaacttt acttgcggga 660 gctgatgaaa gaattgagcaagcttgtgat tggggaaaca taggaatggt tattcgccgt 720 agtgaggatg atggtgtcacttggggaaaa agagaaacta ttgtcaatct ccgtaataac 780 cctagagttc cgctagttactagtggtgac tatagtggct cacctattaa tatggatatg 840 gcattagttc aagatactagctccaagacg aaacgtattt tttcaatata tgatatgttt 900 ccagaaggaa gaggcgttattagtattgct aacacacctg aaaaagaata tacccaaatc 960 ggaggacagt cttatcttaatttatataat aatggaaaga aatcgaaggt ttttactatc 1020 cgtgacaaag gtattgtatataattttaaa gggaaaaaga ctgattatca tgttataaca 1080 gaaactacta aaagtgaccattcaaatcta ggggatattt ataagggaaa acagctactt 1140 ggaaatatat attttacaaaacataaaacg tcaccatttc gtttagcaaa atcaagctat 1200 gtgtggatgt catatagcgatgatgatggt aggacatggt catcacctag agatataaca 1260 gcaagtcttc gtcagaaaggcatgaaattt ttgggaatag gacctggaaa aggtatagtt 1320 ttaaaatggg ggccacacgctggtcgtatt attattcctg cctattctac gaattggaaa 1380 tctcatctaa gaggttcacaatcttcacgc ctaatttatt cagacgacca tggaaaaacg 1440 tggcatactg gaaaagcagttaatgataac cgtatacttt ctaatggtga aaaaattcac 1500 tccttaacaa tggataataaaaaagaacaa aatacagaat ccgtacccgt tcaattgaaa 1560 aatggggaca ttaagttatttatgaggaat ctaactggta acctagaagt agccacaagt 1620 aaagacggcg gggagacttggcaaaaccat gttaaacgat ataaggaaat tcatgatgct 1680 tacgtccaac tatcagctattcgctttgag catgacaaaa aagagtatat tttattagtg 1740 aatgctaatg ggccagggaagaagtgccaa gatggatatg cacgtctagc gcaagttaat 1800 cgaaatggta gttttaagtggttatatcac catcacattc aagatggttc gtttgcttac 1860 aactctgttc aacaacttaataatgatcaa tttggtgtcc tttatgaaca tagagaaaaa 1920 catcaaaata gttttactttaaattacaaa gtttttaatt ggagttttct tagtcaaaat 1980 acagagaagc aaggcactttatgggagaaa atggcagcaa attggcatgt tttgtttaaa 2040 ttttatttat ga 2052 64683 PRT Streptococcus agalactiae 64 Glu Leu Asn Ala Thr Gln Pro Asn AsnArg Thr Thr Tyr Ile Ile Pro 1 5 10 15 Glu Ser Ser His Ser Ile Ala GluGln Gln Arg Phe Leu Ile Glu Ser 20 25 30 Lys Gly Ser Ser Val Ala Leu LeuAsn Ser Asp Glu Phe Arg Lys Thr 35 40 45 Ala Gly Glu Asp Arg Gly Phe GluArg Asp Lys Leu Arg Ser Leu Asp 50 55 60 Ile Ile Pro Lys Gly Asp Leu SerThr Ser Asn Val Ile Gly Asn Thr 65 70 75 80 Asp Ile Ala Ser Gln Ile SerLeu Gly Phe Lys Lys Asn Ala Met Gln 85 90 95 Glu His His Leu Thr Lys ThrPhe Ser Gln Lys Asp Gly Lys Leu Ser 100 105 110 Ser Val Ile Glu Gly MetLeu Ala Ile Gly Lys Glu Lys Val Glu Lys 115 120 125 Glu Ile Lys Tyr SerGly Asn Leu Trp Gln Lys Leu Lys Ala Lys Ala 130 135 140 His Cys Leu ValCys Cys Val Asp Asn Leu Asn Phe Glu Asp Ile Lys 145 150 155 160 Ser TyrPhe Gln Tyr Tyr Cys His Leu Asn His Gln Leu Lys Leu Pro 165 170 175 LysGly Ala Ile Leu Ser Ala Lys Thr Glu Val Tyr Arg Gly Gly Asp 180 185 190Phe Gly Arg Lys Asn Lys Asp Asn Val Phe Gly Tyr Arg Ile Pro Ser 195 200205 Leu Leu Lys Thr Gln Lys Gly Thr Leu Leu Ala Gly Ala Asp Glu Arg 210215 220 Ile Glu Gln Ala Cys Asp Trp Gly Asn Ile Gly Met Val Ile Arg Arg225 230 235 240 Ser Glu Asp Asp Gly Val Thr Trp Gly Lys Arg Glu Thr IleVal Asn 245 250 255 Leu Arg Asn Asn Pro Arg Val Pro Leu Val Thr Ser GlyAsp Tyr Ser 260 265 270 Gly Ser Pro Ile Asn Met Asp Met Ala Leu Val GlnAsp Thr Ser Ser 275 280 285 Lys Thr Lys Arg Ile Phe Ser Ile Tyr Asp MetPhe Pro Glu Gly Arg 290 295 300 Gly Val Ile Ser Ile Ala Asn Thr Pro GluLys Glu Tyr Thr Gln Ile 305 310 315 320 Gly Gly Gln Ser Tyr Leu Asn LeuTyr Asn Asn Gly Lys Lys Ser Lys 325 330 335 Val Phe Thr Ile Arg Asp LysGly Ile Val Tyr Asn Phe Lys Gly Lys 340 345 350 Lys Thr Asp Tyr His ValIle Thr Glu Thr Thr Lys Ser Asp His Ser 355 360 365 Asn Leu Gly Asp IleTyr Lys Gly Lys Gln Leu Leu Gly Asn Ile Tyr 370 375 380 Phe Thr Lys HisLys Thr Ser Pro Phe Arg Leu Ala Lys Ser Ser Tyr 385 390 395 400 Val TrpMet Ser Tyr Ser Asp Asp Asp Gly Arg Thr Trp Ser Ser Pro 405 410 415 ArgAsp Ile Thr Ala Ser Leu Arg Gln Lys Gly Met Lys Phe Leu Gly 420 425 430Ile Gly Pro Gly Lys Gly Ile Val Leu Lys Trp Gly Pro His Ala Gly 435 440445 Arg Ile Ile Ile Pro Ala Tyr Ser Thr Asn Trp Lys Ser His Leu Arg 450455 460 Gly Ser Gln Ser Ser Arg Leu Ile Tyr Ser Asp Asp His Gly Lys Thr465 470 475 480 Trp His Thr Gly Lys Ala Val Asn Asp Asn Arg Ile Leu SerAsn Gly 485 490 495 Glu Lys Ile His Ser Leu Thr Met Asp Asn Lys Lys GluGln Asn Thr 500 505 510 Glu Ser Val Pro Val Gln Leu Lys Asn Gly Asp IleLys Leu Phe Met 515 520 525 Arg Asn Leu Thr Gly Asn Leu Glu Val Ala ThrSer Lys Asp Gly Gly 530 535 540 Glu Thr Trp Gln Asn His Val Lys Arg TyrLys Glu Ile His Asp Ala 545 550 555 560 Tyr Val Gln Leu Ser Ala Ile ArgPhe Glu His Asp Lys Lys Glu Tyr 565 570 575 Ile Leu Leu Val Asn Ala AsnGly Pro Gly Lys Lys Cys Gln Asp Gly 580 585 590 Tyr Ala Arg Leu Ala GlnVal Asn Arg Asn Gly Ser Phe Lys Trp Leu 595 600 605 Tyr His His His IleGln Asp Gly Ser Phe Ala Tyr Asn Ser Val Gln 610 615 620 Gln Leu Asn AsnAsp Gln Phe Gly Val Leu Tyr Glu His Arg Glu Lys 625 630 635 640 His GlnAsn Ser Phe Thr Leu Asn Tyr Lys Val Phe Asn Trp Ser Phe 645 650 655 LeuSer Gln Asn Thr Glu Lys Gln Gly Thr Leu Trp Glu Lys Met Ala 660 665 670Ala Asn Trp His Val Leu Phe Lys Phe Tyr Leu 675 680 65 1188 DNAStreptococcus agalactiae 65 atgcctaaat taatcgtatc tttcctctgc attttattatccctgacttg tgtaaactct 60 gtgcaagctg aagaacataa agatattatg caaattacccgagaagccgg atatgatgtt 120 aaagatatta ataaacctaa agcgtctatc gttattgacaataaaggtca tattttgtgg 180 gaagataacg ccgatttaga acgtgatccc gctagcatgtctaaaatgtt tactttatat 240 ttactatttg aagacttagc taaaggaaaa acaaacctcaacaccacagt gactgcaaca 300 gaaacagacc aagccataag taagatttat gaaattagtaataacaatat tcatgctggg 360 gttgcttatc ctattcgtga actgattact atgacggctgtcccgtcatc taatgtagca 420 actattatga ttgctaacca cttatcacaa aacaatcctgacgcctttat taaacgaatc 480 aatgaaaccg ccaagaaact cggtatgaca aaaactcacttttataaccc cagtggggcg 540 gtagcgagtg cttttaatgg actttactcc ccaaaagaatacgataacaa tgctactaac 600 gttacgactg cacgtgatct atcaatttta acctatcatttccttaaaaa ataccctgat 660 atactgaact atacaaaata tcctgaagtc aaggccatggtcggaactcc ttatgaagaa 720 acatttacaa cttataacta ctctaccccc ggcgctaaatttggattaga aggagtagat 780 ggcttaaaaa ctggttctag ccctagcgct gcttttaatgccttagttac agctaaacgc 840 cagaatactc gcttgataac tgtggtttta ggagttggcgattggtcaga ccaagacgga 900 gagtactatc gtcatccgtt tgtcaacgct cttgtagaaaaaggttttaa agacgctaaa 960 aatatttctt ctaaaactcc tgtattaaaa gccgttaaacctaaaaaaga agttactaaa 1020 accaaaacta aatctattca agaacagcct caaacaaaagaacagtggtg gacaaaaaca 1080 gatcaattta tccaatcaca ttttgtatct attttaattgttctgggcac catcgctagc 1140 ctttgtcttt tagctgggat agtattactt ataaagcgctctagataa 1188 66 395 PRT Streptococcus agalactiae 66 Met Pro Lys Leu IleVal Ser Phe Leu Cys Ile Leu Leu Ser Leu Thr 1 5 10 15 Cys Val Asn SerVal Gln Ala Glu Glu His Lys Asp Ile Met Gln Ile 20 25 30 Thr Arg Glu AlaGly Tyr Asp Val Lys Asp Ile Asn Lys Pro Lys Ala 35 40 45 Ser Ile Val IleAsp Asn Lys Gly His Ile Leu Trp Glu Asp Asn Ala 50 55 60 Asp Leu Glu ArgAsp Pro Ala Ser Met Ser Lys Met Phe Thr Leu Tyr 65 70 75 80 Leu Leu PheGlu Asp Leu Ala Lys Gly Lys Thr Asn Leu Asn Thr Thr 85 90 95 Val Thr AlaThr Glu Thr Asp Gln Ala Ile Ser Lys Ile Tyr Glu Ile 100 105 110 Ser AsnAsn Asn Ile His Ala Gly Val Ala Tyr Pro Ile Arg Glu Leu 115 120 125 IleThr Met Thr Ala Val Pro Ser Ser Asn Val Ala Thr Ile Met Ile 130 135 140Ala Asn His Leu Ser Gln Asn Asn Pro Asp Ala Phe Ile Lys Arg Ile 145 150155 160 Asn Glu Thr Ala Lys Lys Leu Gly Met Thr Lys Thr His Phe Tyr Asn165 170 175 Pro Ser Gly Ala Val Ala Ser Ala Phe Asn Gly Leu Tyr Ser ProLys 180 185 190 Glu Tyr Asp Asn Asn Ala Thr Asn Val Thr Thr Ala Arg AspLeu Ser 195 200 205 Ile Leu Thr Tyr His Phe Leu Lys Lys Tyr Pro Asp IleLeu Asn Tyr 210 215 220 Thr Lys Tyr Pro Glu Val Lys Ala Met Val Gly ThrPro Tyr Glu Glu 225 230 235 240 Thr Phe Thr Thr Tyr Asn Tyr Ser Thr ProGly Ala Lys Phe Gly Leu 245 250 255 Glu Gly Val Asp Gly Leu Lys Thr GlySer Ser Pro Ser Ala Ala Phe 260 265 270 Asn Ala Leu Val Thr Ala Lys ArgGln Asn Thr Arg Leu Ile Thr Val 275 280 285 Val Leu Gly Val Gly Asp TrpSer Asp Gln Asp Gly Glu Tyr Tyr Arg 290 295 300 His Pro Phe Val Asn AlaLeu Val Glu Lys Gly Phe Lys Asp Ala Lys 305 310 315 320 Asn Ile Ser SerLys Thr Pro Val Leu Lys Ala Val Lys Pro Lys Lys 325 330 335 Glu Val ThrLys Thr Lys Thr Lys Ser Ile Gln Glu Gln Pro Gln Thr 340 345 350 Lys GluGln Trp Trp Thr Lys Thr Asp Gln Phe Ile Gln Ser His Phe 355 360 365 ValSer Ile Leu Ile Val Leu Gly Thr Ile Ala Ser Leu Cys Leu Leu 370 375 380Ala Gly Ile Val Leu Leu Ile Lys Arg Ser Arg 385 390 395 67 984 DNAStreptococcus agalactiae 67 atgactgaaa aatattataa ttgggcaacg cttggaaccggcgttattgc caacgaatta 60 gcccaagcac tggaagcacg tggacaaaaa ttatattctgtagctaatag aacttacgac 120 aaaggacttg aatttgctaa caaatatggt atccaaaaagtttatgatca catagatcaa 180 gtatttgaag accctgaagt ggatatcatt tatatctctactccccacaa tactcacatc 240 tcatttttac gaaaggcttt agcaaatggt aagcacgttctttgcgaaaa atctattact 300 ttaaatagta ctgagcttaa agaagccata gatttagccgaaactaacca tgttgtctta 360 gctgaagcca tgactatttt tcatatgcca atttaccgccaattaaaaac attagttgat 420 agtggaaaat taggaccgtt aaaaatgatt caaatgaatttcggaagtta taaagaatat 480 gatatgacta accgtttttt cagtcgtgac ctagcaggcggtgctttgct ggacattggt 540 gtttatgcac tttcttgtat tcgctggttt atgtcagaagcacctcacaa cattacctct 600 caagttacat ttgcaccaac aggggttgat gaacaagttggtatcctact aaccaaccca 660 gcaaatgaga tggcgactgt cagccttagt ttacatgcaaaacaacctaa acgagcaact 720 atcgcttacg ataaaggcta cattgaactt tttgaatatccgcgaggaca aaaggcagtt 780 attacttata ctgaggatgg gcatcaagat attatcgaagctggcaaaac tgaaaatgct 840 ctccaatatg aggtagctga tatggaagaa gccatttcaggaaaaactaa ccacatgtac 900 ttaaactata ccaaagatgt tatggatatc atgacacagctacgtcaaga atggggattt 960 acctacccag aagaagaaaa atga 984 68 327 PRTStreptococcus agalactiae 68 Met Thr Glu Lys Tyr Tyr Asn Trp Ala Thr LeuGly Thr Gly Val Ile 1 5 10 15 Ala Asn Glu Leu Ala Gln Ala Leu Glu AlaArg Gly Gln Lys Leu Tyr 20 25 30 Ser Val Ala Asn Arg Thr Tyr Asp Lys GlyLeu Glu Phe Ala Asn Lys 35 40 45 Tyr Gly Ile Gln Lys Val Tyr Asp His IleAsp Gln Val Phe Glu Asp 50 55 60 Pro Glu Val Asp Ile Ile Tyr Ile Ser ThrPro His Asn Thr His Ile 65 70 75 80 Ser Phe Leu Arg Lys Ala Leu Ala AsnGly Lys His Val Leu Cys Glu 85 90 95 Lys Ser Ile Thr Leu Asn Ser Thr GluLeu Lys Glu Ala Ile Asp Leu 100 105 110 Ala Glu Thr Asn His Val Val LeuAla Glu Ala Met Thr Ile Phe His 115 120 125 Met Pro Ile Tyr Arg Gln LeuLys Thr Leu Val Asp Ser Gly Lys Leu 130 135 140 Gly Pro Leu Lys Met IleGln Met Asn Phe Gly Ser Tyr Lys Glu Tyr 145 150 155 160 Asp Met Thr AsnArg Phe Phe Ser Arg Asp Leu Ala Gly Gly Ala Leu 165 170 175 Leu Asp IleGly Val Tyr Ala Leu Ser Cys Ile Arg Trp Phe Met Ser 180 185 190 Glu AlaPro His Asn Ile Thr Ser Gln Val Thr Phe Ala Pro Thr Gly 195 200 205 ValAsp Glu Gln Val Gly Ile Leu Leu Thr Asn Pro Ala Asn Glu Met 210 215 220Ala Thr Val Ser Leu Ser Leu His Ala Lys Gln Pro Lys Arg Ala Thr 225 230235 240 Ile Ala Tyr Asp Lys Gly Tyr Ile Glu Leu Phe Glu Tyr Pro Arg Gly245 250 255 Gln Lys Ala Val Ile Thr Tyr Thr Glu Asp Gly His Gln Asp IleIle 260 265 270 Glu Ala Gly Lys Thr Glu Asn Ala Leu Gln Tyr Glu Val AlaAsp Met 275 280 285 Glu Glu Ala Ile Ser Gly Lys Thr Asn His Met Tyr LeuAsn Tyr Thr 290 295 300 Lys Asp Val Met Asp Ile Met Thr Gln Leu Arg GlnGlu Trp Gly Phe 305 310 315 320 Thr Tyr Pro Glu Glu Glu Lys 325 69 96DNA Streptococcus agalactiae 69 gtgtattctc ctgttaaatc ttctaaaggaaaagtgatat tgttaaaaag tgattttcta 60 aagagcttca tagaaaggag aggaaatatttgtttt 96 70 32 PRT Streptococcus agalactiae 70 Met Tyr Ser Pro Val LysSer Ser Lys Gly Lys Val Ile Leu Leu Lys 1 5 10 15 Ser Asp Phe Leu LysSer Phe Ile Glu Arg Arg Gly Asn Ile Cys Phe 20 25 30 71 429 DNAStreptococcus agalactiae 71 aaatactgta tcattgcaac ctcaaatgca ggttttggaaacgaagcatt tacaggtgac 60 agcgataaag acttgaaaat tatggaacga atttctccatatttccgtcc agaatttcta 120 aatcgtttca atggtgttat tgaattctct cacctaagcaaagatgactt aagcgaaatt 180 gtagatttga tgcttgatga agttaaccaa acaattggcaaaaaaggaat tgaccttgtg 240 gtagatgaaa atgttaaatc acacttaatt gaactgggttatgacgaagc aatgggagta 300 cgtccattgc gccgtgtcat cgagcaagaa attcgagatcgcatcacaga ctactatctc 360 gatcatacag acgttaaaca cctaaaagct aatttgcaagatggccaaat cgtcatttct 420 gaaagataa 429 72 142 PRT Streptococcusagalactiae 72 Lys Tyr Cys Ile Ile Ala Thr Ser Asn Ala Gly Phe Gly AsnGlu Ala 1 5 10 15 Phe Thr Gly Asp Ser Asp Lys Asp Leu Lys Ile Met GluArg Ile Ser 20 25 30 Pro Tyr Phe Arg Pro Glu Phe Leu Asn Arg Phe Asn GlyVal Ile Glu 35 40 45 Phe Ser His Leu Ser Lys Asp Asp Leu Ser Glu Ile ValAsp Leu Met 50 55 60 Leu Asp Glu Val Asn Gln Thr Ile Gly Lys Lys Gly IleAsp Leu Val 65 70 75 80 Val Asp Glu Asn Val Lys Ser His Leu Ile Glu LeuGly Tyr Asp Glu 85 90 95 Ala Met Gly Val Arg Pro Leu Arg Arg Val Ile GluGln Glu Ile Arg 100 105 110 Asp Arg Ile Thr Asp Tyr Tyr Leu Asp His ThrAsp Val Lys His Leu 115 120 125 Lys Ala Asn Leu Gln Asp Gly Gln Ile ValIle Ser Glu Arg 130 135 140 73 699 DNA Streptococcus agalactiae 73atgtcaatga atttttcatt tttaccacaa tattggtcct attttaatta tggtgtgatg 60gtaaccatta tgatttcaac atgtgttgtt ttttttggaa ctattatagg cgtgttaatt 120gctttagtaa agcgtactaa tttacatttt ctcacaatat tagctaattt ctatgtatgg 180gtatttcgtg ggacaccgat ggtagttcaa attatgattg ctttcgcatg gatgcatttt 240aacaatttac caacaattag ctttggtgtt ttagatttag attttacacg acttttacct 300ggtatcatta tcatttcctt aaatagtggt gcctatattt cggaaattgt acgtgcaggg 360attgaggctg taccatctgg acaaatagaa gcagcttact cgttggggat tcgacctaaa 420aatacacttc gctatgttat cttaccccaa gcttttaaaa atattttacc tgctctaggg 480aatgaattta ttacaattat taaagatagt gctctccttc aaactattgg tgtcatggaa 540ttatggaacg gagcacaatc agttgtaacg gctacttact caccagttgc accgttatta 600tttgcagcat tttactattt aatgttgaca acgattctct cagctttgtt aaaacaaatg 660gagaaatatc ttgggaaagg ggtaaaaata gatggttga 699 74 232 PRT Streptococcusagalactiae 74 Met Ser Met Asn Phe Ser Phe Leu Pro Gln Tyr Trp Ser TyrPhe Asn 1 5 10 15 Tyr Gly Val Met Val Thr Ile Met Ile Ser Thr Cys ValVal Phe Phe 20 25 30 Gly Thr Ile Ile Gly Val Leu Ile Ala Leu Val Lys ArgThr Asn Leu 35 40 45 His Phe Leu Thr Ile Leu Ala Asn Phe Tyr Val Trp ValPhe Arg Gly 50 55 60 Thr Pro Met Val Val Gln Ile Met Ile Ala Phe Ala TrpMet His Phe 65 70 75 80 Asn Asn Leu Pro Thr Ile Ser Phe Gly Val Leu AspLeu Asp Phe Thr 85 90 95 Arg Leu Leu Pro Gly Ile Ile Ile Ile Ser Leu AsnSer Gly Ala Tyr 100 105 110 Ile Ser Glu Ile Val Arg Ala Gly Ile Glu AlaVal Pro Ser Gly Gln 115 120 125 Ile Glu Ala Ala Tyr Ser Leu Gly Ile ArgPro Lys Asn Thr Leu Arg 130 135 140 Tyr Val Ile Leu Pro Gln Ala Phe LysAsn Ile Leu Pro Ala Leu Gly 145 150 155 160 Asn Glu Phe Ile Thr Ile IleLys Asp Ser Ala Leu Leu Gln Thr Ile 165 170 175 Gly Val Met Glu Leu TrpAsn Gly Ala Gln Ser Val Val Thr Ala Thr 180 185 190 Tyr Ser Pro Val AlaPro Leu Leu Phe Ala Ala Phe Tyr Tyr Leu Met 195 200 205 Leu Thr Thr IleLeu Ser Ala Leu Leu Lys Gln Met Glu Lys Tyr Leu 210 215 220 Gly Lys GlyVal Lys Ile Asp Gly 225 230 75 678 DNA Streptococcus agalactiae 75atgaaagacc tattacgaaa tagtctagag caaagtggaa atttaagttt tcaagatatg 60attttacata ttcttgtagc agctttattg agtgtagtta tttatgtttc ctatgcttat 120acgcatagtg gaactgccta tagtaaaaag tttaatgttt cattaatgac attgacggtc 180ttgactgcaa cagtaatgac cgttattggt aataatgtag ccttgtcatt gggtatggtc 240ggtgccttgt cagttgttcg ttttaggaca gccataaaag attcaagaga tacagtttat 300attttttgga ccatagttgt tggtatctgt tgtggtgtcg gtgactatgt ggtagctgca 360ttaggaagta gcgttatctt tatcttatta tgggttatgg gacgtgttaa aaacgagaat 420cgtatgttat tgattgtgaa gtgcgataga acactagaag ttgatttaga aggaattttc 480ttccaatatt ttgacggaaa agctgttcag cgtgttaaaa attcaacaac taatactatt 540gaaatgattt tcgaaatctc tagaaaagat tacgataagc aactccatgt agataatcag 600ttaactgaaa aagtgtacca attgggaaat attgattatt tcaacattgt tagccaaagc 660gacgaaatca atgggtag 678 76 225 PRT Streptococcus agalactiae 76 Met LysAsp Leu Leu Arg Asn Ser Leu Glu Gln Ser Gly Asn Leu Ser 1 5 10 15 PheGln Asp Met Ile Leu His Ile Leu Val Ala Ala Leu Leu Ser Val 20 25 30 ValIle Tyr Val Ser Tyr Ala Tyr Thr His Ser Gly Thr Ala Tyr Ser 35 40 45 LysLys Phe Asn Val Ser Leu Met Thr Leu Thr Val Leu Thr Ala Thr 50 55 60 ValMet Thr Val Ile Gly Asn Asn Val Ala Leu Ser Leu Gly Met Val 65 70 75 80Gly Ala Leu Ser Val Val Arg Phe Arg Thr Ala Ile Lys Asp Ser Arg 85 90 95Asp Thr Val Tyr Ile Phe Trp Thr Ile Val Val Gly Ile Cys Cys Gly 100 105110 Val Gly Asp Tyr Val Val Ala Ala Leu Gly Ser Ser Val Ile Phe Ile 115120 125 Leu Leu Trp Val Met Gly Arg Val Lys Asn Glu Asn Arg Met Leu Leu130 135 140 Ile Val Lys Cys Asp Arg Thr Leu Glu Val Asp Leu Glu Gly IlePhe 145 150 155 160 Phe Gln Tyr Phe Asp Gly Lys Ala Val Gln Arg Val LysAsn Ser Thr 165 170 175 Thr Asn Thr Ile Glu Met Ile Phe Glu Ile Ser ArgLys Asp Tyr Asp 180 185 190 Lys Gln Leu His Val Asp Asn Gln Leu Thr GluLys Val Tyr Gln Leu 195 200 205 Gly Asn Ile Asp Tyr Phe Asn Ile Val SerGln Ser Asp Glu Ile Asn 210 215 220 Gly 225 77 499 DNA Streptococcusagalactiae 77 aaaaattcat tttagattca ttttacgact atatactcag aagtaccaaacctaatccaa 60 ggtttgaaaa aagaaagaag gaagtcagta tgacaaacta taaaaacaactttaaagatg 120 aggctatacg tgttgaagag acaacaaaag aatcatttta cgatgttgatattgccttgt 180 tttcagctgg tggatctatt tcagcaaagt tcgctcctta tgcagtaaagtctggagcag 240 ttgtagtaga taacacgtca tattttcgtc agaatcctga tgttccactagttgttcctg 300 aagtaaatgc tcatgccatg attggtcata atggtatcat agcttgtcccaattgttcta 360 ctattcaaat gatgattgct ttagagccca ttcgtcaaaa atgggggatagagcgtgtta 420 tagtttccac ctatcaagct gtttcgggtt caggtgcacg tgctgttgaagaaactaagg 480 aacagttgag acaagtttt 499 78 165 PRT Streptococcusagalactiae 78 Lys Phe Ile Leu Asp Ser Phe Tyr Asp Tyr Ile Leu Arg SerThr Lys 1 5 10 15 Pro Asn Pro Arg Phe Glu Lys Arg Lys Lys Glu Val SerMet Thr Asn 20 25 30 Tyr Lys Asn Asn Phe Lys Asp Glu Ala Ile Arg Val GluGlu Thr Thr 35 40 45 Lys Glu Ser Phe Tyr Asp Val Asp Ile Ala Leu Phe SerAla Gly Gly 50 55 60 Ser Ile Ser Ala Lys Phe Ala Pro Tyr Ala Val Lys SerGly Ala Val 65 70 75 80 Val Val Asp Asn Thr Ser Tyr Phe Arg Gln Asn ProAsp Val Pro Leu 85 90 95 Val Val Pro Glu Val Asn Ala His Ala Met Ile GlyHis Asn Gly Ile 100 105 110 Ile Ala Cys Pro Asn Cys Ser Thr Ile Gln MetMet Ile Ala Leu Glu 115 120 125 Pro Ile Arg Gln Lys Trp Gly Ile Glu ArgVal Ile Val Ser Thr Tyr 130 135 140 Gln Ala Val Ser Gly Ser Gly Ala ArgAla Val Glu Glu Thr Lys Glu 145 150 155 160 Gln Leu Arg Gln Val 165 79456 DNA Streptococcus agalactiae 79 atgacaaatg aattgataat gcaagcttttgagtggtatt tacctagtga tgggaatcac 60 tggaagaaat tagaggagtc tatatcagaccttaaaaaac ttggaattag taaaatctgg 120 ttaccaccag catttaaggg aactagcagtgatgatgtag gatatggtgt ttatgatctc 180 tttgatttag gagaatttga ccagaatggaacaattagaa caaaatatgg taggaaagaa 240 gagtatctaa agcttattaa gtcgttaaaggcaaatggca ttaaaccgtt tgcagatatc 300 gttcttaacc ataaagccaa tggtgatcataaagaaaaat ttcaagtcat caaagtcaat 360 cctgaaaatc gtcaagaagc attaagtgaaccctatgaga ttgaaggatg gacgggattt 420 gatttcccag gtagacaggg tgagtacaatgatttt 456 80 152 PRT Streptococcus agalactiae 80 Met Thr Asn Glu LeuIle Met Gln Ala Phe Glu Trp Tyr Leu Pro Ser 1 5 10 15 Asp Gly Asn HisTrp Lys Lys Leu Glu Glu Ser Ile Ser Asp Leu Lys 20 25 30 Lys Leu Gly IleSer Lys Ile Trp Leu Pro Pro Ala Phe Lys Gly Thr 35 40 45 Ser Ser Asp AspVal Gly Tyr Gly Val Tyr Asp Leu Phe Asp Leu Gly 50 55 60 Glu Phe Asp GlnAsn Gly Thr Ile Arg Thr Lys Tyr Gly Arg Lys Glu 65 70 75 80 Glu Tyr LeuLys Leu Ile Lys Ser Leu Lys Ala Asn Gly Ile Lys Pro 85 90 95 Phe Ala AspIle Val Leu Asn His Lys Ala Asn Gly Asp His Lys Glu 100 105 110 Lys PheGln Val Ile Lys Val Asn Pro Glu Asn Arg Gln Glu Ala Leu 115 120 125 SerGlu Pro Tyr Glu Ile Glu Gly Trp Thr Gly Phe Asp Phe Pro Gly 130 135 140Arg Gln Gly Glu Tyr Asn Asp Phe 145 150 81 516 DNA Streptococcusagalactiae 81 atggaggttc ttatgaagaa agtgttagta agtagtcttt tggttttagggattacgata 60 acgttacaac cagtagttga ggctaagggg ccaaaagtag cttatacacaagagggaatg 120 actgctcttt cggacacaaa taaagataaa gtcactacta tttctattgacgagattcaa 180 aaaagcttag aaggtaagaa gccgattact gttagttttg atattgatgatacactgctt 240 ttcagtagtc aatattttca atatggtaaa gaatatgtaa ctcctggatcgtttgatttt 300 cttcataaac aaaaattctg ggatcttgtt gcaaaacgag gagatcaagattccattccc 360 aaagaatatg ctaaaaaatt aattgctatg catcaaaaac gaggagataaaattgttttt 420 ataacaggta ggacaagagg gtcaatgtat aaggagggcg aggttgataaaacagctaaa 480 gccttagcta aagattttaa atttgtacca tctgat 516 82 172 PRTStreptococcus agalactiae 82 Met Glu Val Leu Met Lys Lys Val Leu Val SerSer Leu Leu Val Leu 1 5 10 15 Gly Ile Thr Ile Thr Leu Gln Pro Val ValGlu Ala Lys Gly Pro Lys 20 25 30 Val Ala Tyr Thr Gln Glu Gly Met Thr AlaLeu Ser Asp Thr Asn Lys 35 40 45 Asp Lys Val Thr Thr Ile Ser Ile Asp GluIle Gln Lys Ser Leu Glu 50 55 60 Gly Lys Lys Pro Ile Thr Val Ser Phe AspIle Asp Asp Thr Leu Leu 65 70 75 80 Phe Ser Ser Gln Tyr Phe Gln Tyr GlyLys Glu Tyr Val Thr Pro Gly 85 90 95 Ser Phe Asp Phe Leu His Lys Gln LysPhe Trp Asp Leu Val Ala Lys 100 105 110 Arg Gly Asp Gln Asp Ser Ile ProLys Glu Tyr Ala Lys Lys Leu Ile 115 120 125 Ala Met His Gln Lys Arg GlyAsp Lys Ile Val Phe Ile Thr Gly Arg 130 135 140 Thr Arg Gly Ser Met TyrLys Glu Gly Glu Val Asp Lys Thr Ala Lys 145 150 155 160 Ala Leu Ala LysAsp Phe Lys Phe Val Pro Ser Asp 165 170 83 516 DNA Streptococcusagalactiae 83 atgcttaaaa gattatttac tgaagatggg gaattgacaa agattagtcgtcgtttcgtt 60 tggatgttag tggttatcta ttgtcttatt attgtcagga tgtgttttgggcctcaaatt 120 atgattgagg gggtatcaac tccgaatgtt cagcgcttcg gaagaattgtagctctttta 180 gtaccattta attcttttcg tagtttagat cagctaacta gctttaaagagattctttgg 240 gttattggtc aaaatgtagt gaatatttta ctgctgtttc ctctcattatagggttacta 300 tccctaaagc caagtttacg gaaatataaa agcgttatat tacttgctttcttgatgtct 360 cttttcatag agtgtactca agttgtttta gatattttaa tagatgctaatcgggttttt 420 gaaatcgacg atctatggac aaatacctta ggcggtcctt tcgccctatggagttatcga 480 aacataaaag gttggcttct aactattaga aaatga 516 84 171 PRTStreptococcus agalactiae 84 Met Leu Lys Arg Leu Phe Thr Glu Asp Gly GluLeu Thr Lys Ile Ser 1 5 10 15 Arg Arg Phe Val Trp Met Leu Val Val IleTyr Cys Leu Ile Ile Val 20 25 30 Arg Met Cys Phe Gly Pro Gln Ile Met IleGlu Gly Val Ser Thr Pro 35 40 45 Asn Val Gln Arg Phe Gly Arg Ile Val AlaLeu Leu Val Pro Phe Asn 50 55 60 Ser Phe Arg Ser Leu Asp Gln Leu Thr SerPhe Lys Glu Ile Leu Trp 65 70 75 80 Val Ile Gly Gln Asn Val Val Asn IleLeu Leu Leu Phe Pro Leu Ile 85 90 95 Ile Gly Leu Leu Ser Leu Lys Pro SerLeu Arg Lys Tyr Lys Ser Val 100 105 110 Ile Leu Leu Ala Phe Leu Met SerLeu Phe Ile Glu Cys Thr Gln Val 115 120 125 Val Leu Asp Ile Leu Ile AspAla Asn Arg Val Phe Glu Ile Asp Asp 130 135 140 Leu Trp Thr Asn Thr LeuGly Gly Pro Phe Ala Leu Trp Ser Tyr Arg 145 150 155 160 Asn Ile Lys GlyTrp Leu Leu Thr Ile Arg Lys 165 170 85 627 DNA Streptococcus agalactiae85 atgaaaaagc ttacttttat ttgggattta gatgggacat taatagattc gtatgtacca 60attatggaag ctcttgaaga aacctatcgt cattttggct taatatttga taaagaatta 120atccatgaat atattttaca ggaatcagtg gggcaattat tggtaaacct ttcagaggaa 180gagcaaatac ctcatgaaaa actgaaagca tattttacaa aagaacaaga aagtcgagat 240tctaaaatac atttaatgcc atatgcaaaa gagattttag aatggaccaa agaacaagat 300attcccaatt ttatgtatac acataaagga gcaagtacgc attcagtgtt ggaaaccttg 360cagatctctc attattttga tgaaatttta actggtgttt cgggattcga gcgaaaacca 420catccacaag ggattaatta tttagttaaa cgatattctt tagataaatc aatgacttat 480tacataggag atcgtccact agatttggag gttgctcaaa atgctggtat aaaatccata 540aacttaaggt tagagaattc caaagaaaac tataatattt caagtctcaa agatataata 600tcacttgatt tcactcgttt ggattaa 627 86 208 PRT Streptococcus agalactiae 86Met Lys Lys Leu Thr Phe Ile Trp Asp Leu Asp Gly Thr Leu Ile Asp 1 5 1015 Ser Tyr Val Pro Ile Met Glu Ala Leu Glu Glu Thr Tyr Arg His Phe 20 2530 Gly Leu Ile Phe Asp Lys Glu Leu Ile His Glu Tyr Ile Leu Gln Glu 35 4045 Ser Val Gly Gln Leu Leu Val Asn Leu Ser Glu Glu Glu Gln Ile Pro 50 5560 His Glu Lys Leu Lys Ala Tyr Phe Thr Lys Glu Gln Glu Ser Arg Asp 65 7075 80 Ser Lys Ile His Leu Met Pro Tyr Ala Lys Glu Ile Leu Glu Trp Thr 8590 95 Lys Glu Gln Asp Ile Pro Asn Phe Met Tyr Thr His Lys Gly Ala Ser100 105 110 Thr His Ser Val Leu Glu Thr Leu Gln Ile Ser His Tyr Phe AspGlu 115 120 125 Ile Leu Thr Gly Val Ser Gly Phe Glu Arg Lys Pro His ProGln Gly 130 135 140 Ile Asn Tyr Leu Val Lys Arg Tyr Ser Leu Asp Lys SerMet Thr Tyr 145 150 155 160 Tyr Ile Gly Asp Arg Pro Leu Asp Leu Glu ValAla Gln Asn Ala Gly 165 170 175 Ile Lys Ser Ile Asn Leu Arg Leu Glu AsnSer Lys Glu Asn Tyr Asn 180 185 190 Ile Ser Ser Leu Lys Asp Ile Ile SerLeu Asp Phe Thr Arg Leu Asp 195 200 205 87 1356 DNA Streptococcusagalactiae 87 atggaaaaag aaaaaaaatt aggtctttta ccactaacaa tgcttgtcattggctctctt 60 atcggtggcg gaatctttga tttaatgcaa aatatgagtt ccagagccggtttggtacca 120 atgcttattg cttgggtaat tactgctatc gggatgggaa ctttcgttttaagttttcaa 180 aatttatctg aaaaaaggcc ggacctaaca gctggaatct ttagttacgctaaagagggg 240 tttggaaact ttatgggatt taactctgca tggggttatt ggttatcagcttggcttgga 300 aatgttgcct acgctgcact cttattcagt tcactcggtt atttctttaaattctttggt 360 aatggaaata atatcatctc aattattgga gcaagtatag ttatttgggttgtccatttc 420 ttaattttaa gaggtgttaa tacagctgca tttattaata ccgtagttacctttgcaaaa 480 ttagtacctg ttattatttt cttaatttca gcgttattag ctttcaaatttaacattttt 540 agtcttgata tctggggaaa tggattacat caatcaattt tcaaccaagtcaattcaact 600 atgaaaaccg ctgtttgggt atttattggt attgagggcg ccgttgtcttctcaggtcgt 660 gctaaaaaac actctgatat tggtaaagca agtatcctag cattattcactatgatttca 720 ctttatgtat tgatttctgt tttatcactt ggtatcatgt cacgtccagaacttgcaaac 780 ttaaaaacac cagctatggc ttacgttcta gaaaaagctg ttggtcactggggtgctatc 840 ttagttaacc ttggtgttat catttcagta tttggcgcta ttcttgcttggactttattt 900 gcagcagaat taccatatca agctgctaaa gaaggtgctt ttcctaaattttttgcaaaa 960 gaaaataaaa acaaagctcc aatcaactca ctcttagtca ctaatctttgtgtacaagca 1020 ttcttaatca cgttcttatt cacacaaagt gcttatcgtt ttggtttcgcattagcatca 1080 tctgctatct taattcctta tgcttttaca gcactatatc aattacaattcacactccgt 1140 gaggataagt caactccagg acatcaaaag aatttaatta tcggtatcctcgctacaatc 1200 tatgctgttt accttatcta cgctggtggt tttgattact tacttttgacaatgattgct 1260 tatactctag gtatgattct ctatattaaa atgagaaaag atgacaagcttggcgtaatc 1320 atggtcatag ctgtttccag tgtgaaattg ttatcc 1356 88 452 PRTStreptococcus agalactiae 88 Met Glu Lys Glu Lys Lys Leu Gly Leu Leu ProLeu Thr Met Leu Val 1 5 10 15 Ile Gly Ser Leu Ile Gly Gly Gly Ile PheAsp Leu Met Gln Asn Met 20 25 30 Ser Ser Arg Ala Gly Leu Val Pro Met LeuIle Ala Trp Val Ile Thr 35 40 45 Ala Ile Gly Met Gly Thr Phe Val Leu SerPhe Gln Asn Leu Ser Glu 50 55 60 Lys Arg Pro Asp Leu Thr Ala Gly Ile PheSer Tyr Ala Lys Glu Gly 65 70 75 80 Phe Gly Asn Phe Met Gly Phe Asn SerAla Trp Gly Tyr Trp Leu Ser 85 90 95 Ala Trp Leu Gly Asn Val Ala Tyr AlaAla Leu Leu Phe Ser Ser Leu 100 105 110 Gly Tyr Phe Phe Lys Phe Phe GlyAsn Gly Asn Asn Ile Ile Ser Ile 115 120 125 Ile Gly Ala Ser Ile Val IleTrp Val Val His Phe Leu Ile Leu Arg 130 135 140 Gly Val Asn Thr Ala AlaPhe Ile Asn Thr Val Val Thr Phe Ala Lys 145 150 155 160 Leu Val Pro ValIle Ile Phe Leu Ile Ser Ala Leu Leu Ala Phe Lys 165 170 175 Phe Asn IlePhe Ser Leu Asp Ile Trp Gly Asn Gly Leu His Gln Ser 180 185 190 Ile PheAsn Gln Val Asn Ser Thr Met Lys Thr Ala Val Trp Val Phe 195 200 205 IleGly Ile Glu Gly Ala Val Val Phe Ser Gly Arg Ala Lys Lys His 210 215 220Ser Asp Ile Gly Lys Ala Ser Ile Leu Ala Leu Phe Thr Met Ile Ser 225 230235 240 Leu Tyr Val Leu Ile Ser Val Leu Ser Leu Gly Ile Met Ser Arg Pro245 250 255 Glu Leu Ala Asn Leu Lys Thr Pro Ala Met Ala Tyr Val Leu GluLys 260 265 270 Ala Val Gly His Trp Gly Ala Ile Leu Val Asn Leu Gly ValIle Ile 275 280 285 Ser Val Phe Gly Ala Ile Leu Ala Trp Thr Leu Phe AlaAla Glu Leu 290 295 300 Pro Tyr Gln Ala Ala Lys Glu Gly Ala Phe Pro LysPhe Phe Ala Lys 305 310 315 320 Glu Asn Lys Asn Lys Ala Pro Ile Asn SerLeu Leu Val Thr Asn Leu 325 330 335 Cys Val Gln Ala Phe Leu Ile Thr PheLeu Phe Thr Gln Ser Ala Tyr 340 345 350 Arg Phe Gly Phe Ala Leu Ala SerSer Ala Ile Leu Ile Pro Tyr Ala 355 360 365 Phe Thr Ala Leu Tyr Gln LeuGln Phe Thr Leu Arg Glu Asp Lys Ser 370 375 380 Thr Pro Gly His Gln LysAsn Leu Ile Ile Gly Ile Leu Ala Thr Ile 385 390 395 400 Tyr Ala Val TyrLeu Ile Tyr Ala Gly Gly Phe Asp Tyr Leu Leu Leu 405 410 415 Thr Met IleAla Tyr Thr Leu Gly Met Ile Leu Tyr Ile Lys Met Arg 420 425 430 Lys AspAsp Lys Leu Gly Val Ile Met Val Ile Ala Val Ser Ser Val 435 440 445 LysLeu Leu Ser 450 89 1134 DNA Streptococcus agalactiae 89 atgaaatttgaaaaacggca ggtctattat gttgtcataa catttgctat ttgctatgct 60 atacaggcttattggggagc tgtttctaat attttaacta cgcttcataa ggcaatattt 120 ccttttttgatgggagctgg aattgcctat attattaata ttgtaatgtc agtctatgag 180 cgattatatataaagctttt taaaggatct agactattaa tggcaatcaa gcgtagtgtt 240 tctatgattttatcctatgc aacttttatt ggtttaattg tctggctatt ttcaattgtc 300 attccagatttgatttctag tttgagttct ttattggtta ttgataccgg agcacttgct 360 aaattggttaataatctcaa tgaaaataaa caaatttctg aggctttaaa ttatatggga 420 acagataaagacttagtttc tactttaagt ggttatagcc agcagatttt gaagcaagtt 480 ttatctgttttaacaaattt actaacctca gtttcctcta ttgcggcaac acttctgaat 540 gtttttgttagttttatttt ttcaatttac gttttggcaa acaaggagca gttgggacgt 600 caatttaatttgttaattga tacctattta ggttcaacag gcaaaacatt ccattacgtt 660 cgtcatatccttcatcaacg tttccatggt ttttttgtaa gccaaacttt agaagctatg 720 attttaggaagtttgacggt tattggtatg ttgatcttcc aatttcctta tgctttaaca 780 gttggggttttagttgcttt tacagctcta ataccggttg tgggagccta cattggtgtt 840 acaatcggtttcatcttaat tgctactgaa tcgcttactg aagcattctt gtttgttctt 900 ttcttgatccttttacaaca atttgaggga aatgtcattt atccgaaagt tgtcggtgga 960 tcgattggactgccttctat gtgggtttta atggctatta ctatcggagg tgctttatgg 1020 gggatcttaggcatgttact tgctgttcct gttgcagcta ctatctatca gattgtaaaa 1080 gatcatattatcaagcgaca aacgcttaga aatcgtgcac gaacctatcg ttaa 1134 90 377 PRTStreptococcus agalactiae 90 Met Lys Phe Glu Lys Arg Gln Val Tyr Tyr ValVal Ile Thr Phe Ala 1 5 10 15 Ile Cys Tyr Ala Ile Gln Ala Tyr Trp GlyAla Val Ser Asn Ile Leu 20 25 30 Thr Thr Leu His Lys Ala Ile Phe Pro PheLeu Met Gly Ala Gly Ile 35 40 45 Ala Tyr Ile Ile Asn Ile Val Met Ser ValTyr Glu Arg Leu Tyr Ile 50 55 60 Lys Leu Phe Lys Gly Ser Arg Leu Leu MetAla Ile Lys Arg Ser Val 65 70 75 80 Ser Met Ile Leu Ser Tyr Ala Thr PheIle Gly Leu Ile Val Trp Leu 85 90 95 Phe Ser Ile Val Ile Pro Asp Leu IleSer Ser Leu Ser Ser Leu Leu 100 105 110 Val Ile Asp Thr Gly Ala Leu AlaLys Leu Val Asn Asn Leu Asn Glu 115 120 125 Asn Lys Gln Ile Ser Glu AlaLeu Asn Tyr Met Gly Thr Asp Lys Asp 130 135 140 Leu Val Ser Thr Leu SerGly Tyr Ser Gln Gln Ile Leu Lys Gln Val 145 150 155 160 Leu Ser Val LeuThr Asn Leu Leu Thr Ser Val Ser Ser Ile Ala Ala 165 170 175 Thr Leu LeuAsn Val Phe Val Ser Phe Ile Phe Ser Ile Tyr Val Leu 180 185 190 Ala AsnLys Glu Gln Leu Gly Arg Gln Phe Asn Leu Leu Ile Asp Thr 195 200 205 TyrLeu Gly Ser Thr Gly Lys Thr Phe His Tyr Val Arg His Ile Leu 210 215 220His Gln Arg Phe His Gly Phe Phe Val Ser Gln Thr Leu Glu Ala Met 225 230235 240 Ile Leu Gly Ser Leu Thr Val Ile Gly Met Leu Ile Phe Gln Phe Pro245 250 255 Tyr Ala Leu Thr Val Gly Val Leu Val Ala Phe Thr Ala Leu IlePro 260 265 270 Val Val Gly Ala Tyr Ile Gly Val Thr Ile Gly Phe Ile LeuIle Ala 275 280 285 Thr Glu Ser Leu Thr Glu Ala Phe Leu Phe Val Leu PheLeu Ile Leu 290 295 300 Leu Gln Gln Phe Glu Gly Asn Val Ile Tyr Pro LysVal Val Gly Gly 305 310 315 320 Ser Ile Gly Leu Pro Ser Met Trp Val LeuMet Ala Ile Thr Ile Gly 325 330 335 Gly Ala Leu Trp Gly Ile Leu Gly MetLeu Leu Ala Val Pro Val Ala 340 345 350 Ala Thr Ile Tyr Gln Ile Val LysAsp His Ile Ile Lys Arg Gln Thr 355 360 365 Leu Arg Asn Arg Ala Arg ThrTyr Arg 370 375 91 1386 DNA Streptococcus agalactiae 91 gtgattacaattaaaaagga atctgttatc aaactattga agtatgcttt tggcattata 60 atgggatttattatcttagc tattgtaata ggtgggctcc tatttgcata ctacgttagt 120 cgttctccgaaattaaccga tcaagcttta aaatccgtta actctagttt ggtttatgat 180 ggtaataataaacttattgc cgatttaggc tcagaaaagc gtgaaagtgt tagtgcggat 240 agcattccactaaatttggt taacgctatc acttctatag aagataaacg tttctttaaa 300 catagaggtgtcgatattta tcgtatttta ggtgcagctt ggcataacct tgttagtagt 360 aatacgcaaggtggttcaac ccttgatcaa cagttgatta aactggctta cttttctacc 420 aataaatctgaccaaacgtt aaaacgtaaa tcacaggaag tttggcttgc gcttcaaatg 480 gagcgtaaatacaccaaaga agaaattctt actttctata ttaataaagt ttatatggga 540 aatgggaattatggtatgag aacaacagct aaatcatact ttggtaaaga cctaaaggaa 600 ttatctattgcacaacttgc tttgctcgct ggtattcctc aagcacctac acaatatgac 660 ccttataaaaacccagaatc tgctcaaaca agacgtaata ccgttcttca gcagatgtat 720 caagataaaaacatttctaa aaaggaatac gaccaagctg ttgcaactcc agtaactgat 780 ggcttaaaagaattaaagca aaaatctact tatccaaaat atatggataa ctacttaaaa 840 caagttattagtgaagttaa acaaaaaact ggtaaagata tctttactgc tgggctaaaa 900 gtgtatactaatatcaacac tgatgcacaa aaacaactat atgacatcta caacagtgat 960 acttacatcgcttatccaaa caatgaatta caaatagcat ctaccatcat ggatgcgact 1020 aatggtaaagtcattgcaca attaggcggg cgtcatcaga atgaaaatat ttcatttggg 1080 acaaatcaatctgtcttaac agaccgcgat tggggttcta caatgaaacc tatctcagct 1140 tatgcacctgctattgatag tggtgtctat aattcaacag gtcaatcatt aaacgactca 1200 gtttactactggcctggtac ttctactcaa ctatatgact gggatcgtca atatatgggt 1260 tggatgagtatgcagaccgc tattcaacaa tcacgtaacg tccctgctgt cagagcactt 1320 gaagccgctggattagacga agcaaaatct ttccttgaaa aattaggcat atactatcca 1380 gaaatg 138692 462 PRT Streptococcus agalactiae 92 Met Ile Thr Ile Lys Lys Glu SerVal Ile Lys Leu Leu Lys Tyr Ala 1 5 10 15 Phe Gly Ile Ile Met Gly PheIle Ile Leu Ala Ile Val Ile Gly Gly 20 25 30 Leu Leu Phe Ala Tyr Tyr ValSer Arg Ser Pro Lys Leu Thr Asp Gln 35 40 45 Ala Leu Lys Ser Val Asn SerSer Leu Val Tyr Asp Gly Asn Asn Lys 50 55 60 Leu Ile Ala Asp Leu Gly SerGlu Lys Arg Glu Ser Val Ser Ala Asp 65 70 75 80 Ser Ile Pro Leu Asn LeuVal Asn Ala Ile Thr Ser Ile Glu Asp Lys 85 90 95 Arg Phe Phe Lys His ArgGly Val Asp Ile Tyr Arg Ile Leu Gly Ala 100 105 110 Ala Trp His Asn LeuVal Ser Ser Asn Thr Gln Gly Gly Ser Thr Leu 115 120 125 Asp Gln Gln LeuIle Lys Leu Ala Tyr Phe Ser Thr Asn Lys Ser Asp 130 135 140 Gln Thr LeuLys Arg Lys Ser Gln Glu Val Trp Leu Ala Leu Gln Met 145 150 155 160 GluArg Lys Tyr Thr Lys Glu Glu Ile Leu Thr Phe Tyr Ile Asn Lys 165 170 175Val Tyr Met Gly Asn Gly Asn Tyr Gly Met Arg Thr Thr Ala Lys Ser 180 185190 Tyr Phe Gly Lys Asp Leu Lys Glu Leu Ser Ile Ala Gln Leu Ala Leu 195200 205 Leu Ala Gly Ile Pro Gln Ala Pro Thr Gln Tyr Asp Pro Tyr Lys Asn210 215 220 Pro Glu Ser Ala Gln Thr Arg Arg Asn Thr Val Leu Gln Gln MetTyr 225 230 235 240 Gln Asp Lys Asn Ile Ser Lys Lys Glu Tyr Asp Gln AlaVal Ala Thr 245 250 255 Pro Val Thr Asp Gly Leu Lys Glu Leu Lys Gln LysSer Thr Tyr Pro 260 265 270 Lys Tyr Met Asp Asn Tyr Leu Lys Gln Val IleSer Glu Val Lys Gln 275 280 285 Lys Thr Gly Lys Asp Ile Phe Thr Ala GlyLeu Lys Val Tyr Thr Asn 290 295 300 Ile Asn Thr Asp Ala Gln Lys Gln LeuTyr Asp Ile Tyr Asn Ser Asp 305 310 315 320 Thr Tyr Ile Ala Tyr Pro AsnAsn Glu Leu Gln Ile Ala Ser Thr Ile 325 330 335 Met Asp Ala Thr Asn GlyLys Val Ile Ala Gln Leu Gly Gly Arg His 340 345 350 Gln Asn Glu Asn IleSer Phe Gly Thr Asn Gln Ser Val Leu Thr Asp 355 360 365 Arg Asp Trp GlySer Thr Met Lys Pro Ile Ser Ala Tyr Ala Pro Ala 370 375 380 Ile Asp SerGly Val Tyr Asn Ser Thr Gly Gln Ser Leu Asn Asp Ser 385 390 395 400 ValTyr Tyr Trp Pro Gly Thr Ser Thr Gln Leu Tyr Asp Trp Asp Arg 405 410 415Gln Tyr Met Gly Trp Met Ser Met Gln Thr Ala Ile Gln Gln Ser Arg 420 425430 Asn Val Pro Ala Val Arg Ala Leu Glu Ala Ala Gly Leu Asp Glu Ala 435440 445 Lys Ser Phe Leu Glu Lys Leu Gly Ile Tyr Tyr Pro Glu Met 450 455460 93 336 DNA Streptococcus agalactiae 93 atggctaatg tatatgatttagcaaatgaa ttagaacgtg ctgttcgtgc tttaccagaa 60 taccaagcag ttttaactgcaaaagcagct attgaaaatg atgcggatgc acaagtgctt 120 tggcaagact ttttggctacccaatcaaaa gttcaagaaa tgatgcaatc tggccaaatg 180 ccaagtcaag aagaacaagatgaaatgtct aaacttgggg aaaaaattga atccaatgac 240 cttttaaaag tttattttgaccaacaacaa cggttgtctg tctatatgtc tgatatcgaa 300 aaaattgtct ttgcacccatgcaggacttg atgtaa 336 94 111 PRT Streptococcus agalactiae 94 Met Ala AsnVal Tyr Asp Leu Ala Asn Glu Leu Glu Arg Ala Val Arg 1 5 10 15 Ala LeuPro Glu Tyr Gln Ala Val Leu Thr Ala Lys Ala Ala Ile Glu 20 25 30 Asn AspAla Asp Ala Gln Val Leu Trp Gln Asp Phe Leu Ala Thr Gln 35 40 45 Ser LysVal Gln Glu Met Met Gln Ser Gly Gln Met Pro Ser Gln Glu 50 55 60 Glu GlnAsp Glu Met Ser Lys Leu Gly Glu Lys Ile Glu Ser Asn Asp 65 70 75 80 LeuLeu Lys Val Tyr Phe Asp Gln Gln Gln Arg Leu Ser Val Tyr Met 85 90 95 SerAsp Ile Glu Lys Ile Val Phe Ala Pro Met Gln Asp Leu Met 100 105 110 95230 DNA Streptococcus agalactiae 95 atggcagaaa tcacagctaa acttgtaaaagaattgcgtg aaaaatcagg tgcaggcgtt 60 atggacgcta aaaaagcatt agtagaaactgatggtgacc ttgataaagc gattgaatta 120 cttcgcgaaa aaggtatggc taaagcagctaaaaaagcag accgtgttgc tgctgaaggt 180 ttaacaggtg tttatgttga tggtaacgttgcagcagtta ttgaagttaa 230 96 76 PRT Streptococcus agalactiae 96 Met AlaGlu Ile Thr Ala Lys Leu Val Lys Glu Leu Arg Glu Lys Ser 1 5 10 15 GlyAla Gly Val Met Asp Ala Lys Lys Ala Leu Val Glu Thr Asp Gly 20 25 30 AspLeu Asp Lys Ala Ile Glu Leu Leu Arg Glu Lys Gly Met Ala Lys 35 40 45 AlaAla Lys Lys Ala Asp Arg Val Ala Ala Glu Gly Leu Thr Gly Val 50 55 60 TyrVal Asp Gly Asn Val Ala Ala Val Ile Glu Val 65 70 75 97 134 DNAStreptococcus agalactiae 97 atgataaaaa acctgttatt aacaggtttt ttatcatttaatgacggaaa actggacaca 60 aattattttt cttgtataat taaatatatt atttcttatcaggaggttat gatgacatta 120 gagaaacgat ttaa 134 98 44 PRT Streptococcusagalactiae 98 Met Ile Lys Asn Leu Leu Leu Thr Gly Phe Leu Ser Phe AsnAsp Gly 1 5 10 15 Lys Leu Asp Thr Asn Tyr Phe Ser Cys Ile Ile Lys TyrIle Ile Ser 20 25 30 Tyr Gln Glu Val Met Met Thr Leu Glu Lys Arg Phe 3540 99 94 DNA Streptococcus agalactiae 99 atgaaaaata ataaaaataatggttttctg aaaaattcct ttatttacat attattgatt 60 attgcggtta ttacaacctttcaatactat ttaa 94 100 31 PRT Streptococcus agalactiae 100 Met Lys AsnAsn Lys Asn Asn Gly Phe Leu Lys Asn Ser Phe Ile Tyr 1 5 10 15 Ile LeuLeu Ile Ile Ala Val Ile Thr Thr Phe Gln Tyr Tyr Leu 20 25 30 101 158 DNAStreptococcus agalactiae 101 atgttagata ttatcttatc cggaatttcg caaggattactttggtcaat tatggcaatt 60 ggcgtgttta tcacttttcg tatcttagac atagccgatctctctgcaga aggggctttc 120 cctatggggg ctgcagtttg cgccttatgt atcgttaa 158102 52 PRT Streptococcus agalactiae 102 Met Leu Asp Ile Ile Leu Ser GlyIle Ser Gln Gly Leu Leu Trp Ser 1 5 10 15 Ile Met Ala Ile Gly Val PheIle Thr Phe Arg Ile Leu Asp Ile Ala 20 25 30 Asp Leu Ser Ala Glu Gly AlaPhe Pro Met Gly Ala Ala Val Cys Ala 35 40 45 Leu Cys Ile Val 50 103 161DNA Streptococcus agalactiae 103 atggaaatgc ctaaaagaaa tgaattactcaataaagaaa ttaaaatgag tattgataaa 60 cttagatata aagaaccaga gagtgaacatgacaagcgac ctacttttta tttggtagta 120 cttatacttg ttactgtagc agttatattgtcgttattta a 161 104 53 PRT Streptococcus agalactiae 104 Met Glu Met ProLys Arg Asn Glu Leu Leu Asn Lys Glu Ile Lys Met 1 5 10 15 Ser Ile AspLys Leu Arg Tyr Lys Glu Pro Glu Ser Glu His Asp Lys 20 25 30 Arg Pro ThrPhe Tyr Leu Val Val Leu Ile Leu Val Thr Val Ala Val 35 40 45 Ile Leu SerLeu Phe 50 105 179 DNA Streptococcus agalactiae 105 gtggtaagtaaattgagttt aacaacgatt tttgcattgc tattttcatc aatgctaatt 60 tacgcaacacctcttatctt tacaagtatt gggggaacct tctctgaacg tggtggtatc 120 gtcaacgttggtttagaagg aattatggta attggagctt tctcaggcgt tgtatttaa 179 106 59 PRTStreptococcus agalactiae 106 Met Val Ser Lys Leu Ser Leu Thr Thr Ile PheAla Leu Leu Phe Ser 1 5 10 15 Ser Met Leu Ile Tyr Ala Thr Pro Leu IlePhe Thr Ser Ile Gly Gly 20 25 30 Thr Phe Ser Glu Arg Gly Gly Ile Val AsnVal Gly Leu Glu Gly Ile 35 40 45 Met Val Ile Gly Ala Phe Ser Gly Val ValPhe 50 55 107 558 DNA Streptococcus agalactiae 107 atgagaatta ttgcaataactgaaaaggtt ataaaagaac tgtttcgtga taaaagaaca 60 cttgctatga tgtttttagcacctatttta attatgtttt tgatgaatgt tatgttttct 120 gcgaatagta atacaaaagttaagattgga actattaacg ttaacacgaa ggtcgtttca 180 aatttagata atattaagcatattcaagtg agatcattta aatttaactc atctgctaaa 240 aaagcactca aatcaaataaaattgatgct cttatttcgg aggacaataa atcttatact 300 gtcttctatg cgaatacagattcttcaaag acgactttaa caagacaagc ttttaaaacc 360 gctgttaata caatgaacagtaaggaactg atttcgcaag ttaaaatttt agctaataag 420 aatccgaaac tagcacaatccttacaaact cgctccaaat atatcaaaga aaaatataat 480 tacggaaata aaaatacaggcttttttgca aaaatgatac caatactaat gggatttatg 540 gtcttcttct tggttttt 558108 186 PRT Streptococcus agalactiae 108 Met Arg Ile Ile Ala Ile Thr GluLys Val Ile Lys Glu Leu Phe Arg 1 5 10 15 Asp Lys Arg Thr Leu Ala MetMet Phe Leu Ala Pro Ile Leu Ile Met 20 25 30 Phe Leu Met Asn Val Met PheSer Ala Asn Ser Asn Thr Lys Val Lys 35 40 45 Ile Gly Thr Ile Asn Val AsnThr Lys Val Val Ser Asn Leu Asp Asn 50 55 60 Ile Lys His Ile Gln Val ArgSer Phe Lys Phe Asn Ser Ser Ala Lys 65 70 75 80 Lys Ala Leu Lys Ser AsnLys Ile Asp Ala Leu Ile Ser Glu Asp Asn 85 90 95 Lys Ser Tyr Thr Val PheTyr Ala Asn Thr Asp Ser Ser Lys Thr Thr 100 105 110 Leu Thr Arg Gln AlaPhe Lys Thr Ala Val Asn Thr Met Asn Ser Lys 115 120 125 Glu Leu Ile SerGln Val Lys Ile Leu Ala Asn Lys Asn Pro Lys Leu 130 135 140 Ala Gln SerLeu Gln Thr Arg Ser Lys Tyr Ile Lys Glu Lys Tyr Asn 145 150 155 160 TyrGly Asn Lys Asn Thr Gly Phe Phe Ala Lys Met Ile Pro Ile Leu 165 170 175Met Gly Phe Met Val Phe Phe Leu Val Phe 180 185 109 100 DNAStreptococcus agalactiae 109 gtgattatcg ttatgagtaa acatcaagaa attttggagtacctagaaaa tttagctgtt 60 ggtaagaggg ttagtgtacg cagtatttca aatcatttaa 100110 33 PRT Streptococcus agalactiae 110 Met Ile Ile Val Met Ser Lys HisGln Glu Ile Leu Glu Tyr Leu Glu 1 5 10 15 Asn Leu Ala Val Gly Lys ArgVal Ser Val Arg Ser Ile Ser Asn His 20 25 30 Leu 111 326 DNAStreptococcus agalactiae 111 atgtatagag aaattaccgc tgtcgaacac gatcgctttgtgagcgaatc caaccaaaca 60 aacctacttc aatctcttaa ttggcccaaa gtaaaagacaactggggtag tcaattactt 120 ggcttttttg acggtgaaac ccaaattgcc agcgctagtattctcatcaa atcacttcct 180 cttggcttct ccatgctgta tattccgcgt ggaccaatcatggattactc caatctagat 240 attgtaacta aggtccttaa ggaccttaaa gcttttggcaaaaaacaaag agctctcttt 300 atcaagtgtg atcctctcat ctattt 326 112 108 PRTStreptococcus agalactiae 112 Met Tyr Arg Glu Ile Thr Ala Val Glu His AspArg Phe Val Ser Glu 1 5 10 15 Ser Asn Gln Thr Asn Leu Leu Gln Ser LeuAsn Trp Pro Lys Val Lys 20 25 30 Asp Asn Trp Gly Ser Gln Leu Leu Gly PhePhe Asp Gly Glu Thr Gln 35 40 45 Ile Ala Ser Ala Ser Ile Leu Ile Lys SerLeu Pro Leu Gly Phe Ser 50 55 60 Met Leu Tyr Ile Pro Arg Gly Pro Ile MetAsp Tyr Ser Asn Leu Asp 65 70 75 80 Ile Val Thr Lys Val Leu Lys Asp LeuLys Ala Phe Gly Lys Lys Gln 85 90 95 Arg Ala Leu Phe Ile Lys Cys Asp ProLeu Ile Tyr 100 105 113 215 DNA Streptococcus agalactiae 113 atggacaagaaaaaaatctt agtaacgggt attgtgccta aagaaggtct aagaaagctt 60 atggaccgatttgatgttac ttattcagaa gatcgcccat tttcacgtga ctatgtgtta 120 gagcatttatctgaatatga cggatggtta ctcatgggac aaaaaggtga taaagagatg 180 attgatgcaggtgaaaactt acaaattatt tcttt 215 114 71 PRT Streptococcus agalactiae 114Met Asp Lys Lys Lys Ile Leu Val Thr Gly Ile Val Pro Lys Glu Gly 1 5 1015 Leu Arg Lys Leu Met Asp Arg Phe Asp Val Thr Tyr Ser Glu Asp Arg 20 2530 Pro Phe Ser Arg Asp Tyr Val Leu Glu His Leu Ser Glu Tyr Asp Gly 35 4045 Trp Leu Leu Met Gly Gln Lys Gly Asp Lys Glu Met Ile Asp Ala Gly 50 5560 Glu Asn Leu Gln Ile Ile Ser 65 70 115 459 DNA Streptococcusagalactiae 115 atttcgaaag atgactacca aaatattagt tttggacagg atccagaagttgttgattat 60 gctggtctgt ttgaaaaacg ccgtccagtt ttagaaaaag cagttaaaaatttcttgcaa 120 gaagagagag ctacgagaat gctatctgat ttcttgcaag aagaaaaatgggtaactgat 180 tttgctgaat ttatggcgat caaagaacat tttggtaata aggcgcttcaagaatgggat 240 gacaaggcta ttatacgccg cgaagaagaa gccttagcag gatatcgtcaaaagcttagt 300 gaagtgataa aatatcatga agtaacgcaa tatttctttt acaaacaatggtttgagtta 360 aaagaatatg ctaatgataa agggattcaa attatcggtg atatgccaatctacgtttct 420 gccgatagtg tagaagtttg gacaatgcct gaactgttt 459 116 153PRT Streptococcus agalactiae 116 Ile Ser Lys Asp Asp Tyr Gln Asn Ile SerPhe Gly Gln Asp Pro Glu 1 5 10 15 Val Val Asp Tyr Ala Gly Leu Phe GluLys Arg Arg Pro Val Leu Glu 20 25 30 Lys Ala Val Lys Asn Phe Leu Gln GluGlu Arg Ala Thr Arg Met Leu 35 40 45 Ser Asp Phe Leu Gln Glu Glu Lys TrpVal Thr Asp Phe Ala Glu Phe 50 55 60 Met Ala Ile Lys Glu His Phe Gly AsnLys Ala Leu Gln Glu Trp Asp 65 70 75 80 Asp Lys Ala Ile Ile Arg Arg GluGlu Glu Ala Leu Ala Gly Tyr Arg 85 90 95 Gln Lys Leu Ser Glu Val Ile LysTyr His Glu Val Thr Gln Tyr Phe 100 105 110 Phe Tyr Lys Gln Trp Phe GluLeu Lys Glu Tyr Ala Asn Asp Lys Gly 115 120 125 Ile Gln Ile Ile Gly AspMet Pro Ile Tyr Val Ser Ala Asp Ser Val 130 135 140 Glu Val Trp Thr MetPro Glu Leu Phe 145 150 117 1143 DNA Streptococcus agalactiae 117atggcaaaac agaaaaataa ctggcgccgt gttggagttg gtgtccttac acttgcttca 60gttgcgactc ttgctgcatg tggaagtaaa tcagcttccc aggattctaa tggagcgatt 120aattgggcta ttccaacaga aatcaataca ctagatttat ctaaagttac agacacttac 180tcaaatctag ctattggtaa ctctagtagt aatttccttc gcttagataa agatggaaag 240acaagaccag acttggctac taaagttgat gtttcaaaag atggcttaac ttatacagct 300acattacgta aaggcttgaa gtggtcagat ggcagtaaac ttactgcaaa ggattttgtt 360tattcatggc aacgtttagt tgatcctaaa acagcttcac aatatgctta ccttgctgtt 420gaagggcatg tgcttaatgc cgataaaatc aacgaaggac aagagaaaga cttgaataag 480ctaggtgtta aggcagaagg cgatgacaaa gttgttatta ctttatctag tccgtctccg 540caattcatct actaccttgc attcactaac ttcatgccac aaaaacaaga agttgttgaa 600aaatatggaa aagattacgc aactacttca aaaaatacag tttactcagg accatatact 660gttgaaggtt ggaatggttc gaatggtact ttcacgctga agaaaaacaa aaattattgg 720gacgctaaaa atgtaaaaac aaaagaagtt cgcatccaga ctgttaaaaa accagatacc 780gccgttcaaa tgtataaacg tggtgagtta gatgcagcta atatctcaaa tacttctgct 840atttatcaag ctaataaaaa taataaagat gtcacagatg ttctagaagc gaccactgcc 900tatatggaat ataatactac tggttctgtg aaagggcttg ataatgttaa gattcgtcgc 960gccttaaact tagcaactaa ccgtaaagga gttgttcaag cagccgttga tacaggctca 1020aaaccggcaa ttgcttttgc acctactggt ttagccaaaa caccagatgg aactgatttg 1080gcaaaatatg ttgccccagg ttatgaatat aataaaactg aagcagcaaa actctttaga 1140cta 1143 118 381 PRT Streptococcus agalactiae 118 Met Ala Lys Gln LysAsn Asn Trp Arg Arg Val Gly Val Gly Val Leu 1 5 10 15 Thr Leu Ala SerVal Ala Thr Leu Ala Ala Cys Gly Ser Lys Ser Ala 20 25 30 Ser Gln Asp SerAsn Gly Ala Ile Asn Trp Ala Ile Pro Thr Glu Ile 35 40 45 Asn Thr Leu AspLeu Ser Lys Val Thr Asp Thr Tyr Ser Asn Leu Ala 50 55 60 Ile Gly Asn SerSer Ser Asn Phe Leu Arg Leu Asp Lys Asp Gly Lys 65 70 75 80 Thr Arg ProAsp Leu Ala Thr Lys Val Asp Val Ser Lys Asp Gly Leu 85 90 95 Thr Tyr ThrAla Thr Leu Arg Lys Gly Leu Lys Trp Ser Asp Gly Ser 100 105 110 Lys LeuThr Ala Lys Asp Phe Val Tyr Ser Trp Gln Arg Leu Val Asp 115 120 125 ProLys Thr Ala Ser Gln Tyr Ala Tyr Leu Ala Val Glu Gly His Val 130 135 140Leu Asn Ala Asp Lys Ile Asn Glu Gly Gln Glu Lys Asp Leu Asn Lys 145 150155 160 Leu Gly Val Lys Ala Glu Gly Asp Asp Lys Val Val Ile Thr Leu Ser165 170 175 Ser Pro Ser Pro Gln Phe Ile Tyr Tyr Leu Ala Phe Thr Asn PheMet 180 185 190 Pro Gln Lys Gln Glu Val Val Glu Lys Tyr Gly Lys Asp TyrAla Thr 195 200 205 Thr Ser Lys Asn Thr Val Tyr Ser Gly Pro Tyr Thr ValGlu Gly Trp 210 215 220 Asn Gly Ser Asn Gly Thr Phe Thr Leu Lys Lys AsnLys Asn Tyr Trp 225 230 235 240 Asp Ala Lys Asn Val Lys Thr Lys Glu ValArg Ile Gln Thr Val Lys 245 250 255 Lys Pro Asp Thr Ala Val Gln Met TyrLys Arg Gly Glu Leu Asp Ala 260 265 270 Ala Asn Ile Ser Asn Thr Ser AlaIle Tyr Gln Ala Asn Lys Asn Asn 275 280 285 Lys Asp Val Thr Asp Val LeuGlu Ala Thr Thr Ala Tyr Met Glu Tyr 290 295 300 Asn Thr Thr Gly Ser ValLys Gly Leu Asp Asn Val Lys Ile Arg Arg 305 310 315 320 Ala Leu Asn LeuAla Thr Asn Arg Lys Gly Val Val Gln Ala Ala Val 325 330 335 Asp Thr GlySer Lys Pro Ala Ile Ala Phe Ala Pro Thr Gly Leu Ala 340 345 350 Lys ThrPro Asp Gly Thr Asp Leu Ala Lys Tyr Val Ala Pro Gly Tyr 355 360 365 GluTyr Asn Lys Thr Glu Ala Ala Lys Leu Phe Arg Leu 370 375 380 119 234 DNAStreptococcus agalactiae 119 ttgagagttt atgaaaataa agaagagttg aaaaaagaaataagtaaaac atttgagaaa 60 tacattatgg aatttaataa tattccagag aatctaaaagataaaagaat tgatgaagtt 120 gatagaactc cagcagaaaa cctttcttat caggttggctggaccaactt ggttcttaaa 180 tgggaagaag atgaaagaaa gggacttcaa gtaaaaacaccatcggataa attt 234 120 78 PRT Streptococcus agalactiae 120 Met Arg ValTyr Glu Asn Lys Glu Glu Leu Lys Lys Glu Ile Ser Lys 1 5 10 15 Thr PheGlu Lys Tyr Ile Met Glu Phe Asn Asn Ile Pro Glu Asn Leu 20 25 30 Lys AspLys Arg Ile Asp Glu Val Asp Arg Thr Pro Ala Glu Asn Leu 35 40 45 Ser TyrGln Val Gly Trp Thr Asn Leu Val Leu Lys Trp Glu Glu Asp 50 55 60 Glu ArgLys Gly Leu Gln Val Lys Thr Pro Ser Asp Lys Phe 65 70 75 121 150 DNAStreptococcus agalactiae 121 atgtcaaagt ttgatagtca gaaaataatt actccgattatgaagtttgt caatatgcga 60 gggattattg cactcaaaga tggcatgcta gcaattttaccactaacagt tgttgggagt 120 ctctttttaa tattagggca gcttccattt 150 122 50PRT Streptococcus agalactiae 122 Met Ser Lys Phe Asp Ser Gln Lys Ile IleThr Pro Ile Met Lys Phe 1 5 10 15 Val Asn Met Arg Gly Ile Ile Ala LeuLys Asp Gly Met Leu Ala Ile 20 25 30 Leu Pro Leu Thr Val Val Gly Ser LeuPhe Leu Ile Leu Gly Gln Leu 35 40 45 Pro Phe 50 123 535 DNAStreptococcus agalactiae 123 gagaccactt catcagttaa accagcagga attgaccgtatcaatcatac ctcaacaccc 60 ccgaagaaaa ctacccccaa cattgcaacg acgcatagcttcaaagatcg ttgtgatact 120 ttagaaagaa ttcacaatga agacattgat gtttgttctggattcatttg tggtatggga 180 gagagcgatg aggggctcat cacattagct ttcagactaaaagaactgaa cccctattct 240 atccctgtca attttttact tgctgttgaa ggaacacctcttggaaaata taactatttg 300 actcccatta aatgcttaaa aattatggcc atgttgcgttttgtttttcc tttcaaggaa 360 ttaagattaa gtgctggacg ggaggtccat tttgagaattttgaatcatt agtcacctta 420 cttgttgact caactttttt gggaaattac ctaacagaggggggtcgcaa tcaacatacc 480 gatattgaat tcttggaaaa attacaacta aatcatactaaaaaggaatt aattt 535 124 178 PRT Streptococcus agalactiae 124 Glu ThrThr Ser Ser Val Lys Pro Ala Gly Ile Asp Arg Ile Asn His 1 5 10 15 ThrSer Thr Pro Pro Lys Lys Thr Thr Pro Asn Ile Ala Thr Thr His 20 25 30 SerPhe Lys Asp Arg Cys Asp Thr Leu Glu Arg Ile His Asn Glu Asp 35 40 45 IleAsp Val Cys Ser Gly Phe Ile Cys Gly Met Gly Glu Ser Asp Glu 50 55 60 GlyLeu Ile Thr Leu Ala Phe Arg Leu Lys Glu Leu Asn Pro Tyr Ser 65 70 75 80Ile Pro Val Asn Phe Leu Leu Ala Val Glu Gly Thr Pro Leu Gly Lys 85 90 95Tyr Asn Tyr Leu Thr Pro Ile Lys Cys Leu Lys Ile Met Ala Met Leu 100 105110 Arg Phe Val Phe Pro Phe Lys Glu Leu Arg Leu Ser Ala Gly Arg Glu 115120 125 Val His Phe Glu Asn Phe Glu Ser Leu Val Thr Leu Leu Val Asp Ser130 135 140 Thr Phe Leu Gly Asn Tyr Leu Thr Glu Gly Gly Arg Asn Gln HisThr 145 150 155 160 Asp Ile Glu Phe Leu Glu Lys Leu Gln Leu Asn His ThrLys Lys Glu 165 170 175 Leu Ile 125 563 DNA Streptococcus agalactiae 125atgccggttt ggactgcaca gtctattcca aaggcatttt tagaaaagca taatactaag 60gaaggcacct gggcaaaact aaccattcta agtggttctt tagtatttta ccagttatct 120cctgatggag aggaaatctc gcggcatatt tttgatgcta gtagtgatat tccttttgtt 180gatccacaag tctggcataa agtttcgccg aatagtccag acttaagttg ctatctaact 240ttttactgcc aaaaagaaga ttacttccat aaaaaatatg gtctcacgcg cacacattct 300gaggttatcg ccagtgcacc tctcttatct gagaagagta atatattaga ccttgggtgt 360ggtcaagggc gaaactcact ttatttatcg ctgctgggac atcaagtgac ttctgtcgat 420tcaaacggac agagccttgt agctttagaa aatatggcat tagaagaaga gcttccttac 480aatataaaaa ggtatgatat taatactact gctattgaag ggcactatga ttttatttta 540tcaactgtgg tatttatgtt ttt 563 126 187 PRT Streptococcus agalactiae 126Met Pro Val Trp Thr Ala Gln Ser Ile Pro Lys Ala Phe Leu Glu Lys 1 5 1015 His Asn Thr Lys Glu Gly Thr Trp Ala Lys Leu Thr Ile Leu Ser Gly 20 2530 Ser Leu Val Phe Tyr Gln Leu Ser Pro Asp Gly Glu Glu Ile Ser Arg 35 4045 His Ile Phe Asp Ala Ser Ser Asp Ile Pro Phe Val Asp Pro Gln Val 50 5560 Trp His Lys Val Ser Pro Asn Ser Pro Asp Leu Ser Cys Tyr Leu Thr 65 7075 80 Phe Tyr Cys Gln Lys Glu Asp Tyr Phe His Lys Lys Tyr Gly Leu Thr 8590 95 Arg Thr His Ser Glu Val Ile Ala Ser Ala Pro Leu Leu Ser Glu Lys100 105 110 Ser Asn Ile Leu Asp Leu Gly Cys Gly Gln Gly Arg Asn Ser LeuTyr 115 120 125 Leu Ser Leu Leu Gly His Gln Val Thr Ser Val Asp Ser AsnGly Gln 130 135 140 Ser Leu Val Ala Leu Glu Asn Met Ala Leu Glu Glu GluLeu Pro Tyr 145 150 155 160 Asn Ile Lys Arg Tyr Asp Ile Asn Thr Thr AlaIle Glu Gly His Tyr 165 170 175 Asp Phe Ile Leu Ser Thr Val Val Phe MetPhe 180 185 127 417 DNA Streptococcus agalactiae 127 atgacaaagcaaataattgc catttgggct gaagatgaag accatttgat tggagttaat 60 ggcggtttaccatggaggct tcctaaagag ttacatcact tcaaagaaac gaccatgggg 120 caggctttgcttatgggacg aaagaccttt gatggaatga accgtcgtgt tttacctggt 180 agagagacaatcatcttaac aaaagatgaa caattccaag cagatggagt gacagtccta 240 aatagtgttgaacaagttat aaaatggttt caggaacata ataagacctt atttattgta 300 ggtggtgcaagtatttataa agcatttctg ccttattgtg aagcaatcat aaaaactaaa 360 gttcatggaaaattcaaagg tgatacctat tttcctgatg ttaatctatc tgagttt 417 128 139 PRTStreptococcus agalactiae 128 Met Thr Lys Gln Ile Ile Ala Ile Trp Ala GluAsp Glu Asp His Leu 1 5 10 15 Ile Gly Val Asn Gly Gly Leu Pro Trp ArgLeu Pro Lys Glu Leu His 20 25 30 His Phe Lys Glu Thr Thr Met Gly Gln AlaLeu Leu Met Gly Arg Lys 35 40 45 Thr Phe Asp Gly Met Asn Arg Arg Val LeuPro Gly Arg Glu Thr Ile 50 55 60 Ile Leu Thr Lys Asp Glu Gln Phe Gln AlaAsp Gly Val Thr Val Leu 65 70 75 80 Asn Ser Val Glu Gln Val Ile Lys TrpPhe Gln Glu His Asn Lys Thr 85 90 95 Leu Phe Ile Val Gly Gly Ala Ser IleTyr Lys Ala Phe Leu Pro Tyr 100 105 110 Cys Glu Ala Ile Ile Lys Thr LysVal His Gly Lys Phe Lys Gly Asp 115 120 125 Thr Tyr Phe Pro Asp Val AsnLeu Ser Glu Phe 130 135 129 543 DNA Streptococcus agalactiae 129ttgtggccaa actgtgcccc gcttattaat agcactttgt tcaccattga agatatctta 60acatcaggtg ctcatagcaa ccctatttta atgggggtta tacttggcgg gacaattgta 120gtagtggcga cagcaccact ttcttctatg gcattgacag ctatgctagg attaaccgga 180atgcctatgg ctataggagc cttgtctgtc tttggttcgt catttatgaa tggtgtactt 240ttccataaat taaaacttgg aagtcgtaaa gataatatag cttttgctgt tgagcctcta 300actcaagctg acgtgacttc agctaaccct attccaatct atgtcactaa ttttgttggt 360ggtgcagctt gtggtatttt aattgccttg atgaaattag ttaatgatac tcctggaaca 420gcgacaccaa ttgcaggatt tgctgtcatg tttgcctata acccaatgat aaaagtacta 480ataaccgctc taggttgtat tatcctatct ttactagcag gctattttgg aggcattgtt 540ttt 543 130 181 PRT Streptococcus agalactiae 130 Met Trp Pro Asn Cys AlaPro Leu Ile Asn Ser Thr Leu Phe Thr Ile 1 5 10 15 Glu Asp Ile Leu ThrSer Gly Ala His Ser Asn Pro Ile Leu Met Gly 20 25 30 Val Ile Leu Gly GlyThr Ile Val Val Val Ala Thr Ala Pro Leu Ser 35 40 45 Ser Met Ala Leu ThrAla Met Leu Gly Leu Thr Gly Met Pro Met Ala 50 55 60 Ile Gly Ala Leu SerVal Phe Gly Ser Ser Phe Met Asn Gly Val Leu 65 70 75 80 Phe His Lys LeuLys Leu Gly Ser Arg Lys Asp Asn Ile Ala Phe Ala 85 90 95 Val Glu Pro LeuThr Gln Ala Asp Val Thr Ser Ala Asn Pro Ile Pro 100 105 110 Ile Tyr ValThr Asn Phe Val Gly Gly Ala Ala Cys Gly Ile Leu Ile 115 120 125 Ala LeuMet Lys Leu Val Asn Asp Thr Pro Gly Thr Ala Thr Pro Ile 130 135 140 AlaGly Phe Ala Val Met Phe Ala Tyr Asn Pro Met Ile Lys Val Leu 145 150 155160 Ile Thr Ala Leu Gly Cys Ile Ile Leu Ser Leu Leu Ala Gly Tyr Phe 165170 175 Gly Gly Ile Val Phe 180 131 172 DNA Streptococcus agalactiae 131atgtttttaa gtataatggc aggtgtcata gcatttgtcc tgacagttat tgccattcca 60cgcttcatta agttttacca attgaagaaa attggcgggc aacaaatgca tgaagatgtc 120aaacaacatc tagccaaagc aggtacgccg acaatgggag gaacggtatt tt 172 132 57 PRTStreptococcus agalactiae 132 Met Phe Leu Ser Ile Met Ala Gly Val Ile AlaPhe Val Leu Thr Val 1 5 10 15 Ile Ala Ile Pro Arg Phe Ile Lys Phe TyrGln Leu Lys Lys Ile Gly 20 25 30 Gly Gln Gln Met His Glu Asp Val Lys GlnHis Leu Ala Lys Ala Gly 35 40 45 Thr Pro Thr Met Gly Gly Thr Val Phe 5055 133 113 DNA Streptococcus agalactiae 133 atgaaaccat atttatcttttattggtaga acgttattat acttcggtat tttattgtta 60 ctaatttact tttttgcataccttggtcgc ggacaaggca gttttattta taa 113 134 37 PRT Streptococcusagalactiae 134 Met Lys Pro Tyr Leu Ser Phe Ile Gly Arg Thr Leu Leu TyrPhe Gly 1 5 10 15 Ile Leu Leu Leu Leu Ile Tyr Phe Phe Ala Tyr Leu GlyArg Gly Gln 20 25 30 Gly Ser Phe Ile Tyr 35 135 651 DNA Streptococcusagalactiae 135 atgtcatatt ttagaaatta ctggtatcgt tttggagcaa ttttatttattattttagca 60 gtaatattgc ttgtttttag acctgactgg tcaatgcttc actatctattgtatttttac 120 tttatggcac ttctagcgca tcaatttgaa gaatatcagt ttcccggtggggcatcacct 180 atcattaact atgttgttta tgatgaagaa gagctgatgg attgttttccaggcaatact 240 cagtctatta tgttggttaa tactattgct tggttgcttt acattgctagtattgctttt 300 cctcaagctt attggcttgg attaggagtc atgttcttta gtctaacgcagctcttgggt 360 catggttttc agatgaatat taaacttaaa acttggtata atcctggtctagcaacgaca 420 gtatttctcc tagtaccaat agcttgcgca tacatctatc aagctagtgcagaaggaatg 480 ctcacttggg gagattggct aggtggtttt atcatgttga ttgtctgtgtactaactagc 540 attattgcac ctgtacagct attgaaggat aaggagacca attatattattagtccttgg 600 caaatggacc gttttcataa ggtcgttaat tttgtaagga taaaaaaata a651 136 216 PRT Streptococcus agalactiae 136 Met Ser Tyr Phe Arg Asn TyrTrp Tyr Arg Phe Gly Ala Ile Leu Phe 1 5 10 15 Ile Ile Leu Ala Val IleLeu Leu Val Phe Arg Pro Asp Trp Ser Met 20 25 30 Leu His Tyr Leu Leu TyrPhe Tyr Phe Met Ala Leu Leu Ala His Gln 35 40 45 Phe Glu Glu Tyr Gln PhePro Gly Gly Ala Ser Pro Ile Ile Asn Tyr 50 55 60 Val Val Tyr Asp Glu GluGlu Leu Met Asp Cys Phe Pro Gly Asn Thr 65 70 75 80 Gln Ser Ile Met LeuVal Asn Thr Ile Ala Trp Leu Leu Tyr Ile Ala 85 90 95 Ser Ile Ala Phe ProGln Ala Tyr Trp Leu Gly Leu Gly Val Met Phe 100 105 110 Phe Ser Leu ThrGln Leu Leu Gly His Gly Phe Gln Met Asn Ile Lys 115 120 125 Leu Lys ThrTrp Tyr Asn Pro Gly Leu Ala Thr Thr Val Phe Leu Leu 130 135 140 Val ProIle Ala Cys Ala Tyr Ile Tyr Gln Ala Ser Ala Glu Gly Met 145 150 155 160Leu Thr Trp Gly Asp Trp Leu Gly Gly Phe Ile Met Leu Ile Val Cys 165 170175 Val Leu Thr Ser Ile Ile Ala Pro Val Gln Leu Leu Lys Asp Lys Glu 180185 190 Thr Asn Tyr Ile Ile Ser Pro Trp Gln Met Asp Arg Phe His Lys Val195 200 205 Val Asn Phe Val Arg Ile Lys Lys 210 215 137 75 DNAStreptococcus agalactiae 137 atgccactta cagcacttga aattaaagat aaaacattttcatcaaaatt tcgcggttat 60 agcgaagaag aagtt 75 138 25 PRT Streptococcusagalactiae 138 Met Pro Leu Thr Ala Leu Glu Ile Lys Asp Lys Thr Phe SerSer Lys 1 5 10 15 Phe Arg Gly Tyr Ser Glu Glu Glu Val 20 25 139 377 DNAStreptococcus agalactiae 139 atgtcacttt ttcaagaaaa aattgcttac aattgcgctaaaaaggaagc gctttataaa 60 gagagtttag gacgctacgc cttgagatca atgctagcaggggcttattt gacaatgagt 120 actgctgccg gtatcgtcgc agctgatact attggtaaaatttctcctgc tctatcaggt 180 tttgtatttg ctttcatctt tagttttgga cttatttatgttttaatatt taatggtgaa 240 ttggcgacat ctaatatgct ttatctcact gcaggagcctataataaaaa tatctcttgg 300 aaaaaagcca taacaatttt aatttattgt acttttttcaacctcgttgg tgcttgtata 360 ttagcttggt tgtttaa 377 140 125 PRTStreptococcus agalactiae 140 Met Ser Leu Phe Gln Glu Lys Ile Ala Tyr AsnCys Ala Lys Lys Glu 1 5 10 15 Ala Leu Tyr Lys Glu Ser Leu Gly Arg TyrAla Leu Arg Ser Met Leu 20 25 30 Ala Gly Ala Tyr Leu Thr Met Ser Thr AlaAla Gly Ile Val Ala Ala 35 40 45 Asp Thr Ile Gly Lys Ile Ser Pro Ala LeuSer Gly Phe Val Phe Ala 50 55 60 Phe Ile Phe Ser Phe Gly Leu Ile Tyr ValLeu Ile Phe Asn Gly Glu 65 70 75 80 Leu Ala Thr Ser Asn Met Leu Tyr LeuThr Ala Gly Ala Tyr Asn Lys 85 90 95 Asn Ile Ser Trp Lys Lys Ala Ile ThrIle Leu Ile Tyr Cys Thr Phe 100 105 110 Phe Asn Leu Val Gly Ala Cys IleLeu Ala Trp Leu Phe 115 120 125 141 419 DNA Streptococcus agalactiae 141aagttacaag cgactgaagt taagagcgtt ccggtagcac aaccagcttc aacaacaaat 60gcagtagctg cacatcctga aaatgcaggg ctccaacctc atgttgcagc ttataaagaa 120aaagtagcgt caacttatgg agttaatgaa ttcagtacat accgtgcggg agatccaggt 180gatcatggta aaggtttagc agttgacttt attgtaggta aaaaccaagc acttggtaat 240gaagttgcac agtactctac acaaaatatg gcagcaaata acatttcata tgttatctgg 300caacaaaagt tttattcaaa tacaaatagt atttatggac ctgctaatac ttggaatgca 360atgccagatc gtggtggcgt tactgccaac cactatgacc acgttcacgt atcatttaa 419 142139 PRT Streptococcus agalactiae 142 Lys Leu Gln Ala Thr Glu Val Lys SerVal Pro Val Ala Gln Pro Ala 1 5 10 15 Ser Thr Thr Asn Ala Val Ala AlaHis Pro Glu Asn Ala Gly Leu Gln 20 25 30 Pro His Val Ala Ala Tyr Lys GluLys Val Ala Ser Thr Tyr Gly Val 35 40 45 Asn Glu Phe Ser Thr Tyr Arg AlaGly Asp Pro Gly Asp His Gly Lys 50 55 60 Gly Leu Ala Val Asp Phe Ile ValGly Lys Asn Gln Ala Leu Gly Asn 65 70 75 80 Glu Val Ala Gln Tyr Ser ThrGln Asn Met Ala Ala Asn Asn Ile Ser 85 90 95 Tyr Val Ile Trp Gln Gln LysPhe Tyr Ser Asn Thr Asn Ser Ile Tyr 100 105 110 Gly Pro Ala Asn Thr TrpAsn Ala Met Pro Asp Arg Gly Gly Val Thr 115 120 125 Ala Asn His Tyr AspHis Val His Val Ser Phe 130 135 143 693 DNA Streptococcus agalactiae 143atgattccag tagttattga acaaacaagt cgtggtgaac gttcttatga tatttactca 60cgtcttttaa aagatcgtat tattatgttg acaggccaag ttgaggataa tatggccaat 120agtatcattg cacagttatt gtttctcgat gcacaagata atacaaagga tatttacctt 180tatgtcaata caccaggtgg ttcagtatcg gctggacttg ctattgtgga caccatgaac 240ttcattaaat cggacgtaca gacgattgtt atggggatgg ctgcttcgat gggaaccatt 300attgcttcaa gtggtgctaa aggaaaacgt tttatgttac cgaatgcaga atatatgatc 360caccaaccaa tgggcggaac aggcggaggt acacagcaat ctgatatggc tatcgctgct 420gagcatcttt taaaaacgcg tcatacttta gaaaaaatct tagctgataa ttctggtcaa 480tctattgaaa aagtccatga tgatgcagag cgtgatcgtt ggatgagtgc tcaagaacac 540ttgattatgg ctttattgat gctattatgg aaaataataa tttacaataa tagatttaaa 600agagttgagt ttaccaactc tttttttatt tgttggaatt atgttataat cttagtaatt 660acagatatga cgcagaaagg aaaaaattat tga 693 144 230 PRT Streptococcusagalactiae 144 Met Ile Pro Val Val Ile Glu Gln Thr Ser Arg Gly Glu ArgSer Tyr 1 5 10 15 Asp Ile Tyr Ser Arg Leu Leu Lys Asp Arg Ile Ile MetLeu Thr Gly 20 25 30 Gln Val Glu Asp Asn Met Ala Asn Ser Ile Ile Ala GlnLeu Leu Phe 35 40 45 Leu Asp Ala Gln Asp Asn Thr Lys Asp Ile Tyr Leu TyrVal Asn Thr 50 55 60 Pro Gly Gly Ser Val Ser Ala Gly Leu Ala Ile Val AspThr Met Asn 65 70 75 80 Phe Ile Lys Ser Asp Val Gln Thr Ile Val Met GlyMet Ala Ala Ser 85 90 95 Met Gly Thr Ile Ile Ala Ser Ser Gly Ala Lys GlyLys Arg Phe Met 100 105 110 Leu Pro Asn Ala Glu Tyr Met Ile His Gln ProMet Gly Gly Thr Gly 115 120 125 Gly Gly Thr Gln Gln Ser Asp Met Ala IleAla Ala Glu His Leu Leu 130 135 140 Lys Thr Arg His Thr Leu Glu Lys IleLeu Ala Asp Asn Ser Gly Gln 145 150 155 160 Ser Ile Glu Lys Val His AspAsp Ala Glu Arg Asp Arg Trp Met Ser 165 170 175 Ala Gln Glu His Leu IleMet Ala Leu Leu Met Leu Leu Trp Lys Ile 180 185 190 Ile Ile Tyr Asn AsnArg Phe Lys Arg Val Glu Phe Thr Asn Ser Phe 195 200 205 Phe Ile Cys TrpAsn Tyr Val Ile Ile Leu Val Ile Thr Asp Met Thr 210 215 220 Gln Lys GlyLys Asn Tyr 225 230 145 459 DNA Streptococcus agalactiae 145 atgaaaccaaaaattattgg tgtacttggt ctaggaatat ttggacaaac actcgcacaa 60 gaactaagtaactttgaaca agatgttatt gctattgaca gcaatcctga aaatgtacaa 120 gctgtcgccgaagttgttac aaaagcagct atcggagaca ttactgattt agctttccta 180 aaacacatcgggatcagtga ctgtgatact gttattattg ctacaggaaa cagtttagag 240 agctcagtattggccgtaat gcactgtaaa aagttaggcg tcccacaagt tattgctaaa 300 gctcgaaaccttgtatacga agaagtactt tatgaaattg gtgctgattt ggttatctct 360 ccggagcgagaatctgggca aaatgttgct gcaaacctca tgagaaataa aattacagat 420 gtcttccagattgaatctga tatttctgtc attgaattt 459 146 153 PRT Streptococcus agalactiae146 Met Lys Pro Lys Ile Ile Gly Val Leu Gly Leu Gly Ile Phe Gly Gln 1 510 15 Thr Leu Ala Gln Glu Leu Ser Asn Phe Glu Gln Asp Val Ile Ala Ile 2025 30 Asp Ser Asn Pro Glu Asn Val Gln Ala Val Ala Glu Val Val Thr Lys 3540 45 Ala Ala Ile Gly Asp Ile Thr Asp Leu Ala Phe Leu Lys His Ile Gly 5055 60 Ile Ser Asp Cys Asp Thr Val Ile Ile Ala Thr Gly Asn Ser Leu Glu 6570 75 80 Ser Ser Val Leu Ala Val Met His Cys Lys Lys Leu Gly Val Pro Gln85 90 95 Val Ile Ala Lys Ala Arg Asn Leu Val Tyr Glu Glu Val Leu Tyr Glu100 105 110 Ile Gly Ala Asp Leu Val Ile Ser Pro Glu Arg Glu Ser Gly GlnAsn 115 120 125 Val Ala Ala Asn Leu Met Arg Asn Lys Ile Thr Asp Val PheGln Ile 130 135 140 Glu Ser Asp Ile Ser Val Ile Glu Phe 145 150 147 330DNA Streptococcus agalactiae 147 gtgcgttata gtaaagagat tattcagttagctataccag ctatgattga aaatatctta 60 caaatgctca tgggagtagt tgataattatctagtggctc agttaggtgt tgtagcagta 120 tcaggtgttt cagttgctaa taatataattactatttatc aagctatttt tatagcttta 180 ggggcgagta tagcaagtct attggccaagtcgttagcag gtagtgagaa ggatgatgca 240 atttcagtat gttctcaagc catttttctaacatcactga taggggcagt attaggaatt 300 atctcgattg tttttggaca aactttcttt330 148 110 PRT Streptococcus agalactiae 148 Met Arg Tyr Ser Lys Glu IleIle Gln Leu Ala Ile Pro Ala Met Ile 1 5 10 15 Glu Asn Ile Leu Gln MetLeu Met Gly Val Val Asp Asn Tyr Leu Val 20 25 30 Ala Gln Leu Gly Val ValAla Val Ser Gly Val Ser Val Ala Asn Asn 35 40 45 Ile Ile Thr Ile Tyr GlnAla Ile Phe Ile Ala Leu Gly Ala Ser Ile 50 55 60 Ala Ser Leu Leu Ala LysSer Leu Ala Gly Ser Glu Lys Asp Asp Ala 65 70 75 80 Ile Ser Val Cys SerGln Ala Ile Phe Leu Thr Ser Leu Ile Gly Ala 85 90 95 Val Leu Gly Ile IleSer Ile Val Phe Gly Gln Thr Phe Phe 100 105 110 149 240 DNAStreptococcus agalactiae 149 ttgattaaca agtattcgtg ctttttgaag aggattctccataataatac tcctttaata 60 gttatcgtga gaagtatttt aaagaaaaac cgccaaggtagagcgacatt tctgccttta 120 actacaataa aaccaagaga attagcacaa cattatctctcaaaattaca aagttctcaa 180 gggtttttag gaatagctag tgaattggta acctatgatcaacgcttgtc aaacattttt 240 150 80 PRT Streptococcus agalactiae 150 MetIle Asn Lys Tyr Ser Cys Phe Leu Lys Arg Ile Leu His Asn Asn 1 5 10 15Thr Pro Leu Ile Val Ile Val Arg Ser Ile Leu Lys Lys Asn Arg Gln 20 25 30Gly Arg Ala Thr Phe Leu Pro Leu Thr Thr Ile Lys Pro Arg Glu Leu 35 40 45Ala Gln His Tyr Leu Ser Lys Leu Gln Ser Ser Gln Gly Phe Leu Gly 50 55 60Ile Ala Ser Glu Leu Val Thr Tyr Asp Gln Arg Leu Ser Asn Ile Phe 65 70 7580 151 649 DNA Streptococcus agalactiae 151 ttgttgactc acaaaaatatattattaacc attatatttg gattatttat gattatatta 60 tcagcatgtg gtatgtctaataaggaaatg gctggtattg ataattggga acattatcaa 120 aaggaaaaga aaattactattggatttgat aatacttttg ttcctatggg atttgaaagt 180 cgttctggtg actataccggctttgatatt gatttagcta atgctgtttt taaagaatac 240 ggtatttcag tgaaatggcagcctattaac tgggatatga aagaaactga acttaataat 300 ggtaatatag accttatttggaatggttat tcaaaaacgg cagaacgtgc taaaaaagtc 360 gcttttacaa acccatatatgaataatcat caagtaattg ttactaaaac ttcatcacat 420 attaatagta ttaaggatatgaaggggaaa aaactaggag cccagtcggg ttcatctggt 480 tttgatgctt ttaacgctaaacctgatatt ttaaaaaagt ttgtaaaagg aaaagaagca 540 gttcaatacg atactttcactcaggctttg attgatttaa aaaataaccg tattgatggt 600 cttttgattg atgaagtttatgctaactat tatttaaagc aagaaggaa 649 152 216 PRT Streptococcus agalactiae152 Met Leu Thr His Lys Asn Ile Leu Leu Thr Ile Ile Phe Gly Leu Phe 1 510 15 Met Ile Ile Leu Ser Ala Cys Gly Met Ser Asn Lys Glu Met Ala Gly 2025 30 Ile Asp Asn Trp Glu His Tyr Gln Lys Glu Lys Lys Ile Thr Ile Gly 3540 45 Phe Asp Asn Thr Phe Val Pro Met Gly Phe Glu Ser Arg Ser Gly Asp 5055 60 Tyr Thr Gly Phe Asp Ile Asp Leu Ala Asn Ala Val Phe Lys Glu Tyr 6570 75 80 Gly Ile Ser Val Lys Trp Gln Pro Ile Asn Trp Asp Met Lys Glu Thr85 90 95 Glu Leu Asn Asn Gly Asn Ile Asp Leu Ile Trp Asn Gly Tyr Ser Lys100 105 110 Thr Ala Glu Arg Ala Lys Lys Val Ala Phe Thr Asn Pro Tyr MetAsn 115 120 125 Asn His Gln Val Ile Val Thr Lys Thr Ser Ser His Ile AsnSer Ile 130 135 140 Lys Asp Met Lys Gly Lys Lys Leu Gly Ala Gln Ser GlySer Ser Gly 145 150 155 160 Phe Asp Ala Phe Asn Ala Lys Pro Asp Ile LeuLys Lys Phe Val Lys 165 170 175 Gly Lys Glu Ala Val Gln Tyr Asp Thr PheThr Gln Ala Leu Ile Asp 180 185 190 Leu Lys Asn Asn Arg Ile Asp Gly LeuLeu Ile Asp Glu Val Tyr Ala 195 200 205 Asn Tyr Tyr Leu Lys Gln Glu Gly210 215 153 123 DNA Streptococcus agalactiae 153 atgaaaattt ggaaaaaaataaccttaatg ttttctgcaa ttattttaac aacagtaatt 60 gcattgggag tctatgttgcctcagcttat aatttttcga ctaatgaatt gtctaagact 120 ttt 123 154 41 PRTStreptococcus agalactiae 154 Met Lys Ile Trp Lys Lys Ile Thr Leu Met PheSer Ala Ile Ile Leu 1 5 10 15 Thr Thr Val Ile Ala Leu Gly Val Tyr ValAla Ser Ala Tyr Asn Phe 20 25 30 Ser Thr Asn Glu Leu Ser Lys Thr Phe 3540 155 687 DNA Streptococcus agalactiae 155 atgaaaaaac aaagactattactgcttttt ggaggcttat taataatgat aatgatgaca 60 gcatgtaagg attcaaaaatcccagaaaac cgcacgaaaa aggaatacca ggcagaacag 120 aattttaagt catactttaaatatatatca gataaaaata actatttaga taatataaaa 180 gtttattact tttctataagtatttctaaa gatgtacaag ataaagtcag tgaaacaaca 240 acttgttcat atagactagaaaagcaaaag aatcaagagt tcattggtaa ttttgaacat 300 gaagttagtg aatctagtcaatattcaacc gaagttaaaa atcaaataca gtatccaatc 360 cagtataaag ataattcaattcgttttact gaaaaaacac cgtcagaacg ttatgatgag 420 tttgttttta gttcatttgattcttcatta ttaaaaaaat ataaaatata tgattactta 480 ctaaaacatc ccgaaactgaattaaaaggt gtttcctata agattcctat aaattctgaa 540 attgtagccc cttttataaatcaattaaat ataaaaaatc ctaaaaaatc atctatttcg 600 gttacaaaaa cggaaagtaaagaatattat tatacaatca gtattgatac tgattctgag 660 atatattcta tattcgaaggtattcat 687 156 229 PRT Streptococcus agalactiae 156 Met Lys Lys Gln ArgLeu Leu Leu Leu Phe Gly Gly Leu Leu Ile Met 1 5 10 15 Ile Met Met ThrAla Cys Lys Asp Ser Lys Ile Pro Glu Asn Arg Thr 20 25 30 Lys Lys Glu TyrGln Ala Glu Gln Asn Phe Lys Ser Tyr Phe Lys Tyr 35 40 45 Ile Ser Asp LysAsn Asn Tyr Leu Asp Asn Ile Lys Val Tyr Tyr Phe 50 55 60 Ser Ile Ser IleSer Lys Asp Val Gln Asp Lys Val Ser Glu Thr Thr 65 70 75 80 Thr Cys SerTyr Arg Leu Glu Lys Gln Lys Asn Gln Glu Phe Ile Gly 85 90 95 Asn Phe GluHis Glu Val Ser Glu Ser Ser Gln Tyr Ser Thr Glu Val 100 105 110 Lys AsnGln Ile Gln Tyr Pro Ile Gln Tyr Lys Asp Asn Ser Ile Arg 115 120 125 PheThr Glu Lys Thr Pro Ser Glu Arg Tyr Asp Glu Phe Val Phe Ser 130 135 140Ser Phe Asp Ser Ser Leu Leu Lys Lys Tyr Lys Ile Tyr Asp Tyr Leu 145 150155 160 Leu Lys His Pro Glu Thr Glu Leu Lys Gly Val Ser Tyr Lys Ile Pro165 170 175 Ile Asn Ser Glu Ile Val Ala Pro Phe Ile Asn Gln Leu Asn IleLys 180 185 190 Asn Pro Lys Lys Ser Ser Ile Ser Val Thr Lys Thr Glu SerLys Glu 195 200 205 Tyr Tyr Tyr Thr Ile Ser Ile Asp Thr Asp Ser Glu IleTyr Ser Ile 210 215 220 Phe Glu Gly Ile His 225 157 272 DNAStreptococcus agalactiae 157 atgacatttg acaccattga tcaattagcg gttaatacagtccgcacgct ttctattgat 60 gctatccaag cagcaaattc tgggcaccca ggtcttcctatgggagctgc gcctatggct 120 tatgtgcttt ggaataaatt cttaaatgta aacccaaaaacaagtcgcaa ttggacaaac 180 cgtgaccgtt ttgtactttc agctgggcat ggttcagctcttctttatag cctacttcat 240 ttagctggct atgatttatc aattgatgat tt 272 158 90PRT Streptococcus agalactiae 158 Met Thr Phe Asp Thr Ile Asp Gln Leu AlaVal Asn Thr Val Arg Thr 1 5 10 15 Leu Ser Ile Asp Ala Ile Gln Ala AlaAsn Ser Gly His Pro Gly Leu 20 25 30 Pro Met Gly Ala Ala Pro Met Ala TyrVal Leu Trp Asn Lys Phe Leu 35 40 45 Asn Val Asn Pro Lys Thr Ser Arg AsnTrp Thr Asn Arg Asp Arg Phe 50 55 60 Val Leu Ser Ala Gly His Gly Ser AlaLeu Leu Tyr Ser Leu Leu His 65 70 75 80 Leu Ala Gly Tyr Asp Leu Ser IleAsp Asp 85 90 159 197 DNA Streptococcus agalactiae 159 atgagaacactatttagaat gatatttgct attccaaagt ttatctttag attgatttgg 60 aatatcatttggggaatatt caagacagtt cttgttattg cgattatttt atttggcttg 120 tattactatgcgaatcacag tcaatcagaa tttgctaatc aacttagtga cattattcag 180 acaggaaaaacattttt 197 160 65 PRT Streptococcus agalactiae 160 Met Arg Thr Leu PheArg Met Ile Phe Ala Ile Pro Lys Phe Ile Phe 1 5 10 15 Arg Leu Ile TrpAsn Ile Ile Trp Gly Ile Phe Lys Thr Val Leu Val 20 25 30 Ile Ala Ile IleLeu Phe Gly Leu Tyr Tyr Tyr Ala Asn His Ser Gln 35 40 45 Ser Glu Phe AlaAsn Gln Leu Ser Asp Ile Ile Gln Thr Gly Lys Thr 50 55 60 Phe 65 161 153DNA Streptococcus agalactiae 161 atgtcaaaaa aaataatatt aggaattttatctcttttat ctgtcgttac tttggtggcg 60 tgtggttcat cagacaaaca gctacaagataaagttgaga aaaaagggaa gttagtttta 120 gcggtgagtc cagattatgc tccctttgagttt 153 162 51 PRT Streptococcus agalactiae 162 Met Ser Lys Lys Ile IleLeu Gly Ile Leu Ser Leu Leu Ser Val Val 1 5 10 15 Thr Leu Val Ala CysGly Ser Ser Asp Lys Gln Leu Gln Asp Lys Val 20 25 30 Glu Lys Lys Gly LysLeu Val Leu Ala Val Ser Pro Asp Tyr Ala Pro 35 40 45 Phe Glu Phe 50 163138 DNA Streptococcus agalactiae 163 atgaaaaatc aaagactatt actgctttttggaggcttat taataatgat aatgatgaca 60 gcatgtaagg attcaaaaat cccagaaaaccgcacgaaaa aggaatacca ggcagaacag 120 aattttaagt catacttt 138 164 46 PRTStreptococcus agalactiae 164 Met Lys Asn Gln Arg Leu Leu Leu Leu Phe GlyGly Leu Leu Ile Met 1 5 10 15 Ile Met Met Thr Ala Cys Lys Asp Ser LysIle Pro Glu Asn Arg Thr 20 25 30 Lys Lys Glu Tyr Gln Ala Glu Gln Asn PheLys Ser Tyr Phe 35 40 45 165 423 DNA Streptococcus agalactiae 165atgattggaa aattatatta tagctataga aagtcacgct tattaagaag tattttatgg 60cttattttaa ttgttggtgt atatatgtta ggacaacgtg ttttattatc cactgttcct 120ttatcacatc aagagataaa actagcagta gatcaacatt tactcaataa cttttcagca 180gtaagtggtg ggagttttaa taaattaaat gttttcacac tggggttgag tccatggatg 240tcaagtatga ttatttggag attcgtttcc ttattttcgt gggcaaaaaa tgcaacgaag 300cgaaaagcag aagtagctca atatacttta atgcttacta tctcagttat acaagcatat 360ggtgtttcag gaaatcaatt tataaaaagc tctttattag gttcttatag tgatattgtt 420ttt 423 166 141 PRT Streptococcus agalactiae 166 Met Ile Gly Lys Leu TyrTyr Ser Tyr Arg Lys Ser Arg Leu Leu Arg 1 5 10 15 Ser Ile Leu Trp LeuIle Leu Ile Val Gly Val Tyr Met Leu Gly Gln 20 25 30 Arg Val Leu Leu SerThr Val Pro Leu Ser His Gln Glu Ile Lys Leu 35 40 45 Ala Val Asp Gln HisLeu Leu Asn Asn Phe Ser Ala Val Ser Gly Gly 50 55 60 Ser Phe Asn Lys LeuAsn Val Phe Thr Leu Gly Leu Ser Pro Trp Met 65 70 75 80 Ser Ser Met IleIle Trp Arg Phe Val Ser Leu Phe Ser Trp Ala Lys 85 90 95 Asn Ala Thr LysArg Lys Ala Glu Val Ala Gln Tyr Thr Leu Met Leu 100 105 110 Thr Ile SerVal Ile Gln Ala Tyr Gly Val Ser Gly Asn Gln Phe Ile 115 120 125 Lys SerSer Leu Leu Gly Ser Tyr Ser Asp Ile Val Phe 130 135 140 167 348 DNAStreptococcus agalactiae 167 atgaaaggtc tattggattt tttagttaat attgccagaacgccagctat tttagtcgcc 60 ttgatagcca ttatcggttt agtactgcag aaaaaaggtgttcctgatat tgtaaaaggt 120 ggaataaaaa catttgttgg cttcttagtg gtttctgaaggtgcagggat agtccaaaat 180 tccttgaatc catttggaaa aatgtttgaa catgcttttcatttggtggg ggtagttcct 240 aataatgaag ccattgtagc agtagctctt acgaagtatggctcagcaac tgctttgatt 300 atgttagcgg gaatgatttt taatatttta attgctcgttttacaaaa 348 168 116 PRT Streptococcus agalactiae 168 Met Lys Gly LeuLeu Asp Phe Leu Val Asn Ile Ala Arg Thr Pro Ala 1 5 10 15 Ile Leu ValAla Leu Ile Ala Ile Ile Gly Leu Val Leu Gln Lys Lys 20 25 30 Gly Val ProAsp Ile Val Lys Gly Gly Ile Lys Thr Phe Val Gly Phe 35 40 45 Leu Val ValSer Glu Gly Ala Gly Ile Val Gln Asn Ser Leu Asn Pro 50 55 60 Phe Gly LysMet Phe Glu His Ala Phe His Leu Val Gly Val Val Pro 65 70 75 80 Asn AsnGlu Ala Ile Val Ala Val Ala Leu Thr Lys Tyr Gly Ser Ala 85 90 95 Thr AlaLeu Ile Met Leu Ala Gly Met Ile Phe Asn Ile Leu Ile Ala 100 105 110 ArgPhe Thr Lys 115 169 464 DNA Streptococcus agalactiae 169 ttggttggtaagccccaatt actattttta gatgaaccta cttccggaat ggatacttcc 60 acacgtcaacgattttggaa gctggttgcg acactaaaaa aagaaggtga cacaattgtc 120 tattctagtcattatatcga agaggtagaa catacagctg ataggatttt agtacttcat 180 aaaggaaagttattacgcga tacaaccccc tttgccatga agcaagaaaa aaccgaaaag 240 ttattcaccgttccgcttag ttatcaaaaa ttattaccta cctatttgat tacagagtgt 300 gaagccaagagtgatagtat aacgtttgtt actggggagg ctgaaactgt atggaaaata 360 ctggcagataatggttgtcc tattgaagct attgagatga ccaatagaac tttgttaaat 420 cgtatttttgagactactaa ggaggtaaaa catgagaatc ttta 464 170 154 PRT Streptococcusagalactiae 170 Met Val Gly Lys Pro Gln Leu Leu Phe Leu Asp Glu Pro ThrSer Gly 1 5 10 15 Met Asp Thr Ser Thr Arg Gln Arg Phe Trp Lys Leu ValAla Thr Leu 20 25 30 Lys Lys Glu Gly Asp Thr Ile Val Tyr Ser Ser His TyrIle Glu Glu 35 40 45 Val Glu His Thr Ala Asp Arg Ile Leu Val Leu His LysGly Lys Leu 50 55 60 Leu Arg Asp Thr Thr Pro Phe Ala Met Lys Gln Glu LysThr Glu Lys 65 70 75 80 Leu Phe Thr Val Pro Leu Ser Tyr Gln Lys Leu LeuPro Thr Tyr Leu 85 90 95 Ile Thr Glu Cys Glu Ala Lys Ser Asp Ser Ile ThrPhe Val Thr Gly 100 105 110 Glu Ala Glu Thr Val Trp Lys Ile Leu Ala AspAsn Gly Cys Pro Ile 115 120 125 Glu Ala Ile Glu Met Thr Asn Arg Thr LeuLeu Asn Arg Ile Phe Glu 130 135 140 Thr Thr Lys Glu Val Lys His Glu AsnLeu 145 150 171 360 DNA Streptococcus agalactiae 171 ttgaaaaaatccaagagaag ccgtaaggca gtgacaacaa gtggtgagaa gactttactt 60 gaggatttggcaaaaatgaa tttcctagac gaagtcatta atgttatggt tttatatacc 120 ttgaataagacaaaatctgc taacttaaat aaggcctata tcatgaaagt tgctaatgat 180 tttgcctttcagaatgttat gacggccgaa gatgctgtgc ttaaaattcg tgatttttca 240 gatcaaaaagtaaggactaa aacagaaacg aagaagaaac aatcgaatgt tcctgaatgg 300 agtaatcctgattataaaga tgaggttagc ccagaaaaag aaattgaatt agaacagttt 360 172 120 PRTStreptococcus agalactiae 172 Met Lys Lys Ser Lys Arg Ser Arg Lys Ala ValThr Thr Ser Gly Glu 1 5 10 15 Lys Thr Leu Leu Glu Asp Leu Ala Lys MetAsn Phe Leu Asp Glu Val 20 25 30 Ile Asn Val Met Val Leu Tyr Thr Leu AsnLys Thr Lys Ser Ala Asn 35 40 45 Leu Asn Lys Ala Tyr Ile Met Lys Val AlaAsn Asp Phe Ala Phe Gln 50 55 60 Asn Val Met Thr Ala Glu Asp Ala Val LeuLys Ile Arg Asp Phe Ser 65 70 75 80 Asp Gln Lys Val Arg Thr Lys Thr GluThr Lys Lys Lys Gln Ser Asn 85 90 95 Val Pro Glu Trp Ser Asn Pro Asp TyrLys Asp Glu Val Ser Pro Glu 100 105 110 Lys Glu Ile Glu Leu Glu Gln Phe115 120 173 216 DNA Streptococcus agalactiae 173 atgacgaatc atattactaaactgatagaa aatagcggaa aaaaattgac agaaattagc 60 gaagctacag atatagcctatcctacactt tctggataca atcaaggaat ccgcaaacct 120 aaaaaagata atgctgaaaaattggcaaaa tactttaatg tttccgtcgc ttacattatg 180 ggacttgata gcaacccacatgctccatca aatctt 216 174 72 PRT Streptococcus agalactiae 174 Met ThrAsn His Ile Thr Lys Leu Ile Glu Asn Ser Gly Lys Lys Leu 1 5 10 15 ThrGlu Ile Ser Glu Ala Thr Asp Ile Ala Tyr Pro Thr Leu Ser Gly 20 25 30 TyrAsn Gln Gly Ile Arg Lys Pro Lys Lys Asp Asn Ala Glu Lys Leu 35 40 45 AlaLys Tyr Phe Asn Val Ser Val Ala Tyr Ile Met Gly Leu Asp Ser 50 55 60 AsnPro His Ala Pro Ser Asn Leu 65 70 175 337 DNA Streptococcus agalactiae175 ttgatgaaaa ggaataaaca tttaccgtta acagaaacta cctattatat tttattagct 60ttgtttgagg aagcgcatgg ctatgctatt atgaaaaaag ttgaagaaat gagtggcggt 120gatgttagaa tagccgcagg gacaatgtac ggtgccattg aaaatttact taaacaaaaa 180tggataaagt ctatctcaag tgacgataga agaagaaaag tttatattat tactgagaca 240ggaaaagaaa tagtagaact tgaaacgaat cgattaagaa agttacttaa tactgctaat 300cagttgggtt ttggaggaga tggttatgat aaagttt 337 176 112 PRT Streptococcusagalactiae 176 Met Met Lys Arg Asn Lys His Leu Pro Leu Thr Glu Thr ThrTyr Tyr 1 5 10 15 Ile Leu Leu Ala Leu Phe Glu Glu Ala His Gly Tyr AlaIle Met Lys 20 25 30 Lys Val Glu Glu Met Ser Gly Gly Asp Val Arg Ile AlaAla Gly Thr 35 40 45 Met Tyr Gly Ala Ile Glu Asn Leu Leu Lys Gln Lys TrpIle Lys Ser 50 55 60 Ile Ser Ser Asp Asp Arg Arg Arg Lys Val Tyr Ile IleThr Glu Thr 65 70 75 80 Gly Lys Glu Ile Val Glu Leu Glu Thr Asn Arg LeuArg Lys Leu Leu 85 90 95 Asn Thr Ala Asn Gln Leu Gly Phe Gly Gly Asp GlyTyr Asp Lys Val 100 105 110 177 511 DNA Streptococcus agalactiae 177cccattactg gtgagttaat agctgagaaa ttaggagtac caagagcagc actaaggtct 60gatttgcggg ttttaagtat gctaggtatc atagatgcaa aacctaaggt tggttatttt 120tatttaggac agtatcatgc ttcaataggg acaagtcatt ttgaaaagat gacagtttca 180gaaattatgg ggatccttct gacagttcat caaaaagatt cagtttatga tgttattgta 240catattttta tggaagatgc tggttgtgct tttatcttgg atgatgatga ttttctctgt 300ggagtcgtgt cacgtaaaga tttactaaaa accagtattg gcggaggaga tctttctaaa 360atgccaatag gaatggtgat gacacgtatg ccacacgtga caactgtttt agaaaatgaa 420agtctttttg cggcagctga taaattagtg agcagaaaag tggatagtct ccctgtcgtt 480cgtcatgata agcaatatcc cgaaaaattt a 511 178 170 PRT Streptococcusagalactiae 178 Pro Ile Thr Gly Glu Leu Ile Ala Glu Lys Leu Gly Val ProArg Ala 1 5 10 15 Ala Leu Arg Ser Asp Leu Arg Val Leu Ser Met Leu GlyIle Ile Asp 20 25 30 Ala Lys Pro Lys Val Gly Tyr Phe Tyr Leu Gly Gln TyrHis Ala Ser 35 40 45 Ile Gly Thr Ser His Phe Glu Lys Met Thr Val Ser GluIle Met Gly 50 55 60 Ile Leu Leu Thr Val His Gln Lys Asp Ser Val Tyr AspVal Ile Val 65 70 75 80 His Ile Phe Met Glu Asp Ala Gly Cys Ala Phe IleLeu Asp Asp Asp 85 90 95 Asp Phe Leu Cys Gly Val Val Ser Arg Lys Asp LeuLeu Lys Thr Ser 100 105 110 Ile Gly Gly Gly Asp Leu Ser Lys Met Pro IleGly Met Val Met Thr 115 120 125 Arg Met Pro His Val Thr Thr Val Leu GluAsn Glu Ser Leu Phe Ala 130 135 140 Ala Ala Asp Lys Leu Val Ser Arg LysVal Asp Ser Leu Pro Val Val 145 150 155 160 Arg His Asp Lys Gln Tyr ProGlu Lys Phe 165 170 179 233 DNA Streptococcus agalactiae 179 ttggaagtcatcatgcaatt tatttatagt attattggta ttttattggt attaggaatt 60 gtgtatgcaatttctttcaa tcgtaagagt gtttctctaa gtttaattgg aaaagctctt 120 atcgttcaattcattattgc gctaatctta gtacgtatcc cactaggcca acaagttgtt 180 agtgttgtttcaactggagt tactaaagta atcaactgtg gtcaagctgg ttt 233 180 77 PRTStreptococcus agalactiae 180 Met Glu Val Ile Met Gln Phe Ile Tyr Ser IleIle Gly Ile Leu Leu 1 5 10 15 Val Leu Gly Ile Val Tyr Ala Ile Ser PheAsn Arg Lys Ser Val Ser 20 25 30 Leu Ser Leu Ile Gly Lys Ala Leu Ile ValGln Phe Ile Ile Ala Leu 35 40 45 Ile Leu Val Arg Ile Pro Leu Gly Gln GlnVal Val Ser Val Val Ser 50 55 60 Thr Gly Val Thr Lys Val Ile Asn Cys GlyGln Ala Gly 65 70 75 181 344 DNA Streptococcus agalactiae 181 caacctaataaagctttaga aagtgatgag attgatatta atgctttcca gcattataat 60 tacttaaccaattggaataa agcaaataag accaatcttg tttccgttgc tgagacatac 120 tttacttcctttagattata ctctggtact aagaacggta aaggtaaata ccaaacagtt 180 tctgaaattccaaataaagc aactattact atcccaaacg atgcagttaa cgaaagtcgc 240 tctctctacttgttacaatc agcaggcttg ctaaaattga aagtatcagg tgatacatta 300 gcaacaatgtcagatgttgt ttccaatcct aaatctttag attt 344 182 114 PRT Streptococcusagalactiae 182 Gln Pro Asn Lys Ala Leu Glu Ser Asp Glu Ile Asp Ile AsnAla Phe 1 5 10 15 Gln His Tyr Asn Tyr Leu Thr Asn Trp Asn Lys Ala AsnLys Thr Asn 20 25 30 Leu Val Ser Val Ala Glu Thr Tyr Phe Thr Ser Phe ArgLeu Tyr Ser 35 40 45 Gly Thr Lys Asn Gly Lys Gly Lys Tyr Gln Thr Val SerGlu Ile Pro 50 55 60 Asn Lys Ala Thr Ile Thr Ile Pro Asn Asp Ala Val AsnGlu Ser Arg 65 70 75 80 Ser Leu Tyr Leu Leu Gln Ser Ala Gly Leu Leu LysLeu Lys Val Ser 85 90 95 Gly Asp Thr Leu Ala Thr Met Ser Asp Val Val SerAsn Pro Lys Ser 100 105 110 Leu Asp 183 264 DNA Streptococcus agalactiae183 atgaaatgta taataaataa tataaataaa ataaaaatga taattgagat ttatcataga 60aggaaaacta ttttgaaatt aaataaaatc atattatcta ctgcagctct tactgctctc 120tttttaggat ataatagcgt tactgcggat acatataata actatcagcc acatagatca 180aataatatgg atttaactga ggaatataac tataataacc agatagaact tcaggagcgt 240ataaaaaacc taaatatacc tttt 264 184 88 PRT Streptococcus agalactiae 184Met Lys Cys Ile Ile Asn Asn Ile Asn Lys Ile Lys Met Ile Ile Glu 1 5 1015 Ile Tyr His Arg Arg Lys Thr Ile Leu Lys Leu Asn Lys Ile Ile Leu 20 2530 Ser Thr Ala Ala Leu Thr Ala Leu Phe Leu Gly Tyr Asn Ser Val Thr 35 4045 Ala Asp Thr Tyr Asn Asn Tyr Gln Pro His Arg Ser Asn Asn Met Asp 50 5560 Leu Thr Glu Glu Tyr Asn Tyr Asn Asn Gln Ile Glu Leu Gln Glu Arg 65 7075 80 Ile Lys Asn Leu Asn Ile Pro Phe 85 185 926 DNA Streptococcusagalactiae 185 ttgggtgatt attatggtaa gaaatatttt ggtgaggcag ctaaaaaagacgtcgaacat 60 atggctaaga aaatcattaa tgtctataaa acacggttaa aaaacaacacttggttatca 120 gaaaatacaa aagcaatggc cattaagaaa cttgataaca tgagattaatgattggctat 180 ccagaagatt atcctgatct ttatcgtcag taccaatttg atagtaaagcaagcttcttt 240 gaaaacaatg ataactacag aaaattatcg aacaagaaaa catttgaagaatttaaccag 300 tctaatcaac gtgaacattg gcaaatgagt gccaatgctg taaatgcttataatgatcct 360 aataccaatt ccatagtctt tccagcagcg atttttcaat caccactgtacgataaaact 420 aaaacagtta gtcaaaatta tggagctatc ggagcaatta ttggtcatgaaatttcacac 480 tcatttgata ttaatggtat gaaatatgac gagaaaggga atcttcacgattggtggact 540 aaagaagatt taaatcatta taagaaatca acacaagcta tgattgaccaatgggatggc 600 cttaaagcag atggcggtaa agttgatggt aaattaactt tagcagaaaatattgcagat 660 aatggtggtg ttatggcatc tctagaagct cttaagactg aaaaaatccaaactataaag 720 aattttttga atcatgggca agtatttggc gtcaaaaagc aaccaaagaacaaagtaagt 780 cctcaattca gtcagatgtt catgcaccat atgaattgag agctaacatcccagtacgta 840 atttccaaga attttatgat gcctttggtg ttaaaaaagg cgattcaatgtatctaaaac 900 cagaaaaacg tttgacactt tggtaa 926 186 271 PRTStreptococcus agalactiae 186 Met Gly Asp Tyr Tyr Gly Lys Lys Tyr Phe GlyGlu Ala Ala Lys Lys 1 5 10 15 Asp Val Glu His Met Ala Lys Lys Ile IleAsn Val Tyr Lys Thr Arg 20 25 30 Leu Lys Asn Asn Thr Trp Leu Ser Glu AsnThr Lys Ala Met Ala Ile 35 40 45 Lys Lys Leu Asp Asn Met Arg Leu Met IleGly Tyr Pro Asp Tyr Pro 50 55 60 Asp Leu Tyr Arg Gln Tyr Gln Phe Asp SerLys Ala Ser Phe Phe Glu 65 70 75 80 Asn Asn Asp Asn Tyr Arg Lys Leu SerAsn Lys Lys Thr Phe Glu Glu 85 90 95 Phe Asn Gln Ser Asn Gln Arg Glu HisTrp Gln Met Ser Ala Asn Ala 100 105 110 Val Asn Ala Tyr Asn Asp Pro AsnThr Asn Ser Ile Val Phe Pro Ala 115 120 125 Ala Ile Phe Gln Ser Pro LeuTyr Asp Lys Thr Lys Thr Val Ser Gln 130 135 140 Asn Tyr Gly Ala Ile GlyAla Ile Ile Gly His Glu Ile Ser His Ser 145 150 155 160 Phe Asp Ile AsnGly Met Lys Tyr Asp Glu Lys Gly Asn Leu His Asp 165 170 175 Trp Trp ThrLys Glu Asp Leu Asn His Tyr Lys Lys Ser Thr Gln Ala 180 185 190 Met IleAsp Gln Trp Asp Gly Leu Lys Ala Asp Gly Gly Lys Val Asp 195 200 205 GlyLys Leu Thr Leu Ala Glu Asn Ile Ala Asp Asn Gly Gly Val Met 210 215 220Ala Ser Leu Glu Ala Leu Lys Thr Glu Lys Ile Gln Thr Ile Lys Asn 225 230235 240 Phe Leu Asn His Gly Gln Val Phe Gly Val Lys Lys Gln Pro Lys Asn245 250 255 Lys Val Ser Pro Gln Phe Ser Gln Met Phe Met His His Met Asn260 265 270 187 636 DNA Streptococcus agalactiae 187 atgaccatgattacgccaag cttcattaag gtatctctag atgaaacaaa tcgtatgatg 60 cgtatgatatcagatttatt aagtttatcg cgcattgata atgaagtaac gcatttagat 120 gttgaaatgacgaattttac agctttcatg acctcaattt tgaatcgatt tgatcagatt 180 agaaatcaaaaaacagtcac aggaaaagtt tatgaaattg tcagagatta tcctcttaag 240 tcaatttgggtggaaattga tacagataag atgactcaag tgattgataa cattttaaat 300 aatgcagtcaagtattcacc agatggtggt aagattacag ttaatctacg cacaactaaa 360 acgcagatgattttatcaat atcagaccaa ggcttaggta ttcccaaaaa agatttacct 420 ctcatttttgatcgttttta tcgtgttgat aaggcgagaa gtcgtcaaca gggtgggact 480 ggacttggtttgtcaattgc aaaagaaatt gttaagcagc ataagggatt tatttgggct 540 aagagtgagtatggtaaagg gtctactttt acaatcgtct tgccttatga taaagatgct 600 gtaacttatgaagaatggga ggacgttgaa gattaa 636 188 211 PRT Streptococcus agalactiae188 Met Thr Met Ile Thr Pro Ser Phe Ile Lys Val Ser Leu Asp Glu Thr 1 510 15 Asn Arg Met Met Arg Met Ile Ser Asp Leu Leu Ser Leu Ser Arg Ile 2025 30 Asp Asn Glu Val Thr His Leu Asp Val Glu Met Thr Asn Phe Thr Ala 3540 45 Phe Met Thr Ser Ile Leu Asn Arg Phe Asp Gln Ile Arg Asn Gln Lys 5055 60 Thr Val Thr Gly Lys Val Tyr Glu Ile Val Arg Asp Tyr Pro Leu Lys 6570 75 80 Ser Ile Trp Val Glu Ile Asp Thr Asp Lys Met Thr Gln Val Ile Asp85 90 95 Asn Ile Leu Asn Asn Ala Val Lys Tyr Ser Pro Asp Gly Gly Lys Ile100 105 110 Thr Val Asn Leu Arg Thr Thr Lys Thr Gln Met Ile Leu Ser IleSer 115 120 125 Asp Gln Gly Leu Gly Ile Pro Lys Lys Asp Leu Pro Leu IlePhe Asp 130 135 140 Arg Phe Tyr Arg Val Asp Lys Ala Arg Ser Arg Gln GlnGly Gly Thr 145 150 155 160 Gly Leu Gly Leu Ser Ile Ala Lys Glu Ile ValLys Gln His Lys Gly 165 170 175 Phe Ile Trp Ala Lys Ser Glu Tyr Gly LysGly Ser Thr Phe Thr Ile 180 185 190 Val Leu Pro Tyr Asp Lys Asp Ala ValThr Tyr Glu Glu Trp Glu Asp 195 200 205 Val Glu Asp 210 189 1236 DNAStreptococcus agalactiae 189 ttgaaaaaaa ttattacttc tattctatta cttagttgcattttttttat gccaaccatc 60 tctgctgaat cttttaatgc ttccgctaaa catgccttagcagttgattt agattcagga 120 aaaatcttgt atgaaaaaga tgctaacaaa cccgctgctattgcttcctt gactaaaata 180 atgaccgttt atatggtcta taaagaaatt gataacggtaacctcaagtg gaataccaaa 240 gtaaatatat ctgactaccc ttatcaacta acacgcgaatctgatgctag taatgttcct 300 ttagaaaaaa ggcgctatac tgttaaacaa ctcgtggacgctgccatgat ttctagtgct 360 aacagtgcag ccattgcttt agctgaacat atttcaggaactgaaagtaa atttgttgat 420 aaaatgactg ctcaattgga aaagtgggga attcatgatagccacctagt caatgcttct 480 ggcttaaata atagtatgtt aggcaatcac atttatccaaaatcgtcaca aaacgacgaa 540 aataaaatga gtgcacgtga tattgctatt gctgcctaccatttggtcaa cgaatatcct 600 tccattctta agattactag taagtccgtt gctaaatttgataaagatat tatgcattct 660 tataactaca tgctaccaga tatgcctgtc tttagaccaggtattacagg tttgaaaact 720 gggacaacgg aattagctgg ccaatctttt attgctacatctactgaaag tggaatgaga 780 ctactcactg ttattatgca tgctgataag gccgataaagacaaatatgc tcgctttaca 840 gcaactaact ctctcttgaa ctatatcaca aacacctacgaacctaacct tgtattagct 900 aaaggagctg catataaagg taaagaagca agtgtgagagacggaaaaga acaatcggtc 960 atcgctgttg ctaaaaacga tttgaaagta gtacagaagaaaaatatcac taaacaaaat 1020 cagttaaaaa ttaactttaa aaaagagctt actgctcctattacaaaaaa agagaaccta 1080 gggaaagctt attacgttga ccttaataag gttggaaaaggctatctcat aaaggaacct 1140 agcgttcatt tagtggcaaa agatagtatt gagcgcagtttcttcctcaa agtgtggtgg 1200 aatcattttg tgcgctacgt taacgaaaaa ctttaa 1236190 411 PRT Streptococcus agalactiae 190 Met Lys Lys Ile Ile Thr Ser IleLeu Leu Leu Ser Cys Ile Phe Phe 1 5 10 15 Met Pro Thr Ile Ser Ala GluSer Phe Asn Ala Ser Ala Lys His Ala 20 25 30 Leu Ala Val Asp Leu Asp SerGly Lys Ile Leu Tyr Glu Lys Asp Ala 35 40 45 Asn Lys Pro Ala Ala Ile AlaSer Leu Thr Lys Ile Met Thr Val Tyr 50 55 60 Met Val Tyr Lys Glu Ile AspAsn Gly Asn Leu Lys Trp Asn Thr Lys 65 70 75 80 Val Asn Ile Ser Asp TyrPro Tyr Gln Leu Thr Arg Glu Ser Asp Ala 85 90 95 Ser Asn Val Pro Leu GluLys Arg Arg Tyr Thr Val Lys Gln Leu Val 100 105 110 Asp Ala Ala Met IleSer Ser Ala Asn Ser Ala Ala Ile Ala Leu Ala 115 120 125 Glu His Ile SerGly Thr Glu Ser Lys Phe Val Asp Lys Met Thr Ala 130 135 140 Gln Leu GluLys Trp Gly Ile His Asp Ser His Leu Val Asn Ala Ser 145 150 155 160 GlyLeu Asn Asn Ser Met Leu Gly Asn His Ile Tyr Pro Lys Ser Ser 165 170 175Gln Asn Asp Glu Asn Lys Met Ser Ala Arg Asp Ile Ala Ile Ala Ala 180 185190 Tyr His Leu Val Asn Glu Tyr Pro Ser Ile Leu Lys Ile Thr Ser Lys 195200 205 Ser Val Ala Lys Phe Asp Lys Asp Ile Met His Ser Tyr Asn Tyr Met210 215 220 Leu Pro Asp Met Pro Val Phe Arg Pro Gly Ile Thr Gly Leu LysThr 225 230 235 240 Gly Thr Thr Glu Leu Ala Gly Gln Ser Phe Ile Ala ThrSer Thr Glu 245 250 255 Ser Gly Met Arg Leu Leu Thr Val Ile Met His AlaAsp Lys Ala Asp 260 265 270 Lys Asp Lys Tyr Ala Arg Phe Thr Ala Thr AsnSer Leu Leu Asn Tyr 275 280 285 Ile Thr Asn Thr Tyr Glu Pro Asn Leu ValLeu Ala Lys Gly Ala Ala 290 295 300 Tyr Lys Gly Lys Glu Ala Ser Val ArgAsp Gly Lys Glu Gln Ser Val 305 310 315 320 Ile Ala Val Ala Lys Asn AspLeu Lys Val Val Gln Lys Lys Asn Ile 325 330 335 Thr Lys Gln Asn Gln LeuLys Ile Asn Phe Lys Lys Glu Leu Thr Ala 340 345 350 Pro Ile Thr Lys LysGlu Asn Leu Gly Lys Ala Tyr Tyr Val Asp Leu 355 360 365 Asn Lys Val GlyLys Gly Tyr Leu Ile Lys Glu Pro Ser Val His Leu 370 375 380 Val Ala LysAsp Ser Ile Glu Arg Ser Phe Phe Leu Lys Val Trp Trp 385 390 395 400 AsnHis Phe Val Arg Tyr Val Asn Glu Lys Leu 405 410 191 771 DNAStreptococcus agalactiae 191 atgacgcttc gagaattaac aatagaagaa tttaaagaacattcaggaaa ttatgattca 60 caatcatttt tacaaacacc tgagatggct aaacttttagaaaaacgcgg ctatgatgtt 120 aggtatttgg gatatcaagt agaaaataaa ctagagataatcagtttatc ttatattatg 180 ccagtcactg gtggttttca aatgaaaatt gattcaggaccagttcattc aaattctaag 240 tatctaaaac aattttataa agcattgcaa ggctatgccaaatccaacgg tgttctagaa 300 ttaatagttg agccttttga tgattaccaa ttattcactagttcgggagt tcctagtaat 360 cagggaaatg ataatctgat tgaagatttt accagttcaggttatcacca tgatggttta 420 acaactggtt ttactggtaa atatttatct tggcactatgttaaaaattt agaaggtgtc 480 acttctgaaa cgttactatc ttcattctct aagacaggacgagctttggt taagaaagca 540 atgtcttttg gaatcaaggt tcgcgttctt aaacgtgatgagctacattt atttaaagag 600 ataacaactt ctacgtcaaa tagacgtgat tatatggataagtccttaga ttattatcaa 660 gatttttacg atagctttga aggcaaggct gaatttgtgattgccacttt aaattttaga 720 gaatacgacc ataacttgca aataaaagct gaagcattggaaaataagct t 771 192 257 PRT Streptococcus agalactiae 192 Met Thr LeuArg Glu Leu Thr Ile Glu Glu Phe Lys Glu His Ser Gly 1 5 10 15 Asn TyrAsp Ser Gln Ser Phe Leu Gln Thr Pro Glu Met Ala Lys Leu 20 25 30 Leu GluLys Arg Gly Tyr Asp Val Arg Tyr Leu Gly Tyr Gln Val Glu 35 40 45 Asn LysLeu Glu Ile Ile Ser Leu Ser Tyr Ile Met Pro Val Thr Gly 50 55 60 Gly PheGln Met Lys Ile Asp Ser Gly Pro Val His Ser Asn Ser Lys 65 70 75 80 TyrLeu Lys Gln Phe Tyr Lys Ala Leu Gln Gly Tyr Ala Lys Ser Asn 85 90 95 GlyVal Leu Glu Leu Ile Val Glu Pro Phe Asp Asp Tyr Gln Leu Phe 100 105 110Thr Ser Ser Gly Val Pro Ser Asn Gln Gly Asn Asp Asn Leu Ile Glu 115 120125 Asp Phe Thr Ser Ser Gly Tyr His His Asp Gly Leu Thr Thr Gly Phe 130135 140 Thr Gly Lys Tyr Leu Ser Trp His Tyr Val Lys Asn Leu Glu Gly Val145 150 155 160 Thr Ser Glu Thr Leu Leu Ser Ser Phe Ser Lys Thr Gly ArgAla Leu 165 170 175 Val Lys Lys Ala Met Ser Phe Gly Ile Lys Val Arg ValLeu Lys Arg 180 185 190 Asp Glu Leu His Leu Phe Lys Glu Ile Thr Thr SerThr Ser Asn Arg 195 200 205 Arg Asp Tyr Met Asp Lys Ser Leu Asp Tyr TyrGln Asp Phe Tyr Asp 210 215 220 Ser Phe Glu Gly Lys Ala Glu Phe Val IleAla Thr Leu Asn Phe Arg 225 230 235 240 Glu Tyr Asp His Asn Leu Gln IleLys Ala Glu Ala Leu Glu Asn Lys 245 250 255 Leu 193 534 DNAStreptococcus agalactiae 193 ttgtcattaa gtttggttgc agtgttaaat cttatccctcctaaaatcat gggatcagtt 60 attgatgcta ttacaactgg aaaattaaca agaccacaattactatggaa tttattaggt 120 ttggttttgt cagctttagc tatgtatggg ctgcgttatatttggcgtat gtatatttta 180 gggacttctt acaaattagg ccaagttgtc agataccgtttatttgaaca ttttacaaaa 240 atgtctcctt ctttttatca gaaatatcgt acaggtgatttaatggcgca cgcgaccaac 300 gacatcaatt ctctaacacg tcttgcagga ggaggagttatgtcagcagt ggatgcctct 360 atcacagcat tagtaacgct tatcaccatg ttctttactatttcgtggca aatgacatta 420 attgcggtta tccctttgcc cttaatggcc ttagcactagtaaattgggg cgaaaaaccc 480 atgaaacctt caaagaatct caggcagccc ttttcagaattaaataataa agtg 534 194 178 PRT Streptococcus agalactiae 194 Met Ser LeuSer Leu Val Ala Val Leu Asn Leu Ile Pro Pro Lys Ile 1 5 10 15 Met GlySer Val Ile Asp Ala Ile Thr Thr Gly Lys Leu Thr Arg Pro 20 25 30 Gln LeuLeu Trp Asn Leu Leu Gly Leu Val Leu Ser Ala Leu Ala Met 35 40 45 Tyr GlyLeu Arg Tyr Ile Trp Arg Met Tyr Ile Leu Gly Thr Ser Tyr 50 55 60 Lys LeuGly Gln Val Val Arg Tyr Arg Leu Phe Glu His Phe Thr Lys 65 70 75 80 MetSer Pro Ser Phe Tyr Gln Lys Tyr Arg Thr Gly Asp Leu Met Ala 85 90 95 HisAla Thr Asn Asp Ile Asn Ser Leu Thr Arg Leu Ala Gly Gly Gly 100 105 110Val Met Ser Ala Val Asp Ala Ser Ile Thr Ala Leu Val Thr Leu Ile 115 120125 Thr Met Phe Phe Thr Ile Ser Trp Gln Met Thr Leu Ile Ala Val Ile 130135 140 Pro Leu Pro Leu Met Ala Leu Ala Leu Val Asn Trp Gly Glu Lys Pro145 150 155 160 Met Lys Pro Ser Lys Asn Leu Arg Gln Pro Phe Ser Glu LeuAsn Asn 165 170 175 Lys Val 195 440 DNA Streptococcus agalactiae 195atgcatattg agactgttat tgatttcaaa gaattaggaa aaagatatcg ttttaaaaat 60cctacaaaag aattaatagc tgatacttta gaacaagtct tagaagtgat aaaagaagtt 120gattattatc aatctcaaaa ttattatgtt gttggttatt tatcttatga agcatctgct 180gcttttgatt cacattttaa agtttctcaa cagaagttgg ctggagaaca tctagcttat 240tttacagtac ataaagattg tgagaacgaa gcttttcctt taagttatga aaatgttaga 300ttagcagata attggactgc taatgtttct gagcaagaat atcaagaggc aattgctaat 360attaaaggac aaattagaca aggaaatact tatcaagtaa attatacact agagcttagc 420caacaattat gctcggatcc 440 196 146 PRT Streptococcus agalactiae 196 MetHis Ile Glu Thr Val Ile Asp Phe Lys Glu Leu Gly Lys Arg Tyr 1 5 10 15Arg Phe Lys Asn Pro Thr Lys Glu Leu Ile Ala Asp Thr Leu Glu Gln 20 25 30Val Leu Glu Val Ile Lys Glu Val Asp Tyr Tyr Gln Ser Gln Asn Tyr 35 40 45Tyr Val Val Gly Tyr Leu Ser Tyr Glu Ala Ser Ala Ala Phe Asp Ser 50 55 60His Phe Lys Val Ser Gln Gln Lys Leu Ala Gly Glu His Leu Ala Tyr 65 70 7580 Phe Thr Val His Lys Asp Cys Glu Asn Glu Ala Phe Pro Leu Ser Tyr 85 9095 Glu Asn Val Arg Leu Ala Asp Asn Trp Thr Ala Asn Val Ser Glu Gln 100105 110 Glu Tyr Gln Glu Ala Ile Ala Asn Ile Lys Gly Gln Ile Arg Gln Gly115 120 125 Asn Thr Tyr Gln Val Asn Tyr Thr Leu Glu Leu Ser Gln Gln LeuCys 130 135 140 Ser Asp 145 197 1119 DNA Streptococcus agalactiae 197gtgaataata tgttttatct caaaatagcc tggcataatt taaaacattc tatagaccag 60tacataccat tcctcttagc cagtttatta ctttattcat tgacttgttc tacgctacta 120atcttaatga gtgctgttgg aagagatatg gggacagcgg caacggttct ttttcttgga 180gtgattgttt tgtcaatctt tgcggtagtc atggaacatt atagctacaa tatcttgatg 240aaacagcgta gtagtgaatt tggactgtat aacattttgg ggatgaataa acgtcaagtt 300gcgcgtgtag ctagtctaga gctgtttatt atttatatat ttcttatttc tataggaagt 360ctgtttagtg ctttttttgc taaatttatt tatttaattt ttgtcaacat tattaactat 420catgcactaa atcttagttt aagtttatgg ccatttatta tttgtatcgt tatatttaca 480ggtatttttc tgactttaga agttccagtt attcgacatg ttcatttatc atccccatta 540agtcttttta gaaagaaaca acagggagaa aaagaaccaa aaggtaatct tatacttgca 600attttagcgt tagtagctat cgccatcgct tatacaatgg ctcttacttc aggtaaagca 660cctgcattag ctgttatcta tcgtttcttc tttgcagtac ttttagtaat tgctggtact 720tatctttttt atattagttt tatgacatgg tacttaaaaa ggttgcgtca aaacaagcat 780tattattata aatctgagca ttttgtatca acttcgcaaa tgatttttcg aatgaagcaa 840aatgcagtag ggttagcaag tatcacttta ttagctgtta tggctctagt tactattgct 900acaacagtct cactctattc aaatacacaa aatgttgtta ccggactatt tccaaaatca 960gtaagtttat caatagataa ttcaaaaggt gacgcgaaaa atatatttga agaaaagatt 1020ttgaagaaac taggtaagtc atctaaggaa gctatcactt ataatcagac aatgatttcg 1080atgccagtta gtcaatcaag tgacttaata tcacatcta 1119 198 373 PRTStreptococcus agalactiae 198 Met Asn Asn Met Phe Tyr Leu Lys Ile Ala TrpHis Asn Leu Lys His 1 5 10 15 Ser Ile Asp Gln Tyr Ile Pro Phe Leu LeuAla Ser Leu Leu Leu Tyr 20 25 30 Ser Leu Thr Cys Ser Thr Leu Leu Ile LeuMet Ser Ala Val Gly Arg 35 40 45 Asp Met Gly Thr Ala Ala Thr Val Leu PheLeu Gly Val Ile Val Leu 50 55 60 Ser Ile Phe Ala Val Val Met Glu His TyrSer Tyr Asn Ile Leu Met 65 70 75 80 Lys Gln Arg Ser Ser Glu Phe Gly LeuTyr Asn Ile Leu Gly Met Asn 85 90 95 Lys Arg Gln Val Ala Arg Val Ala SerLeu Glu Leu Phe Ile Ile Tyr 100 105 110 Ile Phe Leu Ile Ser Ile Gly SerLeu Phe Ser Ala Phe Phe Ala Lys 115 120 125 Phe Ile Tyr Leu Ile Phe ValAsn Ile Ile Asn Tyr His Ala Leu Asn 130 135 140 Leu Ser Leu Ser Leu TrpPro Phe Ile Ile Cys Ile Val Ile Phe Thr 145 150 155 160 Gly Ile Phe LeuThr Leu Glu Val Pro Val Ile Arg His Val His Leu 165 170 175 Ser Ser ProLeu Ser Leu Phe Arg Lys Lys Gln Gln Gly Glu Lys Glu 180 185 190 Pro LysGly Asn Leu Ile Leu Ala Ile Leu Ala Leu Val Ala Ile Ala 195 200 205 IleAla Tyr Thr Met Ala Leu Thr Ser Gly Lys Ala Pro Ala Leu Ala 210 215 220Val Ile Tyr Arg Phe Phe Phe Ala Val Leu Leu Val Ile Ala Gly Thr 225 230235 240 Tyr Leu Phe Tyr Ile Ser Phe Met Thr Trp Tyr Leu Lys Arg Leu Arg245 250 255 Gln Asn Lys His Tyr Tyr Tyr Lys Ser Glu His Phe Val Ser ThrSer 260 265 270 Gln Met Ile Phe Arg Met Lys Gln Asn Ala Val Gly Leu AlaSer Ile 275 280 285 Thr Leu Leu Ala Val Met Ala Leu Val Thr Ile Ala ThrThr Val Ser 290 295 300 Leu Tyr Ser Asn Thr Gln Asn Val Val Thr Gly LeuPhe Pro Lys Ser 305 310 315 320 Val Ser Leu Ser Ile Asp Asn Ser Lys GlyAsp Ala Lys Asn Ile Phe 325 330 335 Glu Glu Lys Ile Leu Lys Lys Leu GlyLys Ser Ser Lys Glu Ala Ile 340 345 350 Thr Tyr Asn Gln Thr Met Ile SerMet Pro Val Ser Gln Ser Ser Asp 355 360 365 Leu Ile Ser His Leu 370 199735 DNA Streptococcus agalactiae 199 atggttgagc caattatttc aatacaaggacttcataaaa gttttgggaa aaatgaggtt 60 ttaaaaggca ttgacttgga tattcatcaaggagaagtgg tggttattat tggcccttct 120 ggctctggta agtcaacatt tttaagaacaatgaatctct tggaagtacc aacaaaggga 180 acagtgactt ttgaagggat tgatataacagacaaaaaga atgatatttt taaaatgcgc 240 gaaaaaatgg gcatggtttt tcaacagttcaatctatttc ccaatatgac tgtactagaa 300 aatattactt tatcacctat taagacaaagggactttcta agcttgatgc tcagacaaaa 360 gcatacgagc tacttgaaaa agttggactcaaagagaagg ctaatgctta tccagcaagc 420 ttatctggag gacaacaaca acggattgctattgcaagag gtcttgcaat gaatcctgat 480 gtccttcttt ttgatgaacc tacttcagctcttgatcctg aaatggtagg tgaagtcttg 540 actgttatgc aagatttagc taaatctggtatgacgatgg ttattgtcac tcatgaaatg 600 ggttttgcac gtgaagtagc ggatcgtgtcatttttatgg atgcagggat tattgttgag 660 caagggaccc ctaagaaagt atttgagcagacaaaagaaa tccgcacaag agacttctta 720 agtaaagtat tataa 735 200 244 PRTStreptococcus agalactiae 200 Met Val Glu Pro Ile Ile Ser Ile Gln Gly LeuHis Lys Ser Phe Gly 1 5 10 15 Lys Asn Glu Val Leu Lys Gly Ile Asp LeuAsp Ile His Gln Gly Glu 20 25 30 Val Val Val Ile Ile Gly Pro Ser Gly SerGly Lys Ser Thr Phe Leu 35 40 45 Arg Thr Met Asn Leu Leu Glu Val Pro ThrLys Gly Thr Val Thr Phe 50 55 60 Glu Gly Ile Asp Ile Thr Asp Lys Lys AsnAsp Ile Phe Lys Met Arg 65 70 75 80 Glu Lys Met Gly Met Val Phe Gln GlnPhe Asn Leu Phe Pro Asn Met 85 90 95 Thr Val Leu Glu Asn Ile Thr Leu SerPro Ile Lys Thr Lys Gly Leu 100 105 110 Ser Lys Leu Asp Ala Gln Thr LysAla Tyr Glu Leu Leu Glu Lys Val 115 120 125 Gly Leu Lys Glu Lys Ala AsnAla Tyr Pro Ala Ser Leu Ser Gly Gly 130 135 140 Gln Gln Gln Arg Ile AlaIle Ala Arg Gly Leu Ala Met Asn Pro Asp 145 150 155 160 Val Leu Leu PheAsp Glu Pro Thr Ser Ala Leu Asp Pro Glu Met Val 165 170 175 Gly Glu ValLeu Thr Val Met Gln Asp Leu Ala Lys Ser Gly Met Thr 180 185 190 Met ValIle Val Thr His Glu Met Gly Phe Ala Arg Glu Val Ala Asp 195 200 205 ArgVal Ile Phe Met Asp Ala Gly Ile Ile Val Glu Gln Gly Thr Pro 210 215 220Lys Lys Val Phe Glu Gln Thr Lys Glu Ile Arg Thr Arg Asp Phe Leu 225 230235 240 Ser Lys Val Leu 201 348 DNA Streptococcus agalactiae 201atgtctcast atcaagagtg gttagaaaac gactcactcg gtaaagatat taagtcagat 60ttagaagcta ttaaaggaga tgaatctgaa attcaggatc gtttttacaa aacattagaa 120tttggaacgg cgggattgag aggtaaactt ggagcaggaa ccaatcgtat gaatacttat 180atggtgggga aagcagcaca agcattagct aatcgattat tgatcatggc cctgaagcta 240ttgcacgtgg aattgcagtt agttatgatg tcccgttatc aatctaagga atttgcagaa 300ttaacttggt ccattatggc agcaaatggt attaaagcct tatattta 348 202 122 PRTStreptococcus agalactiae 202 Met Ser His Met Asn Tyr Lys Glu Ile Tyr GlnGlu Trp Leu Glu Asn 1 5 10 15 Asp Ser Leu Gly Lys Asp Ile Lys Ser AspLeu Glu Ala Ile Lys Gly 20 25 30 Asp Glu Ser Glu Ile Gln Asp Arg Phe TyrLys Thr Leu Glu Phe Gly 35 40 45 Thr Ala Gly Leu Arg Gly Lys Leu Gly AlaGly Thr Asn Arg Met Asn 50 55 60 Thr Tyr Met Val Gly Lys Ala Ala Gln AlaLeu Ala Asn Arg Leu Leu 65 70 75 80 Ile Met Ala Leu Lys Leu Leu His ValGlu Leu Gln Leu Val Met Met 85 90 95 Ser Arg Tyr Gln Ser Lys Glu Phe AlaGlu Leu Thr Trp Ser Ile Met 100 105 110 Ala Ala Asn Gly Ile Lys Ala LeuTyr Leu 115 120 203 1068 DNA Streptococcus agalactiae 203 atgcaacctgtaaaagtcga tgaaccttct gttgaagaaa ccattactat tttgaaaggt 60 atccaaaaaaaatacgaaga ttatcatcac gtaaaatata ataatgatgc catagaagca 120 gctgcagtactatctaatcg ttatatccaa gaccgctttt tacctgataa agcaatagac 180 ttattagatgaagctggttc taaaatgaac ctaacactaa attttgttga tccaaaagaa 240 attgatcaacgtctcattga agcagaaaat ttaaaagcgc aagcgactcg tgaagaagat 300 tacgaacgtgcagcttactt ccgtgaccag attgcaaaat ataaagaaat gcagcaacaa 360 aaggtcgacgatcaagatac acctattatt accgaaaaaa caattgagca catcattgaa 420 gaaaaaacgaatatccctgt tggtgattta aaagaaaaag aacaatctca attaattaat 480 ctcgcagatgacttgaaaca gcatgtgatc ggccaggatg acgctgtcat taagattgca 540 aaagctattcgtcgtaatcg agttggtctt ggtagcccaa accgtcctat tggttccttt 600 ttatttgtaggaccaaccgg tgttggtaaa actgaacttt ctaaacaact agcaattgag 660 ctctttggttcagctgatag tatgattcgt tttgatatgt cagagtacat ggaaaagcat 720 gctgttgctaaattagtcgg agcgcctcca ggatacgtgg gatacgagga agctggacaa 780 ctaactgaaaaggttcgtcg aaatccttac tcgctcatcc ttctagatga aattgaaaaa 840 gctcatcccgatgtcatgca tatgttcttg caggtccttg atgacggtcg attaacagat 900 ggacaaggaagaactgttag ttttaaagat accattatca tcatgacctc aaatgctggt 960 tctggtaaaactgaagcaag tgttggcttt ggtgcctcac gagaaggtag gacgaattcg 1020 agctcggtacccggggatcc tctagagtcg acctgcaggc atgcaagc 1068 204 356 PRT Streptococcusagalactiae 204 Met Gln Pro Val Lys Val Asp Glu Pro Ser Val Glu Glu ThrIle Thr 1 5 10 15 Ile Leu Lys Gly Ile Gln Lys Lys Tyr Glu Asp Tyr HisHis Val Lys 20 25 30 Tyr Asn Asn Asp Ala Ile Glu Ala Ala Ala Val Leu SerAsn Arg Tyr 35 40 45 Ile Gln Asp Arg Phe Leu Pro Asp Lys Ala Ile Asp LeuLeu Asp Glu 50 55 60 Ala Gly Ser Lys Met Asn Leu Thr Leu Asn Phe Val AspPro Lys Glu 65 70 75 80 Ile Asp Gln Arg Leu Ile Glu Ala Glu Asn Leu LysAla Gln Ala Thr 85 90 95 Arg Glu Glu Asp Tyr Glu Arg Ala Ala Tyr Phe ArgAsp Gln Ile Ala 100 105 110 Lys Tyr Lys Glu Met Gln Gln Gln Lys Val AspAsp Gln Asp Thr Pro 115 120 125 Ile Ile Thr Glu Lys Thr Ile Glu His IleIle Glu Glu Lys Thr Asn 130 135 140 Ile Pro Val Gly Asp Leu Lys Glu LysGlu Gln Ser Gln Leu Ile Asn 145 150 155 160 Leu Ala Asp Asp Leu Lys GlnHis Val Ile Gly Gln Asp Asp Ala Val 165 170 175 Ile Lys Ile Ala Lys AlaIle Arg Arg Asn Arg Val Gly Leu Gly Ser 180 185 190 Pro Asn Arg Pro IleGly Ser Phe Leu Phe Val Gly Pro Thr Gly Val 195 200 205 Gly Lys Thr GluLeu Ser Lys Gln Leu Ala Ile Glu Leu Phe Gly Ser 210 215 220 Ala Asp SerMet Ile Arg Phe Asp Met Ser Glu Tyr Met Glu Lys His 225 230 235 240 AlaVal Ala Lys Leu Val Gly Ala Pro Pro Gly Tyr Val Gly Tyr Glu 245 250 255Glu Ala Gly Gln Leu Thr Glu Lys Val Arg Arg Asn Pro Tyr Ser Leu 260 265270 Ile Leu Leu Asp Glu Ile Glu Lys Ala His Pro Asp Val Met His Met 275280 285 Phe Leu Gln Val Leu Asp Asp Gly Arg Leu Thr Asp Gly Gln Gly Arg290 295 300 Thr Val Ser Phe Lys Asp Thr Ile Ile Ile Met Thr Ser Asn AlaGly 305 310 315 320 Ser Gly Lys Thr Glu Ala Ser Val Gly Phe Gly Ala SerArg Glu Gly 325 330 335 Arg Thr Asn Ser Ser Ser Val Pro Gly Asp Pro LeuGlu Ser Thr Cys 340 345 350 Arg His Ala Ser 355 205 582 DNAStreptococcus agalactiae 205 atgagaggga aggttattta cggcacaacc cttataggtctttttctatt cttatttttc 60 tatttttgga ttcctaagca tcacatcgag agaatacatcatcatcgtat aaagcaggta 120 gatgcgaaga gtgatttaac aggatttaaa acccatttgcccattatcag cattgataca 180 aagcaacaag ttattcctct tgttacaaaa gaaggcggaaaatatgtcaa agctagggat 240 aatattaatg ttgatatcga attacgggat tctccaagtagatcacatca tttatcagaa 300 aagccgagaa ttaggacaaa agggttaata tcatatagaggaaattcctc tcgttacttt 360 gataagaagt cattgaaagt taagtttgtt actaataagttaaaggaaaa gaagcatcga 420 ttagcaggaa tgcctaaaga atcggagtgg gtattgcatggtccctttct agacagaaca 480 ttattaagaa attatctgag ttataatatt gctggtgagattatgcctat gccccaaacg 540 ttcgctactg tgagttattt gtcaatggtg agtatcaggg ag582 206 194 PRT Streptococcus agalactiae 206 Met Arg Gly Lys Val Ile TyrGly Thr Thr Leu Ile Gly Leu Phe Leu 1 5 10 15 Phe Leu Phe Phe Tyr PheTrp Ile Pro Lys His His Ile Glu Arg Ile 20 25 30 His His His Arg Ile LysGln Val Asp Ala Lys Ser Asp Leu Thr Gly 35 40 45 Phe Lys Thr His Leu ProIle Ile Ser Ile Asp Thr Lys Gln Gln Val 50 55 60 Ile Pro Leu Val Thr LysGlu Gly Gly Lys Tyr Val Lys Ala Arg Asp 65 70 75 80 Asn Ile Asn Val AspIle Glu Leu Arg Asp Ser Pro Ser Arg Ser His 85 90 95 His Leu Ser Glu LysPro Arg Ile Arg Thr Lys Gly Leu Ile Ser Tyr 100 105 110 Arg Gly Asn SerSer Arg Tyr Phe Asp Lys Lys Ser Leu Lys Val Lys 115 120 125 Phe Val ThrAsn Lys Leu Lys Glu Lys Lys His Arg Leu Ala Gly Met 130 135 140 Pro LysGlu Ser Glu Trp Val Leu His Gly Pro Phe Leu Asp Arg Thr 145 150 155 160Leu Leu Arg Asn Tyr Leu Ser Tyr Asn Ile Ala Gly Glu Ile Met Pro 165 170175 Met Pro Gln Thr Phe Ala Thr Val Ser Tyr Leu Ser Met Val Ser Ile 180185 190 Arg Glu 207 498 DNA Streptococcus agalactiae 207 cttcacattttattgatcac tatctgacaa atgttaatca aacagcagtt cttattttag 60 tgggatattattcaatgtat gtcttgcaga ccttaattca atattttggg aatctctttt 120 ttgcgcgtgtttcttatagt attgttagag atattcgtag agatgctttt gctaatatgg 180 aaaggctaggcatgtcttat tttgatagga caccggcagg atctattgtg tcacgtatta 240 ctaatgatactgaagcaata tctgatatgt tttcgggtat tttatcaagt tttatctcgg 300 cgatatttatttttacagtt actctgtaca ctatgttgat gctagacatt aaactaacag 360 gactcgtcgctcttttgtta cctgttatct ttatattagt gaatgtctat cggaaaaaat 420 cagtcactgtcattgctaaa acgagaagtt tacttagtga tatcaacagt aaattatcag 480 aaagtattgaaggaattc 498 208 165 PRT Streptococcus agalactiae 208 Ser His Phe IleAsp His Tyr Leu Thr Asn Val Asn Gln Thr Ala Val 1 5 10 15 Leu Ile LeuVal Gly Tyr Tyr Ser Met Tyr Val Leu Gln Thr Leu Ile 20 25 30 Gln Tyr PheGly Asn Leu Phe Phe Ala Arg Val Ser Tyr Ser Ile Val 35 40 45 Arg Asp IleArg Arg Asp Ala Phe Ala Asn Met Glu Arg Leu Gly Met 50 55 60 Ser Tyr PheAsp Arg Thr Pro Ala Gly Ser Ile Val Ser Arg Ile Thr 65 70 75 80 Asn AspThr Glu Ala Ile Ser Asp Met Phe Ser Gly Ile Leu Ser Ser 85 90 95 Phe IleSer Ala Ile Phe Ile Phe Thr Val Thr Leu Tyr Thr Met Leu 100 105 110 MetLeu Asp Ile Lys Leu Thr Gly Leu Val Ala Leu Leu Leu Pro Val 115 120 125Ile Phe Ile Leu Val Asn Val Tyr Arg Lys Lys Ser Val Thr Val Ile 130 135140 Ala Lys Thr Arg Ser Leu Leu Ser Asp Ile Asn Ser Lys Leu Ser Glu 145150 155 160 Ser Ile Glu Gly Ile 165 209 681 DNA Streptococcus agalactiae209 atgtaccata ttgaattaaa aaaggaagct ttactaccaa gagaacgcct agttgattta 60ggcgcagata gattgagtaa tcaggagtta ttagccattc tcttacgtac aggtattaaa 120gaaaaacctg ttcttgaaat ttcaacgcaa attttagaaa acataagcag tttagcagat 180tttggtcaat tatccttaca ggagttgcaa tccattaaag gaatcggtca ggttaaatcc 240gtcgaaataa aagctatgct agaactagca aaacggattc acaaagctga atatgatcgt 300aaagagcaaa ttttaagtag tgaacaatta gcgaggaaaa tgatgctcga attaggggat 360aaaaaacaag aacatttagt agctatttat atggatacac aaaatcgtat tatcgaacag 420agaactattt ttattggtac tgtacgtcgt tcagtagcag agccaagaga aattctacat 480tatgcttgta aaaacatggc aacttctttg attattatac ataatcatcc ctcaggttct 540ccaaatccca gtgaaagtga tttaagtttc actaaaaaaa taaaacgatc atgtgatcat 600ctgggaattg tctgcctaga tcacatcatc gttggaaaaa ataaatatta tagttttcga 660gaagaagcag atattttata a 681 210 226 PRT Streptococcus agalactiae 210 MetTyr His Ile Glu Leu Lys Lys Glu Ala Leu Leu Pro Arg Glu Arg 1 5 10 15Leu Val Asp Leu Gly Ala Asp Arg Leu Ser Asn Gln Glu Leu Leu Ala 20 25 30Ile Leu Leu Arg Thr Gly Ile Lys Glu Lys Pro Val Leu Glu Ile Ser 35 40 45Thr Gln Ile Leu Glu Asn Ile Ser Ser Leu Ala Asp Phe Gly Gln Leu 50 55 60Ser Leu Gln Glu Leu Gln Ser Ile Lys Gly Ile Gly Gln Val Lys Ser 65 70 7580 Val Glu Ile Lys Ala Met Leu Glu Leu Ala Lys Arg Ile His Lys Ala 85 9095 Glu Tyr Asp Arg Lys Glu Gln Ile Leu Ser Ser Glu Gln Leu Ala Arg 100105 110 Lys Met Met Leu Glu Leu Gly Asp Lys Lys Gln Glu His Leu Val Ala115 120 125 Ile Tyr Met Asp Thr Gln Asn Arg Ile Ile Glu Gln Arg Thr IlePhe 130 135 140 Ile Gly Thr Val Arg Arg Ser Val Ala Glu Pro Arg Glu IleLeu His 145 150 155 160 Tyr Ala Cys Lys Asn Met Ala Thr Ser Leu Ile IleIle His Asn His 165 170 175 Pro Ser Gly Ser Pro Asn Pro Ser Glu Ser AspLeu Ser Phe Thr Lys 180 185 190 Lys Ile Lys Arg Ser Cys Asp His Leu GlyIle Val Cys Leu Asp His 195 200 205 Ile Ile Val Gly Lys Asn Lys Tyr TyrSer Phe Arg Glu Glu Ala Asp 210 215 220 Ile Leu 225 211 579 DNAStreptococcus agalactiae 211 tggttaaaag tagtgatagc ttgtattcca tctattttaattgctttacc atttgataat 60 tggtttgaag ctcattttaa tttcatgatt ccgattgcaatagccctaat cttttatggt 120 tttgtcttca tatgggttga aaaacgtaat gcacacctcaaaccacaggt aaccgaattg 180 gcaagtatgt cttacaagac agctttcttg attggatgtttccaggttct cagtattgtt 240 ccgggaacca gtcgttctgg agctactatt ttaggagcaattattattgg aactagtcgt 300 tcggtcgctg ctgactttac tttcttcctt gccatcccaactatgtttgg ttatagtgga 360 cttaaggcgg ttaaatattt tttagatggt aacgtcttgagtttagacca atctttaata 420 cttttagtag caagtctgac agctttcgta gttagtttatatgttattcg tttcttgaca 480 gactatgtca aacgacacga tttcaccatc tttggtaagtatcgtatagt cttaggaagt 540 ttactcatcc tctactggtt agttgttcat ttattctaa 579212 192 PRT Streptococcus agalactiae 212 Trp Leu Lys Val Val Ile Ala CysIle Pro Ser Ile Leu Ile Ala Leu 1 5 10 15 Pro Phe Asp Asn Trp Phe GluAla His Phe Asn Phe Met Ile Pro Ile 20 25 30 Ala Ile Ala Leu Ile Phe TyrGly Phe Val Phe Ile Trp Val Glu Lys 35 40 45 Arg Asn Ala His Leu Lys ProGln Val Thr Glu Leu Ala Ser Met Ser 50 55 60 Tyr Lys Thr Ala Phe Leu IleGly Cys Phe Gln Val Leu Ser Ile Val 65 70 75 80 Pro Gly Thr Ser Arg SerGly Ala Thr Ile Leu Gly Ala Ile Ile Ile 85 90 95 Gly Thr Ser Arg Ser ValAla Ala Asp Phe Thr Phe Phe Leu Ala Ile 100 105 110 Pro Thr Met Phe GlyTyr Ser Gly Leu Lys Ala Val Lys Tyr Phe Leu 115 120 125 Asp Gly Asn ValLeu Ser Leu Asp Gln Ser Leu Ile Leu Leu Val Ala 130 135 140 Ser Leu ThrAla Phe Val Val Ser Leu Tyr Val Ile Arg Phe Leu Thr 145 150 155 160 AspTyr Val Lys Arg His Asp Phe Thr Ile Phe Gly Lys Tyr Arg Ile 165 170 175Val Leu Gly Ser Leu Leu Ile Leu Tyr Trp Leu Val Val His Leu Phe 180 185190 213 547 DNA Streptococcus agalactiae 213 atggaaatga aacaaatcagtgaaacaaca ctgaaaatta caattagtat ggaagattta 60 gaagatcgtg gtatggagctgaaagatttc ctaatccctc aggagaagac tgaggaattt 120 ttctattctg tcatggatgaattagacttg ccagaaaact ttaaaaatag tggtatgtta 180 agttttcgag taacacctaaaaaagatcgc attgatgttt ttgttacaaa gtctgaatta 240 agtaaagatt taaatttagaagaattagca gatttgggtg acatttcaaa aatgtctcca 300 gaagactttt ttaaaaccttggaacaatcg atgttggaaa aaggggatac ggatgcccat 360 gccaaattag cagaaattgaaaatatgatg gataaagcaa ctcaagaagt agttgaggaa 420 aatgtttctg aagaacaacctgaaaaggaa gtagaaacga ttggatatgt tcactatgtc 480 tttgattttg ataatattgaagctgtagtt cgattttcac aaacgattga ttttccaata 540 gaagctt 547 214 182 PRTStreptococcus agalactiae 214 Met Glu Met Lys Gln Ile Ser Glu Thr Thr LeuLys Ile Thr Ile Ser 1 5 10 15 Met Glu Asp Leu Glu Asp Arg Gly Met GluLeu Lys Asp Phe Leu Ile 20 25 30 Pro Gln Glu Lys Thr Glu Glu Phe Phe TyrSer Val Met Asp Glu Leu 35 40 45 Asp Leu Pro Glu Asn Phe Lys Asn Ser GlyMet Leu Ser Phe Arg Val 50 55 60 Thr Pro Lys Lys Asp Arg Ile Asp Val PheVal Thr Lys Ser Glu Leu 65 70 75 80 Ser Lys Asp Leu Asn Leu Glu Glu LeuAla Asp Leu Gly Asp Ile Ser 85 90 95 Lys Met Ser Pro Glu Asp Phe Phe LysThr Leu Glu Gln Ser Met Leu 100 105 110 Glu Lys Gly Asp Thr Asp Ala HisAla Lys Leu Ala Glu Ile Glu Asn 115 120 125 Met Met Asp Lys Ala Thr GlnGlu Val Val Glu Glu Asn Val Ser Glu 130 135 140 Glu Gln Pro Glu Lys GluVal Glu Thr Ile Gly Tyr Val His Tyr Val 145 150 155 160 Phe Asp Phe AspAsn Ile Glu Ala Val Val Arg Phe Ser Gln Thr Ile 165 170 175 Asp Phe ProIle Glu Ala 180 215 447 DNA Streptococcus agalactiae 215 ggaaaccaacggccagtaca atcgtcaagg gtagattatc ctaaacgtag tcgtgccaag 60 attgtagaagtttattttag acaagcttct actactgatt attctggtgt ttacaaaggt 120 tactatattgactttgaagc caaagaaacc cggcagaaaa ctgctatgcc tatgaaaaat 180 tttcatgctcaccaaataga gcacatggca aatgtattac agcaaaaagg gatttgcttt 240 gtcttgcttcatttttccac acttaaggaa acctatctac tccctgctaa tgagttaatt 300 tcattttatcagattgataa aggcaataaa tcaatgccta ttgattatat cagaaaaaat 360 ggatttttcgtaaaggagag tgcctttcct caagtccctt acttagatat tattgaagaa 420 aaattattaggcggtgatta caattaa 447 216 148 PRT Streptococcus agalactiae 216 Gly AsnGln Arg Pro Val Gln Ser Ser Arg Val Asp Tyr Pro Lys Arg 1 5 10 15 SerArg Ala Lys Ile Val Glu Val Tyr Phe Arg Gln Ala Ser Thr Thr 20 25 30 AspTyr Ser Gly Val Tyr Lys Gly Tyr Tyr Ile Asp Phe Glu Ala Lys 35 40 45 GluThr Arg Gln Lys Thr Ala Met Pro Met Lys Asn Phe His Ala His 50 55 60 GlnIle Glu His Met Ala Asn Val Leu Gln Gln Lys Gly Ile Cys Phe 65 70 75 80Val Leu Leu His Phe Ser Thr Leu Lys Glu Thr Tyr Leu Leu Pro Ala 85 90 95Asn Glu Leu Ile Ser Phe Tyr Gln Ile Asp Lys Gly Asn Lys Ser Met 100 105110 Pro Ile Asp Tyr Ile Arg Lys Asn Gly Phe Phe Val Lys Glu Ser Ala 115120 125 Phe Pro Gln Val Pro Tyr Leu Asp Ile Ile Glu Glu Lys Leu Leu Gly130 135 140 Gly Asp Tyr Asn 145 217 433 DNA Streptococcus agalactiae 217ggatcctaaa aacgctaagg tttatcaaaa aaatgctgat caatttagtg acaaggcaat 60ggctattgca gagaagtata agccaaaatt taaagctgca aagtctaaat actttgtgac 120ttcacataca gcattctcat acttagctaa gcgatacgga ttgactcagt taggtattgc 180aggtgtctca accgagcaag aacctagtgc taaaaaatta gccgaaattc aggagtttgt 240gaaaacatat aaggttaaga ctatttttgt tgaagaagga gtctcaccta aattagctca 300agcagtagct tcagctactc gagttaaaat tgcaagttta agtcctttag aagcagttcc 360caaaaacaat aaagattact tagaaaattt ggaaactaat cttaaggtac ttgtcaaatc 420gttaaatcaa tag 433 218 143 PRT Streptococcus agalactiae 218 Asp Pro LysAsn Ala Lys Val Tyr Gln Lys Asn Ala Asp Gln Phe Ser 1 5 10 15 Asp LysAla Met Ala Ile Ala Glu Lys Tyr Lys Pro Lys Phe Lys Ala 20 25 30 Ala LysSer Lys Tyr Phe Val Thr Ser His Thr Ala Phe Ser Tyr Leu 35 40 45 Ala LysArg Tyr Gly Leu Thr Gln Leu Gly Ile Ala Gly Val Ser Thr 50 55 60 Glu GlnGlu Pro Ser Ala Lys Lys Leu Ala Glu Ile Gln Glu Phe Val 65 70 75 80 LysThr Tyr Lys Val Lys Thr Ile Phe Val Glu Glu Gly Val Ser Pro 85 90 95 LysLeu Ala Gln Ala Val Ala Ser Ala Thr Arg Val Lys Ile Ala Ser 100 105 110Leu Ser Pro Leu Glu Ala Val Pro Lys Asn Asn Lys Asp Tyr Leu Glu 115 120125 Asn Leu Glu Thr Asn Leu Lys Val Leu Val Lys Ser Leu Asn Gln 130 135140 219 717 DNA Streptococcus agalactiae 219 atgaaaaaag tcatcgatttaaaaaaacta caaaaagcat acgcctcaga aactgtttta 60 aataatatta atttggaggtgtttaaagga gaaataattg gattaatagg accctctgga 120 gcagggaaat ctaccttgattaaaactatg cttggcatgg aaaaagcaga taagggaaca 180 gctcttgttc ttgatactcaaatgccagat cgtaatattt taaatcaaat tggctatatg 240 gctcaatctg atgccttacacgagtcttta actggcttag aaaatttatt attctttgga 300 aaaatgaaag gtattcaaaaaactgaatta aaacagcaga taactcatat ttctaaagta 360 gtagatctag aaaaccaacttgataaattt gtctcaggtt actcagaagg tatgaaaaga 420 cggctttctc tagccatcgccctacttgga aaccccacag ttttaatcct agatgaacct 480 accgttggaa ttgatccatccttgaggaga aaaatctggc aagagctaat taatattaag 540 gatgaaggac gttctatctttattacaacc cacgttatgg atgaagcaga attaacaagt 600 aaggttgcac tactattacgtggaaacatt attgcctttg atactccatt acatttaaaa 660 aaacaattta atgtgagtactattgaggaa gttttcttaa aagctgaagg agaataa 717 220 238 PRT Streptococcusagalactiae 220 Met Lys Lys Val Ile Asp Leu Lys Lys Leu Gln Lys Ala TyrAla Ser 1 5 10 15 Glu Thr Val Leu Asn Asn Ile Asn Leu Glu Val Phe LysGly Glu Ile 20 25 30 Ile Gly Leu Ile Gly Pro Ser Gly Ala Gly Lys Ser ThrLeu Ile Lys 35 40 45 Thr Met Leu Gly Met Glu Lys Ala Asp Lys Gly Thr AlaLeu Val Leu 50 55 60 Asp Thr Gln Met Pro Asp Arg Asn Ile Leu Asn Gln IleGly Tyr Met 65 70 75 80 Ala Gln Ser Asp Ala Leu His Glu Ser Leu Thr GlyLeu Glu Asn Leu 85 90 95 Leu Phe Phe Gly Lys Met Lys Gly Ile Gln Lys ThrGlu Leu Lys Gln 100 105 110 Gln Ile Thr His Ile Ser Lys Val Val Asp LeuGlu Asn Gln Leu Asp 115 120 125 Lys Phe Val Ser Gly Tyr Ser Glu Gly MetLys Arg Arg Leu Ser Leu 130 135 140 Ala Ile Ala Leu Leu Gly Asn Pro ThrVal Leu Ile Leu Asp Glu Pro 145 150 155 160 Thr Val Gly Ile Asp Pro SerLeu Arg Arg Lys Ile Trp Gln Glu Leu 165 170 175 Ile Asn Ile Lys Asp GluGly Arg Ser Ile Phe Ile Thr Thr His Val 180 185 190 Met Asp Glu Ala GluLeu Thr Ser Lys Val Ala Leu Leu Leu Arg Gly 195 200 205 Asn Ile Ile AlaPhe Asp Thr Pro Leu His Leu Lys Lys Gln Phe Asn 210 215 220 Val Ser ThrIle Glu Glu Val Phe Leu Lys Ala Glu Gly Glu 225 230 235 221 591 DNAStreptococcus agalactiae 221 atggtacaaa tgatacatga tatgattaaa acaattgagcattttgctga gacacaagct 60 gattttccag tgtatgatat tttaggggaa gtccatacttatggacaact taaagtagac 120 tctgactctc tagctgctca tattgatagc ctaggccttgttgaaaaatc acctgtctta 180 gtattcggtg gtcaagaata tgaaatgttg gcgacatttgttgctttaac aaagtcaggg 240 catgcttata taccggttga ccaacactct gctttggatagaatacaggc tattatgaca 300 gttgctcaac caagccttat catttcaatt ggtgaatttcctcttgaagt tgataatgtc 360 ccaatcctag acgtttctca agtttcagct atttttgaagaaaagactcc ttatgaggta 420 acacattctg ttaaaggtga tgataattac tatattattttcacttcagg gactactggt 480 ttaccaaaag gtgtgcaaat ttcacatgac aatttattgagctttacaaa ttggatgatt 540 tctgatgatg agttttcagt tcctgaaaga ccgcaaatgttggctcaacc c 591 222 197 PRT Streptococcus agalactiae 222 Met Val GlnMet Ile His Asp Met Ile Lys Thr Ile Glu His Phe Ala 1 5 10 15 Glu ThrGln Ala Asp Phe Pro Val Tyr Asp Ile Leu Gly Glu Val His 20 25 30 Thr TyrGly Gln Leu Lys Val Asp Ser Asp Ser Leu Ala Ala His Ile 35 40 45 Asp SerLeu Gly Leu Val Glu Lys Ser Pro Val Leu Val Phe Gly Gly 50 55 60 Gln GluTyr Glu Met Leu Ala Thr Phe Val Ala Leu Thr Lys Ser Gly 65 70 75 80 HisAla Tyr Ile Pro Val Asp Gln His Ser Ala Leu Asp Arg Ile Gln 85 90 95 AlaIle Met Thr Val Ala Gln Pro Ser Leu Ile Ile Ser Ile Gly Glu 100 105 110Phe Pro Leu Glu Val Asp Asn Val Pro Ile Leu Asp Val Ser Gln Val 115 120125 Ser Ala Ile Phe Glu Glu Lys Thr Pro Tyr Glu Val Thr His Ser Val 130135 140 Lys Gly Asp Asp Asn Tyr Tyr Ile Ile Phe Thr Ser Gly Thr Thr Gly145 150 155 160 Leu Pro Lys Gly Val Gln Ile Ser His Asp Asn Leu Leu SerPhe Thr 165 170 175 Asn Trp Met Ile Ser Asp Asp Glu Phe Ser Val Pro GluArg Pro Gln 180 185 190 Met Leu Ala Gln Pro 195 223 1179 DNAStreptococcus agalactiae 223 atggaaaatc atcgttatga agatgaaggt aaattccagcgtaagatgac cagtcgtcat 60 ctctttatgt tatcgctagg tggtgttatc gggactgggcttttcttgag ttcaggttat 120 accattgcac aggctggtcc gcttggagct gtgctgtcttatttgattgg tgccgttgtg 180 gtttatttgg tcatgctatc acttggggaa ttggcggttgccatgccggt gacggggtca 240 ttccacactt atgccactaa gtttatcagt cctggaacaggttttactgt tgcttggcta 300 tattggattt gttggacggt cgccttgggg actgaatttttaggtgctgc catgctgatg 360 cagcgctggt tcccaaatgt gccggcttgg gcatttgcttccttttttgc ccttgtgatt 420 tttggtttaa atgctcttag cgtacgcttt tttgcagaagcagagtcttt cttctcaagt 480 attaaggtta ttgctatcat tatctttatt atcttgggcttaggtgctat gtttggtcta 540 gtttcctttg aaggtcagca caaggctatt ctcttcactcatctgactgc caatggtgcc 600 tttccaaatg gtatcgttgc agttgtctca gtcatgttggctgttaacta tgccttctct 660 ggtactgagt taattggtat tgcggctggt gaaacggataatcccaaaga agctgtacca 720 agggctatta aaacgacaat cggtcgcttg gttgttttctttgtactgac aattgttgtc 780 ctagcttcgc tattgccaat gaaagaggca ggcgtatccacagcaccatt cgttgatgtc 840 tttgacaaga tgggaatccc ttttacggcg gatatcatgaacttcgttat cttgacagcc 900 atcctgtctg ctggtaactc aggtctctac gcatcaagccgtatgctctg gtcccttgcc 960 aatgaaggta tgttgtcaaa atctgttgtg aaaatcaataaacacggtgt cccaatgcgt 1020 gctcttctct tgtcaatggc aggagcagtg ctgtcgctcttttcaagtat ttacgctgca 1080 gacacagttt atctagcctt ggtttcaatc gcgggctttgctgttgttgt cgtatggcta 1140 gccattccag tcgcacaaat caatttccgc aaggaattc1179 224 393 PRT Streptococcus agalactiae 224 Met Glu Asn His Arg TyrGlu Asp Glu Gly Lys Phe Gln Arg Lys Met 1 5 10 15 Thr Ser Arg His LeuPhe Met Leu Ser Leu Gly Gly Val Ile Gly Thr 20 25 30 Gly Leu Phe Leu SerSer Gly Tyr Thr Ile Ala Gln Ala Gly Pro Leu 35 40 45 Gly Ala Val Leu SerTyr Leu Ile Gly Ala Val Val Val Tyr Leu Val 50 55 60 Met Leu Ser Leu GlyGlu Leu Ala Val Ala Met Pro Val Thr Gly Ser 65 70 75 80 Phe His Thr TyrAla Thr Lys Phe Ile Ser Pro Gly Thr Gly Phe Thr 85 90 95 Val Ala Trp LeuTyr Trp Ile Cys Trp Thr Val Ala Leu Gly Thr Glu 100 105 110 Phe Leu GlyAla Ala Met Leu Met Gln Arg Trp Phe Pro Asn Val Pro 115 120 125 Ala TrpAla Phe Ala Ser Phe Phe Ala Leu Val Ile Phe Gly Leu Asn 130 135 140 AlaLeu Ser Val Arg Phe Phe Ala Glu Ala Glu Ser Phe Phe Ser Ser 145 150 155160 Ile Lys Val Ile Ala Ile Ile Ile Phe Ile Ile Leu Gly Leu Gly Ala 165170 175 Met Phe Gly Leu Val Ser Phe Glu Gly Gln His Lys Ala Ile Leu Phe180 185 190 Thr His Leu Thr Ala Asn Gly Ala Phe Pro Asn Gly Ile Val AlaVal 195 200 205 Val Ser Val Met Leu Ala Val Asn Tyr Ala Phe Ser Gly ThrGlu Leu 210 215 220 Ile Gly Ile Ala Ala Gly Glu Thr Asp Asn Pro Lys GluAla Val Pro 225 230 235 240 Arg Ala Ile Lys Thr Thr Ile Gly Arg Leu ValVal Phe Phe Val Leu 245 250 255 Thr Ile Val Val Leu Ala Ser Leu Leu ProMet Lys Glu Ala Gly Val 260 265 270 Ser Thr Ala Pro Phe Val Asp Val PheAsp Lys Met Gly Ile Pro Phe 275 280 285 Thr Ala Asp Ile Met Asn Phe ValIle Leu Thr Ala Ile Leu Ser Ala 290 295 300 Gly Asn Ser Gly Leu Tyr AlaSer Ser Arg Met Leu Trp Ser Leu Ala 305 310 315 320 Asn Glu Gly Met LeuSer Lys Ser Val Val Lys Ile Asn Lys His Gly 325 330 335 Val Pro Met ArgAla Leu Leu Leu Ser Met Ala Gly Ala Val Leu Ser 340 345 350 Leu Phe SerSer Ile Tyr Ala Ala Asp Thr Val Tyr Leu Ala Leu Val 355 360 365 Ser IleAla Gly Phe Ala Val Val Val Val Trp Leu Ala Ile Pro Val 370 375 380 AlaGln Ile Asn Phe Arg Lys Glu Phe 385 390 225 636 DNA Streptococcusagalactiae 225 tcagaaaatg cagaggcagc aacggttgcc acaaacttgg ttaccaaaggagctaatgtc 60 attatcggac cagcaacatc gggtgcagct gcatcttcaa ctccaaaagtaaatgcagca 120 gcagttccaa tgattgcacc tgctgcgaca caagacaatt tagtctatggttctgatgga 180 aaaaccttaa atcagtattt cttccgagct acttttgtcg ataattatcaaggaaagcta 240 ttgtctcagt atgctacaga caaccttaaa gctaaaaaag ttgttctattttatgataat 300 tcatcagatt actcaaaggg ggtagcaaaa tcatttaagg aaagttatagtggaaaaatt 360 gttgatagta tgacattctc cgctggtgat actgatttcc aagcgtcattgactaagttg 420 aaagggaaag aatatgatgc tattgtgatg ccaggttact ataccgagacaggattaata 480 gttaagcaag cgcgtgattt aggtatctct aaaccggttc ttgggcctgatggttttgat 540 agtccgaaat ttgtgcaatc ggcaacacct gtaggagctt caaacgtttattatttgaca 600 ggtttcacta cacaaggatc aaccaaagct aaagct 636 226 212 PRTStreptococcus agalactiae 226 Ser Glu Asn Ala Glu Ala Ala Thr Val Ala ThrAsn Leu Val Thr Lys 1 5 10 15 Gly Ala Asn Val Ile Ile Gly Pro Ala ThrSer Gly Ala Ala Ala Ser 20 25 30 Ser Thr Pro Lys Val Asn Ala Ala Ala ValPro Met Ile Ala Pro Ala 35 40 45 Ala Thr Gln Asp Asn Leu Val Tyr Gly SerAsp Gly Lys Thr Leu Asn 50 55 60 Gln Tyr Phe Phe Arg Ala Thr Phe Val AspAsn Tyr Gln Gly Lys Leu 65 70 75 80 Leu Ser Gln Tyr Ala Thr Asp Asn LeuLys Ala Lys Lys Val Val Leu 85 90 95 Phe Tyr Asp Asn Ser Ser Asp Tyr SerLys Gly Val Ala Lys Ser Phe 100 105 110 Lys Glu Ser Tyr Ser Gly Lys IleVal Asp Ser Met Thr Phe Ser Ala 115 120 125 Gly Asp Thr Asp Phe Gln AlaSer Leu Thr Lys Leu Lys Gly Lys Glu 130 135 140 Tyr Asp Ala Ile Val MetPro Gly Tyr Tyr Thr Glu Thr Gly Leu Ile 145 150 155 160 Val Lys Gln AlaArg Asp Leu Gly Ile Ser Lys Pro Val Leu Gly Pro 165 170 175 Asp Gly PheAsp Ser Pro Lys Phe Val Gln Ser Ala Thr Pro Val Gly 180 185 190 Ala SerAsn Val Tyr Tyr Leu Thr Gly Phe Thr Thr Gln Gly Ser Thr 195 200 205 LysAla Lys Ala 210 227 270 DNA Streptococcus agalactiae 227 ttgggacttaaagaccatgc tttagtctat ccattttcat tatctggggg gcaaaagcaa 60 cgtgtcgcactagctcgtgc gatgatgatt gatccacaga ttattggtta tgatgagcca 120 actagcgctcttgatccaga gttgcgtcaa gaagtagaaa aactaatttt acaaaataga 180 gaaacaggtatgacacaaat tgtagtaaca catgatcttc aatttgctga aagtatatct 240 gatacgattctcaaaattaa tcctaagtag 270 228 89 PRT Streptococcus agalactiae 228 MetGly Leu Lys Asp His Ala Leu Val Tyr Pro Phe Ser Leu Ser Gly 1 5 10 15Gly Gln Lys Gln Arg Val Ala Leu Ala Arg Ala Met Met Ile Asp Pro 20 25 30Gln Ile Ile Gly Tyr Asp Glu Pro Thr Ser Ala Leu Asp Pro Glu Leu 35 40 45Arg Gln Glu Val Glu Lys Leu Ile Leu Gln Asn Arg Glu Thr Gly Met 50 55 60Thr Gln Ile Val Val Thr His Asp Leu Gln Phe Ala Glu Ser Ile Ser 65 70 7580 Asp Thr Ile Leu Lys Ile Asn Pro Lys 85 229 204 DNA Streptococcusagalactiae 229 atgactaata tctcagatgt tccaaaagct attagaacac aggcacagtatgttctcttg 60 ggaatgagag ttatggatca gtcggtatta ccgaaaacat ataattcaaaagaaccttat 120 ttgaaaccag atatgattta tattcatgat agaagacaag agacaatgcttaaaatcact 180 caagaaatag aaatggagca ttga 204 230 67 PRT Streptococcusagalactiae 230 Met Thr Asn Ile Ser Asp Val Pro Lys Ala Ile Arg Thr GlnAla Gln 1 5 10 15 Tyr Val Leu Leu Gly Met Arg Val Met Asp Gln Ser ValLeu Pro Lys 20 25 30 Thr Tyr Asn Ser Lys Glu Pro Tyr Leu Lys Pro Asp MetIle Tyr Ile 35 40 45 His Asp Arg Arg Gln Glu Thr Met Leu Lys Ile Thr GlnGlu Ile Glu 50 55 60 Met Glu His 65 231 1411 DNA Streptococcusagalactiae 231 aagcttgcat gcctgcaggt cgactctaga ggatcttggg gaatataaatttggatttca 60 tgacgatgta aagccaattt attctacggg aaaaggtcta aatgaggctgttattcgtga 120 gttatctgca gctaagggtg aacctgagtg gatgttggac tttcgtctaaaatccttgga 180 aacgtttaat aaaatgccga tgcagacctg gggagcagat ttatcagatattgattttga 240 tgatattatt tattatcaaa aagcatctga taaacctgcg cgtgattgggatgatgttcc 300 agaaaaaatc aaagaaactt ttgaaagaat tgggattcca gaagctgaaagagcctatct 360 tgcaggagca tcagcacaat atgaatcaga agtagtttat cacaatatgaaagaagaata 420 tgataagctg ggtattgttt ttacggatac tgactctgca cttaaagagtacccagagct 480 attcaaaaaa tattttgcta aacttgtccc tccaacagat aataaattagctgctctgaa 540 ctctgctgta tggtcaggtg gaacatttat ttatgttcct aaaggtgttaaggtggatat 600 tccacttcaa acttacttcc gtattaataa tgaaaatact ggacaatttgaacgtactct 660 cattattgtt gatgagggag caagtgttca ctatgttgaa ggttgtaccgccccaactta 720 ttcttcaaat agtttacatg cagctatagt tgaaattttt gcacttgatggagcttatat 780 gcgctatacg actattcaaa attggtccga taatgtctat aatttagtgacaaaacgtgc 840 taccgctaaa aaagatgcaa cagttgagtg gatagatgga aatctaggagctaaaacaac 900 aatgaaatac ccatcggttt accttgatgg tgaaggagca cgtggcacgatgttgtctat 960 tgcttttgca aacaaaggac aacaccaaga tacgggtgca aagatgattcataatgcccc 1020 ccatactagt tcatccattg tctctaaatc aattgctaag ggtgggggaaaagttgatta 1080 tcgaggtcaa gtgacattta ataaagattc caaaaaatca gtgtcacatatagaatgtga 1140 caccatattg atggatgata tttcaaaatc agataccata ccgtttaatgagattcataa 1200 ttcacaggtt gctttagagc atgaagcaaa ggtgtctaag atttctgaagagcaactgta 1260 ctacttgatg agtcgaggtt tatctgaagc tgaagcaaca gaaatgattgttatggggtt 1320 tgttgagccc tttacgaaag aattaccaat ggaatatgcg gtagagttaaatcgtttaat 1380 ttcctatgaa atggaaggtt cagttggtta a 1411 232 468 PRTStreptococcus agalactiae 232 Met His Ala Cys Arg Ser Thr Leu Glu Asp LeuGly Glu Tyr Lys Phe 1 5 10 15 Gly Phe His Asp Asp Val Lys Pro Ile TyrSer Thr Gly Lys Gly Leu 20 25 30 Asn Glu Ala Val Ile Arg Glu Leu Ser AlaAla Lys Gly Glu Pro Glu 35 40 45 Trp Met Leu Asp Phe Arg Leu Lys Ser LeuGlu Thr Phe Asn Lys Met 50 55 60 Pro Met Gln Thr Trp Gly Ala Asp Leu SerAsp Ile Asp Phe Asp Asp 65 70 75 80 Ile Ile Tyr Tyr Gln Lys Ala Ser AspLys Pro Ala Arg Asp Trp Asp 85 90 95 Asp Val Pro Glu Lys Ile Lys Glu ThrPhe Glu Arg Ile Gly Ile Pro 100 105 110 Glu Ala Glu Arg Ala Tyr Leu AlaGly Ala Ser Ala Gln Tyr Glu Ser 115 120 125 Glu Val Val Tyr His Asn MetLys Glu Glu Tyr Asp Lys Leu Gly Ile 130 135 140 Val Phe Thr Asp Thr AspSer Ala Leu Lys Glu Tyr Pro Glu Leu Phe 145 150 155 160 Lys Lys Tyr PheAla Lys Leu Val Pro Pro Thr Asp Asn Lys Leu Ala 165 170 175 Ala Leu AsnSer Ala Val Trp Ser Gly Gly Thr Phe Ile Tyr Val Pro 180 185 190 Lys GlyVal Lys Val Asp Ile Pro Leu Gln Thr Tyr Phe Arg Ile Asn 195 200 205 AsnGlu Asn Thr Gly Gln Phe Glu Arg Thr Leu Ile Ile Val Asp Glu 210 215 220Gly Ala Ser Val His Tyr Val Glu Gly Cys Thr Ala Pro Thr Tyr Ser 225 230235 240 Ser Asn Ser Leu His Ala Ala Ile Val Glu Ile Phe Ala Leu Asp Gly245 250 255 Ala Tyr Met Arg Tyr Thr Thr Ile Gln Asn Trp Ser Asp Asn ValTyr 260 265 270 Asn Leu Val Thr Lys Arg Ala Thr Ala Lys Lys Asp Ala ThrVal Glu 275 280 285 Trp Ile Asp Gly Asn Leu Gly Ala Lys Thr Thr Met LysTyr Pro Ser 290 295 300 Val Tyr Leu Asp Gly Glu Gly Ala Arg Gly Thr MetLeu Ser Ile Ala 305 310 315 320 Phe Ala Asn Lys Gly Gln His Gln Asp ThrGly Ala Lys Met Ile His 325 330 335 Asn Ala Pro His Thr Ser Ser Ser IleVal Ser Lys Ser Ile Ala Lys 340 345 350 Gly Gly Gly Lys Val Asp Tyr ArgGly Gln Val Thr Phe Asn Lys Asp 355 360 365 Ser Lys Lys Ser Val Ser HisIle Glu Cys Asp Thr Ile Leu Met Asp 370 375 380 Asp Ile Ser Lys Ser AspThr Ile Pro Phe Asn Glu Ile His Asn Ser 385 390 395 400 Gln Val Ala LeuGlu His Glu Ala Lys Val Ser Lys Ile Ser Glu Glu 405 410 415 Gln Leu TyrTyr Leu Met Ser Arg Gly Leu Ser Glu Ala Glu Ala Thr 420 425 430 Glu MetIle Val Met Gly Phe Val Glu Pro Phe Thr Lys Glu Leu Pro 435 440 445 MetGlu Tyr Ala Val Glu Leu Asn Arg Leu Ile Ser Tyr Glu Met Glu 450 455 460Gly Ser Val Gly 465 233 261 DNA Streptococcus agalactiae 233 atgatagaattcttttctaa tatcagaaca gagattccgc agatgccttt acttatccat 60 agtttgattttatctgtctt accttttctg atgtggctga ctttggttaa tagagataag 120 cctttgtataaaactatttg gagtatcctt ttaggacttc agttaattac gatttatact 180 tggtttttctgggcaaaatt gcctttatct gaaagtcttc ccctttacca ttgtcgaata 240 ggcatgtttgtcggtctctt a 261 234 87 PRT Streptococcus agalactiae 234 Met Ile Glu PhePhe Ser Asn Ile Arg Thr Glu Ile Pro Gln Met Pro 1 5 10 15 Leu Leu IleHis Ser Leu Ile Leu Ser Val Leu Pro Phe Leu Met Trp 20 25 30 Leu Thr LeuVal Asn Arg Asp Lys Pro Leu Tyr Lys Thr Ile Trp Ser 35 40 45 Ile Leu LeuGly Leu Gln Leu Ile Thr Ile Tyr Thr Trp Phe Phe Trp 50 55 60 Ala Lys LeuPro Leu Ser Glu Ser Leu Pro Leu Tyr His Cys Arg Ile 65 70 75 80 Gly MetPhe Val Gly Leu Leu 85 235 486 DNA Streptococcus agalactiae 235aagcttgtgc aaagtattaa agagatagga ttagctaatg cgcatttatt agctgttgct 60ccgacagggt caatcagtta tctttcttct tgtactccga gccttcaacc ggttgtatca 120cctgtcgaag tacgcaagga aggagcactg gggagggttt atgtagctgc ttataagatt 180gatgcagata attatgtcta ctacaaaaaa ggagcttatg aagtgggatc tgaggcgatt 240atcaatattg cagctgccgc tcaaaaacac attgatcaag ctatttcgtt aacgcttttc 300atgacagatc aagcaactac gcgagattta aataaagcct atattcaagc atttaaacaa 360aaatgtgcct ctatttatta tgtacgagtg agacaggaca tcctagaagg tagcgagagt 420tatgatgata tgctggatga tttcacttca tcggacttag aagactgtca atcctgcatg 480atttaa 486 236 161 PRT Streptococcus agalactiae 236 Lys Leu Val Gln SerIle Lys Glu Ile Gly Leu Ala Asn Ala His Leu 1 5 10 15 Leu Ala Val AlaPro Thr Gly Ser Ile Ser Tyr Leu Ser Ser Cys Thr 20 25 30 Pro Ser Leu GlnPro Val Val Ser Pro Val Glu Val Arg Lys Glu Gly 35 40 45 Ala Leu Gly ArgVal Tyr Val Ala Ala Tyr Lys Ile Asp Ala Asp Asn 50 55 60 Tyr Val Tyr TyrLys Lys Gly Ala Tyr Glu Val Gly Ser Glu Ala Ile 65 70 75 80 Ile Asn IleAla Ala Ala Ala Gln Lys His Ile Asp Gln Ala Ile Ser 85 90 95 Leu Thr LeuPhe Met Thr Asp Gln Ala Thr Thr Arg Asp Leu Asn Lys 100 105 110 Ala TyrIle Gln Ala Phe Lys Gln Lys Cys Ala Ser Ile Tyr Tyr Val 115 120 125 ArgVal Arg Gln Asp Ile Leu Glu Gly Ser Glu Ser Tyr Asp Asp Met 130 135 140Leu Asp Asp Phe Thr Ser Ser Asp Leu Glu Asp Cys Gln Ser Cys Met 145 150155 160 Ile 237 413 DNA Streptococcus agalactiae 237 gtgaggacatatattacaaa cttgaatgga cattcaatca ctagtacagc acaaatagct 60 caaaacatggtaacagatat agcagtaagc ttaggttttc gtgagctggg aatacattct 120 tatccgattgatactgattc tcctgaggaa atgagtaagc gtttagatgg aatctgttcc 180 ggacttagaaaaaatgatat tgtcatattt cagacaccta catggaacac tacaactttt 240 gatgaaaaattatttcacaa attaaaaata tttggtgtaa agattgttat ttttatacat 300 gatgttgtaccgctaatgtt tgatggaaat ttttatttga tggatagaac tatagcttat 360 tataatgaagcagatgttta atagccccta gtcaagcaat ggtcgataag ctt 413 238 138 PRTStreptococcus agalactiae 238 Met Arg Thr Tyr Ile Thr Asn Leu Asn Gly HisSer Ile Thr Ser Thr 1 5 10 15 Ala Gln Ile Ala Gln Asn Met Val Thr AspIle Ala Val Ser Leu Gly 20 25 30 Phe Arg Glu Leu Gly Ile His Ser Tyr ProIle Asp Thr Asp Ser Pro 35 40 45 Glu Glu Met Ser Lys Arg Leu Asp Gly IleCys Ser Gly Leu Arg Lys 50 55 60 Asn Asp Ile Val Ile Phe Gln Thr Pro ThrTrp Asn Thr Thr Thr Phe 65 70 75 80 Asp Glu Lys Leu Phe His Lys Leu LysIle Phe Gly Val Lys Ile Val 85 90 95 Ile Phe Ile His Asp Val Val Pro LeuMet Phe Asp Gly Asn Phe Tyr 100 105 110 Leu Met Asp Arg Thr Ile Ala TyrTyr Asn Glu Ala Asp Val Leu Ile 115 120 125 Ala Pro Ser Gln Ala Met ValAsp Lys Leu 130 135 239 261 DNA Streptococcus agalactiae 239 catggaaatgaagttgatga tgttattaga agggcatttg aatataatca ccttatcttt 60 gcttttgataatacctgtca taacagagag ttagtattag atagcaatat catttctcac 120 acaacctgtgaacaattgat aaatttaatg aaaaatttat caggctccat tatgtatttg 180 ctagagcaacaaagagaaca aacaagtaat gaaacaaaag agcgttataa agaaatatta 240 ggagggtatggaaatgccta a 261 240 86 PRT Streptococcus agalactiae 240 His Gly Asn GluVal Asp Asp Val Ile Arg Arg Ala Phe Glu Tyr Asn 1 5 10 15 His Leu IlePhe Ala Phe Asp Asn Thr Cys His Asn Arg Glu Leu Val 20 25 30 Leu Asp SerAsn Ile Ile Ser His Thr Thr Cys Glu Gln Leu Ile Asn 35 40 45 Leu Met LysAsn Leu Ser Gly Ser Ile Met Tyr Leu Leu Glu Gln Gln 50 55 60 Arg Glu GlnThr Ser Asn Glu Thr Lys Glu Arg Tyr Lys Glu Ile Leu 65 70 75 80 Gly GlyTyr Gly Asn Ala 85 241 312 DNA Streptococcus agalactiae 241 acatttttatattatgtatt tgaagacgta gccacccagt caaatatgac tgggaagatt 60 tttagtatgtctaaagaaga gttgtcatat ttacccgtta ttaaactttt taagaatcaa 120 ggtgtatacaacggcttgat tggtctattc ctcctttatg ggttatatat ttcacagaat 180 caagaaattgtagctatttt tttaatcaat gtgttgctag ttgctgttta tggtgctttg 240 acagttgataaaaaaatctt attaaaacag ggtggtttac ctatattagc tcttttaaca 300 ttcttatttt aa312 242 103 PRT Streptococcus agalactiae 242 Thr Phe Leu Tyr Tyr Val PheGlu Asp Val Ala Thr Gln Ser Asn Met 1 5 10 15 Thr Gly Lys Ile Phe SerMet Ser Lys Glu Glu Leu Ser Tyr Leu Pro 20 25 30 Val Ile Lys Leu Phe LysAsn Gln Gly Val Tyr Asn Gly Leu Ile Gly 35 40 45 Leu Phe Leu Leu Tyr GlyLeu Tyr Ile Ser Gln Asn Gln Glu Ile Val 50 55 60 Ala Ile Phe Leu Ile AsnVal Leu Leu Val Ala Val Tyr Gly Ala Leu 65 70 75 80 Thr Val Asp Lys LysIle Leu Leu Lys Gln Gly Gly Leu Pro Ile Leu 85 90 95 Ala Leu Leu Thr PheLeu Phe 100 243 588 DNA Streptococcus agalactiae 243 atgaaattaagtgtccttga ttatgggctt attgattatg gaaaaactgc aagtgatgca 60 atacaagaaacgattctttt atcacaagag gcggagcaac taggctatca tcaattttgg 120 gtggctgaacatcacggtgt taaggcattc agtattagca atccagaatt aatgataatg 180 catttggctaaccagactaa atctatcaaa attggctctg gaggtataat gcctctgcac 240 tatagtagttttaaactcgc ggagactctc aagacattag agacatgtca tccgaatcga 300 gtaagtattggtttaggaaa ttcactaggg acagttaaag tttcaaatgc acttcgtagc 360 ttacataaagcacatgatta cgaagaggta ctggaggaat tgaagtcatg gcttattgat 420 gaatcatccagtaaggaacc attagttcaa ccgactcttt ctagcttccc agacttatat 480 gtgttggggagtggtcaaaa atcagcttat ttagcggcta aacttggctt aggctttacc 540 ttcggtgtttttccttttat ggacaaagac ccattgacag aagctaaa 588 244 196 PRT Streptococcusagalactiae 244 Met Lys Leu Ser Val Leu Asp Tyr Gly Leu Ile Asp Tyr GlyLys Thr 1 5 10 15 Ala Ser Asp Ala Ile Gln Glu Thr Ile Leu Leu Ser GlnGlu Ala Glu 20 25 30 Gln Leu Gly Tyr His Gln Phe Trp Val Ala Glu His HisGly Val Lys 35 40 45 Ala Phe Ser Ile Ser Asn Pro Glu Leu Met Ile Met HisLeu Ala Asn 50 55 60 Gln Thr Lys Ser Ile Lys Ile Gly Ser Gly Gly Ile MetPro Leu His 65 70 75 80 Tyr Ser Ser Phe Lys Leu Ala Glu Thr Leu Lys ThrLeu Glu Thr Cys 85 90 95 His Pro Asn Arg Val Ser Ile Gly Leu Gly Asn SerLeu Gly Thr Val 100 105 110 Lys Val Ser Asn Ala Leu Arg Ser Leu His LysAla His Asp Tyr Glu 115 120 125 Glu Val Leu Glu Glu Leu Lys Ser Trp LeuIle Asp Glu Ser Ser Ser 130 135 140 Lys Glu Pro Leu Val Gln Pro Thr LeuSer Ser Phe Pro Asp Leu Tyr 145 150 155 160 Val Leu Gly Ser Gly Gln LysSer Ala Tyr Leu Ala Ala Lys Leu Gly 165 170 175 Leu Gly Phe Thr Phe GlyVal Phe Pro Phe Met Asp Lys Asp Pro Leu 180 185 190 Thr Glu Ala Lys 195245 40 DNA Artificial Sequence Primer 245 cgagatctga tatctcacaaacagataacg gcgtaaatag 40 246 43 DNA Artificial Sequence Primer 246gaagatcttc cccgggatca caaacagata acggcgtaaa tag 43 247 42 DNA ArtificialSequence Primer 247 cgagatctga tatccatcac aaacagataa cggcgtaaat ag 42248 32 DNA Artificial Sequence Primer 248 cgggatcctt atggacctgaatcagcgttg tc 32 249 23 DNA Artificial Sequence Primer 249 ggatgctttgtttcaggtgt atc 23 250 82 DNA Artificial Sequence Primer 250 catgatatcggtacctcaag ctcatatcat tgtccggcaa tggtgtgggc tttttttgtt 60 ttagcggataacaatttcac ac 82 251 81 DNA Artificial Sequence Primer 251 gcggatcccccgggcttaat taatgtttaa acactagtcg aagatctcgc gaattctcct 60 gtgtgaaattgttatccgct a 81 252 24 DNA Artificial Sequence Primer 252 cgccagggttttcccagtca cgac 24 253 20 DNA Artificial Sequence Primer 253 tcaggggggcggagcctatg 20 254 22 DNA Artificial Sequence Primer 254 tcgtatgttgtgtggaattg tg 22 255 26 DNA Artificial Sequence Primer 255 tccggctcgtatgttgtgtg gaattg 26 256 43 DNA Artificial Sequence pTREP1-nuc1 vector256 aagtatcaga tctgatatct cacaaacaga taacggcgta aat 43 257 46 DNAArtificial Sequence pTREP1-nuc2 vector 257 aagtatcaga tcttccccgggatcacaaac agataacggc gtaaat 46 258 45 DNA Artificial SequencepTREP1-nuc3 vector 258 aagtatcaga tctgatatcc atcacaaaca gataacggcg taaat45 259 24 DNA Staphylococcus aureus 259 tcacaaacag ataacggcgt aaat 24260 17 DNA Artificial Sequence Primer 260 cgggatccgc caccatg 17 261 10DNA Artificial Sequence Primer 261 ttgcggccgc 10 262 38 DNA ArtificialSequence Primer 262 cggatccgcc accatggcgg atcaaactac atcggttc 38 263 36DNA Artificial Sequence Primer 263 ttgcggccgc gttgggataa ctagtcggtttagtcg 36 264 44 DNA Artificial Sequence Primer 264 cggatccgccaccatgaatc tttatttcca tagtactccc ttgc 44 265 37 DNA Artificial SequencePrimer 265 ttgcggccgc aaaatgatca gtttgagggt aaaagag 37 266 31 DNAArtificial Sequence Primer 266 catgccatgg cggatcaaac tacatcggtt c 31 26737 DNA Artificial Sequence Primer 267 catgccatgg caaaaatagt agtaccagtaatgcctc 37 268 32 DNA Artificial Sequence Primer 268 ttgcggccgcctctgaaata gtaatttgtc cg 32 269 35 DNA Artificial Sequence Primer 269catgccatgg gaaagaaagc aaataatgtc agtcc 35 270 31 DNA Artificial SequencePrimer 270 ttgcggccgc attgggtgta agcatttttt c 31 271 37 DNA ArtificialSequence Primer 271 catgccatgg gaactgagaa ctggttacat actaaag 37 272 33DNA Artificial Sequence Primer 272 ttgcggccgc attagctttt tcaacaattt ctc33 273 28 DNA Artificial Sequence Primer 273 ctagctagcc gatgtttgcgtgggaaag 28 274 40 DNA Artificial Sequence Primer 274 ttgcggccgcataagattta acaataccaa gtaatatagc 40 275 39 DNA Artificial SequencePrimer 275 ggggtaccgg ccaccatggc tgaagtaatt tcaggaagt 39 276 39 DNAArtificial Sequence Primer 276 cggaattccg ttaatcctct ttttttcttagaaacagat 39

1. A Group B Streptococcus polypeptide or protein having a sequenceselected from those described in FIG. 1, or fragments or derivativesthereof.
 2. Derivatives or variants of the proteins, polypeptides, andpeptides as claimed in claim 1 which show at least 50% identity to thoseproteins, polypeptides and peptides claimed in claim
 1. 3. A Group BStreptococcus polypeptide or protein, or derivative or variant thereof,as claimed in claim 1 or claim 2, which is isolated or recombinant.
 4. Anucleic molecule comprising or consisting of a sequence which is: (i)any of the DNA sequences set out in FIG. 1 herein or their RNAequivalents; (ii) a sequence which is complementary to any of thesequences of (i); (iii) a sequence which codes for the same protein orpolypeptide, as those sequences of (i) or (ii); (iv) a sequence whichshows substantial identity with any of those of (i), (ii) and (iii); or(v) a sequence which codes for a derivative, or fragment of a nucleicacid molecule shown in FIG.
 1. 5. A vector comprising one or nucleicacid molecules as defined in claim
 4. 6. A vector as claimed in claim 4further comprising nucleic acid encoding any one or more of thefollowing: promoters, enhancers, signal sequences, leader sequences,translation start and stop signals, DNA stability controlling regions,or a fusion partner.
 7. The use of a vector as claimed in claim 5 orclaim 6 in the transformation or transfection of a prokaryotic oreukaryotic host.
 8. A host cell transformed with a vector as defined inclaim 5 or claim 6..
 9. A process for producing a Group B Streptococcuspolypeptide or protein, or derivative or variant thereof, as claimed inclaim 1 or claim 2, the process comprising expressing the polypeptide orprotein in a host cell as claimed in claim
 8. 10. An antibody, anaffibody, or a derivative thereof which binds to one or more of theproteins, polypeptides, peptides, fragments or derivatives thereof, asdefined in any one of claims 1 to
 3. 11. An immunogenic compositioncomprising one or more of the proteins, polypeptides, peptides,fragments or derivatives thereof as defined in any one of claims 1 to 3.12. An immunogenic composition as claimed in claim 11 wherein theproteins, polypeptides, peptides, or fragments or derivatives thereofinclude ID-65 or ID-83, ID-89, ID-93 or ID-96.
 13. An immunogeniccomposition as claimed in claim 11 or claim 12 which is a vaccine. 14.An immunogenic composition comprising one or more of the nucleic acidsequences as defined in claim
 4. 15. An immunogenic composition asclaimed in claim 14 wherein the nucleic acid sequences include ID-65 orID-66.
 16. An immunogenic composition as claimed in claim 14 or claim 15which is a vaccine.
 17. Use of an immunogenic composition as defined inany one of claims 11 to 16 in the preparation of a medicament for thetreatment or prophylaxis of Group B Streptococcus infection.
 18. Amethod of detection of Group B Streptococcus which comprises the step ofbringing into contact a sample to be tested with at least one antibody,affibody, or a derivative thereof, as defined in claim
 10. 19. A methodof detection of Group B Streptococcus which comprises the step ofbringing into contact a sample to be tested with at least one protein,polypeptide, peptide, fragments or derivatives as defined in any one ofclaims 1 to
 3. 20. A method of detection of Group B Streptococcus whichcomprises the step of bringing into contact a sample to be tested withat least one nucleic acid molecule as defined in claim
 4. 21. A kit forthe detection of Group B Streptococcus comprising at least one antibody,affibody, or derivatives thereof as defined in claim
 10. 22. A kit forthe detection of Group B Streptococcus comprising at least one Group BStreptococcus protein, polypeptide, peptide, fragment or derivativethereof as defined in any one of claims 1 to
 3. 23. A kit for thedetection of Group B Streptococcus comprising at least one nucleic acidmolecule as defined in claim
 4. 24. A method of determining whether aprotein, polypeptide, peptide, fragment or derivative thereof as definedin any one of claims 1 to 3 represents a potential anti-microbial targetwhich comprises inactivating said protein and determining whether GroupB Streptococcus is still viable.